LOCUS       NM_134021               2048 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus pyridoxine 5'-phosphate oxidase (Pnpo), mRNA.
ACCESSION   NM_134021
VERSION     NM_134021.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Chen,C.C., Li,B., Millman,S.E., Chen,C., Li,X., Morris,J.P. 4th,
            Mayle,A., Ho,Y.J., Loizou,E., Liu,H., Qin,W., Shah,H., Violante,S.,
            Cross,J.R., Lowe,S.W. and Zhang,L.
  TITLE     Vitamin B6 Addiction in Acute Myeloid Leukemia
  JOURNAL   Cancer Cell 37 (1), 71-84 (2020)
   PUBMED   31935373
REFERENCE   3  (bases 1 to 2048)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2048)
  AUTHORS   Kang,J.H., Hong,M.L., Kim,D.W., Park,J., Kang,T.C., Won,M.H.,
            Baek,N.I., Moon,B.J., Choi,S.Y. and Kwon,O.S.
  TITLE     Genomic organization, tissue distribution and deletion mutation of
            human pyridoxine 5'-phosphate oxidase
  JOURNAL   Eur J Biochem 271 (12), 2452-2461 (2004)
   PUBMED   15182361
REFERENCE   5  (bases 1 to 2048)
  AUTHORS   Ngo,E.O., LePage,G.R., Thanassi,J.W., Meisler,N. and Nutter,L.M.
  TITLE     Absence of pyridoxine-5'-phosphate oxidase (PNPO) activity in
            neoplastic cells: isolation, characterization, and expression of
            PNPO cDNA
  JOURNAL   Biochemistry 37 (21), 7741-7748 (1998)
   PUBMED   9601034
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK164667.1, CK625276.1, AL596384.17 and AI464320.1.
            
            On Feb 9, 2007 this sequence version replaced NM_134021.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC026564.1, AK154004.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849386, SAMN01164135
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-703               AK164667.1         2-704
            704-814             CK625276.1         513-623
            815-1851            AL596384.17        156758-157794       c
            1852-2048           AI464320.1         1-197               c
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 60.17 cM"
     gene            1..2048
                     /gene="Pnpo"
                     /note="pyridoxine 5'-phosphate oxidase"
                     /db_xref="GeneID:103711"
                     /db_xref="MGI:MGI:2144151"
     exon            1..289
                     /gene="Pnpo"
                     /inference="alignment:Splign:2.1.0"
     CDS             152..937
                     /gene="Pnpo"
                     /EC_number="1.4.3.5"
                     /note="pyridoxamine-phosphate oxidase"
                     /codon_start=1
                     /product="pyridoxine-5'-phosphate oxidase"
                     /protein_id="NP_598782.1"
                     /db_xref="CCDS:CCDS25308.1"
                     /db_xref="GeneID:103711"
                     /db_xref="MGI:MGI:2144151"
                     /translation="MTCGLLSVTVTFRRPAKWTGYLRHLCCRGAVMDLGPMRKSYRGD
                     REAFEETHLTSLDPMKQFASWFDEAVQCPDIGEANAMCVATCTRDGKPSARMLLLKGF
                     GKDGFRFFTNYESRKGKELDSNPFASLVFYWEPLNRQVRVEGPVKKLPEKEAENYFHS
                     RPKSSQIGAVVSRQSSVIPDREYLRKKNEELGQLYQDQEVPKPEYWGGYILYPQVMEF
                     WQGQTNRLHDRIVFRRGLATGDSPLGPMTHHGEEDWVYERLAP"
     exon            290..414
                     /gene="Pnpo"
                     /inference="alignment:Splign:2.1.0"
     exon            415..514
                     /gene="Pnpo"
