LOCUS       NM_017462               7748 bp    mRNA    linear   ROD 16-OCT-2024
DEFINITION  Mus musculus polymerase (DNA directed), gamma (Polg), transcript
            variant 1, mRNA.
ACCESSION   NM_017462
VERSION     NM_017462.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7748)
  AUTHORS   Landoni,J.C., Erkul,S., Laalo,T., Goffart,S., Kivela,R., Skube,K.,
            Nieminen,A.I., Wickstrom,S.A., Stewart,J. and Suomalainen,A.
  TITLE     Overactive mitochondrial DNA replication disrupts perinatal cardiac
            maturation
  JOURNAL   Nat Commun 15 (1), 8066 (2024)
   PUBMED   39277581
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7748)
  AUTHORS   Kang,Y., Hepojoki,J., Maldonado,R.S., Mito,T., Terzioglu,M.,
            Manninen,T., Kant,R., Singh,S., Othman,A., Verma,R., Uusimaa,J.,
            Wartiovaara,K., Kareinen,L., Zamboni,N., Nyman,T.A., Paetau,A.,
            Kipar,A., Vapalahti,O. and Suomalainen,A.
  TITLE     Ancestral allele of DNA polymerase gamma modifies antiviral
            tolerance
  JOURNAL   Nature 628 (8009), 844-853 (2024)
   PUBMED   38570685
  REMARK    GeneRIF: Ancestral allele of DNA polymerase gamma modifies
            antiviral tolerance.
REFERENCE   3  (bases 1 to 7748)
  AUTHORS   Gonzalez,C.D., Nissanka,N., Van Booven,D., Griswold,A.J. and
            Moraes,C.T.
  TITLE     Absence of both MGME1 and POLG EXO abolishes mtDNA whereas absence
            of either creates unique mtDNA duplications
  JOURNAL   J Biol Chem 300 (4), 107128 (2024)
   PUBMED   38432635
  REMARK    GeneRIF: Absence of both MGME1 and POLG EXO abolishes mtDNA whereas
            absence of either creates unique mtDNA duplications.
REFERENCE   4  (bases 1 to 7748)
  AUTHORS   Ross,J.M., Olson,L. and Coppotelli,G.
  TITLE     Mitochondrial Dysfunction and Protein Homeostasis in Aging:
            Insights from a Premature-Aging Mouse Model
  JOURNAL   Biomolecules 14 (2), 162 (2024)
   PUBMED   38397399
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7748)
  AUTHORS   Yang,L., Ruan,Z., Lin,X., Wang,H., Xin,Y., Tang,H., Hu,Z., Zhou,Y.,
            Wu,Y., Wang,J., Qin,D., Lu,G., Loomes,K.M., Chan,W.Y. and Liu,X.
  TITLE     NAD+ dependent UPRmt activation underlies intestinal aging caused
            by mitochondrial DNA mutations
  JOURNAL   Nat Commun 15 (1), 546 (2024)
   PUBMED   38228611
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7748)
  AUTHORS   Da Cruz,S., Xenarios,I., Langridge,J., Vilbois,F., Parone,P.A. and
            Martinou,J.C.
  TITLE     Proteomic analysis of the mouse liver mitochondrial inner membrane
  JOURNAL   J Biol Chem 278 (42), 41566-41571 (2003)
   PUBMED   12865426
REFERENCE   7  (bases 1 to 7748)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   8  (bases 1 to 7748)
  AUTHORS   Zhang,D., Mott,J.L., Chang,S.W., Denniger,G., Feng,Z. and
            Zassenhaus,H.P.
  TITLE     Construction of transgenic mice with tissue-specific acceleration
            of mitochondrial DNA mutagenesis
  JOURNAL   Genomics 69 (2), 151-161 (2000)
   PUBMED   11031098
REFERENCE   9  (bases 1 to 7748)
  AUTHORS   Mott,J.L., Denniger,G., Zullo,S.J. and Zassenhaus,H.P.
