LOCUS       NM_001403167            2476 bp    mRNA    linear   ROD 04-MAR-2025
DEFINITION  Mus musculus polymerase (DNA directed), iota (Poli), transcript
            variant 6, mRNA.
ACCESSION   NM_001403167 XM_006525945
VERSION     NM_001403167.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Cao,R., Liu,Y., Wei,K., Jin,N., Liang,Y., Ao,R., Pan,W., Wang,X.,
            Wang,X., Zhang,L. and Xie,J.
  TITLE     Genes related to neural tube defects and glioblastoma
  JOURNAL   Sci Rep 15 (1), 3777 (2025)
   PUBMED   39885289
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2476)
  AUTHORS   Gao,A., Zhang,M., Zhu,S.Q., Zou,S., Chen,H., Li,X., He,C., Zhou,L.,
            Mei,Y., Ding,W., Zhou,J., Zhou,Y. and Cao,Y.
  TITLE     DNA polymerase iota promotes EMT and metastasis of esophageal
            squamous cell carcinoma by interacting with USP7 to stabilize
            HIF-1alpha
  JOURNAL   Cell Death Dis 15 (2), 171 (2024)
   PUBMED   38402183
  REMARK    GeneRIF: DNA polymerase iota promotes EMT and metastasis of
            esophageal squamous cell carcinoma by interacting with USP7 to
            stabilize HIF-1alpha.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2476)
  AUTHORS   Kazachenko,K.Y., Miropolskaya,N.A., Gening,L.V., Tarantul,V.Z. and
            Makarova,A.V.
  TITLE     Alternative splicing at exon 2 results in the loss of the catalytic
            activity of mouse DNA polymerase iota in vitro
  JOURNAL   DNA Repair (Amst) 50, 77-82 (2017)
   PUBMED   28077248
  REMARK    GeneRIF: Exon 2 deletion abrogates both the DNA polymerase and dRP
            lyase activities of Pol iota in the presence of either Mg(2+) or
            Mn(2+) ions. Thus, 129-derived strains of mice express
            catalytically inactive alternatively spliced Pol iota variant.
REFERENCE   5  (bases 1 to 2476)
  AUTHORS   Frank,E.G., McDonald,J.P., Yang,W. and Woodgate,R.
  TITLE     Mouse DNA polymerase iota lacking the forty-two amino acids encoded
            by exon-2 is catalytically inactive in vitro
  JOURNAL   DNA Repair (Amst) 50, 71-76 (2017)
   PUBMED   28077247
  REMARK    GeneRIF: Mouse DNA polymerase iota lacking exon 2 (42 amino acids)
            is catalytically inactive in vitro.
REFERENCE   6  (bases 1 to 2476)
  AUTHORS   McIntyre,J., McLenigan,M.P., Frank,E.G., Dai,X., Yang,W., Wang,Y.
            and Woodgate,R.
  TITLE     Posttranslational Regulation of Human DNA Polymerase iota
  JOURNAL   J Biol Chem 290 (45), 27332-27344 (2015)
   PUBMED   26370087
REFERENCE   7  (bases 1 to 2476)
  AUTHORS   McDonald,J.P., Frank,E.G., Plosky,B.S., Rogozin,I.B., Masutani,C.,
            Hanaoka,F., Woodgate,R. and Gearhart,P.J.
  TITLE     129-derived strains of mice are deficient in DNA polymerase iota
            and have normal immunoglobulin hypermutation
  JOURNAL   J Exp Med 198 (4), 635-643 (2003)
   PUBMED   12925679
  REMARK    GeneRIF: Data suggest that either DNA polymerase iota does not
            participate in hypermutation, or its role is nonessential and can
            be readily assumed by another low-fidelity polymerase.
REFERENCE   8  (bases 1 to 2476)
  AUTHORS   Lee,G.H., Nishimori,H., Sasaki,Y., Matsushita,H., Kitagawa,T. and
            Tokino,T.
  TITLE     Analysis of lung tumorigenesis in chimeric mice indicates the
            Pulmonary adenoma resistance 2 (Par2) locus to operate in the
            tumor-initiation stage in a cell-autonomous manner: detection of
            polymorphisms in the Poli gene as a candidate for Par2
  JOURNAL   Oncogene 22 (15), 2374-2382 (2003)
   PUBMED   12700672
REFERENCE   9  (bases 1 to 2476)
  AUTHORS   Velasco-Miguel,S., Richardson,J.A., Gerlach,V.L., Lai,W.C., Gao,T.,
            Russell,L.D., Hladik,C.L., White,C.L. and Friedberg,E.C.
  TITLE     Constitutive and regulated expression of the mouse Dinb (Polkappa)
            gene encoding DNA polymerase kappa
  JOURNAL   DNA Repair (Amst) 2 (1), 91-106 (2003)
   PUBMED   12509270
REFERENCE   10 (bases 1 to 2476)
  AUTHORS   McDonald,J.P., Rapic-Otrin,V., Epstein,J.A., Broughton,B.C.,
            Wang,X., Lehmann,A.R., Wolgemuth,D.J. and Woodgate,R.
