LOCUS       NM_011132               7167 bp    mRNA    linear   ROD 05-DEC-2024
DEFINITION  Mus musculus polymerase (DNA directed), epsilon (Pole), mRNA.
ACCESSION   NM_011132
VERSION     NM_011132.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7167)
  AUTHORS   Zhu,M., Kim,J., Deng,Q., Ricciuti,B., Alessi,J.V.,
            Eglenen-Polat,B., Bender,M.E., Huang,H.C., Kowash,R.R., Cuevas,I.,
            Bennett,Z.T., Gao,J., Minna,J.D., Castrillon,D.H., Awad,M.M., Xu,L.
            and Akbay,E.A.
  TITLE     Loss of p53 and mutational heterogeneity drives immune resistance
            in an autochthonous mouse lung cancer model with high tumor
            mutational burden
  JOURNAL   Cancer Cell 41 (10), 1731-1748 (2023)
   PUBMED   37774698
REFERENCE   2  (bases 1 to 7167)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7167)
  AUTHORS   Galati,M.A., Hodel,K.P., Gams,M.S., Sudhaman,S., Bridge,T.,
            Zahurancik,W.J., Ungerleider,N.A., Park,V.S., Ercan,A.B.,
            Joksimovic,L., Siddiqui,I., Siddaway,R., Edwards,M., de Borja,R.,
            Elshaer,D., Chung,J., Forster,V.J., Nunes,N.M., Aronson,M.,
            Wang,X., Ramdas,J., Seeley,A., Sarosiek,T., Dunn,G.P., Byrd,J.N.,
            Mordechai,O., Durno,C., Martin,A., Shlien,A., Bouffet,E., Suo,Z.,
            Jackson,J.G., Hawkins,C.E., Guidos,C.J., Pursell,Z.F. and Tabori,U.
  TITLE     Cancers from Novel Pole-Mutant Mouse Models Provide Insights into
            Polymerase-Mediated Hypermutagenesis and Immune Checkpoint Blockade
  JOURNAL   Cancer Res 80 (24), 5606-5618 (2020)
   PUBMED   32938641
REFERENCE   4  (bases 1 to 7167)
  AUTHORS   Li,H.D., Lu,C., Zhang,H., Hu,Q., Zhang,J., Cuevas,I.C., Sahoo,S.S.,
            Aguilar,M., Maurais,E.G., Zhang,S., Wang,X., Akbay,E.A., Li,G.M.,
            Li,B., Koduru,P., Ly,P., Fu,Y.X. and Castrillon,D.H.
  TITLE     A PoleP286R mouse model of endometrial cancer recapitulates high
            mutational burden and immunotherapy response
  JOURNAL   JCI Insight 5 (14), 138829 (2020)
   PUBMED   32699191
  REMARK    GeneRIF: A PoleP286R mouse model of endometrial cancer
            recapitulates high mutational burden and immunotherapy response.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7167)
  AUTHORS   Li,H.D., Cuevas,I., Zhang,M., Lu,C., Alam,M.M., Fu,Y.X., You,M.J.,
            Akbay,E.A., Zhang,H. and Castrillon,D.H.
  TITLE     Polymerase-mediated ultramutagenesis in mice produces diverse
            cancers with high mutational load
  JOURNAL   J Clin Invest 128 (9), 4179-4191 (2018)
   PUBMED   30124468
  REMARK    GeneRIF: We engineered a conditional PoleP286R allele. PoleP286R/+
            embryonic fibroblasts exhibited a striking mutator phenotype and
            immortalized more efficiently. PoleP286R/+ mice were born at
            Mendelian ratios but rapidly developed lethal cancers of diverse
            lineages, yielding the most cancer-prone monoallelic model
            described to date, to our knowledge.