  TITLE     Genomic structure of murine mitochondrial DNA polymerase-gamma
  JOURNAL   DNA Cell Biol 19 (10), 601-605 (2000)
   PUBMED   11058962
REFERENCE   10 (bases 1 to 7748)
  AUTHORS   Zullo,S.J., Butler,L., Zahorchak,R.J., Macville,M., Wilkes,C. and
            Merril,C.R.
  TITLE     Localization by fluorescence in situ hybridization (FISH) of human
            mitochondrial polymerase gamma (POLG) to human chromosome band
            15q24-->q26, and of mouse mitochondrial polymerase gamma (Polg) to
            mouse chromosome band 7E, with confirmation by direct sequence
            analysis of bacterial artificial chromosomes (BACs)
  JOURNAL   Cytogenet Cell Genet 78 (3-4), 281-284 (1997)
   PUBMED   9465903
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC110909.10 and AK133584.1.
            
            On Jan 26, 2018 this sequence version replaced NM_017462.2.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK133584.1, BC053024.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-75                AC110909.10        50978-51052
            76-2598             AK133584.1         2-2524
            2599-2599           AC110909.10        61691-61691
            2600-3383           AK133584.1         2526-3309
            3384-3384           AC110909.10        65126-65126
            3385-4596           AK133584.1         3311-4522
            4597-7748           AC110909.10        67869-71020
FEATURES             Location/Qualifiers
     source          1..7748
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 45.04 cM"
     gene            1..7748
                     /gene="Polg"
                     /gene_synonym="PolgA"
                     /note="polymerase (DNA directed), gamma"
                     /db_xref="GeneID:18975"
                     /db_xref="MGI:MGI:1196389"
     exon            1..204
                     /gene="Polg"
                     /gene_synonym="PolgA"
                     /inference="alignment:Splign:2.1.0"
     exon            205..972
                     /gene="Polg"
                     /gene_synonym="PolgA"
                     /inference="alignment:Splign:2.1.0"
     CDS             365..4018
                     /gene="Polg"
                     /gene_synonym="PolgA"
                     /EC_number="2.7.7.7"
                     /note="isoform 1 is encoded by transcript variant 1; DNA
                     polymerase subunit gamma-1; polG-alpha; mitochondrial DNA
                     polymerase catalytic subunit; polymerase, gamma;
                     mitochondrial polymerase gamma; Pol gamma; DNA polymerase
                     gamma subunit 1 (Mitochondrial DNA polymerase catalytic
                     subunit) (PolG-alpha); mitochondrial DNA polymerase gamma;
                     3'-5' exodeoxyribonuclease; 5'-deoxyribose-phosphate
                     lyase"
                     /codon_start=1
                     /product="DNA polymerase subunit gamma-1 isoform 1"
                     /protein_id="NP_059490.2"
                     /db_xref="CCDS:CCDS21382.1"
                     /db_xref="GeneID:18975"
                     /db_xref="MGI:MGI:1196389"
                     /translation="MSRLLWKKVAGAKVASGPVPATARWVSSSVLDPVPSDGRPPSQM
