LOCUS       NM_008894               1634 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus polymerase (DNA directed), delta 2, regulatory subunit
            (Pold2), transcript variant 1, mRNA.
ACCESSION   NM_008894
VERSION     NM_008894.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Wu,X., Liu,Y., Wang,W., Crimmings,K., Williams,A., Mager,J. and
            Cui,W.
  TITLE     Early embryonic lethality of mice lacking POLD2
  JOURNAL   Mol Reprod Dev 90 (2), 98-108 (2023)
   PUBMED   36528861
  REMARK    GeneRIF: Early embryonic lethality of mice lacking POLD2.
REFERENCE   3  (bases 1 to 1634)
  AUTHORS   Bellelli,R., Borel,V., Logan,C., Svendsen,J., Cox,D.E., Nye,E.,
            Metcalfe,K., O'Connell,S.M., Stamp,G., Flynn,H.R., Snijders,A.P.,
            Lassailly,F., Jackson,A. and Boulton,S.J.
  TITLE     Polepsilon Instability Drives Replication Stress, Abnormal
            Development, and Tumorigenesis
  JOURNAL   Mol Cell 70 (4), 707-721 (2018)
   PUBMED   29754823
REFERENCE   4  (bases 1 to 1634)
  AUTHORS   Zhang,M., Chen,D., Xia,J., Han,W., Cui,X., Neuenkirchen,N.,
            Hermes,G., Sestan,N. and Lin,H.
  TITLE     Post-transcriptional regulation of mouse neurogenesis by Pumilio
            proteins
  JOURNAL   Genes Dev 31 (13), 1354-1369 (2017)
   PUBMED   28794184
REFERENCE   5  (bases 1 to 1634)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   6  (bases 1 to 1634)
  AUTHORS   Zheng,Y., Sheng,S., Wang,H., Jia,X., Hu,Y., Qian,Y., Zhu,Y. and
            Wang,J.
  TITLE     Identification of Pold2 as a novel interaction partner of protein
            inhibitor of activated STAT2
  JOURNAL   Int J Mol Med 30 (4), 884-888 (2012)
   PUBMED   22824807
  REMARK    GeneRIF: A direct interaction between Pold2 and PIAS2 was confirmed
            by direct yeast two-hybrid.
REFERENCE   7  (bases 1 to 1634)
  AUTHORS   Liu,L., Rodriguez-Belmonte,E.M., Mazloum,N., Xie,B. and Lee,M.Y.
  TITLE     Identification of a novel protein, PDIP38, that interacts with the
            p50 subunit of DNA polymerase delta and proliferating cell nuclear
            antigen
  JOURNAL   J Biol Chem 278 (12), 10041-10047 (2003)
   PUBMED   12522211
  REMARK    GeneRIF: The association of PDIP38 with pol delta was shown to
            occur in calf thymus tissue and mammalian cell extracts by
            GST-PDIP38 pull-down and coimmunoprecipitation experiments.
REFERENCE   8  (bases 1 to 1634)
  AUTHORS   Ducoux,M., Urbach,S., Baldacci,G., Hubscher,U., Koundrioukoff,S.,
            Christensen,J. and Hughes,P.
  TITLE     Mediation of proliferating cell nuclear antigen (PCNA)-dependent
            DNA replication through a conserved p21(Cip1)-like PCNA-binding
            motif present in the third subunit of human DNA polymerase delta
  JOURNAL   J Biol Chem 276 (52), 49258-49266 (2001)
   PUBMED   11595739
REFERENCE   9  (bases 1 to 1634)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   10 (bases 1 to 1634)
  AUTHORS   Hindges,R. and Hubscher,U.
  TITLE     Cloning, chromosomal localization, and interspecies interaction of
            mouse DNA polymerase delta small subunit (PolD2)
  JOURNAL   Genomics 44 (1), 45-51 (1997)
   PUBMED   9286699
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY093766.1, BG078511.2, CB205181.1, BM215242.2 and BY668936.1.
            
            On Apr 7, 2009 this sequence version replaced NM_008894.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: Z72486.1, BC003342.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-198               BY093766.1         2-199
            199-638             BG078511.2         152-591
            639-1269            CB205181.1         175-805
            1270-1601           BM215242.2         1-332               c
            1602-1634           BY668936.1         338-370
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 3.88 cM"
     gene            1..1634
                     /gene="Pold2"
                     /gene_synonym="50kDa; p50; po1D2"
                     /note="polymerase (DNA directed), delta 2, regulatory
                     subunit"
                     /db_xref="GeneID:18972"
                     /db_xref="MGI:MGI:1097163"
     exon            1..65
                     /gene="Pold2"
                     /gene_synonym="50kDa; p50; po1D2"
                     /inference="alignment:Splign:2.1.0"
     exon            66..342
                     /gene="Pold2"
                     /gene_synonym="50kDa; p50; po1D2"
                     /inference="alignment:Splign:2.1.0"
     CDS             123..1532
                     /gene="Pold2"
                     /gene_synonym="50kDa; p50; po1D2"
                     /EC_number="2.7.7.7"
                     /note="DNA polymerase delta subunit p50"
                     /codon_start=1
                     /product="DNA polymerase delta subunit 2"
