LOCUS       NM_146251               4592 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus patatin-like phospholipase domain containing 7
            (Pnpla7), mRNA.
ACCESSION   NM_146251
VERSION     NM_146251.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Harada,S., Taketomi,Y., Aiba,T., Kawaguchi,M., Hirabayashi,T.,
            Uranbileg,B., Kurano,M., Yatomi,Y. and Murakami,M.
  TITLE     The Lysophospholipase PNPLA7 Controls Hepatic Choline and
            Methionine Metabolism
  JOURNAL   Biomolecules 13 (3), 471 (2023)
   PUBMED   36979406
  REMARK    GeneRIF: The Lysophospholipase PNPLA7 Controls Hepatic Choline and
            Methionine Metabolism.
            Review article
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  AUTHORS   Hirabayashi,T., Kawaguchi,M., Harada,S., Mouri,M., Takamiya,R.,
            Miki,Y., Sato,H., Taketomi,Y., Yokoyama,K., Kobayashi,T.,
            Tokuoka,S.M., Kita,Y., Yoda,E., Hara,S., Mikami,K., Nishito,Y.,
            Kikuchi,N., Nakata,R., Kaneko,M., Kiyonari,H., Kasahara,K.,
            Aiba,T., Ikeda,K., Soga,T., Kurano,M., Yatomi,Y. and Murakami,M.
  TITLE     Hepatic phosphatidylcholine catabolism driven by PNPLA7 and PNPLA8
            supplies endogenous choline to replenish the methionine cycle with
            methyl groups
  JOURNAL   Cell Rep 42 (2), 111940 (2023)
   PUBMED   36719796
  REMARK    GeneRIF: Hepatic phosphatidylcholine catabolism driven by PNPLA7
            and PNPLA8 supplies endogenous choline to replenish the methionine
            cycle with methyl groups.
REFERENCE   3  (bases 1 to 4592)
  AUTHORS   Wang,X., Guo,M., Wang,Q., Wang,Q., Zuo,S., Zhang,X., Tong,H.,
            Chen,J., Wang,H., Chen,X., Guo,J., Su,X., Liang,H., Zhou,H. and
            Li,J.Z.
  TITLE     The Patatin-Like Phospholipase Domain Containing Protein 7
            Facilitates VLDL Secretion by Modulating ApoE Stability
  JOURNAL   Hepatology 72 (5), 1569-1585 (2020)
   PUBMED   32103509
  REMARK    GeneRIF: The Patatin-Like Phospholipase Domain Containing Protein 7
            Facilitates VLDL Secretion by Modulating ApoE Stability.
REFERENCE   4  (bases 1 to 4592)
  AUTHORS   Heier,C., Kien,B., Huang,F., Eichmann,T.O., Xie,H., Zechner,R. and
            Chang,P.A.
  TITLE     The phospholipase PNPLA7 functions as a lysophosphatidylcholine
            hydrolase and interacts with lipid droplets through its catalytic
            domain
  JOURNAL   J Biol Chem 292 (46), 19087-19098 (2017)
   PUBMED   28887301
REFERENCE   5  (bases 1 to 4592)
  AUTHORS   Chang,P.A., Wang,Z.X., Long,D.X., Qin,W.Z., Wei,C.Y. and Wu,Y.J.
  TITLE     Identification of two novel splicing variants of murine NTE-related
            esterase
  JOURNAL   Gene 497 (2), 164-171 (2012)
   PUBMED   22326266
  REMARK    GeneRIF: There was a nutritional regulation of murine NTE-related
            esterase expression at the mRNA levels via alternative splicing.
REFERENCE   6  (bases 1 to 4592)
  AUTHORS   Kienesberger,P.C., Lass,A., Preiss-Landl,K., Wolinski,H.,
            Kohlwein,S.D., Zimmermann,R. and Zechner,R.
  TITLE     Identification of an insulin-regulated lysophospholipase with
            homology to neuropathy target esterase
  JOURNAL   J Biol Chem 283 (9), 5908-5917 (2008)
   PUBMED   18086666
REFERENCE   7  (bases 1 to 4592)
  AUTHORS   Chang,P.A., Long,D.X. and Wu,Y.J.