                     PSSENGQLRLNPLLIQMLSRGLHEQIFGCGGEMPDEAAVQRSVEHLQKHGLWGQPATP
                     LPDVELRLPRLFGGNLDQHFRLLAQKQSLPYLEAAASLLEAQLPPEPKSWAWAEGWTR
                     YGPEGEAEPVAIPEERALVFDVEVCLAEGTCPTLAVAISPSAWYSWCSRRLVEERYSW
                     TSQLSPADLIPLGGSTSASSSTKQDGQEQLVVGHNVSFDRAHIREQYLIQDSRMRFLD
                     TMSMHMAISGLSSFQRSLWMGAKQGKHKTQQSTKRGQKSPRKANGPAISSWDWMDISS
                     ANNLADVHNLYVGGPPLEKEPRELFVKGSMRDIRENFQDLMQYCARDVWATFEVFQQQ
                     LPLFLERCPHPVTLAGMLEMGVSYLPVNQNWERYLTEAQNTYEELQREMKKSLMDLAN
                     DACQLLSGERYKEDPWLWDLEWDLQEFKQKKAKKVKKPASASKLPIEGAGPFGDPMDQ
                     EDPGPPSEEEELQRSVTAHNRLQQLRSTTDLLPKRPQHLPGHPGWYRKLCPRLDDPAW
                     APGPSLLSLQMRVTPKLMALTWDGFPLHYSDSHGWGYLVPGRRDNLTEPPVSPTVESA
                     AVTCPYRAIESLYRKHCLEQGKQQLEPQEVDLAEEFLLTDSSAMWQTVEELGCLDVEA
                     EAKMENSGLSQPLVLPAACAPKSSQPTYHHGNGPYNDVNIPGCWFFKLPHKDGNNYNV
                     GSPFAKDFLPKMEDGTLQAGPGGASGPRALEINKMISFWRNAHKRISSQMVVWLPRSA
                     LPRVVTRHPSFDEEGHYGAILPQVVTAGTITRRAVEPTWLTASNARPDRVGSELKAMV
                     QAPPGYVLVGADVDSQELWIAAVLGDAHFAGMHGCTAFGWMTLQGRKSRGTDLHSKTA
                     ATVGISREHAKIFNYGRIYGAGQSFAERLLMQFNHRLTRQEAAEKAQQMYAVTKGLRR
                     YRLSADGEWLVKQLNLPVDRTEDGWVSLQDLRMIRREASRKSRWKKWEVASERAWTGG
                     TESEMFNKLESIAMSDTPRTPVLGCCISRALEPSVVQGEFITSRVNWVVQSSAVDYLH
                     LMLVAMKWLFEEFAIDGRFCISIHDEVRYLVREEDRYRAALALQITNLLTRCMFAYKL
                     GLNDLPQSVAFFSAVDIDQCLRKEVTMDCKTPSNPTGMERRYGIPQGEALDIYQIIEL
                     TKGSLEKRSQPGP"
     exon            973..1168
                     /gene="Polg"
                     /gene_synonym="PolgA"
                     /inference="alignment:Splign:2.1.0"
     exon            1169..1333
                     /gene="Polg"
                     /gene_synonym="PolgA"
                     /inference="alignment:Splign:2.1.0"
     exon            1334..1480
                     /gene="Polg"
                     /gene_synonym="PolgA"
                     /inference="alignment:Splign:2.1.0"
     exon            1481..1560
                     /gene="Polg"
                     /gene_synonym="PolgA"
                     /inference="alignment:Splign:2.1.0"
     exon            1561..1743
                     /gene="Polg"
                     /gene_synonym="PolgA"
                     /inference="alignment:Splign:2.1.0"
     exon            1744..1892
                     /gene="Polg"
                     /gene_synonym="PolgA"
                     /inference="alignment:Splign:2.1.0"
     exon            1893..2019
                     /gene="Polg"
                     /gene_synonym="PolgA"
                     /inference="alignment:Splign:2.1.0"
     exon            2020..2256
                     /gene="Polg"
                     /gene_synonym="PolgA"
                     /inference="alignment:Splign:2.1.0"
     exon            2257..2377
                     /gene="Polg"
                     /gene_synonym="PolgA"
                     /inference="alignment:Splign:2.1.0"
     exon            2378..2455
                     /gene="Polg"