REFERENCE   6  (bases 1 to 7167)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   7  (bases 1 to 7167)
  AUTHORS   Oshige,M., Yoshida,H., Hirose,F., Takata,K.I., Inoue,Y., Aoyagi,N.,
            Yamaguchi,M., Koiwai,O., Matsukage,A. and Sakaguchi,K.
  TITLE     Molecular cloning and expression during development of the
            Drosophila gene for the catalytic subunit of DNA polymerase epsilon
  JOURNAL   Gene 256 (1-2), 93-100 (2000)
   PUBMED   11054539
REFERENCE   8  (bases 1 to 7167)
  AUTHORS   Huang,D., Knuuti,R., Palosaari,H., Pospiech,H. and Syvaoja,J.E.
  TITLE     cDNA and structural organization of the gene Pole1 for the mouse
            DNA polymerase epsilon catalytic subunit
  JOURNAL   Biochim Biophys Acta 1445 (3), 363-371 (1999)
   PUBMED   10366722
REFERENCE   9  (bases 1 to 7167)
  AUTHORS   Goldsby,R.E., Singh,M. and Preston,B.D.
  TITLE     Mouse DNA polymerase epsilon gene (Pole) maps to chromosome 5
  JOURNAL   Mamm Genome 9 (1), 91-92 (1998)
   PUBMED   9434959
REFERENCE   10 (bases 1 to 7167)
  AUTHORS   Kamel,D., Mackey,Z.B., Sjoblom,T., Walter,C.A., McCarrey,J.R.,
            Uitto,L., Palosaari,H., Lahdetie,J., Tomkinson,A.E. and
            Syvaoja,J.E.
  TITLE     Role of deoxyribonucleic acid polymerase epsilon in spermatogenesis
            in mice
  JOURNAL   Biol Reprod 57 (6), 1367-1374 (1997)
   PUBMED   9408242
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ101052.1, AK141674.1 and BC048166.1.
            
            On Aug 7, 2008 this sequence version replaced NM_011132.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC048166.1, AF123502.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849383
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-40                CJ101052.1         5-44
            41-3889             AK141674.1         2-3850
            3890-7167           BC048166.1         3825-7102
FEATURES             Location/Qualifiers
     source          1..7167
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 53.45 cM"
     gene            1..7167
                     /gene="Pole"
                     /note="polymerase (DNA directed), epsilon"
                     /db_xref="GeneID:18973"
                     /db_xref="MGI:MGI:1196391"
     exon            1..131
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     CDS             70..6921
                     /gene="Pole"
                     /EC_number="2.7.7.7"
                     /note="DNA-directed DNA polymerase epsilon; DNA polymerase
                     II subunit A; pol-epsilon; 3'-5' exodeoxyribonuclease"
                     /codon_start=1
                     /product="DNA polymerase epsilon catalytic subunit A"
                     /protein_id="NP_035262.2"
                     /db_xref="CCDS:CCDS19525.1"
                     /db_xref="GeneID:18973"
                     /db_xref="MGI:MGI:1196391"
                     /translation="MVLRNSGRRHPEPGADGEGSRDDGPSSSVSALKRLERSQWTDKM
                     DLRFGFERLKEPGERTGWLINMHPTEILDEDKRLVSAVDYYFIQDDGSRFKVALPYMP
                     YFYIAARKGCDREVSSFLSKKFQGKIAKLENVPKEDLDLPNHLVGLKRSYIKLSFHTV
                     EDLVKVRKEISPAVKKNREQDHASDEYTTMLSSILQGGSVITDEDETSKKIADQLDNI
