LOCUS       NM_008893               2416 bp    mRNA    linear   ROD 12-MAY-2024
DEFINITION  Mus musculus polymerase (DNA directed), alpha 2 (Pola2), transcript
            variant 1, mRNA.
ACCESSION   NM_008893
VERSION     NM_008893.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Liu,T.Y., Chen,Y.C., Jong,Y.J., Tsai,H.J., Lee,C.C., Chang,Y.S.,
            Chang,J.G. and Chang,Y.F.
  TITLE     Muscle developmental defects in heterogeneous nuclear
            Ribonucleoprotein A1 knockout mice
  JOURNAL   Open Biol 7 (1) (2017)
   PUBMED   28077597
REFERENCE   3  (bases 1 to 2416)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   4  (bases 1 to 2416)
  AUTHORS   Gineste,C., De Winter,J.M., Kohl,C., Witt,C.C., Giannesini,B.,
            Brohm,K., Le Fur,Y., Gretz,N., Vilmen,C., Pecchi,E., Jubeau,M.,
            Cozzone,P.J., Stienen,G.J., Granzier,H., Labeit,S.,
            Ottenheijm,C.A., Bendahan,D. and Gondin,J.
  TITLE     In vivo and in vitro investigations of heterozygous nebulin
            knock-out mice disclose a mild skeletal muscle phenotype
  JOURNAL   Neuromuscul Disord 23 (4), 357-369 (2013)
   PUBMED   23375831
REFERENCE   5  (bases 1 to 2416)
  AUTHORS   Sehic,A., Risnes,S., Khan,Q.E., Khuu,C. and Osmundsen,H.
  TITLE     Gene expression and dental enamel structure in developing mouse
            incisor
  JOURNAL   Eur J Oral Sci 118 (2), 118-130 (2010)
   PUBMED   20487000
REFERENCE   6  (bases 1 to 2416)
  AUTHORS   Mizuno,T., Yamagishi,K., Miyazawa,H. and Hanaoka,F.
  TITLE     Molecular architecture of the mouse DNA polymerase alpha-primase
            complex
  JOURNAL   Mol Cell Biol 19 (11), 7886-7896 (1999)
   PUBMED   10523676
REFERENCE   7  (bases 1 to 2416)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
REFERENCE   8  (bases 1 to 2416)
  AUTHORS   Mizuno,T., Ito,N., Yokoi,M., Kobayashi,A., Tamai,K., Miyazawa,H.
            and Hanaoka,F.
  TITLE     The second-largest subunit of the mouse DNA polymerase
            alpha-primase complex facilitates both production and nuclear
            translocation of the catalytic subunit of DNA polymerase alpha
  JOURNAL   Mol Cell Biol 18 (6), 3552-3562 (1998)
   PUBMED   9584195
REFERENCE   9  (bases 1 to 2416)
  AUTHORS   Stadlbauer,F., Brueckner,A., Rehfuess,C., Eckerskorn,C.,
            Lottspeich,F., Forster,V., Tseng,B.Y. and Nasheuer,H.P.
  TITLE     DNA replication in vitro by recombinant
            DNA-polymerase-alpha-primase
  JOURNAL   Eur J Biochem 222 (3), 781-793 (1994)
   PUBMED   8026492
REFERENCE   10 (bases 1 to 2416)
  AUTHORS   Miyazawa,H., Izumi,M., Tada,S., Takada,R., Masutani,M., Ui,M. and
            Hanaoka,F.
  TITLE     Molecular cloning of the cDNAs for the four subunits of mouse DNA
            polymerase alpha-primase complex and their gene expression during
            cell proliferation and the cell cycle
  JOURNAL   J Biol Chem 268 (11), 8111-8122 (1993)
   PUBMED   8463324
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC131692.5.
