LOCUS       NM_008892               5350 bp    mRNA    linear   ROD 03-JUN-2024
DEFINITION  Mus musculus polymerase (DNA directed), alpha 1 (Pola1), mRNA.
ACCESSION   NM_008892
VERSION     NM_008892.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Mizuno,T., Hirabayashi,K., Miyazawa,S., Kobayashi,Y., Shoji,K.,
            Kobayashi,M., Hanaoka,F., Imamoto,N. and Torigoe,H.
  TITLE     The intrinsically disordered N-terminal region of mouse DNA
            polymerase alpha mediates its interaction with POT1a/b at telomeres
  JOURNAL   Genes Cells 26 (6), 360-380 (2021)
   PUBMED   33711210
  REMARK    GeneRIF: The intrinsically disordered N-terminal region of mouse
            DNA polymerase alpha mediates its interaction with POT1a/b at
            telomeres.
REFERENCE   2  (bases 1 to 5350)
  AUTHORS   Bellelli,R., Borel,V., Logan,C., Svendsen,J., Cox,D.E., Nye,E.,
            Metcalfe,K., O'Connell,S.M., Stamp,G., Flynn,H.R., Snijders,A.P.,
            Lassailly,F., Jackson,A. and Boulton,S.J.
  TITLE     Polepsilon Instability Drives Replication Stress, Abnormal
            Development, and Tumorigenesis
  JOURNAL   Mol Cell 70 (4), 707-721 (2018)
   PUBMED   29754823
REFERENCE   3  (bases 1 to 5350)
  AUTHORS   Ray,J.P., Staron,M.M., Shyer,J.A., Ho,P.C., Marshall,H.D.,
            Gray,S.M., Laidlaw,B.J., Araki,K., Ahmed,R., Kaech,S.M. and
            Craft,J.
  TITLE     The Interleukin-2-mTORc1 Kinase Axis Defines the Signaling,
            Differentiation, and Metabolism of T Helper 1 and Follicular B
            Helper T Cells
  JOURNAL   Immunity 43 (4), 690-702 (2015)
   PUBMED   26410627
REFERENCE   4  (bases 1 to 5350)
  AUTHORS   Cox,B.J., Vollmer,M., Tamplin,O., Lu,M., Biechele,S.,
            Gertsenstein,M., van Campenhout,C., Floss,T., Kuhn,R., Wurst,W.,
            Lickert,H. and Rossant,J.
  TITLE     Phenotypic annotation of the mouse X chromosome
  JOURNAL   Genome Res 20 (8), 1154-1164 (2010)
   PUBMED   20548051
REFERENCE   5  (bases 1 to 5350)
  AUTHORS   Eichinger,C.S., Mizuno,T., Mizuno,K., Miyake,Y., Yanagi,K.,
            Imamoto,N. and Hanaoka,F.
  TITLE     Aberrant DNA polymerase alpha is excluded from the nucleus by
            defective import and degradation in the nucleus
  JOURNAL   J Biol Chem 284 (44), 30604-30614 (2009)
   PUBMED   19726690
  REMARK    GeneRIF: two mechanisms that prevent the nuclear expression of
            aberrant DNA polymerase alpha p180
REFERENCE   6  (bases 1 to 5350)
  AUTHORS   Babalola,G.O. and Schultz,R.M.
  TITLE     Modulation of gene expression in the preimplantation mouse embryo
            by TGF-alpha and TGF-beta
  JOURNAL   Mol Reprod Dev 41 (2), 133-139 (1995)
   PUBMED   7654366
REFERENCE   7  (bases 1 to 5350)
  AUTHORS   Stadlbauer,F., Brueckner,A., Rehfuess,C., Eckerskorn,C.,
            Lottspeich,F., Forster,V., Tseng,B.Y. and Nasheuer,H.P.
  TITLE     DNA replication in vitro by recombinant
            DNA-polymerase-alpha-primase
  JOURNAL   Eur J Biochem 222 (3), 781-793 (1994)
   PUBMED   8026492
REFERENCE   8  (bases 1 to 5350)
  AUTHORS   Pendergrass,W.R., Saulewicz,A.C., Hanaoka,F. and Norwood,T.H.