                     VDMREYDVPYHIRLSIDLRIHVAHWYNVRFRGNAFPVEITRRDDLVERPDPVVLAFDI
                     ETTKLPLKFPDAETDQIMMISYMIDGQGYLITNREIVSEDIEDFEFTPKPEYEGPFCV
                     FNEPDEVHLIQRWFEHIQETKPTIMVTYNGDFFDWPFVEARAAIHGLSMYQEIGFQKD
                     SQGEYKAPQCIHMDCLRWVKRDSYLPVGSHNLKAAAKAKLGYDPVELDPEDMCRMATE
                     QPQTLATYSVSDAVATYYLYMKYVHPFIFALCTIIPMEPDEVLRKGSGTLCEALLMVQ
                     AFHANIIFPNKQEQEFNKLTDDGHMLDAETYVGGHVEALESGVFRSDIPCRFRMNPAA
                     FDFLLQRVEKTMRHAIEEEEKVPVEQATNFQEVCEQIKTKLTSLKDVPNRIECPLIYH
                     LDVGAMYPNIILTNRLQPSAIVDEATCAACDFNKPGASCQRKMAWQWRGEFMPASRSE
                     YHRIQHQLESEKFPPLFPEGPARAFHELSREEQAKYEKRRLADYCRKAYKKIHVTKVE
                     ERLTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKLSAAVEVGDASEVKRCKNME
                     ILYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCFTGANIITQARELIEQIGRP
                     LELDTDGIWCVLPNSFPENFVIKTTNAKKPKLTISYPGAMLNIMVKEGFTNHQYQELT
                     EPSSLTYVTHSENSIFFEVDGPYLAMILPASKEEGKKLKKRYAVFNEDGSLAELKGFE
                     VKRRGELQLIKIFQSSVFEAFLKGSTLEEVYGSVAKVADYWLDVLYSKAANMPDSELF
                     ELISENRSMSRKLEDYGEQKSTSISTAKRLAEFLGDQMVKDAGLSCRYIISRKPEGSP
                     VTERAIPLAIFQAEPTVRKHFLRKWLKSSSLQDFDIRTILDWDYYIERLGSAIQKIIT
                     IPAALQQVKNPVPRVKHPDWLHKKLLEKNDIYKQKKISELFVLEGKRQIVMAQASENS
                     LSLCTPDMEDIGLTKPHHSTVPVATKRKRVWETQKESQDIALTVPWQEVLGQPPSLGT
                     TQEEWLVWLQFHKKKWQLQAQQRLALRKKQRLESAEDMPRLGPIREGPSTGLGSFLRR
                     TARSIMDLPWQIIQISETRQAGLFRLWAIIGNDLHCIKLSIPRVFYVNQRVAKAEDGP
                     AYRKVNRALPRSNIVYNLYEYSVPEDMYQEHINEINTELSVPDIEGVYETQVPLLFRA
                     LVQLGCVCVVNKQLTRHLSGWEAETFALEHLEMRSLAQFSYLEPGSIRHIYLYHHTQG
                     HKALFGVFIPSQRRASVFVLDTVRSNQMPGLSALYSSEHSLLLDKVDPKLLPPPKHTF
                     EVRAETNLKTICRAIQRFLLAYKEERRGPTLIAVQSSWELCRLTSEIPVLEEFPLVPI
                     RVADKISYAVLDWQRHGARRMIRHYLNLDLCLSQAFEMSRYFHIPVGNLPEDISTFGS
                     DLFFARHLQHHNHLLWLSPTSRPDLGGKEADDNRLVMEFDDRATVEINSSGCYSTVCV
                     ELDIQNLAVNTILQSHHVNDMEGAGSMGISFDVIQQASLEDMVTGNQAASALANYDET
                     ALCSSTFRILKSMVVGWVKEITQYHNIYADNQVMHFYRWLQSPCSLLHDPALHRTLHN
                     MMKKLFLQLIAEFKRLGSSVVYANFNRIILCTKKRRIEDALAYVEYITNSIHSKEIFH
                     SLTISFSRCWEFLLWMDPSNYGGIKGKVPSSIHCGQVKEQDSQAREETDEEEEDKEKD
                     EEEEGMGESEVEDLLENNWNILQFLPQAASCQSYFLMIVSAYIVAVYQSMKEELRHSA
                     PGSTPVKRKGASQFSQESEGATGSLPGMITFSQDYVANELTQSFFTITQKIQKKVTGS
                     RNTTEPSEMFPVLPGSHLLLNNPALEFIKYVCKVLSLDTNITNQVNKLNRDLLRLVDV
                     GEFSEEAQFRDPCHSYVLPEVICHSCNFCRDLDLCKDSSFSQDGAILPQWLCSNCQAP
                     YDSSAIESALVEALQRKLMAFTLQDLVCLKCRGMKETHMPVYCSCAGDFTLTIRTEVF
                     MEQIRIFQNIAKYYSMSYLQETIEWLLQTSPVSNC"
     exon            132..273
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            274..354
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            355..399
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            400..492
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            493..647