            
            On Aug 20, 2022 this sequence version replaced NM_008893.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC021424.1, AK150099.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164135 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-376               AC131692.5         189636-190011
            377-501             AC131692.5         192622-192746
            502-593             AC131692.5         194713-194804
            594-651             AC131692.5         194962-195019
            652-758             AC131692.5         197723-197829
            759-952             AC131692.5         199953-200146
            953-1041            AC131692.5         200684-200772
            1042-1197           AC131692.5         202051-202206
            1198-1260           AC131692.5         202633-202695
            1261-1303           AC131692.5         203349-203391
            1304-1428           AC131692.5         205311-205435
            1429-1467           AC131692.5         206095-206133
            1468-1541           AC131692.5         207294-207367
            1542-1650           AC131692.5         210174-210282
            1651-1757           AC131692.5         210508-210614
            1758-1817           AC131692.5         211428-211487
            1818-1944           AC131692.5         211715-211841
            1945-2416           AC131692.5         212820-213291
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 4.34 cM"
     gene            1..2416
                     /gene="Pola2"
                     /note="polymerase (DNA directed), alpha 2"
                     /db_xref="GeneID:18969"
                     /db_xref="MGI:MGI:99690"
     exon            1..376
                     /gene="Pola2"
                     /inference="alignment:Splign:2.1.0"
     CDS             298..2100
                     /gene="Pola2"
                     /EC_number="2.7.7.7"
                     /note="isoform 1 is encoded by transcript variant 1; DNA
                     polymerase alpha subunit B; DNA polymerase alpha 70 kDa
                     subunit; DNA polymerase alpha 2, 68 kDa"
                     /codon_start=1
                     /product="DNA polymerase alpha subunit B isoform 1"
                     /protein_id="NP_032919.2"
                     /db_xref="CCDS:CCDS29485.1"
                     /db_xref="GeneID:18969"
                     /db_xref="MGI:MGI:99690"
                     /translation="MAVSTQQLAEELQIFGLDYEDSLLEKLAELCVLYRQTEDGMVSE
                     LIAFCTSAGKTCLTVDILNSFEYEVLNKKLSKAWHSASKDSGHAGTRDIVSIQELIEA
                     EEEEETLLSSYTTPSKGPLKRVSSTPETPLTKRSVAARSPRQLLSPSSFSPSATPSQK
                     YTSRTNRGEVVTTFGSAQGLSWSGRGGSGSVSLKVVGDPEPLTGSYKAMFQQLMGVRE
                     VLTSKIEELGSELKEHHKIEAFTPLLVPAQEPVILLGQIGCDSNGKLNSKSVILEGDQ
                     EHSYGAQIPVDLSELKEYSLFPGQVVIMEGFNTTGRRLTATKLYEGVPLPFYQPTEEE
                     GASEQTMVVVACGPYTTSDSITYDPLLDLIAIINRDQPDVCILFGPFLDAKHEQVENC
                     KLTSPFEDVFKQCLRTVIEGTRSSGSHLVFVPSLRDVHHEPVYPQPPFTFSELSREDK
                     KRVQFVSEPCSLSINGVMFGLTSTDLLFHIGAEEIFSSSGTSDRFSRVLKHILTQRSY
                     YPLYPPHEDMAIDYENFYTYAQLPVTPDVFIVPSELRYFVKDIFGCVCVNPGRLTKGQ
                     VGGTFGRLYLRRQPKAMDGGGRQGLSVAAQVVRI"
     exon            377..501
                     /gene="Pola2"
                     /inference="alignment:Splign:2.1.0"
     exon            502..593
                     /gene="Pola2"
                     /inference="alignment:Splign:2.1.0"
     exon            594..651
                     /gene="Pola2"
                     /inference="alignment:Splign:2.1.0"
     exon            652..758
                     /gene="Pola2"
                     /inference="alignment:Splign:2.1.0"
     exon            759..952
                     /gene="Pola2"
                     /inference="alignment:Splign:2.1.0"
     exon            953..1041
                     /gene="Pola2"
                     /inference="alignment:Splign:2.1.0"
     exon            1042..1197
                     /gene="Pola2"
                     /inference="alignment:Splign:2.1.0"
     exon            1198..1260
                     /gene="Pola2"
                     /inference="alignment:Splign:2.1.0"
     exon            1261..1303
                     /gene="Pola2"