  TITLE     Molecular cloning and expression of the C-terminal domain of mouse
            NTE-related esterase
  JOURNAL   Mol Cell Biochem 306 (1-2), 25-32 (2007)
   PUBMED   17673953
REFERENCE   8  (bases 1 to 4592)
  AUTHORS   Blackshaw,S., Harpavat,S., Trimarchi,J., Cai,L., Huang,H.,
            Kuo,W.P., Weber,G., Lee,K., Fraioli,R.E., Cho,S.H., Yung,R.,
            Asch,E., Ohno-Machado,L., Wong,W.H. and Cepko,C.L.
  TITLE     Genomic analysis of mouse retinal development
  JOURNAL   PLoS Biol 2 (9), E247 (2004)
   PUBMED   15226823
REFERENCE   9  (bases 1 to 4592)
  AUTHORS   Winrow,C.J., Hemming,M.L., Allen,D.M., Quistad,G.B., Casida,J.E.
            and Barlow,C.
  TITLE     Loss of neuropathy target esterase in mice links organophosphate
            exposure to hyperactivity
  JOURNAL   Nat Genet 33 (4), 477-485 (2003)
   PUBMED   12640454
REFERENCE   10 (bases 1 to 4592)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB852360.1, BB618610.1, BC141366.1, BE623320.1 and AI505242.1.
            
            On Mar 17, 2009 this sequence version replaced NM_146251.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK148380.1, AK088362.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-178               BB852360.1         2-179
            179-216             BB618610.1         153-190
            217-4543            BC141366.1         1-4327
            4544-4574           BE623320.1         4-34                c
            4575-4592           AI505242.1         1-18                c
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 16.88 cM"
     gene            1..4592
                     /gene="Pnpla7"
                     /gene_synonym="E430013P11Rik; Nre"
                     /note="patatin-like phospholipase domain containing 7"
                     /db_xref="GeneID:241274"
                     /db_xref="MGI:MGI:2385325"
     exon            1..281
                     /gene="Pnpla7"
                     /gene_synonym="E430013P11Rik; Nre"
                     /inference="alignment:Splign:2.1.0"
     CDS             252..4310
                     /gene="Pnpla7"
                     /gene_synonym="E430013P11Rik; Nre"
                     /EC_number="3.1.1.5"
                     /note="NTE-related esterase; neuropathy target
                     esterase-related esterase"
                     /codon_start=1
                     /product="patatin-like phospholipase domain-containing
                     protein 7"
                     /protein_id="NP_666363.3"
                     /db_xref="CCDS:CCDS50522.1"
                     /db_xref="GeneID:241274"
                     /db_xref="MGI:MGI:2385325"
                     /translation="MQNEEDACLEAGYCLGTTLSSWRLHFMEEQSQSTMLMGIGIGAL
                     LTLAFVGITFFFVYRRVRRLRRAEPTPQYRFRKRDKVMFYGRKIMRKVTTLPHTLVGN
                     TSAPRQRVRKRTKVLSLAKRILRFKKEYPTLQPKEPPPSLLEADLTEFDVKNSHLPSE
                     VLYMLKNVRVLGHFEKPLFLELCKHMVFVQLQEGEHVFQPGEPDISIYVVQDGRLEVC
                     IQDADGTEVVVKEVLPGDSVHSLLSILDVITGHTAPYKTVSARAAVSSTVLWLPAAAF
                     QGVFEKYPETLVRVVQIIMVRLQRVTFLALHNYLGLTTELFNPESQAIPLLSVASVAG
                     RAKRQMSYGPEEQLERSLRPSEFSSSDHGSSCVTVSGPLLKRSCSVPLPSNHGEVDEL
                     RQSQGSGSNTSAFQESHEGATSDLGMAYNRARILPHSDEQLGNSLASKSKKSVVAETP
                     SAIFHYSENFRDETGACGKTDAIFRAATKDLLTLMKLDDPSLLDGRVAFLHVPAGTLV