                     /gene_synonym="PolgA"
                     /inference="alignment:Splign:2.1.0"
     exon            2456..2563
                     /gene="Polg"
                     /gene_synonym="PolgA"
                     /inference="alignment:Splign:2.1.0"
     exon            2564..2724
                     /gene="Polg"
                     /gene_synonym="PolgA"
                     /inference="alignment:Splign:2.1.0"
     exon            2725..2778
                     /gene="Polg"
                     /gene_synonym="PolgA"
                     /inference="alignment:Splign:2.1.0"
     exon            2779..2896
                     /gene="Polg"
                     /gene_synonym="PolgA"
                     /inference="alignment:Splign:2.1.0"
     exon            2897..3032
                     /gene="Polg"
                     /gene_synonym="PolgA"
                     /inference="alignment:Splign:2.1.0"
     exon            3033..3279
                     /gene="Polg"
                     /gene_synonym="PolgA"
                     /inference="alignment:Splign:2.1.0"
     exon            3280..3402
                     /gene="Polg"
                     /gene_synonym="PolgA"
                     /inference="alignment:Splign:2.1.0"
     exon            3403..3571
                     /gene="Polg"
                     /gene_synonym="PolgA"
                     /inference="alignment:Splign:2.1.0"
     exon            3572..3780
                     /gene="Polg"
                     /gene_synonym="PolgA"
                     /inference="alignment:Splign:2.1.0"
     exon            3781..3941
                     /gene="Polg"
                     /gene_synonym="PolgA"
                     /inference="alignment:Splign:2.1.0"
     exon            3942..7748
                     /gene="Polg"
                     /gene_synonym="PolgA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4389..4394
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Polg"
                     /gene_synonym="PolgA"
                     /note="hexamer: AATAAA"
     polyA_site      4407
                     /gene="Polg"
                     /gene_synonym="PolgA"
     regulatory      4574..4579
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Polg"
                     /gene_synonym="PolgA"
                     /note="hexamer: TATAAA"
     polyA_site      4597
                     /gene="Polg"
                     /gene_synonym="PolgA"
                     /note="major polyA site"
     regulatory      5046..5051
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Polg"
                     /gene_synonym="PolgA"
                     /note="hexamer: AATAAA"
     polyA_site      5069
                     /gene="Polg"
                     /gene_synonym="PolgA"
     regulatory      7728..7733
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Polg"
                     /gene_synonym="PolgA"
                     /note="hexamer: AATAAA"
     polyA_site      7748
                     /gene="Polg"
                     /gene_synonym="PolgA"
ORIGIN      
        1 attggcggcg ctcttattgg acgctcgggg gagtctcccg cctgcctctg ggtagccgcg
       61 ggacccagtt cgcgcagacg ggaagttgcg gctgctcccg agaccgcacg ccaccctgag