                     /protein_id="NP_032920.2"
                     /db_xref="CCDS:CCDS24407.1"
                     /db_xref="GeneID:18972"
                     /db_xref="MGI:MGI:1097163"
                     /translation="MFSEQAAQRAHTLLAPPSASNATFARVPVATYTNSSQPFRLGER
                     SFNRQYAHIYATRLIQMRPFLVSRAQQHWGSRVEVKKLCELQPGEQCCVVGTLFKAMS
                     LQPSILREISEEHNLVPQPPRSKYIHPDDELVLEDELQRIKLKGTIDVSKLVTGTVLA
                     VLGSAKDDGRFQVEDHCFADLAPQKPVPPLDTDRFVLLVSGLGLGGGGGESLLGTQLL
                     VDVVTGQLGDEGEQCSAAHVSRVILAGNLLSHNTQSRDSINKAKYLTKKTQAASVEAV
                     KMLDEILLQLSASVPVDVMPGEFDPTNYTLPQQPLHPCMFPLATAYSTLQLVTNPYQA
                     TIDGVRFLGTSGQNVSDIFRYSSMEDHLEILEWTLRVRHISPTAPDTLGCYPFYKTDP
                     FIFPECPHVYFCGNTPSFGSKIIRGPEDQVVLLVAVPDFSSTQTACLVNLRSLACQPI
                     SFAGFGAEQEDLEGLGLGP"
     exon            343..464
                     /gene="Pold2"
                     /gene_synonym="50kDa; p50; po1D2"
                     /inference="alignment:Splign:2.1.0"
     exon            465..588
                     /gene="Pold2"
                     /gene_synonym="50kDa; p50; po1D2"
                     /inference="alignment:Splign:2.1.0"
     exon            589..703
                     /gene="Pold2"
                     /gene_synonym="50kDa; p50; po1D2"
                     /inference="alignment:Splign:2.1.0"
     exon            704..902
                     /gene="Pold2"
                     /gene_synonym="50kDa; p50; po1D2"
                     /inference="alignment:Splign:2.1.0"
     exon            903..983
                     /gene="Pold2"
                     /gene_synonym="50kDa; p50; po1D2"
                     /inference="alignment:Splign:2.1.0"
     exon            984..1141
                     /gene="Pold2"
                     /gene_synonym="50kDa; p50; po1D2"
                     /inference="alignment:Splign:2.1.0"
     exon            1142..1269
                     /gene="Pold2"
                     /gene_synonym="50kDa; p50; po1D2"
                     /inference="alignment:Splign:2.1.0"
     exon            1270..1371
                     /gene="Pold2"
                     /gene_synonym="50kDa; p50; po1D2"
                     /inference="alignment:Splign:2.1.0"
     exon            1372..1610
                     /gene="Pold2"
                     /gene_synonym="50kDa; p50; po1D2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1581..1586
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pold2"
                     /gene_synonym="50kDa; p50; po1D2"
                     /note="hexamer: AATAAA"
     polyA_site      1602
                     /gene="Pold2"
                     /gene_synonym="50kDa; p50; po1D2"
                     /note="major polyA site"
ORIGIN      
        1 gattggctgg atgcgccgat gggcgggacg ggcgcgcaga gattggcggg atgcggcgtg
       61 gcgcgtgata actcctcggc ctggacacag ctgcttggac caaacaagcc aggagtgccg
      121 ccatgttctc cgagcaggca gcacagaggg cccacactct gctcgccccg ccctctgcca
      181 gcaatgccac ctttgcccgg gtacctgtgg caacttacac caactcctca cagcccttcc
      241 gactgggaga gcgcagcttt aaccggcagt acgcccatat ttatgccacc cgcctcatcc
      301 agatgagacc gttcctggtg agccgggcgc agcaacactg gggcagccga gttgaagtga
      361 agaagttgtg tgagctgcag cctggggagc agtgctgtgt ggtgggcacg ctgttcaaag
      421 ccatgtccct ccaaccctcc atcctgcggg agatcagcga ggagcacaac ctggtccccc
      481 agcctccacg gagcaaatat atccacccgg atgacgagct ggtcttagaa gatgagctgc
      541 agcgtatcaa actgaaaggc accattgatg tgtcaaagct ggtcacagga acggtcttgg
      601 ccgtgttggg ctctgcgaaa gatgacggga ggtttcaggt tgaagaccac tgctttgctg
      661 acctggctcc acagaagccg gtacccccac ttgacacaga cagatttgtg ctactggtat
      721 ccggactggg cctgggcggt ggtggcgggg agagcctcct gggcacccag ctgctggtgg
      781 acgtggtgac gggacagctt ggggacgaag gagaacagtg cagtgctgcc cacgtctctc
      841 gagtcatcct cgcaggcaac ctgctcagcc ataacaccca gagcagagac tccatcaaca
      901 aggccaaata ccttaccaag aaaacccagg cagccagtgt ggaggcagtc aaaatgctgg
      961 acgagatcct tctgcaactg agtgcctcgg taccggtgga tgtgatgcca ggcgagtttg
     1021 atcccaccaa ctatacactc ccgcagcagc ccctgcaccc ctgcatgttc ccgctggcca
     1081 ccgcctactc cacactccag ctggtcacca acccatacca agccaccatt gatggagtaa
     1141 ggttcctggg gacatctgga cagaacgtga gtgatatctt ccggtatagc agcatggaag
     1201 accacttaga gatcctagag tggaccctgc gggttcgtca catcagcccc acggctccag
     1261 acaccctagg gtgctacccc ttctacaaaa ccgacccgtt catctttccg gaatgtcctc
     1321 acgtctactt ctgtggcaac acccccagct ttggttccaa aatcatccga ggtcctgagg
     1381 accaggtagt gctgttagtg gctgttcctg acttcagttc cacacagacg gcctgcctag
     1441 tgaacttacg cagcctggcc tgccagccta tcagctttgc gggcttcggg gcagagcaag
     1501 aggacctgga gggcctgggg ctgggtccct agtgagaggc agcgggctca ggacatcacc
     1561 cttgcaatgc aagccctgta aataaaacat gaatttttca caggtctgaa aaaaaaaaaa
     1621 aaaaaaaaaa aaaa
//
