LOCUS       NM_030715               3234 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus polymerase (DNA directed), eta (RAD 30 related)
            (Polh), transcript variant 1, mRNA.
ACCESSION   NM_030715 XM_001002709
VERSION     NM_030715.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Zhou,L., Dai,H., Wu,J., Zhou,M., Yuan,H., Du,J., Yang,L., Wu,X.,
            Xu,H., Hua,Y., Xu,J., Zheng,L. and Shen,B.
  TITLE     Role of FEN1 S187 phosphorylation in counteracting oxygen-induced
            stress and regulating postnatal heart development
  JOURNAL   FASEB J 31 (1), 132-147 (2017)
   PUBMED   27694478
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Fritzen,R., Delbos,F., De Smet,A., Palancade,B., Canman,C.E.,
            Aoufouchi,S., Weill,J.C., Reynaud,C.A. and Storck,S.
  TITLE     A single aspartate mutation in the conserved catalytic site of
            Rev3L generates a hypomorphic phenotype in vivo and in vitro
  JOURNAL   DNA Repair (Amst) 46, 37-46 (2016)
   PUBMED   27481099
REFERENCE   3  (bases 1 to 3234)
  AUTHORS   Lange,S.S., Tomida,J., Boulware,K.S., Bhetawal,S. and Wood,R.D.
  TITLE     The Polymerase Activity of Mammalian DNA Pol zeta Is Specifically
            Required for Cell and Embryonic Viability
  JOURNAL   PLoS Genet 12 (1), e1005759 (2016)
   PUBMED   26727495
  REMARK    GeneRIF: the functions of REV3L in maintaining cell viability,
            embryonic viability and genomic stability are directly dependent on
            its polymerase activity, and cannot be ameliorated by an additional
            deletion of pol eta.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3234)
  AUTHORS   Chen,Y.W., Harris,R.A., Hatahet,Z. and Chou,K.M.
  TITLE     Ablation of XP-V gene causes adipose tissue senescence and
            metabolic abnormalities
  JOURNAL   Proc Natl Acad Sci U S A 112 (33), E4556-E4564 (2015)
   PUBMED   26240351
  REMARK    GeneRIF: XP-V has a role in adipose tissue senescence and metabolic
            syndrome
REFERENCE   5  (bases 1 to 3234)
  AUTHORS   Kanao,R., Yokoi,M., Ohkumo,T., Sakurai,Y., Dotsu,K., Kura,S.,
            Nakatsu,Y., Tsuzuki,T., Masutani,C. and Hanaoka,F.
  TITLE     UV-induced mutations in epidermal cells of mice defective in DNA
            polymerase eta and/or iota
  JOURNAL   DNA Repair (Amst) 29, 139-146 (2015)
   PUBMED   25733082
  REMARK    GeneRIF: Increase in UV-induced mutations at both G:C and A:T pairs
            associated with PolH deficiency suggests that PolH contributes to
            accurate translesion synthesis (TLS) past both T- & C-containing
            dimers.
REFERENCE   6  (bases 1 to 3234)
  AUTHORS   Guo,C., Fischhaber,P.L., Luk-Paszyc,M.J., Masuda,Y., Zhou,J.,
            Kamiya,K., Kisker,C. and Friedberg,E.C.
  TITLE     Mouse Rev1 protein interacts with multiple DNA polymerases involved
            in translesion DNA synthesis
  JOURNAL   EMBO J 22 (24), 6621-6630 (2003)
   PUBMED   14657033
REFERENCE   7  (bases 1 to 3234)
  AUTHORS   Pavlov,Y.I., Rogozin,I.B., Galkin,A.P., Aksenova,A.Y., Hanaoka,F.,
            Rada,C. and Kunkel,T.A.
  TITLE     Correlation of somatic hypermutation specificity and A-T base pair
            substitution errors by DNA polymerase eta during copying of a mouse
            immunoglobulin kappa light chain transgene
  JOURNAL   Proc Natl Acad Sci U S A 99 (15), 9954-9959 (2002)
   PUBMED   12119399
  REMARK    GeneRIF: inaccurate DNA synthesis by mammalian DNA polymerase eta
            (pol eta) contributes to somatic hypermutation (SHM) of Ig genes
REFERENCE   8  (bases 1 to 3234)
  AUTHORS   Matsuda,T., Bebenek,K., Masutani,C., Rogozin,I.B., Hanaoka,F. and
            Kunkel,T.A.
