LOCUS       NM_030262               2219 bp    mRNA    linear   ROD 01-OCT-2024
DEFINITION  Mus musculus protein O-fucosyltransferase 2 (Pofut2), mRNA.
ACCESSION   NM_030262
VERSION     NM_030262.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Berardinelli,S.J., Sillato,A.R., Grady,R.C., Neupane,S., Ito,A.,
            Haltiwanger,R.S. and Holdener,B.C.
  TITLE     O-fucosylation of thrombospondin type I repeats is dispensable for
            trafficking thrombospondin 1 to platelet secretory granules
  JOURNAL   Glycobiology 33 (4), 301-310 (2023)
   PUBMED   36721988
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Neupane,S., Berardinelli,S.J., Cameron,D.C., Grady,R.C.,
            Komatsu,D.E., Percival,C.J., Takeuchi,M., Ito,A., Liu,T.W.,
            Nairn,A.V., Moremen,K.W., Haltiwanger,R.S. and Holdener,B.C.
  TITLE     O-fucosylation of thrombospondin type 1 repeats is essential for
            ECM remodeling and signaling during bone development
  JOURNAL   Matrix Biol 107, 77-96 (2022)
   PUBMED   35167946
REFERENCE   3  (bases 1 to 2219)
  AUTHORS   Neupane,S., Goto,J., Berardinelli,S.J., Ito,A., Haltiwanger,R.S.
            and Holdener,B.C.
  TITLE     Hydrocephalus in mouse B3glct mutants is likely caused by defects
            in multiple B3GLCT substrates in ependymal cells and subcommissural
            organ
  JOURNAL   Glycobiology 31 (8), 988-1004 (2021)
   PUBMED   33909046
REFERENCE   4  (bases 1 to 2219)
  AUTHORS   Dubail,J., Vasudevan,D., Wang,L.W., Earp,S.E., Jenkins,M.W.,
            Haltiwanger,R.S. and Apte,S.S.
  TITLE     Impaired ADAMTS9 secretion: A potential mechanism for eye defects
            in Peters Plus Syndrome
  JOURNAL   Sci Rep 6, 33974 (2016)
   PUBMED   27687499
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2219)
  AUTHORS   Benz,B.A., Nandadasa,S., Takeuchi,M., Grady,R.C., Takeuchi,H.,
            LoPilato,R.K., Kakuda,S., Somerville,R.P.T., Apte,S.S.,
            Haltiwanger,R.S. and Holdener,B.C.
  TITLE     Genetic and biochemical evidence that gastrulation defects in
            Pofut2 mutants result from defects in ADAMTS9 secretion
  JOURNAL   Dev Biol 416 (1), 111-122 (2016)
   PUBMED   27297885
REFERENCE   6  (bases 1 to 2219)
  AUTHORS   Taniwaki,T., Haruna,K., Nakamura,H., Sekimoto,T., Oike,Y.,
            Imaizumi,T., Saito,F., Muta,M., Soejima,Y., Utoh,A., Nakagata,N.,
            Araki,M., Yamamura,K. and Araki,K.
  TITLE     Characterization of an exchangeable gene trap using pU-17 carrying
            a stop codon-beta geo cassette
  JOURNAL   Dev Growth Differ 47 (3), 163-172 (2005)
   PUBMED   15840001
REFERENCE   7  (bases 1 to 2219)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   8  (bases 1 to 2219)
  AUTHORS   Gitton,Y., Dahmane,N., Baik,S., Ruiz i Altaba,A., Neidhardt,L.,
            Scholze,M., Herrmann,B.G., Kahlem,P., Benkahla,A., Schrinner,S.,
            Yildirimman,R., Herwig,R., Lehrach,H. and Yaspo,M.L.
  CONSRTM   HSA21 expression map initiative
  TITLE     A gene expression map of human chromosome 21 orthologues in the
            mouse
  JOURNAL   Nature 420 (6915), 586-590 (2002)
   PUBMED   12466855
REFERENCE   9  (bases 1 to 2219)
  AUTHORS   Reymond,A., Marigo,V., Yaylaoglu,M.B., Leoni,A., Ucla,C.,
            Scamuffa,N., Caccioppoli,C., Dermitzakis,E.T., Lyle,R., Banfi,S.,
            Eichele,G., Antonarakis,S.E. and Ballabio,A.
