LOCUS       NM_025882               1694 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus polymerase (DNA-directed), epsilon 4 (p12 subunit)
            (Pole4), transcript variant 1, mRNA.
ACCESSION   NM_025882
VERSION     NM_025882.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Borel,V., Boeing,S., Van Wietmarschen,N., Sridharan,S., Hill,B.R.,
            Ombrato,L., Perez-Lloret,J., Jackson,D., Goldstone,R.,
            Boulton,S.J., Nussenzweig,A. and Bellelli,R.
  TITLE     Disrupted control of origin activation compromises genome integrity
            upon destabilization of Polepsilon and dysfunction of the
            TRP53-CDKN1A/P21 axis
  JOURNAL   Cell Rep 39 (9), 110871 (2022)
   PUBMED   35649380
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Bellelli,R., Youds,J., Borel,V., Svendsen,J.,
            Pavicic-Kaltenbrunner,V. and Boulton,S.J.
  TITLE     Synthetic Lethality between DNA Polymerase Epsilon and RTEL1 in
            Metazoan DNA Replication
  JOURNAL   Cell Rep 31 (8), 107675 (2020)
   PUBMED   32460026
REFERENCE   3  (bases 1 to 1694)
  AUTHORS   Bellelli,R., Belan,O., Pye,V.E., Clement,C., Maslen,S.L.,
            Skehel,J.M., Cherepanov,P., Almouzni,G. and Boulton,S.J.
  TITLE     POLE3-POLE4 Is a Histone H3-H4 Chaperone that Maintains Chromatin
            Integrity during DNA Replication
  JOURNAL   Mol Cell 72 (1), 112-126 (2018)
   PUBMED   30217558
REFERENCE   4  (bases 1 to 1694)
  AUTHORS   Bellelli,R., Borel,V., Logan,C., Svendsen,J., Cox,D.E., Nye,E.,
            Metcalfe,K., O'Connell,S.M., Stamp,G., Flynn,H.R., Snijders,A.P.,
            Lassailly,F., Jackson,A. and Boulton,S.J.
  TITLE     Polepsilon Instability Drives Replication Stress, Abnormal
            Development, and Tumorigenesis
  JOURNAL   Mol Cell 70 (4), 707-721 (2018)
   PUBMED   29754823
REFERENCE   5  (bases 1 to 1694)
  AUTHORS   Miller,J.A., Nathanson,J., Franjic,D., Shim,S., Dalley,R.A.,
            Shapouri,S., Smith,K.A., Sunkin,S.M., Bernard,A., Bennett,J.L.,
            Lee,C.K., Hawrylycz,M.J., Jones,A.R., Amaral,D.G., Sestan,N.,
            Gage,F.H. and Lein,E.S.
  TITLE     Conserved molecular signatures of neurogenesis in the hippocampal
            subgranular zone of rodents and primates
  JOURNAL   Development 140 (22), 4633-4644 (2013)
   PUBMED   24154525
REFERENCE   6  (bases 1 to 1694)
  AUTHORS   Tang,F., Barbacioru,C., Nordman,E., Bao,S., Lee,C., Wang,X.,
            Tuch,B.B., Heard,E., Lao,K. and Surani,M.A.
  TITLE     Deterministic and stochastic allele specific gene expression in
            single mouse blastomeres
  JOURNAL   PLoS One 6 (6), e21208 (2011)
   PUBMED   21731673
REFERENCE   7  (bases 1 to 1694)
  AUTHORS   Guo,G., Huss,M., Tong,G.Q., Wang,C., Li Sun,L., Clarke,N.D. and
            Robson,P.
  TITLE     Resolution of cell fate decisions revealed by single-cell gene
            expression analysis from zygote to blastocyst
  JOURNAL   Dev Cell 18 (4), 675-685 (2010)
   PUBMED   20412781
REFERENCE   8  (bases 1 to 1694)
  AUTHORS   Pavlinkova,G., Salbaum,J.M. and Kappen,C.
  TITLE     Maternal diabetes alters transcriptional programs in the developing
            embryo
  JOURNAL   BMC Genomics 10, 274 (2009)
   PUBMED   19538749
  REMARK    Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC159655.8.