  TITLE     Novel human and mouse homologs of Saccharomyces cerevisiae DNA
            polymerase eta
  JOURNAL   Genomics 60 (1), 20-30 (1999)
   PUBMED   10458907
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC134831.3.
            
            On Mar 28, 2022 this sequence version replaced XM_006525945.5.
            
            Transcript Variant: This variant (6) encodes two different
            proteins, isoform 6 (long) and isoform 6 (short). Variant 6 encodes
            isoform 6 (long). However, this full length (736 aa) form is a
            minor form. A downstream translation start site is more commonly
            used, resulting in a 716 aa protein [isoform 6 (short)].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7345562.1865743.1,
                                           SRR13862062.51962.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164141 [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-134               AC134831.3         130779-130912       c
            135-257             AC134831.3         129293-129415       c
            258-422             AC134831.3         127148-127312       c
            423-575             AC134831.3         125967-126119       c
            576-812             AC134831.3         123754-123990       c
            813-991             AC134831.3         123293-123471       c
            992-1083            AC134831.3         122441-122532       c
            1084-1211           AC134831.3         117968-118095       c
            1212-1414           AC134831.3         117385-117587       c
            1415-2476           AC134831.3         109274-110335       c
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 44.48 cM"
     gene            1..2476
                     /gene="Poli"
                     /gene_synonym="Rad30b"
                     /note="polymerase (DNA directed), iota"
                     /db_xref="GeneID:26447"
                     /db_xref="MGI:MGI:1347081"
     exon            1..134
                     /gene="Poli"
                     /gene_synonym="Rad30b"
                     /inference="alignment:Splign:2.1.0"
     CDS             35..2245
                     /gene="Poli"
                     /gene_synonym="Rad30b"
                     /EC_number="2.7.7.7"
                     /note="isoform 6 (long) is encoded by transcript variant
                     6; RAD30 homolog B; DNA polymerase iota"
                     /codon_start=1
                     /product="DNA polymerase iota isoform 6 (long)"
                     /protein_id="NP_001390096.1"
                     /db_xref="GeneID:26447"
                     /db_xref="MGI:MGI:1347081"
                     /translation="MGVESEEEGGPAEEEDAPRAMEPLHAGAAGSSRVCSQGPPTQIS
                     SSRVIVHVDLDCFYAQVEMISNPELKDRPLGVQQKYLVVTCNYEARKLGVRKLMNVRD
                     AKEKCPQLVLVNGEDLSRYREMSYKVTELLEEFSPAVERLGFDENFVDLTEMVEKRLQ
                     QLPSEEVPSVTVFGHVYNNQSVNLHNIMHRRLVVGSQIAAEMREAMYNQLGLTGCAGV
                     APNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNHIKEIPGIGYKTAKRLEVLGINS
                     VHDLQTFPIKTLEKELGIAIAQRIQQLSFGEDKSPVTPSGPPQSFSEEDTFKKCSSEV
                     EAKAKIEELLSSLLTRVCQDGRKPHTVRLVIRRYSDKHCNRESRQCPIPSHVIQKLGT
                     GNHDSMPPLIDILMKLFRNMVNVKMPFHLTLMSVCFCNLKALSSAKKGPMDCYLTSLS
                     TPAYTDKRAFKVKDTHTEDSHKEKEANWDCLPSRRIESTGTGESPLDATCFPKEKDTS
                     DLPLQALPEGVDQEVFKQLPADIQEEILSGKSRENLKGKGSLSCPLHASRGVLSFFST
                     KQMQASRLSPRDTALPSKRVSAASPCEPGTSGLSPGSTSHPSCGKDCSYYIDSQLKDE
                     QTSQGPTESQGCQFSSTNPAVSGFHSFPNLQTEQLFSTHRTVDSHKQTATASHQGLES
                     HQGLESRELDSAEEKLPFPPDIDPQVFYELPEEVQKELMAEWERAGAARPSAHR"
     exon            135..257
                     /gene="Poli"
                     /gene_synonym="Rad30b"
                     /inference="alignment:Splign:2.1.0"
     exon            258..422
                     /gene="Poli"
                     /gene_synonym="Rad30b"
                     /inference="alignment:Splign:2.1.0"
     exon            423..575
                     /gene="Poli"
                     /gene_synonym="Rad30b"
                     /inference="alignment:Splign:2.1.0"
     exon            576..812
                     /gene="Poli"
                     /gene_synonym="Rad30b"