  TITLE     Human chromosome 21 gene expression atlas in the mouse
  JOURNAL   Nature 420 (6915), 582-586 (2002)
   PUBMED   12466854
REFERENCE   10 (bases 1 to 2219)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CO814561.1, AF455270.1 and AK148244.1.
            
            On Feb 8, 2006 this sequence version replaced NM_030262.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK148244.1, AK132373.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-10                CO814561.1         1-10
            11-1332             AF455270.1         1-1322
            1333-2219           AK148244.1         1333-2219
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 39.72 cM"
     gene            1..2219
                     /gene="Pofut2"
                     /gene_synonym="2310011G23Rik; C21orf80; FUT13"
                     /note="protein O-fucosyltransferase 2"
                     /db_xref="GeneID:80294"
                     /db_xref="MGI:MGI:1916863"
     exon            1..154
                     /gene="Pofut2"
                     /gene_synonym="2310011G23Rik; C21orf80; FUT13"
                     /inference="alignment:Splign:2.1.0"
     CDS             24..1313
                     /gene="Pofut2"
                     /gene_synonym="2310011G23Rik; C21orf80; FUT13"
                     /EC_number="2.4.1.221"
                     /note="GDP-fucose protein O-fucosyltransferase 2;
                     O-FucT-2; peptide-O-fucosyltransferase 2"
                     /codon_start=1
                     /product="GDP-fucose protein O-fucosyltransferase 2
                     precursor"
                     /protein_id="NP_084538.2"
                     /db_xref="CCDS:CCDS23954.1"
                     /db_xref="GeneID:80294"
                     /db_xref="MGI:MGI:1916863"
                     /translation="MAALSVVCLLLAAASWRPVSASGEEFWPGQSAADILSGAASRRR
                     YLLYDVNPPEGFNLRRDVYIRVASLLKTLLKTEEWVLVLPPWGRLYHWQSPDIHQVRI
                     PWSEFFDLPSLNKNIPVIEYEQFIAESGGPFIDQVYVLQGYAEGWKEGTWEEKVDARP
                     CIDPLLYSQDKHEYYRGWFWGYEETRGLNVSCLSVQGSASIVAPVLLKNTSARSVMLD
                     RAENLLHDHYGGREYWDTRRSMVFAKHLRAVGDEFRSQHLNSTDAADKMAPEEDWTKM
                     KVKLGSALGGPYLGVHLRRKDFIWGHREDVPSLEGAVKKIRSLMKTHQLDKVFVATDA
                     IRKEQEELRKLLPEMVRFEPTWEELELYKDGGVAIIDQWICAHARFFIGTSVSTFSFR
                     IHEEREILGLDPKTTYNRFCGDQEKACEQPTHWKIAY"
     exon            155..405
                     /gene="Pofut2"
                     /gene_synonym="2310011G23Rik; C21orf80; FUT13"
                     /inference="alignment:Splign:2.1.0"
     exon            406..550
                     /gene="Pofut2"
                     /gene_synonym="2310011G23Rik; C21orf80; FUT13"
                     /inference="alignment:Splign:2.1.0"
     exon            551..661
                     /gene="Pofut2"
                     /gene_synonym="2310011G23Rik; C21orf80; FUT13"
                     /inference="alignment:Splign:2.1.0"
     exon            662..728
                     /gene="Pofut2"
                     /gene_synonym="2310011G23Rik; C21orf80; FUT13"
                     /inference="alignment:Splign:2.1.0"
     exon            729..854
                     /gene="Pofut2"
                     /gene_synonym="2310011G23Rik; C21orf80; FUT13"
                     /inference="alignment:Splign:2.1.0"
     exon            855..1035
                     /gene="Pofut2"
                     /gene_synonym="2310011G23Rik; C21orf80; FUT13"
                     /inference="alignment:Splign:2.1.0"
