LOCUS       NM_026164               4425 bp    mRNA    linear   ROD 12-NOV-2024
DEFINITION  Mus musculus patatin-like phospholipase domain containing 8
            (Pnpla8), mRNA.
ACCESSION   NM_026164
VERSION     NM_026164.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Cybulsky,A.V., Papillon,J., Guillemette,J.,
            Navarro-Betancourt,J.R., Elimam,H. and Fantus,I.G.
  TITLE     Genetic deletion of calcium-independent phospholipase A2gamma
            protects mice from diabetic nephropathy
  JOURNAL   PLoS One 19 (10), e0311404 (2024)
   PUBMED   39480824
  REMARK    GeneRIF: Genetic deletion of calcium-independent phospholipase
            A2gamma protects mice from diabetic nephropathy.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  AUTHORS   Moon,S.H., Dilthey,B.G., Guan,S., Sims,H.F., Pittman,S.K.,
            Keith,A.L., Jenkins,C.M., Weihl,C.C. and Gross,R.W.
  TITLE     Genetic deletion of skeletal muscle iPLA2gamma results in
            mitochondrial dysfunction, muscle atrophy and alterations in
            whole-body energy metabolism
  JOURNAL   iScience 26 (6), 106895 (2023)
   PUBMED   37275531
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4425)
  AUTHORS   Hirabayashi,T., Kawaguchi,M., Harada,S., Mouri,M., Takamiya,R.,
            Miki,Y., Sato,H., Taketomi,Y., Yokoyama,K., Kobayashi,T.,
            Tokuoka,S.M., Kita,Y., Yoda,E., Hara,S., Mikami,K., Nishito,Y.,
            Kikuchi,N., Nakata,R., Kaneko,M., Kiyonari,H., Kasahara,K.,
            Aiba,T., Ikeda,K., Soga,T., Kurano,M., Yatomi,Y. and Murakami,M.
  TITLE     Hepatic phosphatidylcholine catabolism driven by PNPLA7 and PNPLA8
            supplies endogenous choline to replenish the methionine cycle with
            methyl groups
  JOURNAL   Cell Rep 42 (2), 111940 (2023)
   PUBMED   36719796
  REMARK    GeneRIF: Hepatic phosphatidylcholine catabolism driven by PNPLA7
            and PNPLA8 supplies endogenous choline to replenish the methionine
            cycle with methyl groups.
REFERENCE   4  (bases 1 to 4425)
  AUTHORS   Liu,X., Sims,H.F., Jenkins,C.M., Guan,S., Dilthey,B.G. and
            Gross,R.W.
  TITLE     12-LOX catalyzes the oxidation of 2-arachidonoyl-lysolipids in
            platelets generating eicosanoid-lysolipids that are attenuated by
            iPLA2gamma knockout
  JOURNAL   J Biol Chem 295 (16), 5307-5320 (2020)
   PUBMED   32161117
REFERENCE   5  (bases 1 to 4425)
  AUTHORS   Yoda,E., Hachisu,K., Kuwata,H., Nakatani,Y. and Hara,S.
  TITLE     Gene Deletion of Calcium-Independent Phospholipase A2gamma
            (iPLA2gamma) Suppresses Adipogenic Differentiation of Mouse
            Embryonic Fibroblasts
  JOURNAL   Biol Pharm Bull 43 (9), 1375-1381 (2020)
   PUBMED   32879212
  REMARK    GeneRIF: Gene Deletion of Calcium-Independent Phospholipase A2gamma
            (iPLA2gamma) Suppresses Adipogenic Differentiation of Mouse
            Embryonic Fibroblasts.
REFERENCE   6  (bases 1 to 4425)
  AUTHORS   Mancuso,D.J., Kotzbauer,P., Wozniak,D.F., Sims,H.F., Jenkins,C.M.,
            Guan,S., Han,X., Yang,K., Sun,G., Malik,I., Conyers,S., Green,K.G.,
            Schmidt,R.E. and Gross,R.W.
  TITLE     Genetic ablation of calcium-independent phospholipase A2{gamma}
            leads to alterations in hippocampal cardiolipin content and
            molecular species distribution, mitochondrial degeneration,
            autophagy, and cognitive dysfunction
  JOURNAL   J Biol Chem 284 (51), 35632-35644 (2009)
   PUBMED   19840936
  REMARK    GeneRIF: the obligatory role of iPLA(2)gamma in neuronal
            mitochondrial lipid metabolism and membrane structure demonstrating
            that iPLA(2)gamma loss of function results in a mitochondrial
            neurodegenerative disorder
REFERENCE   7  (bases 1 to 4425)
  AUTHORS   Mancuso,D.J., Sims,H.F., Han,X., Jenkins,C.M., Guan,S.P., Yang,K.,
            Moon,S.H., Pietka,T., Abumrad,N.A., Schlesinger,P.H. and Gross,R.W.