  TITLE     Murine temperature-sensitive DNA polymerase alpha mutant displays a
            diminished capacity to stimulate DNA synthesis in senescent human
            fibroblast nuclei in heterokaryons at the nonpermissive condition
  JOURNAL   J Cell Physiol 158 (2), 270-276 (1994)
   PUBMED   8106564
REFERENCE   9  (bases 1 to 5350)
  AUTHORS   Linn,S.
  TITLE     How many pols does it take to replicate nuclear DNA?
  JOURNAL   Cell 66 (2), 185-187 (1991)
   PUBMED   1855251
  REMARK    Review article
REFERENCE   10 (bases 1 to 5350)
  AUTHORS   Adler,D.A., Tseng,B.Y., Wang,T.S. and Disteche,C.M.
  TITLE     Physical mapping of the genes for three components of the mouse DNA
            replication complex: polymerase alpha to the X chromosome, primase
            p49 subunit to chromosome 10, and primase p58 subunit to chromosome
            1
  JOURNAL   Genomics 9 (4), 642-646 (1991)
   PUBMED   2037291
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            D17384.1, BC138047.1 and CJ133126.1.
            
            On Oct 31, 2009 this sequence version replaced NM_008892.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D17384.1, D13543.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4493              D17384.1           1-4493
            4494-4591           BC138047.1         2357-2454
            4592-5320           D17384.1           4591-5319
            5321-5350           CJ133126.1         382-411
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 41.06 cM"
     gene            1..5350
                     /gene="Pola1"
                     /gene_synonym="Pola"
                     /note="polymerase (DNA directed), alpha 1"
                     /db_xref="GeneID:18968"
                     /db_xref="MGI:MGI:99660"
     exon            1..87
                     /gene="Pola1"
                     /gene_synonym="Pola"
                     /inference="alignment:Splign:2.1.0"
     exon            88..212
                     /gene="Pola1"
                     /gene_synonym="Pola"
                     /inference="alignment:Splign:2.1.0"
     exon            213..309
                     /gene="Pola1"
                     /gene_synonym="Pola"
                     /inference="alignment:Splign:2.1.0"
     exon            310..390
                     /gene="Pola1"
                     /gene_synonym="Pola"
                     /inference="alignment:Splign:2.1.0"
     exon            391..506
                     /gene="Pola1"
                     /gene_synonym="Pola"
                     /inference="alignment:Splign:2.1.0"
     exon            507..569
                     /gene="Pola1"
                     /gene_synonym="Pola"
                     /inference="alignment:Splign:2.1.0"
     exon            570..662
                     /gene="Pola1"
                     /gene_synonym="Pola"
                     /inference="alignment:Splign:2.1.0"
     exon            663..750
                     /gene="Pola1"
                     /gene_synonym="Pola"
                     /inference="alignment:Splign:2.1.0"
     exon            751..952
                     /gene="Pola1"
                     /gene_synonym="Pola"
                     /inference="alignment:Splign:2.1.0"
     exon            953..1125
                     /gene="Pola1"
                     /gene_synonym="Pola"
                     /inference="alignment:Splign:2.1.0"
     exon            1126..1238
                     /gene="Pola1"
                     /gene_synonym="Pola"
                     /inference="alignment:Splign:2.1.0"
     exon            1239..1355
                     /gene="Pola1"
                     /gene_synonym="Pola"
                     /inference="alignment:Splign:2.1.0"