     exon            1036..1159
                     /gene="Pofut2"
                     /gene_synonym="2310011G23Rik; C21orf80; FUT13"
                     /inference="alignment:Splign:2.1.0"
     exon            1160..2219
                     /gene="Pofut2"
                     /gene_synonym="2310011G23Rik; C21orf80; FUT13"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2187..2192
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pofut2"
                     /gene_synonym="2310011G23Rik; C21orf80; FUT13"
                     /note="hexamer: AATAAA"
     polyA_site      2208
                     /gene="Pofut2"
                     /gene_synonym="2310011G23Rik; C21orf80; FUT13"
                     /note="major polyA site"
ORIGIN      
        1 ggaagtgcgt ggccgccggg gccatggcgg cgctcagcgt cgtctgcctg ctgctggcag
       61 cggcatcctg gcggccggtc tcggcttcag gagaagaatt ctggcctggc cagtcggcgg
      121 cggacatcct gtcgggggcg gcatcccgca gacggtacct cctgtatgat gtcaaccccc
      181 cagaaggctt caatctccgc agggatgtct atatccgggt ggcatccttg ctgaagacgc
      241 tgttgaagac tgaagagtgg gtgctagtcc tgcccccgtg gggccgcctc taccactggc
      301 aaagccctga catccaccag gtccggattc cctggtccga gttttttgac ctcccgagtc
      361 tcaataaaaa catccccgtt atagagtatg agcagttcat tgcagagtcc ggcgggccct
      421 ttatcgacca ggtgtacgtc ctacaaggtt acgccgaggg ctggaaggag ggcacctggg
      481 aggagaaggt ggacgcacgg ccctgtattg accctctgct gtactcgcag gacaagcatg
      541 agtattacag aggctggttt tggggctacg aagaaacacg gggtctgaat gtctcctgcc
      601 tgtccgtcca ggggtcggct tccatcgtcg cgcctgtcct cttaaaaaac acatctgctc
      661 ggtctgtgat gctggaccga gctgagaatc tgcttcatga ccactatgga ggccgggagt
      721 actgggatac ccggcgcagc atggtgtttg caaagcactt gcgggctgtg ggagacgagt
      781 ttaggagcca gcacctcaac tcgacggatg ccgctgataa gatggccccg gaggaagact
      841 ggacgaagat gaaggtcaaa ctgggctcag cactaggcgg cccctacctc ggagtccacc
      901 tgagaaggaa ggacttcatc tggggccaca gggaggatgt gcccagcctg gagggcgccg
      961 tgaagaagat ccgcagcctc atgaagactc atcagctgga caaggtgttc gtggccacag
     1021 acgccatcag gaaggagcag gaagaattga ggaagctgct gccggaaatg gtgaggtttg
     1081 agcccacgtg ggaggagctg gagctgtaca aggacggagg cgtcgccatc atcgatcagt
     1141 ggatctgcgc tcatgccagg ttttttattg gcacctctgt ttccacattt tcctttcgga
     1201 ttcatgaaga gagagagatc ctggggttgg accccaagac aacatacaac cggttttgtg
     1261 gagaccagga gaaagcatgt gagcagccca cacactggaa gattgcgtac tgagggccac
     1321 ctatccaccg cctgccaatc actcggtcac agggtcccag aggaggcccc cacatgggag
     1381 gcaacacatc tagagcccat gctcccacgt gtccgtgggc cgacatctac ctgtgtctag
     1441 gctctgccac cttcaggatt cacaactgtg tcttctctca ggacctgaga acccacgtcc
     1501 ccatcacaga tgtcacatgt gtgctagtcc agaaatagaa gaacatttgt cagctccgac
     1561 acaggcctct cagccacctt cctgaacaag ttgagcatgg ccagcctgtg gcaatgccct
     1621 caggtgtgga tgcgggctcc tgaggtgttg gggtgactga cgactgaggt cacttcacac
     1681 atggccagcg tgagccgaca ccaagccttg cctcagcctc cacctgtggg gctcccacta
     1741 atttcctcct ggatggaacg actcgggcac tgttgtcatc agagtgacag ccctggagcc
     1801 ttgccagtga cttcctcgcc tcacatggac ccccaggttc actgcacacg ctgagaaata
     1861 gaattgagaa gtaaaggaaa tggccttcaa aaggccacac ctgagacccg cccctgcttt
     1921 atgacaccct tccctgggcc tcatgctgta ccacaggagg cagagtcagc acagccgtgg
     1981 ccagggcacc cccaggtccc tgacctggta cttagcagtg cacccctccc cccagtgctg
     2041 tgcactgagc agcccccact cagctactgc caggtctccg tcccaagcac caaggtagac
     2101 tctgagcaca gacagggccc cgtggcccag tcagctgacc agctagaatc acatatttat
     2161 tttccatttg tacatccaag cgtattaata aaatactttt tcaagaaagc tgcagtcct
//