                     /inference="alignment:Splign:2.1.0"
     exon            813..991
                     /gene="Poli"
                     /gene_synonym="Rad30b"
                     /inference="alignment:Splign:2.1.0"
     exon            992..1083
                     /gene="Poli"
                     /gene_synonym="Rad30b"
                     /inference="alignment:Splign:2.1.0"
     exon            1084..1211
                     /gene="Poli"
                     /gene_synonym="Rad30b"
                     /inference="alignment:Splign:2.1.0"
     exon            1212..1414
                     /gene="Poli"
                     /gene_synonym="Rad30b"
                     /inference="alignment:Splign:2.1.0"
     exon            1415..2476
                     /gene="Poli"
                     /gene_synonym="Rad30b"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2452..2457
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Poli"
                     /gene_synonym="Rad30b"
                     /note="hexamer: AATAAA"
     polyA_site      2474
                     /gene="Poli"
                     /gene_synonym="Rad30b"
                     /note="major polyA site"
ORIGIN      
        1 gcagcagctg cggtgcggtt ggcccgaagg gaagatgggg gtggagtcgg aggaggaagg
       61 cggccccgcg gaggaagaag acgctcctcg tgccatggag cccttgcacg cgggggcggc
      121 gggcagctcg cgggtttgca gtcaagggcc acctacacaa atctcttcgt cgagagtcat
      181 agtccacgta gatctggatt gcttttatgc ccaggtcgaa atgatctcca acccagaatt
      241 aaaggacaga cctttaggtg tgcaacagaa atacttggtg gttacctgca actatgaagc
      301 caggaaactg ggtgtgagga agcttatgaa tgtgagagat gcgaaggaaa agtgtcctca
      361 gctggtcctc gtgaacggag aagacctgag ccgctacaga gagatgtcct ataaggtcac
      421 agaattgttg gaggaattta gtccagctgt tgagagactt ggatttgatg aaaattttgt
      481 ggatctaaca gaaatggtgg aaaaaagact ccagcagctt ccaagtgagg aggttccgtc
      541 agtgactgtg ttcggccatg tttacaataa ccagtctgtg aacctacaca acatcatgca
      601 tcggagactt gtggttggat cgcagattgc agcagagatg cgggaagcca tgtataatca
      661 gctcgggctc acaggctgcg ccggagtggc tcctaataaa ctcttggcaa agttagtgtc
      721 tggggttttt aaaccaaatc agcagacggt cttactacct gaaagttgtc aacatctcat
      781 tcacagtttg aaccacataa aggaaattcc tggtatcggc tataaaactg ccaagcgtct
      841 cgaagttctg ggaatcaata gtgttcatga tctccaaacc tttccaatca aaacattaga
      901 aaaagaatta ggaattgcaa ttgctcagcg tatccagcag ctcagttttg gagaggacaa
      961 gtctcccgtc acaccgtcag ggccaccaca gtcctttagt gaagaagata cgtttaaaaa
     1021 gtgttcctca gaagtggaag ctaaagctaa gattgaagaa ttactttcca gccttttgac
     1081 cagagtatgc caggatggaa ggaagcccca tacagtaaga ttagtcatcc gtcggtactc
     1141 tgacaaacac tgtaatcgag agagtcgtca gtgccctatc ccatcccacg tcattcagaa
     1201 gttagggaca ggaaatcatg actccatgcc tcccctgatt gatatcctta tgaaactttt
     1261 ccgaaatatg gtgaacgtga agatgccctt tcacctgact cttatgagcg tgtgcttctg
     1321 caacctgaaa gccctgagca gtgctaagaa agggcctatg gactgctacc taacgtccct
     1381 ctcaacccct gcctacaccg acaagcgcgc ctttaaagtg aaggacaccc acacggaaga
     1441 ctctcacaag gagaaagaag caaactggga ttgtctacca agtagaagaa tcgaaagcac
     1501 aggaacgggg gagtctccgt tggatgccac ctgttttcct aaagaaaaag acacaagtga
     1561 cttgcccctc caggcactgc cagagggtgt tgatcaagaa gtctttaagc aacttccagc
     1621 agatattcaa gaagaaatcc tttctggaaa atctagagaa aatctaaaag ggaaaggaag
     1681 tttaagttgt ccgctgcatg cctctagagg agtattgtct ttcttttcta caaagcaaat
     1741 gcaagccagt cgcttaagcc ccagagatac tgcgctccct agcaagcggg tatcagctgc
     1801 atctccctgt gagccgggaa cgtcaggact gagccccggg agcacctccc atccatcgtg
     1861 cggaaaggac tgttcctatt acatagacag ccagttaaag gatgagcaaa cgagtcaagg
     1921 ccctactgag tctcaaggat gccagttttc cagcacgaac cctgctgttt ctggtttcca
     1981 ttcctttcct aatctgcaga ctgaacagct cttctccaca catcgcactg tagacagcca
     2041 caagcagaca gccaccgcct ctcaccaggg actagaaagt caccagggac tagaaagcag
     2101 agagctggat tctgcggaag aaaagcttcc tttcccacca gacattgatc cgcaagtttt
     2161 ctatgagctt ccagaagagg tccaaaagga actgatggcg gaatgggaga gagctggagc
     2221 tgcgcgcccc tcggcgcaca gataagtcca gaaagccagg gagtatgagt ccccaggtcg
     2281 ccagggcata accaaataga aatagtcagt ggtgtggaaa tccgatgtaa gatgtaatag
     2341 ataaggcaaa agtgggcaca ggaaggaaat tctagtacaa agggaaaata gccataatgt
     2401 ggtaatgtaa aaaatgctat ctccctatta tttctgtttt atatcattgt caataaaagg
     2461 aatatcatgg tcaaca
//