                     /inference="alignment:Splign:2.1.0"
     exon            1304..1428
                     /gene="Pola2"
                     /inference="alignment:Splign:2.1.0"
     exon            1429..1467
                     /gene="Pola2"
                     /inference="alignment:Splign:2.1.0"
     exon            1468..1541
                     /gene="Pola2"
                     /inference="alignment:Splign:2.1.0"
     exon            1542..1650
                     /gene="Pola2"
                     /inference="alignment:Splign:2.1.0"
     exon            1651..1757
                     /gene="Pola2"
                     /inference="alignment:Splign:2.1.0"
     exon            1758..1817
                     /gene="Pola2"
                     /inference="alignment:Splign:2.1.0"
     exon            1818..1944
                     /gene="Pola2"
                     /inference="alignment:Splign:2.1.0"
     exon            1945..2416
                     /gene="Pola2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2395..2400
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pola2"
                     /note="hexamer: AATAAA"
     polyA_site      2416
                     /gene="Pola2"
                     /note="major polyA site"
ORIGIN      
        1 gaacctcgcg caagcgcgcg ctaggcaaag cgtttggcgg gtttttcctg tgctgtgctc
       61 ctcacggaga agctaaggtg tagtcctcgg aagcaagttt tctctctgga tcgagacctc
      121 tcgagggctt ttgaagtatt tctctgtgac ctggtggctg gggtcgtttg agggcttagg
      181 ccaggtggtc tcgtgacacg tccctgtcac cttccggtaa agcgaaagga taagcgtctg
      241 cagggtagag ctgaggtcgg ggcccggcgg caggcgcccg gccactcccg ggtcaccatg
      301 gccgtgtcca cccagcagct ggcggaggaa ctacagatct tcggcctgga ctacgaggac
      361 tccctgttgg agaaactggc agagctgtgt gttctatacc gacagactga ggacgggatg
      421 gtcagtgagc tcatcgcctt ctgcaccagt gcggggaaga catgccttac tgtagacatc
      481 ctgaactctt ttgaatacga ggttctgaac aaaaaactct ccaaagcctg gcacagtgcc
      541 tccaaagaca gtgggcatgc aggaaccaga gacattgttt ctatacaaga gctaattgaa
      601 gcagaggagg aagaggagac cctcctaagc tcttatacca caccctctaa gggtcctctc
      661 aagcgagtta gcagcacccc agaaaccccc ctaaccaaaa ggagcgtcgc tgctcgcagc
      721 ccacgtcagc ttctctcacc gtcaagtttc tccccgagtg ctaccccctc ccagaaatac
      781 acctccagaa ccaaccgggg agaagtggtg accacctttg gctccgcaca gggcttgtct
      841 tggtccggga gaggaggctc tggaagcgtc agtctgaagg tcgtggggga cccagagcca
      901 ctcactggca gctacaaagc gatgtttcag cagctcatgg gtgttcgaga agttctgacc
      961 agtaagatcg aagagctcgg cagcgagctg aaggagcatc acaagatcga ggcctttacc
     1021 cctctcctgg tcccagcgca agagcctgtt attctcctgg gtcagattgg ctgcgacagc
     1081 aacgggaagc tgaacagcaa gtccgtgatt ctcgaggggg accaggagca ctcctatggc
     1141 gcgcagatcc cagtggacct atcggaactc aaagagtatt ccctcttccc tggacaggtt
     1201 gtaattatgg aaggattcaa caccactggt aggagactta ctgccaccaa actctatgag
     1261 ggcgtgccac ttccgttcta tcagcctact gaagaggagg gagcttctga gcagactatg
     1321 gtggtagttg cctgcgggcc ttataccacc tccgacagca tcacctatga ccccctgctt
     1381 gatctgatcg ccatcatcaa ccgtgaccag ccagatgtct gcatcctgtt cggacctttc
     1441 ctggatgcta aacatgaaca ggtggagaac tgtaagttga caagcccatt cgaagacgtt
     1501 ttcaagcaat gcctgagaac agttatcgaa ggaacgagaa gctccggctc ccaccttgtc
     1561 tttgtcccgt ctctgaggga tgtccatcat gagccagtat acccacagcc gcctttcacc
     1621 ttctccgagc tgtctcgaga ggacaaaaag cgagtgcagt ttgtgtcaga gccctgcagc
     1681 ctctccataa atggagtgat gtttggtttg acatccacgg acctgctgtt ccacatcggg
     1741 gctgaggaga tctttagctc ttccgggacg tcagacagat tcagccgagt ccttaagcat
     1801 atcctgacgc agagaagtta ctacccactt tatccacccc acgaggacat ggccatagac
     1861 tacgagaact tctataccta tgcccagctg ccggtcaccc ccgatgtctt catagtgccg
     1921 tccgagctga gatactttgt gaaggacatc tttggctgtg tctgtgtgaa tcctgggcgg
     1981 ctcaccaaag gacaagtggg cggcaccttt ggccgcctct acctcaggag gcagcccaag
     2041 gccatggacg gtgggggcag gcagggcctg agcgtggcag cgcaggtggt cagaatctga
     2101 gtcctcagcc tggttgcctg gcctcacaaa caccaagagc ccaggtatgg ctctctggtg
     2161 gccaaactgt tggagtacct gacaaggatc tggccacagg gacaggactg tagggctggg
     2221 ccgtccagtc acggcttccc actgtccctc acccccatgc agcgtggctc tgatctgaat
     2281 tacgccctgc ttctctcctg cttcatgagg tagatgtaca gacgtgaacc atctatggac
     2341 acaaaggcag gaaaactgac tttttttgta tgaatcaaat acagcgtgtg gagaaataaa
     2401 aattttttta gtataa
//