                     SKQGDQDVNILFVVSGMLHVYQQKIDSLEDTCLFLTHPGEMVGQLAVLTGEPLMFTIR
                     ANRDCSFLSISKAHFYEIMRKRPDVVLGVAHTVVKRMSSFVRQIDFALDWMEVEAGRA
                     IYRQGDKSDCTYIVLSGRLRSVIRKDDGKKRLAGEYGRGDLVGVVETLTHQARATTVH
                     AVRDSELAKLPAGALTSIKRRYPQVVTRLIHLLGEKILGSLQQGSATGHQLGFNTASS
                     KWDLGNPPGNLSTVAALPASEDVPLTAFALELQHALSAIGPVLLLTSDNIKQRLGSAA
                     LDSIHEYRLSSWLGQQEDIHRIVLYQADGTLTPWTQRCIRQADCILIVGLGEQEPAVG
                     ELEQMLESTAVRAQKQLILLHKEDGPVPSRTVEWLNMRSWCSGHLHLCCPRRVFSKRS
                     LPKLVEMYTRVFQRPPDRHSDFSRLARMLTGNAIALVLGGGGARGCAQVGILRALAEC
                     GVPVDIIGGTSIGAFMGALFAEERSYSQTRIRAKQWAEGMTSMMKTILDLTYPITSMF
                     SGTGFNSSISNIFKDRQIEDLWLPYFAITTDITASAMRVHTDGSLWRYVRASMSLSGY
                     MPPLCDPKDGHLLMDGGYINNLPADVARSMGAKVVIAIDVGSRDETDLTNYGDALSGW
                     WLLWKRWNPLATKVKVLNMAEIQTRLAYVCCVRQLEMVKNSDYCEYLRPPIDSYRTLD
                     FGKFDEICEVGYQHGRTVFDIWVRSGVLEKMLQDQQGTSKRKDCGVFTCPNSSFTDLA
                     EIVSRIEPAKVAAVDDESDYQTEYEEELPAIPKETYADFQSTGIELDSDSEYEPSMLQ
                     GPPSLTSPEQSQDSFPWLPNQDDQGPRLEHPS"
     exon            282..356
                     /gene="Pnpla7"
                     /gene_synonym="E430013P11Rik; Nre"
                     /inference="alignment:Splign:2.1.0"
     exon            357..444
                     /gene="Pnpla7"
                     /gene_synonym="E430013P11Rik; Nre"
                     /inference="alignment:Splign:2.1.0"
     exon            445..524
                     /gene="Pnpla7"
                     /gene_synonym="E430013P11Rik; Nre"
                     /inference="alignment:Splign:2.1.0"
     exon            525..616
                     /gene="Pnpla7"
                     /gene_synonym="E430013P11Rik; Nre"
                     /inference="alignment:Splign:2.1.0"
     exon            617..757
                     /gene="Pnpla7"
                     /gene_synonym="E430013P11Rik; Nre"
                     /inference="alignment:Splign:2.1.0"
     exon            758..917
                     /gene="Pnpla7"
                     /gene_synonym="E430013P11Rik; Nre"
                     /inference="alignment:Splign:2.1.0"
     exon            918..998
                     /gene="Pnpla7"
                     /gene_synonym="E430013P11Rik; Nre"
                     /inference="alignment:Splign:2.1.0"
     exon            999..1127
                     /gene="Pnpla7"
                     /gene_synonym="E430013P11Rik; Nre"
                     /inference="alignment:Splign:2.1.0"
     exon            1128..1208
                     /gene="Pnpla7"
                     /gene_synonym="E430013P11Rik; Nre"
                     /inference="alignment:Splign:2.1.0"
     exon            1209..1332
                     /gene="Pnpla7"
                     /gene_synonym="E430013P11Rik; Nre"
                     /inference="alignment:Splign:2.1.0"
     exon            1333..1470
                     /gene="Pnpla7"
                     /gene_synonym="E430013P11Rik; Nre"
                     /inference="alignment:Splign:2.1.0"
     exon            1471..1571
                     /gene="Pnpla7"
                     /gene_synonym="E430013P11Rik; Nre"
                     /inference="alignment:Splign:2.1.0"
     exon            1572..1715