  TITLE     Genetic ablation of calcium-independent phospholipase A2gamma leads
            to alterations in mitochondrial lipid metabolism and function
            resulting in a deficient mitochondrial bioenergetic phenotype
  JOURNAL   J Biol Chem 282 (48), 34611-34622 (2007)
   PUBMED   17923475
  REMARK    GeneRIF: iPLA(2)gamma is essential for maintaining efficient
            bioenergetic mitochondrial function through tailoring mitochondrial
            membrane lipid metabolism and composition
REFERENCE   8  (bases 1 to 4425)
  AUTHORS   Mancuso,D.J., Han,X., Jenkins,C.M., Lehman,J.J., Sambandam,N.,
            Sims,H.F., Yang,J., Yan,W., Yang,K., Green,K., Abendschein,D.R.,
            Saffitz,J.E. and Gross,R.W.
  TITLE     Dramatic accumulation of triglycerides and precipitation of cardiac
            hemodynamic dysfunction during brief caloric restriction in
            transgenic myocardium expressing human calcium-independent
            phospholipase A2gamma
  JOURNAL   J Biol Chem 282 (12), 9216-9227 (2007)
   PUBMED   17213206
REFERENCE   9  (bases 1 to 4425)
  AUTHORS   Song,K., Zhang,X., Zhao,C., Ang,N.T. and Ma,Z.A.
  TITLE     Inhibition of Ca2+-independent phospholipase A2 results in
            insufficient insulin secretion and impaired glucose tolerance
  JOURNAL   Mol Endocrinol 19 (2), 504-515 (2005)
   PUBMED   15471944
  REMARK    GeneRIF: iPLA2 signaling plays an important role in
            glucose-stimulated insulin secretion under physiological conditions
REFERENCE   10 (bases 1 to 4425)
  AUTHORS   Su,X., Mancuso,D.J., Bickel,P.E., Jenkins,C.M. and Gross,R.W.
  TITLE     Small interfering RNA knockdown of calcium-independent
            phospholipases A2 beta or gamma inhibits the hormone-induced
            differentiation of 3T3-L1 preadipocytes
  JOURNAL   J Biol Chem 279 (21), 21740-21748 (2004)
   PUBMED   15024020
  REMARK    GeneRIF: iPLA(2)beta and iPLA(2)gamma are required for the
            adipogenic program that drives resting 3T3-L1 cells into adipocytes
            after hormone stimulation
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ112890.1, AK145776.1, AK033938.1 and AB044139.1.
            
            On Nov 17, 2006 this sequence version replaced NM_026164.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB044139.1, SRR1660811.27848.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-91                CJ112890.1         3-93
            92-3464             AK145776.1         2-3374
            3465-4194           AK033938.1         541-1270
            4195-4425           AB044139.1         4070-4300
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 20.71 cM"
     gene            1..4425
                     /gene="Pnpla8"
                     /gene_synonym="1200006O19Rik; Ipla2(gamma)"
                     /note="patatin-like phospholipase domain containing 8"
                     /db_xref="GeneID:67452"
                     /db_xref="MGI:MGI:1914702"
     exon            1..148
                     /gene="Pnpla8"
                     /gene_synonym="1200006O19Rik; Ipla2(gamma)"
                     /inference="alignment:Splign:2.1.0"
     exon            149..1275
                     /gene="Pnpla8"
                     /gene_synonym="1200006O19Rik; Ipla2(gamma)"
                     /inference="alignment:Splign:2.1.0"
     CDS             238..2568
                     /gene="Pnpla8"
                     /gene_synonym="1200006O19Rik; Ipla2(gamma)"
                     /EC_number="3.1.1.5"