  TITLE     Error rate and specificity of human and murine DNA polymerase eta
  JOURNAL   J Mol Biol 312 (2), 335-346 (2001)
   PUBMED   11554790
  REMARK    GeneRIF: DNA polymerase fidelity and specificity
REFERENCE   9  (bases 1 to 3234)
  AUTHORS   Yamada,A., Masutani,C., Iwai,S. and Hanaoka,F.
  TITLE     Complementation of defective translesion synthesis and UV light
            sensitivity in xeroderma pigmentosum variant cells by human and
            mouse DNA polymerase eta
  JOURNAL   Nucleic Acids Res 28 (13), 2473-2480 (2000)
   PUBMED   10871396
REFERENCE   10 (bases 1 to 3234)
  AUTHORS   Dai,G., Wang,D., Liu,B., Kasik,J.W., Muller,H., White,R.A.,
            Hummel,G.S. and Soares,M.J.
  TITLE     Three novel paralogs of the rodent prolactin gene family
  JOURNAL   J Endocrinol 166 (1), 63-75 (2000)
   PUBMED   10856884
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ048830.1, AK036296.1 and AC116739.13.
            
            On Jul 17, 2018 this sequence version replaced NM_030715.3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC049159.1, AK036296.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-16                CJ048830.1         2-17
            17-2596             AK036296.1         2-2581
            2597-3234           AC116739.13        121709-122346
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 22.9 cM"
     gene            1..3234
                     /gene="Polh"
                     /gene_synonym="RAD30A; XPV"
                     /note="polymerase (DNA directed), eta (RAD 30 related)"
                     /db_xref="GeneID:80905"
                     /db_xref="MGI:MGI:1891457"
     exon            1..245
                     /gene="Polh"
                     /gene_synonym="RAD30A; XPV"
                     /inference="alignment:Splign:2.1.0"
     exon            246..388
                     /gene="Polh"
                     /gene_synonym="RAD30A; XPV"
                     /inference="alignment:Splign:2.1.0"
     CDS             252..2336
                     /gene="Polh"
                     /gene_synonym="RAD30A; XPV"
                     /EC_number="2.7.7.7"
                     /note="isoform 1 is encoded by transcript variant 1;
                     DNA-directed DNA polymerase eta; RAD30 homolog A;
                     xeroderma pigmentosum variant type protein homolog"
                     /codon_start=1
                     /product="DNA polymerase eta isoform 1"
                     /protein_id="NP_109640.1"
                     /db_xref="CCDS:CCDS28822.1"
                     /db_xref="GeneID:80905"
                     /db_xref="MGI:MGI:1891457"
                     /translation="MAPGQNRVVALVDMDCFFVQVEQRQNPHLRNKPCAVVQYKSWKG
                     GGIIAVSYEARAFGVTRNMWADDAKKLCPDLLLAQVRESRGKANLTKYREASVEVMEI
                     MSYFAVIERASIDEAYIDLTSAVQERLQKLQGQPISADLLPSTYIEGLPRGPTVEETV
                     QKEAIRKQGLLQWLDSLQSDDPTSPDLRLTVGAMIVEEMRAAIESKTGFQCSAGISHN
                     KVLAKLACGLNKPNRQTLVSHGSVPQLFSQMPIRKIRSLGGKLGASVIEVLGIEYMGD
                     LTQFTESQLQSHFGEKNGSWLYAMCRGIEHDPVKPRQLPKTIGCSKNFPGKTALATRE
                     QVQWWLLQLALELEERLTKDRNDNDRVATQLVVSIRFQGDRRLSSLRRCCALPRYDAH
