LOCUS       NM_172380               2835 bp    mRNA    linear   ROD 11-DEC-2024
DEFINITION  Mus musculus protein O-glucosyltransferase 1 (Poglut1), transcript
            variant 1, mRNA.
ACCESSION   NM_172380
VERSION     NM_172380.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Niknejad,N., Fox,D., Burwinkel,J.L., Zarrin-Khameh,N., Cho,S.,
            Soriano,A., Cast,A.E., Lopez,M.F., Huppert,K.A., Rigo,F.,
            Huppert,S.S., Jafar-Nejad,P. and Jafar-Nejad,H.
  TITLE     ASO silencing of a glycosyltransferase, Poglut1, improves the liver
            phenotypes in mouse models of Alagille syndrome
  JOURNAL   Hepatology 78 (5), 1337-1351 (2023)
   PUBMED   37021797
  REMARK    GeneRIF: ASO silencing of a glycosyltransferase, Poglut1 , improves
            the liver phenotypes in mouse models of Alagille syndrome.
REFERENCE   3  (bases 1 to 2835)
  AUTHORS   Thakurdas,S.M., Lopez,M.F., Kakuda,S., Fernandez-Valdivia,R.,
            Zarrin-Khameh,N., Haltiwanger,R.S. and Jafar-Nejad,H.
  TITLE     Jagged1 heterozygosity in mice results in a congenital
            cholangiopathy which is reversed by concomitant deletion of one
            copy of Poglut1 (Rumi)
  JOURNAL   Hepatology 63 (2), 550-565 (2016)
   PUBMED   26235536
  REMARK    GeneRIF: Removing one copy of Rumi suppresses the Jag1(+/-) bile
            duct phenotype, indicating that Rumi opposes JAG1 function in the
            liver
REFERENCE   4  (bases 1 to 2835)
  AUTHORS   Ramkumar,N., Harvey,B.M., Lee,J.D., Alcorn,H.L.,
            Silva-Gagliardi,N.F., McGlade,C.J., Bestor,T.H., Wijnholds,J.,
            Haltiwanger,R.S. and Anderson,K.V.
  TITLE     Protein O-Glucosyltransferase 1 (POGLUT1) Promotes Mouse
            Gastrulation through Modification of the Apical Polarity Protein
            CRUMBS2
  JOURNAL   PLoS Genet 11 (10), e1005551 (2015)
   PUBMED   26496195
  REMARK    GeneRIF: The data demonstrate that CRUMBS2 is the target of POGLUT1
            for the gastrulation epithelial-to-mesenchymal transitions (EMT)
            and that all activity of CRUMBS2 depends on modification by POGLUT1
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2835)
  AUTHORS   Takeuchi,H., Fernandez-Valdivia,R.C., Caswell,D.S., Nita-Lazar,A.,
            Rana,N.A., Garner,T.P., Weldeghiorghis,T.K., Macnaughtan,M.A.,
            Jafar-Nejad,H. and Haltiwanger,R.S.
  TITLE     Rumi functions as both a protein O-glucosyltransferase and a
            protein O-xylosyltransferase
  JOURNAL   Proc Natl Acad Sci U S A 108 (40), 16600-16605 (2011)
   PUBMED   21949356
REFERENCE   6  (bases 1 to 2835)
  AUTHORS   Fernandez-Valdivia,R., Takeuchi,H., Samarghandi,A., Lopez,M.,
            Leonardi,J., Haltiwanger,R.S. and Jafar-Nejad,H.
  TITLE     Regulation of mammalian Notch signaling and embryonic development
            by the protein O-glucosyltransferase Rumi
  JOURNAL   Development 138 (10), 1925-1934 (2011)
   PUBMED   21490058
  REMARK    GeneRIF: Rumi(-/-) embryos die before embryonic day 9.5 with
            posterior axis truncation and severe defects in neural tube
            development, somitogenesis, cardiogenesis and vascular remodeling.
REFERENCE   7  (bases 1 to 2835)
  AUTHORS   Garcia-Garcia,M.J., Eggenschwiler,J.T., Caspary,T., Alcorn,H.L.,
            Wyler,M.R., Huangfu,D., Rakeman,A.S., Lee,J.D., Feinberg,E.H.,
            Timmer,J.R. and Anderson,K.V.