      121 gctgcgtggg ccgcgcgccg cggacgccgc gtcgtcgccg gggctgtggg ctgccgcaga
      181 acgggaagcg cgaagcggac cgaggacttg tgtgaggaag gcaggcatgg tgagacctat
      241 ttcactgaca ggagcacaga gaggggacgc gtctctctct gagtctttcg gccactaaaa
      301 gcagtcaagc tggagcccaa agcccggtgc cccgactcac agcggggggc tccctgcacc
      361 aaccatgagc cgcctgctct ggaagaaggt ggctggcgcc aaggtcgcct cagggccagt
      421 accagcaacc gcgcgctggg tctccagctc cgtcctcgac cccgtcccca gcgacgggcg
      481 gccgccgtcg caaatgccct cctccgagaa tgggcagctg cggctcaacc ctctgctcat
      541 ccagatgttg tcgagaggcc tgcacgagca gatcttcggg tgcggcgggg aaatgcccga
      601 cgaggccgcg gtgcagcgca gcgtagagca cctgcagaag cacgggctct gggggcagcc
      661 ggccacgccc ttgccagacg tggagctgcg cctgccccgg ctcttcgggg gcaacctgga
      721 ccagcacttc cgcctcctgg cccagaagca gagcctgcct tacttggagg cggccgcctc
      781 gttattggag gcccaattgc cccccgagcc caagagctgg gcctgggcgg agggctggac
      841 ccggtacggc cccgaggggg aggccgaacc cgtggccatc cccgaggagc gggccctggt
      901 gttcgacgtg gaggtctgct tggcagaggg aacctgcccc actttggcgg tggccatatc
      961 cccctcggcc tggtattcct ggtgcagccg gcggctggtg gaagagcgtt actcttggac
     1021 cagccagcta tcgccggctg acctaatccc tttggggggc tccactagtg ccagcagctc
     1081 caccaagcag gatgggcagg aacagttagt ggtggggcac aatgtttcct ttgaccgagc
     1141 ccatatcagg gaacagtatc tgattcagga ctcccggatg cgttttctcg atactatgag
     1201 catgcacatg gccatctcgg ggctgagcag cttccagcgc agcctgtgga tgggagccaa
     1261 gcagggaaag cacaagaccc agcagtccac aaagcgaggg cagaagtccc cgaggaaagc
     1321 caatggtcca gcgatctcat cttgggactg gatggatatc agcagtgcca ataatcttgc
     1381 agatgtgcac aacctttatg tggggggacc tcccttagag aaggagcctc gggagctgtt
     1441 cgtcaaaggc agcatgaggg atatccgaga gaacttccag gatctgatgc agtactgcgc
     1501 ccgtgatgtg tgggccacct ttgaggtttt ccagcagcag ctgccactct tcttggagag
     1561 gtgtccccac ccagtgactc tggctggcat gctggagatg ggtgtgtcct acctgcctgt
     1621 caaccagaac tgggagcgtt acctgacaga ggcacagaac acatatgagg agctccagcg
     1681 ggagatgaag aagtcgctga tggatctggc taatgatgcc tgtcagttgc tctcaggaga
     1741 gaggtacaaa gaagaccctt ggctctggga cctagaatgg gatttgcagg agtttaagca
     1801 gaaaaaggca aagaaggtga agaagccagc ctcagccagc aagttgccca tcgagggagc
     1861 tgggcccttt ggggatccca tggatcagga agatcctggc ccgcccagcg aggaggagga
     1921 gcttcagcga agtgtaacag cccacaaccg gttacagcag ctgaggagca ccacggacct
     1981 cctgcctaag cgaccccagc accttccagg acaccctggg tggtaccgga agctctgccc
     2041 tcgactagat gaccctgcct gggctcccgg ccccagcctc ctcagcctgc aaatgcgggt
     2101 cactcctaag ctcatggcac tgacctggga tggttttcct ctacactact cagactctca
     2161 tggttggggc tacctagtgc ctgggcgtcg ggataatctg accgagccgc cagtgagccc
     2221 cactgtagaa tccgctgcag taacctgccc ctacagagcc atcgagtcct tatacaggaa
     2281 gcactgcctt gaacagggga agcagcaatt ggagccccag gaggtagacc tggctgagga
     2341 gttcttactc actgacagta gtgccatgtg gcaaacggta gaagaactgg gctgcttaga
     2401 cgtagaggct gaggccaaga tggagaattc agggctgagc caacctctag ttctgcctgc
     2461 tgcttgtgcc cccaaatcca gccagcccac gtatcaccat ggcaacggac cttataatga
     2521 tgtgaatatc cctggttgct ggtttttcaa gctgcctcac aaggacggta acaactacaa
     2581 tgtgggcagt ccctttgcca aagatttcct gcccaagatg gaggatggca ccctgcaggc