                     /gene="Pnpla7"
                     /gene_synonym="E430013P11Rik; Nre"
                     /inference="alignment:Splign:2.1.0"
     exon            1716..1793
                     /gene="Pnpla7"
                     /gene_synonym="E430013P11Rik; Nre"
                     /inference="alignment:Splign:2.1.0"
     exon            1794..1999
                     /gene="Pnpla7"
                     /gene_synonym="E430013P11Rik; Nre"
                     /inference="alignment:Splign:2.1.0"
     exon            2000..2131
                     /gene="Pnpla7"
                     /gene_synonym="E430013P11Rik; Nre"
                     /inference="alignment:Splign:2.1.0"
     exon            2132..2255
                     /gene="Pnpla7"
                     /gene_synonym="E430013P11Rik; Nre"
                     /inference="alignment:Splign:2.1.0"
     exon            2256..2369
                     /gene="Pnpla7"
                     /gene_synonym="E430013P11Rik; Nre"
                     /inference="alignment:Splign:2.1.0"
     exon            2370..2439
                     /gene="Pnpla7"
                     /gene_synonym="E430013P11Rik; Nre"
                     /inference="alignment:Splign:2.1.0"
     exon            2440..2589
                     /gene="Pnpla7"
                     /gene_synonym="E430013P11Rik; Nre"
                     /inference="alignment:Splign:2.1.0"
     exon            2590..2653
                     /gene="Pnpla7"
                     /gene_synonym="E430013P11Rik; Nre"
                     /inference="alignment:Splign:2.1.0"
     exon            2654..2822
                     /gene="Pnpla7"
                     /gene_synonym="E430013P11Rik; Nre"
                     /inference="alignment:Splign:2.1.0"
     exon            2823..3005
                     /gene="Pnpla7"
                     /gene_synonym="E430013P11Rik; Nre"
                     /inference="alignment:Splign:2.1.0"
     exon            3006..3124
                     /gene="Pnpla7"
                     /gene_synonym="E430013P11Rik; Nre"
                     /inference="alignment:Splign:2.1.0"
     exon            3125..3281
                     /gene="Pnpla7"
                     /gene_synonym="E430013P11Rik; Nre"
                     /inference="alignment:Splign:2.1.0"
     exon            3282..3398
                     /gene="Pnpla7"
                     /gene_synonym="E430013P11Rik; Nre"
                     /inference="alignment:Splign:2.1.0"
     exon            3399..3468
                     /gene="Pnpla7"
                     /gene_synonym="E430013P11Rik; Nre"
                     /inference="alignment:Splign:2.1.0"
     exon            3469..3585
                     /gene="Pnpla7"
                     /gene_synonym="E430013P11Rik; Nre"
                     /inference="alignment:Splign:2.1.0"
     exon            3586..3734
                     /gene="Pnpla7"
                     /gene_synonym="E430013P11Rik; Nre"
                     /inference="alignment:Splign:2.1.0"
     exon            3735..3887
                     /gene="Pnpla7"
                     /gene_synonym="E430013P11Rik; Nre"
                     /inference="alignment:Splign:2.1.0"
     exon            3888..3998
                     /gene="Pnpla7"
                     /gene_synonym="E430013P11Rik; Nre"