     exon            1356..1430
                     /gene="Pola1"
                     /gene_synonym="Pola"
                     /inference="alignment:Splign:2.1.0"
     exon            1431..1569
                     /gene="Pola1"
                     /gene_synonym="Pola"
                     /inference="alignment:Splign:2.1.0"
     exon            1570..1724
                     /gene="Pola1"
                     /gene_synonym="Pola"
                     /inference="alignment:Splign:2.1.0"
     exon            1725..1809
                     /gene="Pola1"
                     /gene_synonym="Pola"
                     /inference="alignment:Splign:2.1.0"
     exon            1810..1871
                     /gene="Pola1"
                     /gene_synonym="Pola"
                     /inference="alignment:Splign:2.1.0"
     exon            1872..1961
                     /gene="Pola1"
                     /gene_synonym="Pola"
                     /inference="alignment:Splign:2.1.0"
     exon            1962..2078
                     /gene="Pola1"
                     /gene_synonym="Pola"
                     /inference="alignment:Splign:2.1.0"
     exon            2079..2254
                     /gene="Pola1"
                     /gene_synonym="Pola"
                     /inference="alignment:Splign:2.1.0"
     exon            2255..2384
                     /gene="Pola1"
                     /gene_synonym="Pola"
                     /inference="alignment:Splign:2.1.0"
     exon            2385..2504
                     /gene="Pola1"
                     /gene_synonym="Pola"
                     /inference="alignment:Splign:2.1.0"
     exon            2505..2604
                     /gene="Pola1"
                     /gene_synonym="Pola"
                     /inference="alignment:Splign:2.1.0"
     exon            2605..2729
                     /gene="Pola1"
                     /gene_synonym="Pola"
                     /inference="alignment:Splign:2.1.0"
     exon            2730..2879
                     /gene="Pola1"
                     /gene_synonym="Pola"
                     /inference="alignment:Splign:2.1.0"
     exon            2880..3002
                     /gene="Pola1"
                     /gene_synonym="Pola"
                     /inference="alignment:Splign:2.1.0"
     exon            3003..3035
                     /gene="Pola1"
                     /gene_synonym="Pola"
                     /inference="alignment:Splign:2.1.0"
     exon            3036..3128
                     /gene="Pola1"
                     /gene_synonym="Pola"
                     /inference="alignment:Splign:2.1.0"
     exon            3129..3334
                     /gene="Pola1"
                     /gene_synonym="Pola"
                     /inference="alignment:Splign:2.1.0"
     exon            3335..3467
                     /gene="Pola1"
                     /gene_synonym="Pola"
                     /inference="alignment:Splign:2.1.0"
     exon            3468..3599
                     /gene="Pola1"
                     /gene_synonym="Pola"
                     /inference="alignment:Splign:2.1.0"
     exon            3600..3774
                     /gene="Pola1"
                     /gene_synonym="Pola"
                     /inference="alignment:Splign:2.1.0"
     exon            3775..3953
                     /gene="Pola1"
                     /gene_synonym="Pola"
                     /inference="alignment:Splign:2.1.0"
     exon            3954..4085
                     /gene="Pola1"
                     /gene_synonym="Pola"
                     /inference="alignment:Splign:2.1.0"
     exon            4086..4202
                     /gene="Pola1"
                     /gene_synonym="Pola"
