LOCUS       NM_015810               1585 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus polymerase (DNA directed), gamma 2, accessory subunit
            (Polg2), transcript variant 1, mRNA.
ACCESSION   NM_015810
VERSION     NM_015810.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1585)
  AUTHORS   Gorvin,C.M., Ahmad,B.N., Stechman,M.J., Loh,N.Y., Hough,T.A.,
            Leo,P., Marshall,M., Sethi,S., Bentley,L., Piret,S.E., Reed,A.,
            Jeyabalan,J., Christie,P.T., Wells,S., Simon,M.M., Mallon,A.M.,
            Schulz,H., Huebner,N., Brown,M.A., Cox,R.D., Brown,S.D. and
            Thakker,R.V.
  TITLE     An N-Ethyl-N-Nitrosourea (ENU)-Induced Tyr265Stop Mutation of the
            DNA Polymerase Accessory Subunit Gamma 2 (Polg2) Is Associated With
            Renal Calcification in Mice
  JOURNAL   J Bone Miner Res 34 (3), 497-507 (2019)
   PUBMED   30395686
  REMARK    GeneRIF: studies have identified a mutant mouse model for inherited
            renal calcification associated with a Polg2 nonsense mutation.
REFERENCE   4  (bases 1 to 1585)
  AUTHORS   Jemt,E., Persson,O., Shi,Y., Mehmedovic,M., Uhler,J.P., Davila
            Lopez,M., Freyer,C., Gustafsson,C.M., Samuelsson,T. and
            Falkenberg,M.
  TITLE     Regulation of DNA replication at the end of the mitochondrial
            D-loop involves the helicase TWINKLE and a conserved sequence
            element
  JOURNAL   Nucleic Acids Res 43 (19), 9262-9275 (2015)
   PUBMED   26253742
REFERENCE   5  (bases 1 to 1585)
  AUTHORS   Humble,M.M., Young,M.J., Foley,J.F., Pandiri,A.R., Travlos,G.S. and
            Copeland,W.C.
  TITLE     Polg2 is essential for mammalian embryogenesis and is required for
            mtDNA maintenance
  JOURNAL   Hum Mol Genet 22 (5), 1017-1025 (2013)
   PUBMED   23197651
  REMARK    GeneRIF: Our data indicate that Polg2 function is critical to
            mammalian embryogenesis and mtDNA replication, and that a single
            copy of Polg2 is sufficient to sustain life.
REFERENCE   6  (bases 1 to 1585)
  AUTHORS   Thundathil,J., Filion,F. and Smith,L.C.
  TITLE     Molecular control of mitochondrial function in preimplantation
            mouse embryos
  JOURNAL   Mol Reprod Dev 71 (4), 405-413 (2005)
   PUBMED   15895466
REFERENCE   7  (bases 1 to 1585)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   8  (bases 1 to 1585)
  AUTHORS   Carrodeguas,J.A., Theis,K., Bogenhagen,D.F. and Kisker,C.
  TITLE     Crystal structure and deletion analysis show that the accessory
            subunit of mammalian DNA polymerase gamma, Pol gamma B, functions
            as a homodimer
  JOURNAL   Mol Cell 7 (1), 43-54 (2001)
   PUBMED   11172710
REFERENCE   9  (bases 1 to 1585)
  AUTHORS   Carrodeguas,J.A. and Bogenhagen,D.F.
  TITLE     Protein sequences conserved in prokaryotic aminoacyl-tRNA
            synthetases are important for the activity of the processivity
            factor of human mitochondrial DNA polymerase
  JOURNAL   Nucleic Acids Res 28 (5), 1237-1244 (2000)
   PUBMED   10666468
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL603664.16, AK041591.1 and BE853186.1.
            
            On Jun 17, 2009 this sequence version replaced NM_015810.1.
            
            Transcript Variant: This variant (1) represents the protein (1a)
            encoded by the C57BL/6 reference assembly.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK041591.1, BC119131.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164139, SAMN01164142
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-29                AL603664.16        135072-135100       c
            30-1464             AK041591.1         2-1436
            1465-1465           AL603664.16        123879-123879       c
            1466-1585           BE853186.1         1-120               c
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 70.01 cM"
     gene            1..1585
                     /gene="Polg2"
                     /gene_synonym="mtPolB; p55; polG-beta"
                     /note="polymerase (DNA directed), gamma 2, accessory
                     subunit"
                     /db_xref="GeneID:50776"
                     /db_xref="MGI:MGI:1354947"
     exon            1..579
                     /gene="Polg2"
                     /gene_synonym="mtPolB; p55; polG-beta"
                     /inference="alignment:Splign:2.1.0"
     CDS             96..1475
                     /gene="Polg2"
                     /gene_synonym="mtPolB; p55; polG-beta"
                     /EC_number="2.7.7.7"
                     /note="isoform 1a is encoded by transcript variant 1; DNA
                     polymerase subunit gamma-2, mitochondrial; DNA polymerase
                     gamma accessory 55 kDa subunit; mitochondrial DNA
                     polymerase accessory subunit; mitochondrial polymerase
                     gamma 2"
                     /codon_start=1
                     /product="DNA polymerase subunit gamma-2 isoform 1a"