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            648..789
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            790..870
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            871..978
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            979..1089
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            1090..1175
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            1176..1295
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            1296..1428
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            1429..1542
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            1543..1755
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            1756..1863
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            1864..1992
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            1993..2095
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            2096..2242
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            2243..2388
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            2389..2537
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            2538..2630
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            2631..2775
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            2776..2933
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            2934..3129
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            3130..3344
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            3345..3447
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            3448..3528
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            3529..3651
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            3652..3861
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            3862..4071
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            4072..4215
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            4216..4356
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            4357..4510
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            4511..4617
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            4618..4794
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            4795..5018
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            5019..5239
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            5240..5444
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            5445..5618
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            5619..5744
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            5745..5886
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            5887..6064
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            6065..6196
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            6197..6390
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            6391..6591
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            6592..6717
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            6718..6807
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     exon            6808..7129
                     /gene="Pole"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7107..7112
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pole"
                     /note="hexamer: AGTAAA"
     polyA_site      7112
                     /gene="Pole"
                     /note="major polyA site"
     polyA_site      7129
                     /gene="Pole"
                     /note="major polyA site"
ORIGIN      
        1 gcctcgcgag agcacgtgga gagcgcgcca aattctcccc ggagcctgag ggagctttgg
       61 agcgtcgcaa tggtcctgag gaacagtgga cggaggcacc ccgagccggg cgcggatggc
      121 gaaggcagcc gggatgatgg tccctcttcc tcagtctcag cactcaagcg tctggaacgg
      181 agccagtgga cagacaagat ggacttacgg tttggtttcg aaaggctgaa agagcctgga
      241 gaaaggactg gctggctgat caacatgcac cctactgaga tcttagatga agacaaacgc
      301 ttagtcagcg cggtggatta ctacttcatt caagatgatg gaagcagatt taaggtggcc
      361 ttgccctata tgccgtattt ctacattgca gcgagaaagg gttgtgatcg agaagtttca
      421 tcttttctat ccaagaagtt tcagggaaaa attgcaaagt tagagaatgt gcccaaagaa
      481 gatctggact tgccaaatca cttggtgggc ttgaagcgga gttacatcaa gctgtccttc
      541 cacactgtgg aggaccttgt caaagtgaga aaggagatct ctcctgctgt gaagaagaac
      601 cgagagcagg accatgctag tgatgagtat acaacaatgc tctccagtat tctgcaaggt
      661 ggcagtgtaa ttactgatga ggacgaaacc tctaagaaga tagctgacca attggacaac
      721 atagtggaca tgcgagagta tgatgttccc taccacattc gcctctccat tgacctcaga
      781 atccatgtgg cccactggta caatgttaga tttcgaggaa atgcttttcc tgtggaaatc
      841 acccgacgag atgatcttgt tgaacgacct gaccctgtgg ttttggcatt tgacatcgag
      901 acgaccaaac tgcctctcaa attccctgat gctgagaccg atcagatcat gatgatctcc
      961 tatatgattg atggccaggg ctacctcatc actaacaggg agattgtttc agaagatatt
     1021 gaagattttg agttcacccc taagccagaa tatgaagggc ccttttgtgt cttcaatgaa
     1081 cccgacgagg tccatctgat ccagagatgg tttgagcata tccaggaaac caaacctacc
     1141 attatggtca cctacaatgg ggattttttt gactggccat ttgtggaggc tagggcagca
     1201 attcatggcc tcagcatgta ccaggagata ggcttccaga aggatagcca gggggaatat
     1261 aaggcaccac agtgcatcca catggactgc ctcaggtggg tgaagaggga cagttacctt
     1321 cctgtgggca gtcataatct caaggcagct gccaaggcca aacttggcta tgaccctgta
     1381 gagctggacc ctgaggacat gtgtcgtatg gccactgaac agccccagac tctggccact
     1441 tactcagtgt cagatgctgt ggctacttac tacctgtaca tgaaatacgt ccaccccttc
     1501 atattcgccc tgtgcaccat tattcccatg gaacctgatg aggtgctgcg gaagggctcc
     1561 gggacactgt gtgaagcctt gctgatggtg caagctttcc atgccaacat tatcttcccc
     1621 aataagcaag agcaggagtt caacaagctg acagatgatg gccacatgct agatgctgag
     1681 acctacgtcg ggggccacgt ggaggcacta gagtctggtg tcttcagaag tgatatcccc
     1741 tgccggttta ggatgaatcc tgcagccttt gatttcctgc tgcaacgagt cgagaagact
     1801 atgcgccacg ccattgaaga agaagagaag gtgcctgtgg aacaagccac caactttcaa
     1861 gaggtgtgtg agcagattaa gaccaagctc acctccctaa aagatgttcc taacagaatt
     1921 gaatgtcctc taatctacca tctagatgtg ggggccatgt atcctaacat aattcttacc
     1981 aaccgcctac agccttctgc catagtggat gaggccacct gtgctgcctg tgacttcaat
     2041 aagcctggag caagttgtca gaggaagatg gcctggcagt ggaggggaga attcatgcca