  TITLE     Analysis of mouse embryonic patterning and morphogenesis by forward
            genetics
  JOURNAL   Proc Natl Acad Sci U S A 102 (17), 5913-5919 (2005)
   PUBMED   15755804
REFERENCE   8  (bases 1 to 2835)
  AUTHORS   Meunier,L., Usherwood,Y.K., Chung,K.T. and Hendershot,L.M.
  TITLE     A subset of chaperones and folding enzymes form multiprotein
            complexes in endoplasmic reticulum to bind nascent proteins
  JOURNAL   Mol Biol Cell 13 (12), 4456-4469 (2002)
   PUBMED   12475965
REFERENCE   9  (bases 1 to 2835)
  AUTHORS   Kasarskis,A., Manova,K. and Anderson,K.V.
  TITLE     A phenotype-based screen for embryonic lethal mutations in the
            mouse
  JOURNAL   Proc Natl Acad Sci U S A 95 (13), 7485-7490 (1998)
   PUBMED   9636176
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC209577.2, CB249259.1,
            AK036224.1 and AK079886.1.
            
            On Jul 15, 2014 this sequence version replaced NM_172380.3.
            
            Summary: This gene encodes a protein that can catalyze transfer of
            either UDP-glucose or UDP-xylose to epidermal growth factor (EGF)
            repeats, such as those found in Notch. Loss of this gene product
            results in embryonic lethality. Embryos have neural plate defects,
            heart defects, and truncations of their posterior axis. Alternate
            splicing results in multiple transcript variants. [provided by
            RefSeq, Jul 2014].
            
            Transcript Variant: This variant (1) represents the shorter
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC026809.1, AK036224.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164139
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 AC209577.2         5877-5878
            3-72                CB249259.1         4-73
            73-2438             AK036224.1         3-2368
            2439-2748           AK079886.1         2410-2719
            2749-2835           AC209577.2         30989-31075
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 26.86 cM"
     gene            1..2835
                     /gene="Poglut1"
                     /gene_synonym="9630046K23Rik; Clp46; Ktelc1; Rumi; wsnp"
                     /note="protein O-glucosyltransferase 1"
                     /db_xref="GeneID:224143"
                     /db_xref="MGI:MGI:2444232"
     exon            1..174
                     /gene="Poglut1"
                     /gene_synonym="9630046K23Rik; Clp46; Ktelc1; Rumi; wsnp"
                     /inference="alignment:Splign:2.1.0"
     CDS             90..1268
                     /gene="Poglut1"
                     /gene_synonym="9630046K23Rik; Clp46; Ktelc1; Rumi; wsnp"
                     /EC_number="2.4.2.63"
                     /EC_number="2.4.1.376"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; KTEL motif-containing protein 1; CAP10-like 46
                     kDa protein; KTEL (Lys-Tyr-Glu-Leu) containing 1;
                     O-glucosyltransferase Rumi homolog; wing-shaped neural
                     plate protein; protein O-xylosyltransferase POGLUT1"
                     /codon_start=1
                     /product="protein O-glucosyltransferase 1 isoform 1
                     precursor"
                     /protein_id="NP_759012.1"
                     /db_xref="CCDS:CCDS28170.1"
                     /db_xref="GeneID:224143"
                     /db_xref="MGI:MGI:2444232"
                     /translation="MERRAGSRLRAWMLLLLLCPVQGRQKDSGSKWKVFLDQINRALE