                     /note="intracellular membrane-associated
                     calcium-independent phospholipase A2 gamma; iPLA2-gamma;
                     patatin-like phospholipase domain-containing protein 8"
                     /codon_start=1
                     /product="calcium-independent phospholipase A2-gamma"
                     /protein_id="NP_080440.2"
                     /db_xref="CCDS:CCDS36436.1"
                     /db_xref="GeneID:67452"
                     /db_xref="MGI:MGI:1914702"
                     /translation="MSINLTLDIYIYFLNNARSLCGKQRSKQLHFVCSKQYWRMNHVN
                     VHREFHTSKKSCKWNRSEAHCSKHWHSPSNHGLHFGIVRLSTSAPKGLTKVSIHMSRI
                     KSTLNSVSKAIFGSQNEMVTRLAQFKPSSRILRKVSDKGWLKQKNVKQAVESLKNYSD
                     KSAGKNSLAEQKSYFADKEEDSGKHSLFHYTYGITTRFGESFSVLANHINSYFKSKGK
                     MSQTKEDKQLQDKPDLEERKSSSPGPDTVADRPDSESPLEVKDKLSSPTQMPEAHPVS
                     AKQSIANFLSRPTEGVQALVGGYIGGLVPKLKSDPKSPPEEQEVSAKTEQAVNKDKKA
                     EEKKRVLLQQEKIIARVSIDNRTRALVQALRRTADPKLCITRVEELTFHLLEFPEGKG
                     VAIKEKIIPYLLRLRQVKDETLQAAVREILALIGYVDPVKGRGIRILTIDGGGTRGVV
                     ALQTLRKLVELTQKPIHQLFDYICGVSTGAILAFMLGLFHMPLDECEELYRKLGSDVF
                     TQNVIVGTVKMSWSHAFYDSNTWEKILKDRIGSALMIETARNPACPKVAAISTIVNRG
                     QTPKAFVFRNYGHFPGTNSHYLGGCQYKMWQAIRASSAAPGYFAEYALGSDLHQDGGL
                     LLNNPSALALHECKCIWPDTPLECIVSLGTGRYESDVRNTSTYTSLKTKLSNVISSAT
                     DTEEVHIMLDGLLPSDTYFRFNPVICENIPLDESRDEKLDQLQLEGMKYIERNDQKMK
                     KVAKILSQEKTTLQKINDWIKLKSDMYEGLPFFSKL"
     exon            1276..1425
                     /gene="Pnpla8"
                     /gene_synonym="1200006O19Rik; Ipla2(gamma)"
                     /inference="alignment:Splign:2.1.0"
     exon            1426..1577
                     /gene="Pnpla8"
                     /gene_synonym="1200006O19Rik; Ipla2(gamma)"
                     /inference="alignment:Splign:2.1.0"
     exon            1578..1672
                     /gene="Pnpla8"
                     /gene_synonym="1200006O19Rik; Ipla2(gamma)"
                     /inference="alignment:Splign:2.1.0"
     exon            1673..1844
                     /gene="Pnpla8"
                     /gene_synonym="1200006O19Rik; Ipla2(gamma)"
                     /inference="alignment:Splign:2.1.0"
     exon            1845..1902
                     /gene="Pnpla8"
                     /gene_synonym="1200006O19Rik; Ipla2(gamma)"
                     /inference="alignment:Splign:2.1.0"
     exon            1903..2097
                     /gene="Pnpla8"
                     /gene_synonym="1200006O19Rik; Ipla2(gamma)"
                     /inference="alignment:Splign:2.1.0"
     exon            2098..2293
                     /gene="Pnpla8"
                     /gene_synonym="1200006O19Rik; Ipla2(gamma)"
                     /inference="alignment:Splign:2.1.0"
     exon            2294..4406
                     /gene="Pnpla8"
                     /gene_synonym="1200006O19Rik; Ipla2(gamma)"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3042..3047
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pnpla8"
                     /gene_synonym="1200006O19Rik; Ipla2(gamma)"
                     /note="hexamer: ATTAAA"
     polyA_site      3075
                     /gene="Pnpla8"
                     /gene_synonym="1200006O19Rik; Ipla2(gamma)"
                     /note="major polyA site"
     regulatory      3240..3245