     2641 tggcccagga ggtgccagtg ggcctcgtgc cctggaaata aataaaatga tttctttttg
     2701 gaggaatgct cataaacgta tcagttccca gatggtggta tggctcccca ggtcagctct
     2761 gccccgggtt gtgaccaggc accccagctt cgatgaggaa ggccactatg gggccatcct
     2821 accccaggtg gtgactgctg gcaccatcac ccgtcgagct gtggagccca cgtggctcac
     2881 tgccagtaat gctcggcctg accgggtagg cagtgagttg aaagccatgg tgcaggctcc
     2941 acctggctat gtccttgtgg gtgctgatgt ggactcacag gaactgtgga tcgcagctgt
     3001 acttggagat gctcactttg ctgggatgca tggctgcacg gcctttggct ggatgactct
     3061 gcagggcagg aagagcagag gcactgatct gcacagcaag acagctgcca ctgtgggcat
     3121 cagccgagag catgccaaaa tcttcaacta tggccgcatc tatggggctg ggcagtcctt
     3181 tgctgagcgc ctactgatgc agttcaacca caggctcaca aggcaggagg cagctgagaa
     3241 ggcccagcag atgtatgcag tcacaaaagg cctgcgccgg tatcggctgt ctgcggatgg
     3301 tgagtggctg gtgaaacagt tgaatcttcc tgtggacagg acagaggacg gctgggtctc
     3361 cctacaagat cttcgaatga tccgaagaga agcttcaagg aagtcacgat ggaagaagtg
     3421 ggaggtagcc tctgaacgag catggacagg gggcacagag tcagaaatgt ttaataagct
     3481 ggagagtatt gccatgtctg atacaccacg taccccagta ctgggctgct gcatcagcag
     3541 agccttggag ccctcagttg tccagggaga gtttataacc agtcgtgtga actgggtggt
     3601 acagagctct gctgtagact acttacatct catgcttgtg gccatgaagt ggctgtttga
     3661 ggaatttgcc attgatgggc gcttctgcat cagcatccac gacgaggttc gctacctggt
     3721 gcgtgaggag gaccgctacc gtgccgccct ggcactgcag atcaccaatc tcctgaccag
     3781 gtgcatgttt gcctataagc tgggtctgaa tgatctgccc cagtcagtcg cctttttcag
     3841 tgcagtagac attgaccagt gcctcaggaa ggaagtgacc atggactgta aaactccttc
     3901 taacccaact gggatggaaa ggagatatgg gatcccccag ggtgaagcac tggatattta
     3961 ccagataatt gaactcacca aaggctcctt ggaaaaacga agccagcctg gaccctagct
     4021 ctgtctggag gttctgtatt tgctcctgtg gagctttatt ggagcggtgc aggttcctaa
     4081 actcaggctt tcagatgtgc ttttttgcaa aaggcagcca tttttctgta gcaggacctg
     4141 cccaggaaag tcctcctaac ggaaggcaca attcagtgcg ttcagaacca ggacaccaac
     4201 atcagtgcag gctgtgtgac aaggggtact gttgggcaca aatgaagcag atgccccaaa
     4261 ggtcacatta actcaggcat ttcttcctcc tcttcttggc tggttctctg tcctgctgtg
     4321 tatgtgctga tgcagtgccc tagaagggga gagtagaaat tttgacaacc ttgttctaag
     4381 gtgatgggaa taaaaacaaa caccaaactc ctggaacagt tgtgctttcc cgtctgctgc
     4441 ccaggttgtg acttagaact ccgtcctgag tagatggacg agatgctggg agcccggagc
     4501 atcacagcta cagacggcct ggggcacagt gatgtacgga actccgcatc agccggctag
     4561 ccagattatt ttatataaat gtctaatttt gactgggtct tgtctactga tattatttct
     4621 agcttttaca aacttaaaat ggaacccaca ttgggctcag gtgatgaatg ggcctacctt
     4681 gagggagggt gtaaaagcca gccagaatgc tgtagaaaca ggaccccagg acaggggagt
     4741 ttgtctgcta cttttcatag aaatatatac caaaagcata gcagaagtag gcaccaacct
     4801 cttcgttttc gtcttcctca tcctctcgaa gaaccatgtc gaggacactg cctttgatct
     4861 tgaagtcccg agaagtactc agcttcatgt gctgcatcag gtttacctgg gacagcagaa
     4921 ggtcctttca gtgtgtactt ggaggaccct ttgcttttac cagatgacat tcaccttgac
     4981 cacctctagc tgtgctgcct gccacagagc ttgctagact ccttccagca gtaaatgaga
     5041 gtggaaataa agaacatttt taccttggac tttttagaca gttggataag aaatttttca
     5101 tactgttcaa tggcaaagac aaggttaggg attggcttgg tttcacgaag aactttggcc
     5161 taagagtaaa aagatataag acatttcagg ttcagtggct tttgaggttt ttatgtagca