                     /inference="alignment:Splign:2.1.0"
     exon            4203..4296
                     /gene="Pola1"
                     /gene_synonym="Pola"
                     /inference="alignment:Splign:2.1.0"
     exon            4297..5347
                     /gene="Pola1"
                     /gene_synonym="Pola"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5323..5328
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pola1"
                     /gene_synonym="Pola"
                     /note="hexamer: AATAAA"
     polyA_site      5347
                     /gene="Pola1"
                     /gene_synonym="Pola"
                     /note="major polyA site"
ORIGIN      
        1 ccagtttcag gctggttggc gcggaataga gagactatcg gaccatggcg cccatgcacg
       61 aagaggactg taaactggag gcaagcgctg tgtcagattc agggagtttt gcagcttccc
      121 gagctcggcg agaaaagaaa tcaaagaaag gacgtcaaga agctttagag agactgaaaa
      181 aggctaaagc tggggagaag tataaatacg aagttgagga cctcacaagt gtttatgaag
      241 aagttgatga agagcagtat tcgaaactgg ttcaggcccg tcaggatgat gactggattg
      301 tggatgacga tgggataggc tatgtggaag atggcagaga gatttttgat gatgaccttg
      361 aggatgatgc acttgacacc tgtggaaaag gaagtgatgg caaagcacac agaaaggaca
      421 ggaaggatgt aaagaaacct tcagtgacaa aaccaaacaa tatcaaggca atgttcattg
      481 ctagtgccgg gaagaaaact acagataaag cagtagactt gtccaaggat gatctgctag
      541 gtgacattct acaggatcta aacactgaga cagctcaaat aactccacca cctgtattga
      601 taccaaagaa aaaaagatct actggggctt tgctcaaccc tttttcagtg cacaccccta
      661 aggcaattcc ttcaggaaaa cctgcttccc ctgtcctcag gaatgagcct ctgttaactc
      721 ccattcctct taaacgtgct gaacttgctg gagagctggc tcagccagaa tgtccagaag
      781 atgagcagga gctaggggta atggagtttg aagacggtga ctttgatgag tccatggaca
      841 cagagaaggt agatgagaag cctgtgactg ctaagacttg ggaccaagag actgagccag
      901 tagaaagggt ggaacatgag gcagatcctg agaggggaac cacatcttac ttagaaaatt
      961 ttctgccaga tgtctcttgt tgggacattg atcaggatga tgaaagcata cctcaggaag
     1021 ttcaagtgga ttccagtaat ctcccattgg taaaaggggc agatgatgaa caagtgtttc
     1081 agttttattg gctggatgct tatgaagatc catacaacca accaggtgtg gtttttctgt
     1141 ttggcaaagt gtggattgaa tcagtcaaaa cccatgtgag ctgttgtgtt atggtgaaaa
     1201 atattgagcg aacactctac ttccttcccc gtgaaatgaa atttgaccta aacacaggga
     1261 aagaaacagc aattccagtt acaatgaagg atgtttatga agaatttgat tcaaaaattt
     1321 cagcaaaata taaaataatg aagttcaagt ccaagatagt ggaaaagaac tatgcttttg
     1381 agatacctga tgttccagaa aaatctgagt acctggaagt tagatactcg gcagaagtgc
     1441 cacagcttcc acagaattta aaaggagaaa cattttctca tgtgtttgga actaacacgt
     1501 ctagcttgga actgttcttg atgaatagga agatcaaagg accttgttgg cttgaagtaa
     1561 aaaatccaca actcttgaat cagccaatca gttggtgtaa atttgaggta atggctttga
     1621 aaccagacct ggtgaatgta attaaggatg tcagtcctcc tccacttgtg gtgatgtctt
     1681 tcagcatgaa gacaatgcag aatgtacaga accatcaaca tgagattatt gctatggcgg
     1741 ctttggttca tcacagtttt gcattagata aagcacctcc agagcctccc tttcagacac
     1801 atttctgtgt tgtgtctaaa ccaaaggact gtattttccc atgtgatttc aaagaagtga
     1861 tttcgaaaaa gaatatgaag gttgagatcg ctgcaacaga aagaaccttg ataggatttt
     1921 tccttgcaaa agttcacaaa attgaccctg acattcttgt gggtcataat atttgtagtt
     1981 ttgaactgga agtgctgctg caaagaatta atgagtgcaa ggttccttac tggtccaaga
     2041 taggtcggct gaggagatcc aacatgccaa aacttgggag ccggagtgga tttggtgaaa
     2101 gaaatgctac ctgtggccga atgatatgtg atgtggaaat ttcagcaaaa gaattgattc
     2161 attgtaaaag ctaccatttg tctgaacttg ttcagcagat tctgaaaact gagaggattg
     2221 taattccaac agaaaatata cgaaacatgt atagtgaatc ttcttatctg ttgtacctgt
     2281 tggaacatat ctggaaagat gccagattca ttttgcagat catgtgtgag ctaaatgttc
     2341 ttccattagc attgcagatc actaacatcg ctgggaacat tatgtctagg acactgatgg