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pnpla8"
                     /gene_synonym="1200006O19Rik; Ipla2(gamma)"
                     /note="hexamer: AATAAA"
     polyA_site      3275
                     /gene="Pnpla8"
                     /gene_synonym="1200006O19Rik; Ipla2(gamma)"
     polyA_site      3466
                     /gene="Pnpla8"
                     /gene_synonym="1200006O19Rik; Ipla2(gamma)"
                     /note="major polyA site"
     regulatory      3638..3643
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pnpla8"
                     /gene_synonym="1200006O19Rik; Ipla2(gamma)"
                     /note="hexamer: CATAAA"
     polyA_site      3656
                     /gene="Pnpla8"
                     /gene_synonym="1200006O19Rik; Ipla2(gamma)"
     regulatory      4272..4277
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pnpla8"
                     /gene_synonym="1200006O19Rik; Ipla2(gamma)"
                     /note="hexamer: AGTAAA"
     polyA_site      4298
                     /gene="Pnpla8"
                     /gene_synonym="1200006O19Rik; Ipla2(gamma)"
     regulatory      4369..4374
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pnpla8"
                     /gene_synonym="1200006O19Rik; Ipla2(gamma)"
                     /note="hexamer: AATAAA"
     polyA_site      4406
                     /gene="Pnpla8"
                     /gene_synonym="1200006O19Rik; Ipla2(gamma)"
ORIGIN      
        1 ggggcgtagc ggtgggtggt gctggtcacg ccagtgtttg gggtccgggc gccgcagctg
       61 cgagcaggcc gggcgggccg atgcggacct cagagctggc gggaggcgag tggcggccgc
      121 ggcgcccgtg actactgcct gcgtcccggt aagaccatga attatggaat ttcctaaatg
      181 aagcattcag gaatggaatg agagaatgtc acatagaaaa taaatgattt ttaagttatg
      241 tctattaatt tgactctaga tatatatatt tacttcctta ataatgcaag aagtctttgt
      301 gggaaacaga gaagcaaaca attgcatttt gtgtgttcta aacagtattg gcgcatgaac
      361 catgtcaatg tgcacagaga gtttcacaca agtaagaaaa gctgtaaatg gaatagaagt
      421 gaagcacatt gcagcaaaca ctggcactct ccaagcaacc atggcttaca ctttggaatt
      481 gtgagactta gcacttctgc tcccaaggga cttacaaaag tgagcattca tatgtcccgt
      541 attaaaagta ctttgaattc agtttcaaag gctatttttg gcagtcaaaa tgaaatggtt
      601 acacggttag ctcaatttaa gccaagttct cgaatattaa gaaaagtatc agataaggga
      661 tggttaaaac aaaaaaatgt taagcaagcc gtcgagtctc tgaaaaacta tagtgataaa
      721 tcagcaggaa agaattcttt agcagaacag aaaagttact ttgcagataa agaagaagat
      781 tcaggtaaac acagcctttt ccattacaca tacggtataa ccaccagatt tggagagtca
      841 ttctccgttt tagcaaatca tattaattcc tatttcaaaa gcaagggaaa aatgtctcaa
      901 acaaaggaag ataaacagct tcaggacaag ccagaccttg aagaaaggaa atccagttct
      961 ccaggccctg acaccgtggc agacaggcca gactcggaat ctcccctaga agttaaagac
     1021 aaactttcca gcccgaccca gatgcctgag gctcacccag tgtctgcaaa acaaagtatt
     1081 gctaacttcc tttctcgtcc cactgaaggc gtacaagcct tagtcggtgg ttacattggt
     1141 ggacttgtac ccaaattaaa gtctgatccc aagagtccgc ccgaagaaca ggaagtgtct
     1201 gctaaaacag agcaggctgt taacaaagac aagaaggcag aggagaaaaa gcgtgtgcta
     1261 cttcagcaag aaaagattat tgcaagagtg agtattgata acagaactcg agccttagtt
     1321 caggccttaa gaagaacagc cgacccaaag ctctgtatta ctagggttga agaactgact
     1381 tttcatcttc tagaatttcc tgaaggaaaa ggagtggcta tcaaggaaaa aattattcca
     1441 tatttattac ggctgagaca agttaaggat gaaactcttc aggctgcagt tagagaaatt
     1501 ttggctttaa ttggttatgt agatccagtg aaaggcagag gaatccgaat cctcaccatt
     1561 gatggtggag gaacaagagg tgtggttgct ctccagactc tgaggaagtt ggttgaactt