                     KMSQDAFAAIRNCNTSGIQTEWSPPLTMLFLCATKFSAAAPPACTDITAFLSSDSSCQ
                     PKVPIASSETRTQGSGPAVPTSKEAATSLASFFQKAAKKQRMKETSFVPLNTATEKLS
                     SKPSLVFQSSQTTGSQSFFKQKSLLLQHTQLSNSAAPDPPQASPAAQPSCLPAECVDS
                     GPDDGAVKPVSSKAVSTEMNVAGDSPNVLDSPAYNSQEVTQRATEDQVLCEKCDSLVP
                     VWDMPEHTDYHFALELQKSFLQPCTSKPQAIPAVSPQGKRNPKSPSASSSKRLRPHGM
                     QTLESFFKPLTH"
     exon            389..523
                     /gene="Polh"
                     /gene_synonym="RAD30A; XPV"
                     /inference="alignment:Splign:2.1.0"
     exon            524..738
                     /gene="Polh"
                     /gene_synonym="RAD30A; XPV"
                     /inference="alignment:Splign:2.1.0"
     exon            739..908
                     /gene="Polh"
                     /gene_synonym="RAD30A; XPV"
                     /inference="alignment:Splign:2.1.0"
     exon            909..1012
                     /gene="Polh"
                     /gene_synonym="RAD30A; XPV"
                     /inference="alignment:Splign:2.1.0"
     exon            1013..1132
                     /gene="Polh"
                     /gene_synonym="RAD30A; XPV"
                     /inference="alignment:Splign:2.1.0"
     exon            1133..1256
                     /gene="Polh"
                     /gene_synonym="RAD30A; XPV"
                     /inference="alignment:Splign:2.1.0"
     exon            1257..1322
                     /gene="Polh"
                     /gene_synonym="RAD30A; XPV"
                     /inference="alignment:Splign:2.1.0"
     exon            1323..1492
                     /gene="Polh"
                     /gene_synonym="RAD30A; XPV"
                     /inference="alignment:Splign:2.1.0"
     exon            1493..3234
                     /gene="Polh"
                     /gene_synonym="RAD30A; XPV"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3215..3220
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Polh"
                     /gene_synonym="RAD30A; XPV"
                     /note="hexamer: AATAAA"
     polyA_site      3234
                     /gene="Polh"
                     /gene_synonym="RAD30A; XPV"
                     /note="major polyA site"
ORIGIN      
        1 aaccgtccct cgcggcgcgc gcggtgctga actccaaatt tcctctgggc ttgctgtggg
       61 gcgtaaacct gcaggctgga agggctgttg gctcggcggg agcctagggc tacctactcg
      121 gatctgtgtt atcagattac actcacttgc tcctgggaag gcgacgacga catctagacg
      181 ctgtcattgg actccgcccg gagcccgcag cttcgctctt ggcagttttg acaggtgtcg
      241 ccttgaaata aatggctcct gggcagaatc gagtggttgc tcttgtagac atggactgct
      301 tttttgtaca agtggaacag cggcaaaatc ctcatttgag gaataaacct tgtgcagttg
      361 tacagtacaa atcatggaaa ggtggtggaa ttattgcagt tagctatgaa gcccgagcat
      421 ttggtgtcac tagaaacatg tgggcggatg atgctaagaa gttatgtcca gatcttctcc
      481 tggcacaagt tcgtgagtcc cgtggaaaag ctaacctcac caagtaccgg gaagctagtg
      541 ttgaagtgat ggagataatg tcttattttg ctgtgattga acgtgccagc atcgatgagg
      601 cttatataga tttgaccagt gctgtacaag aaagacttca aaagttacaa ggtcagccta
      661 tctcagcaga tttgctgcca agcacttaca ttgaagggtt gccccgaggc cctacagtag