                     NYEPCSSQNCSCYHGVIEEDLTPFRGGISRKMMAEVVRRKLGTHYQIIKNRLFREDDC
                     MFPSRCSGVEHFILEVIHRLPDMEMVINVRDYPQVPKWMEPTIPVFSFSKTSEYHDIM
                     YPAWTFWEGGPAVWPLYPTGLGRWDLFREDLLRSAAQWPWEKKNSTAYFRGSRTSPER
                     DPLILLSRKNPKLVDAEYTKNQAWKSMKDTLGKPAAKDVHLIDHCKYRYLFNFRGVAA
                     SFRFKHLFLCGSLVFHVGDEWVEFFYPQLKPWVHYIPVKTDLSNVQELLQFVKANDDI
                     AQEIAKRGSQFIINHLQMDDITCYWENLLTDYSKFLSYNVTRRKDYYQIVPRRLKTEL
                     "
     exon            175..265
                     /gene="Poglut1"
                     /gene_synonym="9630046K23Rik; Clp46; Ktelc1; Rumi; wsnp"
                     /inference="alignment:Splign:2.1.0"
     exon            266..409
                     /gene="Poglut1"
                     /gene_synonym="9630046K23Rik; Clp46; Ktelc1; Rumi; wsnp"
                     /inference="alignment:Splign:2.1.0"
     exon            410..545
                     /gene="Poglut1"
                     /gene_synonym="9630046K23Rik; Clp46; Ktelc1; Rumi; wsnp"
                     /inference="alignment:Splign:2.1.0"
     exon            546..667
                     /gene="Poglut1"
                     /gene_synonym="9630046K23Rik; Clp46; Ktelc1; Rumi; wsnp"
                     /inference="alignment:Splign:2.1.0"
     exon            668..727
                     /gene="Poglut1"
                     /gene_synonym="9630046K23Rik; Clp46; Ktelc1; Rumi; wsnp"
                     /inference="alignment:Splign:2.1.0"
     exon            728..827
                     /gene="Poglut1"
                     /gene_synonym="9630046K23Rik; Clp46; Ktelc1; Rumi; wsnp"
                     /inference="alignment:Splign:2.1.0"
     exon            828..886
                     /gene="Poglut1"
                     /gene_synonym="9630046K23Rik; Clp46; Ktelc1; Rumi; wsnp"
                     /inference="alignment:Splign:2.1.0"
     exon            887..1054
                     /gene="Poglut1"
                     /gene_synonym="9630046K23Rik; Clp46; Ktelc1; Rumi; wsnp"
                     /inference="alignment:Splign:2.1.0"
     exon            1055..1111
                     /gene="Poglut1"
                     /gene_synonym="9630046K23Rik; Clp46; Ktelc1; Rumi; wsnp"
                     /inference="alignment:Splign:2.1.0"
     exon            1112..2835
                     /gene="Poglut1"
                     /gene_synonym="9630046K23Rik; Clp46; Ktelc1; Rumi; wsnp"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2756
                     /gene="Poglut1"
                     /gene_synonym="9630046K23Rik; Clp46; Ktelc1; Rumi; wsnp"
                     /note="major polyA site"
     regulatory      2805..2810
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Poglut1"
                     /gene_synonym="9630046K23Rik; Clp46; Ktelc1; Rumi; wsnp"
                     /note="hexamer: AATATA"
     regulatory      2815..2820
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Poglut1"
                     /gene_synonym="9630046K23Rik; Clp46; Ktelc1; Rumi; wsnp"
                     /note="hexamer: AATATA"
     polyA_site      2823
                     /gene="Poglut1"
                     /gene_synonym="9630046K23Rik; Clp46; Ktelc1; Rumi; wsnp"
     polyA_site      2833
                     /gene="Poglut1"
                     /gene_synonym="9630046K23Rik; Clp46; Ktelc1; Rumi; wsnp"
ORIGIN      
        1 tcccacgtgc cgtggcgtcc gccgccatcc ttctgctggg cggcgcttcc gccggcgtgc