                     /inference="alignment:Splign:2.1.0"
     exon            3999..4083
                     /gene="Pnpla7"
                     /gene_synonym="E430013P11Rik; Nre"
                     /inference="alignment:Splign:2.1.0"
     exon            4084..4187
                     /gene="Pnpla7"
                     /gene_synonym="E430013P11Rik; Nre"
                     /inference="alignment:Splign:2.1.0"
     exon            4188..4589
                     /gene="Pnpla7"
                     /gene_synonym="E430013P11Rik; Nre"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4557..4562
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pnpla7"
                     /gene_synonym="E430013P11Rik; Nre"
                     /note="hexamer: AATAAA"
     polyA_site      4574
                     /gene="Pnpla7"
                     /gene_synonym="E430013P11Rik; Nre"
                     /note="major polyA site"
ORIGIN      
        1 agcagttgtg ggttcccctt agaccacgtg agggctgttt acaggagacg gaagcacaca
       61 gagaaagcag acgcttctgg aaaacaactt gttttggagc acagagttct gaagtctctt
      121 ccaataagag cgtttgtgtg gaaaggagct ggcaagactg gaactacagc tgtcctgctg
      181 taaaggttcg ggtacagcag tttgtctcag cctgtctcct tttcgccctc cacattgctt
      241 tgctttcagt catgcagaat gaggaagatg cctgccttga ggctggctac tgtttgggta
      301 ccaccctgag ctcctggagg ctgcatttca tggaggagca gtcccagtcc accatgctga
      361 tggggattgg aattggcgcc ctccttaccc tagccttcgt tggtattacc ttcttttttg
      421 tatacagaag agtgagaaga ttacgacggg cagaacccac tcctcagtac aggttccgga
      481 agagagacaa agtaatgttt tatggccgga agatcatgag gaaggtgacc acactccccc
      541 ataccttggt tgggaatacc tctgcacccc ggcagcgagt caggaagagg accaaagtgc
      601 tgtctttagc caagaggatt ctgcgcttta agaaagagta tcccactttg cagccaaaag
      661 agcctccgcc ctccctgctt gaggctgatc ttacagagtt tgatgtgaag aactctcatc
      721 tgccatcaga agtcttgtac atgttgaaaa atgttcgggt tctaggccac tttgagaagc
      781 cactatttct ggaactttgc aaacatatgg tctttgtgca gctacaagaa ggggagcacg
      841 tattccaacc tggggagcct gacatcagca tctatgtggt tcaggacgga cggctggagg
      901 tctgcatcca agacgctgat ggcaccgaag tggtggtcaa agaggtcctg ccaggggaca
      961 gtgtccacag tctcctcagc attttggatg tgatcaccgg tcacactgca ccatacaaaa
     1021 cagtttctgc ccgtgccgct gtctcgtcca ccgtgctctg gcttcctgca gcagcttttc
     1081 aaggagtgtt tgagaaatac cctgaaactc tggtcagggt tgtgcagatc atcatggtcc
     1141 ggctgcagcg ggtgactttc ctggctctgc acaactacct ggggctgact acagagctct
     1201 tcaaccctga gagccaggcc atccctctct tgtcagtagc aagtgtggct ggacgagcca
     1261 agagacagat gtcatatggt ccagaggagc agcttgagag gtccctacgg ccctcggagt
     1321 tcagcagctc agatcatggg agcagctgtg tgacagtctc tgggcctctg ctgaagagga
     1381 gctgctcagt gcctctgcct tctaaccatg gggaggtgga tgagctcaga cagtcccagg
     1441 gaagtggctc taacacttct gccttccaag aaagccatga gggagccacc tcagacctgg
     1501 ggatggcata caaccgtgcc aggattttgc cacactctga tgagcaactt gggaactctt
     1561 tggccagcaa gtccaagaaa agtgttgtcg cagagacccc ctctgccatc ttccactact
     1621 cagaaaattt cagggatgag actggggcct gtgggaagac agatgctatc ttcagagctg
     1681 ccacaaagga cctccttacc ctgatgaagc tagatgaccc atccctgttg gatggccggg
     1741 tggcatttct tcatgttcct gcaggcacct tagtgtcaaa acagggagat caggatgtca
     1801 atattttgtt tgtggtctcg gggatgctgc atgtgtacca gcaaaagatt gacagcttgg
     1861 aggatacttg cctgttcctc acacatcccg gggagatggt gggtcagctg gcggtgctca