            
            On Apr 29, 2007 this sequence version replaced NM_025882.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK010300.1, AK167631.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164138, SAMN02415129
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-242               AC159655.8         9578-9819
            243-327             AC159655.8         10276-10360
            328-369             AC159655.8         10965-11006
            370-1694            AC159655.8         14407-15731
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 35.94 cM"
     gene            1..1694
                     /gene="Pole4"
                     /gene_synonym="2400007P05Rik; 5830430F06Rik"
                     /note="polymerase (DNA-directed), epsilon 4 (p12 subunit)"
                     /db_xref="GeneID:66979"
                     /db_xref="MGI:MGI:1914229"
     exon            1..242
                     /gene="Pole4"
                     /gene_synonym="2400007P05Rik; 5830430F06Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             27..383
                     /gene="Pole4"
                     /gene_synonym="2400007P05Rik; 5830430F06Rik"
                     /EC_number="2.7.7.7"
                     /note="isoform 1 is encoded by transcript variant 1; DNA
                     polymerase epsilon subunit 4; DNA polymerase II subunit 4;
                     DNA polymerase epsilon subunit p12"
                     /codon_start=1
                     /product="DNA polymerase epsilon subunit 4 isoform 1"
                     /protein_id="NP_080158.1"
                     /db_xref="CCDS:CCDS39524.1"
                     /db_xref="GeneID:66979"
                     /db_xref="MGI:MGI:1914229"
                     /translation="MAAAAAAGSGTPREEEAPGGEAAASQAQAPTSAPGGVRLSRLPL
                     ARVKALVKADPDVTLAGQEAIFILARAAELFVETIAKDAYCCAQQGKRKTLQRRDLDN
                     AIEAVDEFAFLEGTLD"
     exon            243..327
                     /gene="Pole4"
                     /gene_synonym="2400007P05Rik; 5830430F06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            328..369
                     /gene="Pole4"
                     /gene_synonym="2400007P05Rik; 5830430F06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            370..1694
                     /gene="Pole4"
                     /gene_synonym="2400007P05Rik; 5830430F06Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      652..657
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pole4"
                     /gene_synonym="2400007P05Rik; 5830430F06Rik"
                     /note="hexamer: AATAAA"
     polyA_site      675
                     /gene="Pole4"
                     /gene_synonym="2400007P05Rik; 5830430F06Rik"
                     /note="major polyA site"
     regulatory      1101..1106
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pole4"
                     /gene_synonym="2400007P05Rik; 5830430F06Rik"
                     /note="hexamer: TATAAA"
     polyA_site      1125
                     /gene="Pole4"
                     /gene_synonym="2400007P05Rik; 5830430F06Rik"
                     /note="major polyA site"
     regulatory      1477..1482
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pole4"
                     /gene_synonym="2400007P05Rik; 5830430F06Rik"
                     /note="hexamer: AATAAA"
     polyA_site      1496
                     /gene="Pole4"
                     /gene_synonym="2400007P05Rik; 5830430F06Rik"
ORIGIN      
        1 ccgcgcgcca cacgtttcgg gaggggatgg cggcagcggc ggctgctggg agcgggacgc
       61 ccagagagga agaggctccc ggaggggagg cagcggcttc gcaggcccaa gccccgacga
      121 gtgcacccgg gggagttcgc ctctcgaggc tgcctctggc gagagtaaag gccttggtga
      181 aggcagaccc tgacgtaacg ctggcgggac aggaagccat ctttatcctg gcgcgtgccg
      241 cggaactgtt cgtggagact atcgcaaaag atgcctactg ctgtgctcaa caaggaaaga
      301 ggaaaactct ccagaggaga gatttggata atgcaataga agctgtggat gaattcgctt
      361 ttctagaagg cactttggat taattgtgga gctggatggt ctgagccttg gctgcatctg
      421 tcacctttcc cctctgtaag cctcagcttt ggagaaagtc tgcgcatgta cacaatcttc
      481 ttactctttt tacccaagat agaagaagca gagagctctt ggataacgtt ctccaccaag
      541 tcttccatta cttgagtctg tcttccaatt caagcctgga tgtagctgat gctgtttggg
      601 gcagagatga aaggactgtt ctgcatagct ggcttaatga gcacagacta gaataaaagg
      661 tctttgttca gcttctttcc tgtgttttat attgcctttg ctcttgccct gtgactggga
      721 acccatgtga tcaggcacag actttcctca tctttctacc aaccactcca agtcagtctt
      781 tttattaaat ttgttctttc tcagcatgtc attggaatgt ggatcgataa tatatgggat
      841 acctcttctc cttagaagtc aaatcgatgt ggtcccaaca gttttattca agtataattc
      901 atgtgtcata ttcaattcaa actgtaacat tcggtggttt ttaaaaaaat attcatagat
      961 atatgtagtg agattttgaa cattttcctg tgaatggatt cacatgagtc ttgtgtatgg
     1021 caaaatttag ataatgttat ttaaagttaa tctatgctat gatatgtatt tatacccttt
     1081 aagttgtgaa atatatgtga tataaaattt cccattttaa ctgcttttca gtgtgttgct
     1141 cagtgagatt gaatattttc agagtgttgc acaaccttca ccatcattgg cctccagaac
     1201 tttgtaacag tgttctcagc ctggaacatt gtgcacatgg aacagtatct tcctttcctt
     1261 ttctcatctc acgttaggct aaattaattt tgttgttgtt gttattttgt gaagtgtggg
     1321 cagtgcactt ttagtaccta gaatgtagtg cttcaggaaa gcagcagaaa gctgtggcta
     1381 gggcttccta cagtgttctg cagtgtagat ctcttaggat agagtctgca ggatcttctc
     1441 cctagaatga tacattcagt cctgtaatac gttaaaaata aactttcaga aaaatactac
     1501 atttgtagaa aagtgttttt aggtcttgct tactaagtcc acccaaaact gacatttcag
     1561 ggttaccaga aactgactga catttatggc ttattattta ttgagttcag tttttatttg
     1621 agttcagtat ttttttccat taatatcctt tgttttgaga tctaatcgag attattaaat
     1681 tgtttttgct tctt
//