     2401 gtggacgatc tgagcgaaat gagtttttgt tgcttcatgc attttatgaa aacaactata
     2461 ttgtgcctga caagcagatt ttcagaaaac ctcagcaaaa actgggagat gaagatgagg
     2521 aaattgatgg ggacaccaat aaatacaaga aagggcgtaa gaaagcaact tacgctggag
     2581 gcttggtttt ggaccccaaa gttggttttt atgacaagtt cattttgctc ctggatttca
     2641 acagtttata tccttccatc attcaggaat ttaacatttg ttttacaaca gtgcaaagag
     2701 ttacttcaga ggtgcagaaa gctacagagg atgaggaaca agaacagatc cctgagttgc
     2761 cagatccaaa cttagagatg ggcattttgc ccagagagat tcggaaactg gtagagcgga
     2821 gaaaacaagt caaacagcta atgaaacagc aagatttaaa tccagacctt gttcttcagt
     2881 atgacatccg acagaaggct ttgaagctca cagcaaacag tatgtatggt tgcctgggat
     2941 tttcgtacag cagattttat gctaaaccac tggctgcctt ggtgacatac aaaggaaggg
     3001 agattttgat gcatacgaaa gacatggtac agaagatgaa tcttgaagtt atttatggag
     3061 acacagattc aattatgata aataccaata gcaccaacct ggaagaagta tttaagttgg
     3121 gaaacaaggt aaaaagtgaa gtgaataaat tgtacaaact gcttgaaata gacattgatg
     3181 ctgttttcaa gtctctgctg ttgctgaaga aaaagaaata tgctgctcta gttgttgaac
     3241 caacatcaga tgggaattac attaccaagc aggagctgaa aggattagat atagttagaa
     3301 gagactggtg tgatcttgcc aaggacactg gaaactttgt cattggccag attctttctg
     3361 atcaaagccg ggacaccatt gtggaaaaca ttcagaagag gttaatagaa attggagaaa
     3421 atgtgctaaa tggcagtgtc ccagtgagcc agtttgaaat taacaaggca ttgacaaagg
     3481 atccccagga ttaccctgat agaaaaagct tacctcatgt acatgttgca ctgtggataa
     3541 attctcaagg aggcaggaag gtgaaagctg gtgatacggt gtcctatgtt atttgtcagg
     3601 atgggtcaaa cctcactgca actcagaggg cctatgcccc agaacaattg cagaaacttg
     3661 acaatttagc catcgacacc caatactact tggcccagca gatccaccct gttgtggctc
     3721 ggatctgtga gccaatagat ggaattgatg ctgtactcat tgcattatgg ctaggacttg
     3781 actccactca atttagagtt catcagtatc acaaagatga agagaatgat gccctccttg
     3841 gtggtccagc acagctaact gatgaagaga aatacaagga ttgtgaaaaa ttcaaatgcc
     3901 tttgtccttc atgtggaact gaaaatattt atgataatgt ctttgaaggc tcgggactgg
     3961 atatggaacc cagtttgtat cgttgcagta atgttgattg taaggtttca ccgttgacct
     4021 tcatggtaca gctgagcaac aaattgatca tggacattag acggtgcatt aaaaagtact
     4081 atgatggctg gttgatatgt gaagagccaa cttgttgcag tcgccttcga cgtcttcccc
     4141 ttcatttctc ccgaaatggg cctctgtgtc cagtctgcat gaaagctgtg cttagaccag
     4201 agtattctga caagtccttg tacacccagc tgtgctttta ccggtacatt tttgatgcgg
     4261 actgtgcact ggaaaaactt actgaacatg agaaagataa attgaagaag cagtttttcc
     4321 ctctccgagt tcttcaggac tacagaaaag tcaagaacat agcagagcag ttcttgtcct
     4381 ggagtggcta ctctgaagtg aacctcagca agctcttcgc caattacgct gggaagtcct
     4441 gagagagccg ggaaggatgc aaggaggcca gctgcccaat ctgagctgct gaggcctccg
     4501 cctgccctaa ctgctgcacc actatcttgc ttttcttttg aggactgcat actgtgttga
     4561 tgttgatgta gatgtggacc agggtgggag tatcaaaata cttaacttac caaatagttt
     4621 ttatagcact atgtacacca aaccctttcc taagtatcta aagacctata tcttactgca
     4681 gggcagaaat tgctacttta attgagaata cctaagatgt aagtgacctt cagtttaccc
     4741 tggggcttta tcagagacac ccctaaaaag agttttatgg ggtctaatgg aagttctaac
     4801 attttgcttt ttcacatgtc agttaaaccc agtctgtgtc taaatacttg ttcagctact
     4861 ggctacaagc atggctgatt tcagtgctgt ctttcatata agtggtgcag gtggtaggga
     4921 ggtggtggtg gagggattta gaaagtttgc acttactaaa tacaaaaagc aagtttgctt
     4981 attgaattgt tcaaagttca acattcacca gatgtttcac tcacattcct tctattttcc
     5041 ctttacacag aatgttcaaa gtgtaggttt atacagtagg aaagcacatt ttaaatactt
     5101 tgtcctaaca tttcacaaaa tttatggtga ggttgtagta aggatcactg ttccattcag
     5161 aagaatggga cacagctgac aggatccagt gcttgctaga tttcatcagt tcttcagaat
     5221 agtgtctctt tgaagtgata aacaatatta caatatggca gccttaaata cttatctatt
     5281 tgtcttttta taaagaaaaa tgtattgtga tctgtaccta agaataaagt ttcctaaagg
     5341 aaaaccaaaa
//