     1921 ctggagaacc cctaatgttc accatcagag ccaaccgaga ctgcagcttt ttgtccatct
     1981 cgaaggccca cttctatgag atcatgagga aacggccgga tgttgtcctg ggcgtggcac
     2041 acacagttgt gaagaggatg tcatcctttg tacggcagat tgattttgct ctagactgga
     2101 tggaagtaga ggctggacgt gccatataca ggcaaggaga caagtctgac tgcacataca
     2161 tcgttctcag tggcaggctg cgttctgtca tccgaaaaga tgatgggaaa aaacgcctgg
     2221 caggggaata tggccgagga gaccttgttg gtgtggtgga gacgctgacc caccaggcca
     2281 gagcaaccac agtgcatgct gtccgggact cagaactggc taagctgcca gcaggagctc
     2341 tgacgtctat caagcgcagg tacccacagg ttgtaacgcg actgattcat ctcttgggcg
     2401 agaagatctt aggcagtctc cagcagggat ctgcaacagg tcaccagctt ggatttaaca
     2461 cagcaagcag caaatgggac ttggggaacc cgcctggcaa tctgtccaca gtggcagcac
     2521 tgcctgcttc tgaggatgtg cccctaaccg cctttgctct ggagctccaa catgccctca
     2581 gtgccatcgg tcctgtcctg ctgctgacca gtgacaacat caaacaacgc ctgggttccg
     2641 ctgctctaga cagcatccat gagtaccggc tctccagttg gctgggccag caggaggaca
     2701 tccaccggat tgtgctgtat caggcagacg gcacactcac accgtggacc cagcgctgca
     2761 tccggcaggc tgactgcatc ctcatcgtgg gcttgggtga gcaagagcca gcagtaggtg
     2821 agctggagca gatgttggag agcacagctg tgcgtgccca gaagcagcta atcttgctgc
     2881 acaaagagga cggtccagtg ccttcccgca cagtggagtg gctcaacatg cgaagttggt
     2941 gttctggcca tttgcacctc tgctgcccac gccgtgtctt ctccaagaga agcttgccca
     3001 agctggtcga gatgtacaca cgtgttttcc aacgaccacc agaccgtcat tctgattttt
     3061 cccgcctggc acggatgctg acaggcaatg ccattgcctt ggtgcttgga gggggtggag
     3121 ctagagggtg tgcccaggtg ggcatcctca gggcactagc agagtgtggc gtccccgtgg
     3181 acataatcgg aggcacatcc atcggggctt tcatgggtgc tctgtttgct gaggagcgga
     3241 gctacagcca aacacggatc cgggccaagc agtgggcaga gggtatgaca tcaatgatga
     3301 agactatact ggacctgacc tacccaatca catccatgtt ctctggcact ggcttcaata
     3361 gcagcatcag caacatcttc aaagaccggc agattgagga cctgtggctc ccctactttg
     3421 ccatcaccac cgacatcacg gcctctgcca tgcgggttca cacagatggc tctctgtgga
     3481 ggtatgtccg tgcaagcatg tccttgtcag gctatatgcc cccactctgc gaccccaagg
     3541 atggacacct gctgatggat ggtggttaca tcaacaacct cccagcggat gtggcaagat
     3601 ccatgggggc aaaggttgtg attgccatcg atgttggcag ccgggacgag acagacctca
     3661 ccaactatgg cgatgccttg tctggatggt ggctgctttg gaaacgctgg aaccccttgg
     3721 ctacaaaagt caaggtgttg aacatggcag agattcagac tcgactggcc tatgtgtgct
     3781 gcgtccggca gctggagatg gtgaagaaca gcgactactg tgagtacctg aggcctccca
     3841 tcgacagtta ccgcaccctg gacttcggca aatttgatga gatctgtgaa gttgggtatc
     3901 aacatgggcg gacagtgttt gacatctggg tccggagtgg tgtcctagag aagatgttgc
     3961 aggaccagca ggggacaagc aagaggaagg actgtggggt gttcacctgc cccaattcct
     4021 ccttcacgga ccttgctgag attgtgtcac ggattgagcc ggccaaggtg gctgcagtgg
     4081 atgatgagtc tgactatcag accgagtatg aggaggaact gccagccatc cccaaggaaa
     4141 cgtatgctga cttccagagc actgggattg agctagactc tgactcggaa tatgagcctt
     4201 cgatgttgca aggacccccc agcttgactt ccccagaaca gtcccaggac tccttccctt
     4261 ggctccctaa tcaagatgac caggggccaa gactggaaca tccttcctga ctggataaat
     4321 aaataaaaca cctgggcctc acccttagac tgaggctgca cccattcctt agaggctact
     4381 tcagttgcct gacaagatca aactggatga tatagccatc tggcttcttc cagagacatg
     4441 ggctgtgtta ttggagtcgt gggcctcttc acttccccgg cacccttgta tccccacagc
     4501 agtactgagg agcacggacg cctctgtgag gcatgggaag gtgggattgt tgaactaata
     4561 aacgttaatc tgattgtgtg gctattttaa aa
//