      721 aagagactgt tcagaaagag gctattcgga aacaaggctt gcttcagtgg cttgattctc
      781 ttcagagtga tgatcccacc tctccagacc tcaggctgac tgtgggagcc atgattgtgg
      841 aagaaatgag agcagccata gagagtaaga ctggcttcca gtgttcagct ggaatctcac
      901 acaataaggt cctggcaaaa cttgcttgtg gactaaataa gcccaaccgc caaaccctgg
      961 tctcacatgg gtcagtgcca cagctcttca gccagatgcc aatccgtaaa atccgaagtc
     1021 ttggaggaaa gctaggggct tctgtcattg aagtccttgg gatagaatac atgggtgacc
     1081 tgacccagtt cactgaatcc cagctccaga gtcattttgg agagaagaat gggtcttggc
     1141 tgtatgccat gtgtcgaggt attgaacacg atccagttaa acccaggcag ttacctaaaa
     1201 ctattggctg cagcaagaac ttcccaggga aaacagctct ggctactcgg gagcaggtac
     1261 agtggtggct tttgcagtta gctctggaac ttgaggagag gctaaccaaa gatcgaaatg
     1321 ataatgaccg cgtggccact cagctggtcg tcagtattcg ttttcaggga gacaggcgtc
     1381 tcagcagcct gcggcggtgc tgtgccctcc ctcgctatga cgctcacaag atgagccagg
     1441 acgcatttgc tgccatcagg aactgtaata catctggaat ccaaactgag tggtcccctc
     1501 ctctcacaat gctcttcctc tgcgctacca agttctctgc ggctgcccct ccagcttgca
     1561 cggatatcac tgcgttcctg agtagcgact caagttgtca gccaaaggtg ccaattgcca
     1621 gctctgagac taggacccag gggtctggcc cagctgtacc aacctctaaa gaggcagcca
     1681 catctctagc gtcttttttt caaaaagctg ccaaaaagca gagaatgaaa gagacttcct
     1741 ttgtacctct taacacagcc acagagaagt tatcatccaa gccttcactt gtgttccaaa
     1801 gcagccaaac cacaggaagt cagtctttct ttaagcagaa gagcctgctg ctccagcaca
     1861 cacagcttag taactctgcg gctcctgacc ctccacaggc cagccctgca gcgcaaccaa
     1921 gctgtctgcc agcagagtgt gtagacagtg gtcctgatga tggagcggtg aagccagtat
     1981 cctctaaagc agtgtctaca gaaatgaatg tggctgggga cagtccaaac gtgctcgatt
     2041 ctccagctta caactctcag gaggtgaccc aaagggccac tgaggaccaa gtgctctgtg
     2101 agaagtgtga ctccctggtt ccagtgtggg acatgccaga acacacggac taccattttg
     2161 cattggagtt gcagaagtct tttttgcagc cttgtacttc aaaaccccag gctattcctg
     2221 ccgtgtctcc tcagggcaaa agaaatccca agagtccctc ggcttccagt agtaagcgcc
     2281 ttaggcccca tggcatgcag acgctggaat cgttttttaa gccactgaca cattagtgct
     2341 gcctggggca tcaggagggc tctactattt tttttctaac ggatggaatc atgttgactg
     2401 ctcaagggga atatgacatt tctaatacaa agttaggggt tttttcttcc aatgggactc
     2461 tgatcctgtt gctgatcaat gcttttcctc tctagggaga cagagtgtca taaaaggtat
     2521 aaagaacctg gcaagctcac ttgcttccct ataaactaac taaagactaa acaggagtat
     2581 aactcaaaaa aactgtaaaa gaaaggggaa atggggctgg agatagagct cacttgtcta
     2641 ggacggcatg tgcaaggccc catgttcact tcccagttct ggaaggaaaa cgaggactat
     2701 taagatgggc aagtgcaaac ggccaaagta atggatttag cagtcttttt cagccatgca
     2761 ggcttttctg ataaattaca tgactttgaa ttatgtatga aaccaggtag taggtttaat
     2821 ggataggaaa acagcataga tattttttcc tacataaata caaatcaact tttttttttt
     2881 tttttttttt tttttggttt ttcgagacag agtttctctg tgtagctctg gctgtcctgg
     2941 aactcacttt gtagaccagg ctggcctcga actcagaaat ccgcctgcct ctgctgggat
     3001 taaaggctca tcaacttact tataaattgg gtctttgaaa gatttgaaat tcaccgcgtt
     3061 tagccggaaa ttaccctttc ctttgacttt agtgtgtagc ttcttccctg gctagatatg
     3121 tagtttctta aaagcaagaa ctcttgcagt gacttacatg gaatatgttc ttgatgttgg
     3181 ttgaatgtat gtattaaatt agccacattg agacaataaa aatgtactct ttga
//