     5221 tctctcaaga aggaacccac tttggcacat gctgtatttc ttttttctga tagtcaaatg
     5281 aatttacatt gctaagatta gtccctaaaa tgagtatact gtgtagtttc ccgagtcact
     5341 tagactgacc tcccactctt catgcctgga cttcagataa gtgccaccac ccccactttt
     5401 aacaatgaca tacactgtta acttatttgg ctggtcttct ggacattagc tttatttcct
     5461 ttaagaaacc acgtggtgag gaccatcctg tgcctaggcc tttgtatatc ctaaaaggaa
     5521 ttgttacagc cccatttcct aaatatagta actttacccc cttcctgcac tcgccaattc
     5581 ttagaccctt atggagcgcc atttccactc ttgctttttt aatgtccaac aagacccgtg
     5641 ggtctgagtg gccaggggga gcttaccatg gttgtgctca ctgcagcctt ctccttttct
     5701 gagcactttg gggcgtcact gctcttattc taaagaaaca gaaacattgt cttattcttc
     5761 aagatgctat atctgggaag cacaaaagtt gtgagatata tatctccatc ccttgaatgt
     5821 ctgcctcctt gggtatgtat attcttggaa gtaccatatc agtgtagcca ctaagctact
     5881 atacagatag gattaaggta gcaggaacag ctgactaggg ctatgcagag gtctgcagcc
     5941 agtcttagag gtaatcttat acacaatata gtcactacac ttcggaagtt ataaagggtc
     6001 acagagacac ttccttgagt tgtaactagt atgaatctct tagacaggaa gaactggtat
     6061 tggtgtacac ctttagtccc acagaagagg tagaggcagg tgggtctcct tgagttctag
     6121 gccagtcatg ctacaggacc atgacccagg acaggcagaa agctacactg taaaaaccct
     6181 gtctgaaata atagcaacaa aatactaaca gggcaaaaga aagccaagat ctaagaagag
     6241 atgggagaaa ccacccagtt tgagtgaaac aggaaacctt catcttaaaa ggcttccgtt
     6301 ttaactttcc agcagcggag taggggcaag gaagcttctg aaagcagaga gactgtactg
     6361 gtgaagctac acagaaggta cacaggttga ttcttgtgtt atgctcgaaa aggttttgga
     6421 ggtgctggta gaaaaagtcc taggaagcag gggtaaagag taaggtcctg tattaagaag
     6481 ggacttcacc tgaacacatg ggatgggacc catctccggt ttgtggctga ggtggcaccc
     6541 tgttgagtct ccaagtaaac ctgtgcactg ccaataactg ctgactaaaa caggaccaag
     6601 actcacagct gtcttgagtc tctacaggat gctttatgaa gtcagtgtga ccataaactc
     6661 atacaaaaat gataagctga gcgtggtgac acatgccctt tgtacagaga gcctcaagta
     6721 catagttcta ggctagccag agcaacaaag tgggccctct caaagggtgt gtgcattgag
     6781 tatgacagca tgcttatagt tccagaactt gacctgaggc aggtggatct caagttcaag
     6841 actagcctgc actacacaga aactgaaaag attcgaggga cccagacctc aggagtggag
     6901 ggtatctatc ctacagaaac aacagattga aagaatggag tccacatggg aatgtacgaa
     6961 gtttgaagtg tgagaggaca tgtctgcaag cgtttaagag agaaaaggac caggggtaat
     7021 tagcttgtcc agaatatcca gctgggtgtg gcagtatact tccttaatcc ccaggcagat
     7081 agaagccaat gtcaagttag cctgggccac accatgcatg caggcaaaca gtctctgagg
     7141 atctatacat gacatcattt ctaacaaacc cctgccctcc acccctgaag tgctgctgca
     7201 ctcacacctc cggcacctga ccctgcctcc tcccatgtgt cagatgctgt tacactaaaa
     7261 agaggcccaa tgcatggact tgcaggcacc cggaaatgag ccaaggctgc cttgttcctt
     7321 ctgtactcca aatagaagta taccaaatgc ctcctatctc tagaatagag ataggtttca
     7381 ctgttctgct ggctagtctt acagcaactt gacacacaag ccagagttat ctgaaaggag
     7441 tgaacctcgg ttgagaaaat gtctccataa gacccagctg tagggcattt tcttagttac
     7501 tggtatagtt actggtgaag ggccctgtct actgtggggg tgctacacct acactggtag
     7561 ttttgggtcc tataagaaag taggctgagt aagccacagg aagaaagcca gcagatcacc
     7621 tcacctccag agcctctgca tcagcttctg cctccaggtt cctgccctgt ttgagttcct
     7681 gtcctaactt cctccaatga aggactatga tctgggagtg taagccaaat aaaccctttt
     7741 ctcttcaa
//