       61 gggtgggaat ccgcagtggt ctgcgggtga tggagcgtcg ggcgggctct cggcttaggg
      121 catggatgct gttactgctc ctgtgcccgg tgcagggccg ccagaaggac tcaggctcaa
      181 aatggaaagt atttcttgac caaattaaca gggctttgga gaattatgaa ccatgttcaa
      241 gtcaaaactg cagctgctat cacggcgtca tagaagagga cctgactcct ttccgaggtg
      301 ggatctccag gaagatgatg gctgaggtgg tcaggcggaa gctaggaacc cactaccaga
      361 tcattaagaa ccggttattc agggaagatg actgcatgtt tccctccagg tgtagtggcg
      421 tggaacactt tattttggaa gtcatccatc gcctccctga catggaaatg gtaatcaatg
      481 tccgagatta tcctcaggtt cctaaatgga tggagcctac catccccgtc ttctccttca
      541 gtaagacatc ggagtaccat gatatcatgt atcctgcgtg gacattttgg gaagggggcc
      601 ctgctgtgtg gccactttat cctacaggtc ttggacggtg ggacctcttc agagaagacc
      661 tgttaaggtc agcagcgcaa tggccgtggg aaaagaaaaa ttctacagca tatttccgag
      721 gatcaaggac aagcccagaa agagatcctc tcattctcct atctcggaaa aatccaaagc
      781 tcgtcgatgc cgagtacacc aaaaaccagg cctggaagtc tatgaaagat actctgggaa
      841 agccagccgc taaggatgta cacctcatag atcactgcaa atacagatac ctatttaatt
      901 ttcgaggtgt agctgcaagc ttccggttca aacacctctt cttgtgcggt tcactggtct
      961 tccatgttgg tgatgagtgg gtggagttct tctacccaca actaaagcca tgggttcact
     1021 acatcccagt caagaccgac ctctccaatg tccaggagct gttgcagttt gtaaaagcga
     1081 atgatgatat cgctcaggaa attgctaaaa ggggaagcca gttcatcata aaccatttgc
     1141 agatggatga catcacctgt tactgggaga acctcttgac tgactattcc aaattcctgt
     1201 cctataatgt aacaagaaga aaagattatt atcagatcgt tcccagacgt ttgaaaactg
     1261 aactgtagta tccatcccag gaccagaatc ctgacagtgg atctgagatg ctctgtggtg
     1321 aaataccatg atcatgatgc ctgtaccttg aacacttttg gacaagccaa acatccagtt
     1381 ttccttatca tgttgaagcc agagggactc ttgagaaaga tctagtatgt gtgtaatgag
     1441 cagaaatgaa acagctcagc tctttggaat catgagataa ggctttggaa ctgcattcta
     1501 accatcgttt tcctatgatc agtcacagct tgtgcctcgg gtcatctacg tgtgttatca
     1561 ctgtgaaatg gactgtgtcc atctgaagat gctttgtcct gttatttgga gcagaccatc
     1621 catcgtttgg aactagtaca atacacaagc aactctgaag ttattttagg tttgattctg
     1681 ttgctttatt tttatgtaga aatcctttgg agtttgtgaa aaataccaca gcgattgctt
     1741 cctctggagt ctgaaggagg ccagtggctg tgctctgttt tgtaactgca taaattctct
     1801 ggcactcagg aggtttccat aatgcctcag tggcatgagt aacttctgca gttggatttc
     1861 agattcccat tttgtgcctt catgcccttt ttattctctc tgaaggagat taagagaaat
     1921 ttcccagtcc tttcaggaag cagtctcact aaagaactat gcctctgtaa tactagcact
     1981 tctgaagcga gaggcaggaa gatgaattcg tggccaccct cagctacaca ggagttcaag
     2041 gagagcccgg ctacatgagg tcctgtctca ccttttgcac atcttttatt ttgccatttt
     2101 gaatttgtag cagcaggatc agctcagttt gcagagactg cagtcttcat atgtgaccat
     2161 atcatgaagt ggcaatttat agagcactca gctttcgctt ttactccggt atgaggttat
     2221 ccttgggctc cagcatcacc tctgaacctc cttggcagtt ggcggctcac atctgtgttc
     2281 tgtgccaccg tgctgctttg tcgttacgcg cctctttccc ccacgctctg tacgcttact
     2341 gatggacgag aatgacgttt ctctgctctt gatgtctcca ttatcagcac agtgcctcac
     2401 gcagagcagt tactgaacag ctattggaat gagtgagtgg gaatggatgg atacactcgc
     2461 ctggcctaac taaagtctat tttttaaact gctcggttgc ttgctccagt ggggatgaga
     2521 tacactgtga gaaatgggtc agttcagatc cttacttgcc ctggagtagt aaggagtatc
     2581 tgtttttaaa aacaacaaaa gttactcggc tctgactctt gcctgtgtac ctcactgtag
     2641 atggtatgcg aatggtgggt aacagtatgt caagtgtgtg atttcgaaaa tggtttgtat
     2701 ggtcacactt ctgaagattt ctgttatcat taatactggt tcggtcctaa acgtaaccat
     2761 tggctcctca tttacttact atagaaaaga ctactattgt tttcaatata accgaatata
     2821 atatgttttg taaga
//
