LOCUS       NP_032920                469 aa            linear   ROD 27-APR-2025
DEFINITION  DNA polymerase delta subunit 2 [Mus musculus].
ACCESSION   NP_032920
VERSION     NP_032920.2
DBSOURCE    REFSEQ: accession NM_008894.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 469)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (residues 1 to 469)
  AUTHORS   Wu,X., Liu,Y., Wang,W., Crimmings,K., Williams,A., Mager,J. and
            Cui,W.
  TITLE     Early embryonic lethality of mice lacking POLD2
  JOURNAL   Mol Reprod Dev 90 (2), 98-108 (2023)
   PUBMED   36528861
  REMARK    GeneRIF: Early embryonic lethality of mice lacking POLD2.
REFERENCE   3  (residues 1 to 469)
  AUTHORS   Bellelli,R., Borel,V., Logan,C., Svendsen,J., Cox,D.E., Nye,E.,
            Metcalfe,K., O'Connell,S.M., Stamp,G., Flynn,H.R., Snijders,A.P.,
            Lassailly,F., Jackson,A. and Boulton,S.J.
  TITLE     Polepsilon Instability Drives Replication Stress, Abnormal
            Development, and Tumorigenesis
  JOURNAL   Mol Cell 70 (4), 707-721 (2018)
   PUBMED   29754823
REFERENCE   4  (residues 1 to 469)
  AUTHORS   Zhang,M., Chen,D., Xia,J., Han,W., Cui,X., Neuenkirchen,N.,
            Hermes,G., Sestan,N. and Lin,H.
  TITLE     Post-transcriptional regulation of mouse neurogenesis by Pumilio
            proteins
  JOURNAL   Genes Dev 31 (13), 1354-1369 (2017)
   PUBMED   28794184
REFERENCE   5  (residues 1 to 469)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   6  (residues 1 to 469)
  AUTHORS   Zheng,Y., Sheng,S., Wang,H., Jia,X., Hu,Y., Qian,Y., Zhu,Y. and
            Wang,J.
  TITLE     Identification of Pold2 as a novel interaction partner of protein
            inhibitor of activated STAT2
  JOURNAL   Int J Mol Med 30 (4), 884-888 (2012)
   PUBMED   22824807
  REMARK    GeneRIF: A direct interaction between Pold2 and PIAS2 was confirmed
            by direct yeast two-hybrid.
REFERENCE   7  (residues 1 to 469)
  AUTHORS   Liu,L., Rodriguez-Belmonte,E.M., Mazloum,N., Xie,B. and Lee,M.Y.
  TITLE     Identification of a novel protein, PDIP38, that interacts with the
            p50 subunit of DNA polymerase delta and proliferating cell nuclear
            antigen
  JOURNAL   J Biol Chem 278 (12), 10041-10047 (2003)
   PUBMED   12522211
  REMARK    GeneRIF: The association of PDIP38 with pol delta was shown to
            occur in calf thymus tissue and mammalian cell extracts by
            GST-PDIP38 pull-down and coimmunoprecipitation experiments.
REFERENCE   8  (residues 1 to 469)
  AUTHORS   Ducoux,M., Urbach,S., Baldacci,G., Hubscher,U., Koundrioukoff,S.,
            Christensen,J. and Hughes,P.
  TITLE     Mediation of proliferating cell nuclear antigen (PCNA)-dependent
            DNA replication through a conserved p21(Cip1)-like PCNA-binding
            motif present in the third subunit of human DNA polymerase delta
  JOURNAL   J Biol Chem 276 (52), 49258-49266 (2001)
   PUBMED   11595739
REFERENCE   9  (residues 1 to 469)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   10 (residues 1 to 469)
  AUTHORS   Hindges,R. and Hubscher,U.
  TITLE     Cloning, chromosomal localization, and interspecies interaction of
            mouse DNA polymerase delta small subunit (PolD2)
  JOURNAL   Genomics 44 (1), 45-51 (1997)
   PUBMED   9286699
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY093766.1, BG078511.2, CB205181.1, BM215242.2 and BY668936.1.
            
            On Apr 7, 2009 this sequence version replaced NP_032920.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: Z72486.1, BC003342.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 3.88 cM"
     Protein         1..469
                     /product="DNA polymerase delta subunit 2"
                     /EC_number="2.7.7.7"
                     /note="DNA polymerase delta subunit p50"
                     /calculated_mol_wt=51224
     Site            1
                     /site_type="acetylation"
                     /note="N-acetylmethionine.
                     /evidence=ECO:0000250|UniProtKB:P49005; propagated from
                     UniProtKB/Swiss-Prot (O35654.2)"
     Region          49..175
                     /region_name="DNA_pol_D_N"
                     /note="DNA polymerase delta subunit OB-fold domain;
                     pfam18018"
                     /db_xref="CDD:436215"
     Region          215..452
                     /region_name="MPP_PolD2_C"
                     /note="PolD2 (DNA polymerase delta, subunit 2), C-terminal
                     domain; cd07387"
                     /db_xref="CDD:277333"
     Site            order(216,220,222..227,277,281,284..285,288..289,448..452)
                     /site_type="other"
                     /note="PolD3 binding site [polypeptide binding]"
                     /db_xref="CDD:277333"
     Site            257
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P49005; propagated from
                     UniProtKB/Swiss-Prot (O35654.2)"
     CDS             1..469
                     /gene="Pold2"
                     /gene_synonym="50kDa; p50; po1D2"
                     /coded_by="NM_008894.2:123..1532"
                     /db_xref="CCDS:CCDS24407.1"
                     /db_xref="GeneID:18972"
                     /db_xref="MGI:MGI:1097163"
ORIGIN      
        1 mfseqaaqra htllappsas natfarvpva tytnssqpfr lgersfnrqy ahiyatrliq
       61 mrpflvsraq qhwgsrvevk klcelqpgeq ccvvgtlfka mslqpsilre iseehnlvpq
      121 pprskyihpd delvledelq riklkgtidv sklvtgtvla vlgsakddgr fqvedhcfad
      181 lapqkpvppl dtdrfvllvs glglggggge sllgtqllvd vvtgqlgdeg eqcsaahvsr
      241 vilagnllsh ntqsrdsink akyltkktqa asveavkmld eillqlsasv pvdvmpgefd
      301 ptnytlpqqp lhpcmfplat aystlqlvtn pyqatidgvr flgtsgqnvs difryssmed
      361 hleilewtlr vrhisptapd tlgcypfykt dpfifpecph vyfcgntpsf gskiirgped
      421 qvvllvavpd fsstqtaclv nlrslacqpi sfagfgaeqe dleglglgp
//