                     /protein_id="NP_056625.2"
                     /db_xref="CCDS:CCDS25561.1"
                     /db_xref="GeneID:50776"
                     /db_xref="MGI:MGI:1354947"
                     /translation="MRCGGGARACRRACRCWLSGYAGPADGTQQPDAPEHAVAREALV
                     DLCRRRHFFSGTPQQLSTAALLSGCHARFGPLGVELRKNLASQWWSSMVVFREQVFAV
                     DSLHQEPGSSQPRDSAFRLVSPESIREILQDREPSKEQLVAFLENLLKTSGKLRATLL
                     HGALEHYVNCLDLVNRKLPFGLAQIGVCFHPVSNSNQTPSSVTRVGEKTEASLVWFTP
                     TRTSSQWLDFWLRHRLLWWRKFAMSPSNFSSADCQDELGRKGSKLYYSFPWGKEPIET
                     LWNLGDQELLHTYPGNVSTIQGRDGRKNVVPCVLSVSGDVDLGTLAYLYDSFQLAENS
                     FARKKSLQRKVLKLHPCLAPIKVALDVGKGPTVELRQVCQGLLNELLENGISVWPGYS
                     ETVHSSLEQLHSKYDEMSVLFSVLVTETTLENGLIQLRSRDTTMKEMMHISKLRDFLV
                     KYLASASNV"
     exon            580..706
                     /gene="Polg2"
                     /gene_synonym="mtPolB; p55; polG-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            707..812
                     /gene="Polg2"
                     /gene_synonym="mtPolB; p55; polG-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            813..986
                     /gene="Polg2"
                     /gene_synonym="mtPolB; p55; polG-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            987..1127
                     /gene="Polg2"
                     /gene_synonym="mtPolB; p55; polG-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            1128..1208
                     /gene="Polg2"
                     /gene_synonym="mtPolB; p55; polG-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            1209..1309
                     /gene="Polg2"
                     /gene_synonym="mtPolB; p55; polG-beta"
                     /inference="alignment:Splign:2.1.0"
     exon            1310..1577
                     /gene="Polg2"
                     /gene_synonym="mtPolB; p55; polG-beta"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1491..1496
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Polg2"
                     /gene_synonym="mtPolB; p55; polG-beta"
                     /note="hexamer: AATAAA"
     regulatory      1527..1532
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Polg2"
                     /gene_synonym="mtPolB; p55; polG-beta"
                     /note="hexamer: AATACA"
     polyA_site      1528
                     /gene="Polg2"
                     /gene_synonym="mtPolB; p55; polG-beta"
                     /note="major polyA site"
     regulatory      1551..1556
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Polg2"
                     /gene_synonym="mtPolB; p55; polG-beta"
                     /note="hexamer: TATAAA"
     polyA_site      1552
                     /gene="Polg2"
                     /gene_synonym="mtPolB; p55; polG-beta"
     polyA_site      1572
                     /gene="Polg2"
                     /gene_synonym="mtPolB; p55; polG-beta"
ORIGIN      
        1 atttccgctg tgcctgcacc ccaggccctg gtgctggtgt aggtttgaaa ctgcggctct
       61 gcacgggagg tgctggtgtt ccgactcctg aagcgatgcg ctgcggtggc ggcgccaggg
      121 cctgccgcag ggcctgcagg tgctggctgt ccgggtacgc gggtcccgca gatgggacgc
      181 agcagcctga cgcccccgag catgcggtgg cccgcgaggc gctggtagac ctctgccgga
      241 ggaggcactt tttcagcggg acgccgcagc agctcagcac ggctgctctg ctgagcgggt
      301 gccacgcgcg ctttggacct ttgggtgtag agttacgaaa gaacctagcc tcacagtggt
      361 ggtcctctat ggtggtgttc agggagcagg tcttcgcggt ggactccctc caccaggagc
      421 cgggctcttc gcagccgagg gacagtgcct tcaggttagt ctctccagaa agtatacgcg
      481 aaatcttgca agacagagag ccgagtaagg aacagctagt ggcatttctt gagaacttat
      541 taaaaacttc tgggaaacta cgggcgactc ttcttcacgg tgccttggag cactatgtta
      601 attgcctgga tctggtaaac agaaagctac ctttcggtct tgctcagatt ggagtctgtt
      661 tccatcctgt ttcgaacagc aatcagacac ccagcagtgt tacaagagtt ggtgaaaaga
      721 cggaagcttc cttggtatgg tttactccca caaggacttc gagccagtgg cttgatttct
      781 ggttgcgtca tcggcttctg tggtggagaa agtttgctat gagtccctct aacttcagca
      841 gcgctgattg tcaggatgaa ttgggacgca agggaagcaa actttactac agcttcccct
      901 ggggaaagga gccaatagaa accctgtgga acctaggaga tcaagaactc ctacacacat
      961 atcctggaaa tgtgtctacg atacagggtc gagatgggcg gaaaaacgtg gttccttgcg
     1021 ttctgtctgt aagtggggac gtagacctgg gcacgctggc ttacctctac gattccttcc
     1081 agctggcaga gaactccttt gccaggaaga agagcctcca gagaaaggtg cttaaacttc
     1141 acccttgttt agcccctatt aaggtcgctt tggatgtggg gaaaggccca acggtagaac
     1201 tccgacaggt ttgtcaaggg ctacttaatg agttactaga aaatgggatt tctgtgtggc
     1261 ctggctattc ggaaactgtc cattcctcat tagagcaact ccattcaaag tacgatgaga
     1321 tgagtgtcct cttctcagtt ctggtcactg agactacgct ggaaaatggg ctaatccagc
     1381 tgcggagcag agacaccacg atgaaggaaa tgatgcacat atccaagctg agagacttcc
     1441 tggtcaagta cctagcgtca gctagcaatg tgtaggtctt catattctct aataaatatt
     1501 ctctgttgag ttcattcgct tacattaata cagttttgga cttttgtatt tataaagtag
     1561 atatttgatt catttttaaa aaaaa
//
