LOCUS       NM_027196                939 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus polymerase (DNA-directed), delta 4 (Pold4), transcript
            variant 1, mRNA.
ACCESSION   NM_027196
VERSION     NM_027196.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 939)
  AUTHORS   Gu,X., Dai,Q., Du,P., Li,N., Li,J., Zeng,S., Peng,S., Tang,S.,
            Wang,L. and Zhou,Z.
  TITLE     Pold4 is dispensable for mouse development, DNA replication and DNA
            repair
  JOURNAL   Gene 851, 147029 (2023)
   PUBMED   36356905
  REMARK    GeneRIF: Pold4 is dispensable for mouse development, DNA
            replication and DNA repair.
REFERENCE   3  (bases 1 to 939)
  AUTHORS   Zhang,S., Zhou,Y., Sarkeshik,A., Yates,J.R. 3rd, Thomson,T.M.,
            Zhang,Z., Lee,E.Y. and Lee,M.Y.
  TITLE     Identification of RNF8 as a ubiquitin ligase involved in targeting
            the p12 subunit of DNA polymerase delta for degradation in response
            to DNA damage
  JOURNAL   J Biol Chem 288 (5), 2941-2950 (2013)
   PUBMED   23233665
  REMARK    GeneRIF: The identification of RNF8 allows new insights into the
            integration of the control of p12 degradation by different DNA
            damage signaling pathways.
            Erratum:[J Biol Chem. 2014 Jan 17;289(3):1212]
REFERENCE   4  (bases 1 to 939)
  AUTHORS   Dell'Era,P., Nicoli,S., Peri,G., Nieddu,M., Ennas,M.G. and
            Presta,M.
  TITLE     FGF2-induced upregulation of DNA polymerase-delta p12 subunit in
            endothelial cells
  JOURNAL   Oncogene 24 (6), 1117-1121 (2005)
   PUBMED   15608665
REFERENCE   5  (bases 1 to 939)
  AUTHORS   VanBuren,V., Piao,Y., Dudekula,D.B., Qian,Y., Carter,M.G.,
            Martin,P.R., Stagg,C.A., Bassey,U.C., Aiba,K., Hamatani,T.,
            Kargul,G.J., Luo,A.G., Kelso,J., Hide,W. and Ko,M.S.
  TITLE     Assembly, verification, and initial annotation of the NIA mouse
            7.4K cDNA clone set
  JOURNAL   Genome Res 12 (12), 1999-2003 (2002)
   PUBMED   12466305
REFERENCE   6  (bases 1 to 939)
  AUTHORS   Dell'Era,P., Coco,L., Ronca,R., Sennino,B. and Presta,M.
  TITLE     Gene expression profile in fibroblast growth factor 2-transformed
            endothelial cells
  JOURNAL   Oncogene 21 (15), 2433-2440 (2002)
   PUBMED   11948428
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC140073.4 and BC028520.1.
            
            On Jun 20, 2015 this sequence version replaced NM_027196.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the functional protein.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC028520.1, BY709901.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-85                AC140073.4         191407-191491       c
            86-939              BC028520.1         1-854
FEATURES             Location/Qualifiers
     source          1..939
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 3.93 cM"
     gene            1..939
                     /gene="Pold4"
                     /gene_synonym="2410012M21Rik; p12; Polds"
                     /note="polymerase (DNA-directed), delta 4"
                     /db_xref="GeneID:69745"
                     /db_xref="MGI:MGI:1916995"
     exon            1..296
                     /gene="Pold4"
                     /gene_synonym="2410012M21Rik; p12; Polds"
                     /inference="alignment:Splign:2.1.0"
     CDS             200..523
                     /gene="Pold4"
                     /gene_synonym="2410012M21Rik; p12; Polds"
                     /note="DNA polymerase delta smallest subunit p12"
                     /codon_start=1
                     /product="DNA polymerase delta subunit 4"
                     /protein_id="NP_081472.1"
                     /db_xref="CCDS:CCDS29424.1"
                     /db_xref="GeneID:69745"
                     /db_xref="MGI:MGI:1916995"
                     /translation="MGRKRFITDSYPVVKKREGPPGHSKGELAPELGEDTQSLSQEET
                     ELELLRQFDLAWQYGPCTGITRLQRWSRAEQMGLKPPLEVYQVLKAHPEDPHFQCSLW
                     HLYPL"
     exon            297..386
                     /gene="Pold4"
                     /gene_synonym="2410012M21Rik; p12; Polds"
                     /inference="alignment:Splign:2.1.0"
     exon            387..498
                     /gene="Pold4"
                     /gene_synonym="2410012M21Rik; p12; Polds"
                     /inference="alignment:Splign:2.1.0"
     exon            499..919
                     /gene="Pold4"
                     /gene_synonym="2410012M21Rik; p12; Polds"
                     /inference="alignment:Splign:2.1.0"
     regulatory      897..902
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pold4"
                     /gene_synonym="2410012M21Rik; p12; Polds"
                     /note="hexamer: AATAAA"
     polyA_site      917
                     /gene="Pold4"
                     /gene_synonym="2410012M21Rik; p12; Polds"
                     /note="major polyA site"
ORIGIN      
        1 gcgtgcttgt gggggcggaa gacaggcgtc attccctgga acctagggac ttcctctctc
       61 tgcttgtctg ggtcaacttg tctgtccatt gctggcccat ctctctctgg ccccctctct
      121 cactctcagc agctgtctct cacaggcccg ctgccctgcc ttttctttgc ctcctgctcc
      181 gcctgcctgg gtggccgcca tgggtcggaa gcggttcatc actgactcct atcctgttgt
      241 gaagaagagg gaggggcccc ctgggcacag caagggagag ctggcacccg agctagggga
      301 agacacccag tccctcagcc aggaggaaac agagctggag ctgctgaggc agtttgacct
      361 ggcctggcag tatgggcctt gtacaggtat cacaaggctg cagcgctgga gtcgggcaga
      421 gcagatgggc ttgaagcccc ccctagaggt gtaccaagtg ttgaaggcac accctgaaga
      481 ccctcacttc caatgcagcc tgtggcatct ctacccactc tgaggtaccc ccacgttgtc
      541 cttctctggg cacctcctag ccaccaagaa cttcaggaca gacatcatgg cctgctctgc
      601 tcattccaac cttggagaaa cagcaggctg actgtggagg tcttcctggt tcagcttctt
      661 ctccatctaa acagcaggct gctgaagctc ctgggacctg tgaagctgag cacctccacg
      721 ccctaatcat gaccaccaga tgcccacccg ccacacccct gacctacggt gtggcaaatc
      781 cagcacaggc tggcataaac cagatgtcgg cagataacct aaagggccca ccttgggcat
      841 ctagctgagg gcacagatga gtgagttatt ggatcaagga gctcactaat gcttacaata
      901 aagtacatta agcctgagaa aaaaaaaaaa aaaaaaaaa
//