     2101 gccagtcgca gtgaatacca tcggattcag catcagctgg agtcggagaa gtttccccct
     2161 ttgtttccag aggggccagc acgggccttt cacgagctgt cccgtgaaga acaggctaaa
     2221 tacgagaaga ggaggctggc agattattgc cggaaagcct ataagaagat ccatgtgacc
     2281 aaggtagaag aacgtctaac taccatctgc cagcgggaaa actcatttta tgtggacaca
     2341 gtgcgggcct tcagagacag gcgctatgag ttcaaaggac tgcacaaggt gtggaagaag
     2401 aagctctcgg cagctgtaga ggtgggcgat gcatcagagg tgaagcgctg caagaacatg
     2461 gagatccttt acgattcact gcagctggct cacaagtgca tcctgaactc cttctacggc
     2521 tatgtcatgc gcaaaggagc tcgctggtat tccatggaga tggctggtat cgtctgcttt
     2581 acaggagcca acatcatcac ccaagcaaga gaactgattg agcagatcgg gaggccttta
     2641 gaattggaca cggacggaat atggtgcgtc ctacccaata gctttcctga aaattttgtc
     2701 atcaagacaa ccaatgcgaa gaaacccaaa ctgaccatct cctatcctgg tgccatgttg
     2761 aacatcatgg tcaaggaagg ctttaccaac caccagtacc aggaactaac agagccttcg
     2821 tctctcacct atgtcaccca ctctgagaat agtatctttt ttgaagtcga tggaccatac
     2881 cttgctatga tccttccagc ctccaaggaa gaaggcaaga agctgaagaa aagatatgct
     2941 gtgttcaatg aagatggttc cttggctgaa ctgaaaggtt ttgaggtgaa acgccgaggg
     3001 gagttgcagc tgattaaaat attccagtcc tcagtttttg aggccttcct caagggcagc
     3061 acactggagg aagtgtatgg ctcggtggcc aaagtggctg actactggct agatgtgctc
     3121 tatagcaagg ctgctaatat gcccgattct gaattgtttg agctgatttc tgagaaccgc
     3181 tccatgtctc ggaagctgga agattacggg gagcagaagt ctacatccat cagcacagca
     3241 aagcgcctgg ctgagttcct gggagaccag atggtcaaag atgctggact gagctgccgc
     3301 tatatcatct cccgaaagcc agaggggtct cctgtcactg agagggccat tccacttgcc
     3361 attttccaag cagagcctac agtgaggaaa cattttctcc ggaaatggct aaagagttca
     3421 tcacttcaag actttgatat tcggacaatt ctggactggg actactacat agagaggctg
     3481 gggagtgcca tccagaaaat catcaccatc cccgcagctc tgcagcaggt gaagaaccca
     3541 gttccacgtg tcaaacatcc agactggcta cacaaaaaac tactagagaa gaatgatatc
     3601 tacaaacaga agaagatcag tgagctcttt gtgcttgaag gaaagagaca gattgtgatg
     3661 gcccaggctt cagaaaacag tctgagtctc tgcactccag acatggagga cattggactc
     3721 acaaagccac accactctac agtcccagtt gctactaaga ggaagcgagt ctgggagacc
     3781 caaaaggagt ctcaggatat tgcactaact gtgccctggc aagaggtctt agggcagcct
     3841 ccctcccttg gaaccacaca ggaagagtgg ttggtctggc tccagttcca caagaaaaag
     3901 tggcagctgc aggcccaaca gcgcctagcc ctcaggaaga agcaacgctt agagtcagca
     3961 gaagatatgc caaggcttgg gcctatccga gaggggcctt ccacaggact ggggagcttt
     4021 ttgcgaagga ctgcccgcag catcatggac cttccatggc agataataca gatcagtgag
     4081 accagacagg ctggtctgtt ccggctgtgg gctatcattg gcaatgactt gcactgcatc
     4141 aagctgagta tccctcgagt attctatgta aaccagcggg ttgccaaagc agaggatgga
     4201 cctgcatatc ggaaggtgaa tcgggctctt cctcgttcca acattgtcta caatctctat
     4261 gagtattcag taccagagga catgtaccaa gaacacatca acgagatcaa cactgagttg
     4321 tcagtaccag acattgaggg cgtgtatgag acacaggtcc cattgttatt ccgggccctc
     4381 gtgcagctgg gctgtgtgtg tgtggtcaac aagcagctga caaggcacct ttcgggctgg
     4441 gaagctgaaa cttttgccct cgagcacctt gaaatgcgtt ctctggccca gttcagctac
     4501 ttggaaccag ggagtatccg ccatatctac ctgtaccatc acactcaggg ccacaaggca
     4561 ctctttgggg tctttatccc ctcacagcga agagcatctg tgtttgtgtt ggatactgta