                     /inference="alignment:Splign:2.1.0"
     exon            515..568
                     /gene="Pnpo"
                     /inference="alignment:Splign:2.1.0"
     exon            569..697
                     /gene="Pnpo"
                     /inference="alignment:Splign:2.1.0"
     exon            698..768
                     /gene="Pnpo"
                     /inference="alignment:Splign:2.1.0"
     exon            769..2036
                     /gene="Pnpo"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1998..2003
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pnpo"
                     /note="hexamer: AATAAA"
     polyA_site      2029
                     /gene="Pnpo"
                     /note="major polyA site"
ORIGIN      
        1 gatcctaggc accttggttc cgagaactta ggacttacta gcttgtctgg ggctctagta
       61 aattcttcct tcccaggcta gacacacagg ctgagcaatt ggttccgaac ccagggccag
      121 gcgacgctgg gtcacgtggc cgactgctta catgacgtgc gggctgctga gcgtcactgt
      181 gacgttcagg agacctgcga agtggacggg ctacctccgt cacctgtgct gtcggggtgc
      241 agtcatggac ctgggaccaa tgcgcaagag ttaccgcggg gatcgagagg cattcgagga
      301 gactcatctg acctctctgg atcccatgaa gcagtttgct tcctggtttg acgaggctgt
      361 tcagtgtccg gacataggag aagccaatgc tatgtgtgtg gctacctgca ccagagacgg
      421 aaaaccctct gcccggatgc tgctgctgaa gggctttggc aaagacggct tccgcttctt
      481 cactaactac gagagccgga agggaaaaga gctggattct aatccctttg cctctcttgt
      541 cttctactgg gagcccctga accgtcaggt gcgtgtggaa ggccctgtga agaaactccc
      601 agagaaggag gccgagaact acttccattc ccgccccaag agcagccaga ttggggctgt
      661 ggtcagtcgc cagagttctg tgatccctga ccgggagtac ctgagaaaga aaaatgagga
      721 actggggcag ctctaccagg accaagaggt acccaagcca gaatactggg gtggctacat
      781 cctgtacccc caagtgatgg agttttggca aggccaaacc aaccgcctcc atgaccgtat
      841 tgtcttccgc cgaggcctgg caacaggaga ctcccctttg ggacccatga cccaccacgg
      901 ggaggaagac tgggtgtatg agagacttgc accttaacac tggacccaag aatgttgggg
      961 aatcaccagg gggcagagag tggggaaaag cagttatggg aataagggaa tggaagcccg
     1021 gtctaccttc tctgtgtgtg gcgctgggtt taaaccttgc tcaggctcgg caactctgta
     1081 ccactgagtg agagtcccag catttctctc tctttacctt ttattttaag actaagttgc
     1141 tggcccctga gctgtttgga gaagtgcctg attggctcca ggagctgccg tggggggccc
     1201 tgtctctgct tcccctaggt tagccctgaa ctcactgtgt ttatatccca ggcaagcgtt
     1261 ggccttgagt tcctcttgcc tcagtccctg gagtagttga ttcttggctt gtgccaccag
     1321 gcccagctcc agttcttgtt tttaagctaa gccatttcct ttcttagccc ttctacctac
     1381 gtctctccgg gactcaccct ccaagttctc tgaactccga atcagttagt ggctggaaca
     1441 aagtgccgta gggttgtaga gaatcacaaa tagcaaagga tctcctaagt gttcccttga
     1501 ctttgctcct gggtttgggg gaagcctggc ttgggagctg tttccagtaa taaattaatc
     1561 aggctgcaga tagacaaaag gcttcccagc catggaaagg agccagccca ggccatgttt
     1621 gctgtgactc agacaaatca ttatatgttc tggttggcct tgaacttatc accctggaca
     1681 cacttgcctt gttttgactt tgtggctgtg tgcttcagcc tctgtcacta acaaaggctc
     1741 ctcccgaggc aaccttgtcc tgtgttgatc ccccacttca gcctgggtcc aggaaggaca
     1801 ttgaccctgt ggccatcggc caggtacctt tacctacctc accccttttg ctgatgtgcc
     1861 atggactgtg actttattag tataagccga tgagttgttg agaagtgtta taccccatgg
     1921 ctgttagggg gttgtgtgtg ctccctcccc cgggctttag tgtgatccca actgtcagtg
     1981 ccagtgcaca agggtgtaat aaaaacttgt cagccgtgtg tgatattaat gtttaaaaaa
     2041 aaaaaaaa
//