     1621 actcagaagc cgattcatca gctttttgat tacatctgtg gagtaagcac aggggccata
     1681 ttagcattta tgctgggatt gtttcacatg ccgctggatg aatgtgagga actctatcga
     1741 aagttgggct cagatgtgtt cacacagaat gtcattgtcg ggaccgtcaa aatgagttgg
     1801 agccatgcgt tttatgacag taacacgtgg gaaaagatcc tcaaggatag aattggatct
     1861 gcactaatga ttgaaacagc gagaaacccc gcatgtccta aggtagctgc tataagtacc
     1921 atagtaaaca gaggacaaac accaaaagct tttgtgttca gaaactatgg tcattttcct
     1981 ggcaccaact ctcactattt aggaggctgt cagtataaaa tgtggcaggc cattagagcc
     2041 tcatcagctg ctccaggcta ctttgcagag tacgcgctgg ggagtgatct tcatcaagat
     2101 ggaggtttgc ttctgaataa cccttcggcc ttggcccttc acgaatgtaa atgcatctgg
     2161 ccagatacgc cactggagtg tatagtatcc ttgggcacag gacggtatga gagtgatgtg
     2221 aggaatacct caacatacac gagcctgaag accaagctct ctaatgttat cagcagtgct
     2281 acagatacag aggaagttca tatcatgctt gatggcctgt tgccttctga cacgtacttt
     2341 agatttaatc ctgtgatatg tgaaaacata cccctagatg aaagccggga tgaaaaactg
     2401 gaccagcttc agctggaagg gatgaagtat atagaaagaa atgatcaaaa gatgaaaaaa
     2461 gttgcaaaaa tattaagtca agaaaaaaca actcttcaga agatcaatga ttggataaaa
     2521 ttaaaaagtg acatgtatga aggccttcca tttttttcaa aattgtgagt agggtacaca
     2581 tgtgttctca taaatgaagg ccatcaaaag atccaccagt ttagagagga acggtgtgat
     2641 tcaacatgac acttagtgag ttatcctgaa tcagataatg ctttgaacag ctccatcctc
     2701 atggaagata tgattggtaa cacaagttat gtgggaatta ggtattaaga tgttcataac
     2761 ctttaggcat tttattactg tgctagtggt tttagtatct attgatctta agttgtttac
     2821 tgtttgaatg ttttaatata tgtgccaaac aagaaatggg ggatgttaca cttcagcctt
     2881 tgcttttact cgtttcaatc atgtggaatt tgtgtgactt gattttcttt catgtctttc
     2941 catttacact acttagcatt tcactaggtg cgcccacaat ccatgtgtta cttttcactg
     3001 taataatcac tgaaagagat ataggaaatg ggattttctt tattaaagta catttatgtt
     3061 tgaaatatga aaaaactgga cagttttcta ttcaaaaaga ttgaagaatt tccagtgaac
     3121 taactttttc ttgaaatttc aagataatgt tcacttttgt agatcaaatg ctcttatcat
     3181 ttcctgttac agaattgatt attcaatttt actgagaaaa tattcttatc attaacaaaa
     3241 ataaattatt gaagtgacca attgttcaag gctaatgcca ttttaacgtt tgtattttaa
     3301 ttgctcataa tggttgcctg gtttcttgta gcatttatga agaaatatta gtctagttag
     3361 atttacaaaa gttgaacatg gtgccataag tattcactgt attgttcact gtattatttc
     3421 tcttgtaaat ctttatattg aattgaaaat ttttatgaaa agttaatcaa ggtgtatatc
     3481 ctttgcttac tacacataac taacagtgct gcttccgttg tcattgtgct tgtgagagct
     3541 gggagtggtt gagaatgtgg gaggtagtcg gctgaatagt cagcccctgt gcatgacctt
     3601 gaagacagtg gacagcttgt aagattcagc tctgattcat aaaaggagga aagctagttg
     3661 tcaaatttca tttcttctcc tttatttttt aagaactaga tcaataagct cccagttatt
     3721 tcagaagata atgtaaatac ttccttttag cttttaacta caaacaaact tttatgtagc
     3781 cagactgact tctgaaaaat agaagctaat ggtaatctgc acagcccggg attctctgtg
     3841 agcgcagatt tcaagcacaa gctacttact gtgtggcccg tgtaagtgtg ctcttgtcct
     3901 cacctggaat atatagttca tgctttgcca cctaagtcca ctcttcctgg tttgtgcttt
     3961 gctaggcatt gctaagggta ctcaggtcag gccacagtgt ttttgttcag ccacatattg
     4021 ggtgccacgt atgaactttc cttctaacta agtatggcca tttgtgtgtt agacaggttt
     4081 ttgtttattc ttttctccat tggtatctga tactggttat ctaaagagct gagtaagtgt
     4141 ctgatacaac ctttatttta taaagaagga agtgctcttt gcccaaaaat cattctaaaa
     4201 gcagcctgta aaagagagac atagaaatga gtttttagtc ttatgcagat taaagtcttt
     4261 ctcagagaag cagtaaaatc tgttttatga aatacaaact catcagttgt actaaagtat
     4321 tattttgatc tcagaccttg ctgactgttt aagaaatcaa tttttaaaaa taaataacag
     4381 atagtattct ttattaagca tcaaaaaaaa aaaaaaaaaa aaaaa
//