     4621 cgaagcaacc aaatgccagg gctcagtgcc ctgtactcat cagaacacag cctgctgctg
     4681 gacaaggtgg accccaagct cctgcctccc ccaaaacaca cctttgaagt tcgtgctgaa
     4741 accaacctga agactatctg cagagccatc cagcgcttcc tgcttgccta caaggaagag
     4801 cgccgagggc ccacactcat cgctgtccag tctagctggg agctgtgtag gctgaccagt
     4861 gagattccag tcttagaaga gttcccacta gtgcctatcc gagtggctga caagatcagc
     4921 tatgcagtcc tggactggca gcgccatgga gctcgccgaa tgatccggca ctacctcaat
     4981 ttagacttgt gcctgtcgca ggcctttgag atgagcaggt acttccacat ccctgttgga
     5041 aacctgccgg aagacatctc cacctttggc tcagacctct tttttgcacg ccacctccag
     5101 caccataacc acctgctttg gctatcccct acctctcggc ctgacctggg tgggaaggaa
     5161 gctgatgaca accgccttgt catggagttt gatgaccgag ccactgtgga gatcaatagt
     5221 tctggctgtt actctactgt gtgcgtggaa ctggacattc aaaatctggc agtcaacacc
     5281 atcctccagt cccatcatgt caatgacatg gagggggctg gcagcatggg catcagcttc
     5341 gatgtgatcc agcaggcctc cctagaggac atggtaacag gcaatcaagc tgccagtgcc
     5401 ctggccaact acgatgagac agccctctgc tctagtacct tcaggatcct gaagagcatg
     5461 gtggttggct gggtaaagga aatcacacag taccacaaca tctatgctga caaccaggta
     5521 atgcacttct accgctggct ccagtcaccg tgctctctgc tccacgaccc agcccttcac
     5581 cggacgctgc acaacatgat gaagaagctc ttcctgcagc tcattgctga gttcaagcgc
     5641 ctggggtcat cagtcgtcta tgccaacttc aatcgcatca ttctctgtac aaagaagcgc
     5701 cgaatagagg atgcccttgc ctatgtggaa tatattacca acagcatcca ttctaaagag
     5761 atcttccatt ccctgaccat ctctttctct cgatgctggg aattccttct ctggatggat
     5821 ccatccaact atggtggaat caaaggaaaa gttccatcta gtattcactg tggacaggta
     5881 aaagagcaag actcccaggc aagagaggaa actgatgaag aggaggagga caaggaaaag
     5941 gacgaggagg aagagggcat gggagagtcc gaggttgagg acttactgga gaacaactgg
     6001 aacattctac agttcttgcc ccaggcagcc tcttgccaga gctacttcct catgattgtt
     6061 tcagcataca tcgtagctgt gtaccaaagc atgaaggagg agttgagaca cagtgccccg
     6121 ggcagtaccc ctgtgaagag gaagggggcc agccagttct cccaggagtc tgaaggggca
     6181 actggatctc ttcctggaat gatcactttc tctcaagatt atgtggcaaa tgagctcact
     6241 cagagcttct tcaccattac tcagaaaatt cagaagaaag tcacaggttc tcggaacacc
     6301 actgagccct cagagatgtt ccccgtcctc cctggttcac acttgctgct caataatcct
     6361 gctctggagt tcatcaaata tgtgtgcaag gtactatctc tggatacaaa catcacaaat
     6421 caggtgaata agctgaacag agaccttctt cgcctggtag acgttggtga attctctgag
     6481 gaggcccagt tcagagaccc ctgccactcc tacgtgctcc ctgaggtaat ctgccacagc
     6541 tgtaatttct gccgagacct ggacctgtgc aaagattcct ctttctctca ggatggagcc
     6601 atcctgcctc agtggctctg ctccaattgt caagccccct atgactcctc tgccattgag
     6661 tcagccttgg tggaagccct gcagaggaaa ctgatggcct tcacacttca ggacctggta
     6721 tgcctcaagt gccgtggtat gaaagagacc catatgcctg tgtactgcag ctgcgcaggg
     6781 gactttactc tcaccatccg cactgaggtc ttcatggaac agattagaat cttccagaac
     6841 attgccaagt actacagcat gtcatatctc caggagacca tagaatggct gttacagaca
     6901 agccctgtat caaactgtta gcaagcctag gctaaagaca ctttggtatc ccacacctac
     6961 tgcctgctcc aaaaggcaga accactgacc accttgcttt tccaaactca tgagcacagc
     7021 ccagaaggaa cagaagactt ctgctaacgt catcatgcca taaacagaca gaagcaggga
     7081 atggctctat ccctagctgc ctgctaagta aacacggttt tgaagcgtca aaaaaaaaaa
     7141 aaaaaaaaaa aaaaaaaaaa aaaaaaa
//
