LOCUS       NM_001408339            3727 bp    mRNA    linear   ROD 17-JUN-2024
DEFINITION  Mus musculus patatin-like phospholipase domain containing 3
            (Pnpla3), transcript variant 2, mRNA.
ACCESSION   NM_001408339
VERSION     NM_001408339.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Johnson,S.M., Bao,H., McMahon,C.E., Chen,Y., Burr,S.D.,
            Anderson,A.M., Madeyski-Bengtson,K., Linden,D., Han,X. and Liu,J.
  TITLE     PNPLA3 is a triglyceride lipase that mobilizes polyunsaturated
            fatty acids to facilitate hepatic secretion of large-sized very
            low-density lipoprotein
  JOURNAL   Nat Commun 15 (1), 4847 (2024)
   PUBMED   38844467
  REMARK    GeneRIF: PNPLA3 is a triglyceride lipase that mobilizes
            polyunsaturated fatty acids to facilitate hepatic secretion of
            large-sized very low-density lipoprotein.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Volkert,I., Fromme,M., Schneider,C., Candels,L., Lindhauer,C.,
            Su,H., Thorhauge,K., Pons,M., Mohamed,M.R., Schneider,K.M.,
            Strnad,P. and Trautwein,C.
  TITLE     Impact of PNPLA3 I148M on alpha-1 antitrypsin deficiency-dependent
            liver disease progression
  JOURNAL   Hepatology 79 (4), 898-911 (2024)
   PUBMED   37625151
  REMARK    GeneRIF: Impact of PNPLA3 I148M on alpha-1 antitrypsin
            deficiency-dependent liver disease progression.
REFERENCE   3  (bases 1 to 3727)
  AUTHORS   Su,H., Haque,M., Becker,S., Edlund,K., Duda,J., Wang,Q.,
            Reissing,J., Marschall,H.U., Candels,L.S., Mohamed,M., Sjoland,W.,
            Liao,L., Drexler,S.A., Strowig,T., Rahnenfuhrer,J., Hengstler,J.G.,
            Hatting,M. and Trautwein,C.
  TITLE     Long-term hypercaloric diet exacerbates metabolic liver disease in
            PNPLA3 I148M animals
  JOURNAL   Liver Int 43 (8), 1699-1713 (2023)
   PUBMED   37073116
  REMARK    GeneRIF: Long-term hypercaloric diet exacerbates metabolic liver
            disease in PNPLA3 I148M animals.
REFERENCE   4  (bases 1 to 3727)
  AUTHORS   Patsenker,E., Thangapandi,V.R., Knittelfelder,O., Palladini,A.,
            Hefti,M., Beil-Wagner,J., Rogler,G., Buch,T., Shevchenko,A.,
            Hampe,J. and Stickel,F.
  TITLE     The PNPLA3 variant I148M reveals protective effects toward
            hepatocellular carcinoma in mice via restoration of omega-3
            polyunsaturated fats
  JOURNAL   J Nutr Biochem 108, 109081 (2022)
   PUBMED   35691594
  REMARK    GeneRIF: The PNPLA3 variant I148M reveals protective effects toward
            hepatocellular carcinoma in mice via restoration of omega-3
            polyunsaturated fats.
REFERENCE   5  (bases 1 to 3727)
  AUTHORS   Tardelli,M., Bruschi,F.V., Fuchs,C.D., Claudel,T., Auer,N.,
            Kunczer,V., Ronda,O.A.H.O., Verkade,H.J., Stojakovic,T.,
            Scharnagl,H. and Trauner,M.
  TITLE     Absence of Adiponutrin (PNPLA3) and Monoacylglycerol Lipase
            Synergistically Increases Weight Gain and Aggravates
            Steatohepatitis in Mice
  JOURNAL   Int J Mol Sci 22 (4), 2126 (2021)
   PUBMED   33672787
  REMARK    GeneRIF: Absence of Adiponutrin (PNPLA3) and Monoacylglycerol
            Lipase Synergistically Increases Weight Gain and Aggravates
            Steatohepatitis in Mice.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3727)
  AUTHORS   Basantani,M.K., Sitnick,M.T., Cai,L., Brenner,D.S., Gardner,N.P.,
            Li,J.Z., Schoiswohl,G., Yang,K., Kumari,M., Gross,R.W., Zechner,R.
            and Kershaw,E.E.
  TITLE     Pnpla3/Adiponutrin deficiency in mice does not contribute to fatty
            liver disease or metabolic syndrome
  JOURNAL   J Lipid Res 52 (2), 318-329 (2011)
   PUBMED   21068004
  REMARK    GeneRIF: data argue against a role for Pnpla3 loss-of-function in
            fatty liver disease or metabolic syndrome in mice.
REFERENCE   7  (bases 1 to 3727)
  AUTHORS   Chen,W., Chang,B., Li,L. and Chan,L.
  TITLE     Patatin-like phospholipase domain-containing 3/adiponutrin
            deficiency in mice is not associated with fatty liver disease
  JOURNAL   Hepatology 52 (3), 1134-1142 (2010)
   PUBMED   20648554
  REMARK    GeneRIF: Loss of Pnpla3 does not cause fatty liver, liver enzyme
            elevation, or insulin resistance in mice.
            Erratum:[Hepatology. 2010 Dec;52(6):2250]
REFERENCE   8  (bases 1 to 3727)
  AUTHORS   Hoekstra,M., Li,Z., Kruijt,J.K., Van Eck,M., Van Berkel,T.J. and
            Kuiper,J.
  TITLE     The expression level of non-alcoholic fatty liver disease-related
            gene PNPLA3 in hepatocytes is highly influenced by hepatic lipid
            status
  JOURNAL   J Hepatol 52 (2), 244-251 (2010)
   PUBMED   20015565
  REMARK    GeneRIF: Microarray-based gene profiling showed that the expression
            level of PNPLA3 in hepatocytes is correlated with that of genes
            associated with the lipogenic pathway such as ME1, SPOT14, and
            SCD1.
REFERENCE   9  (bases 1 to 3727)
  AUTHORS   Kershaw,E.E., Hamm,J.K., Verhagen,L.A., Peroni,O., Katic,M. and
            Flier,J.S.
  TITLE     Adipose triglyceride lipase: function, regulation by insulin, and
            comparison with adiponutrin
  JOURNAL   Diabetes 55 (1), 148-157 (2006)
   PUBMED   16380488
  REMARK    GeneRIF: data suggest that murine ATGL but not adiponutrin
            contributes to net adipocyte lipolysis and that ATGL and
            adiponutrin are oppositely regulated by insulin both in vitro and
            in vivo
REFERENCE   10 (bases 1 to 3727)
  AUTHORS   Baulande,S., Lasnier,F., Lucas,M. and Pairault,J.
  TITLE     Adiponutrin, a transmembrane protein corresponding to a novel
            dietary- and obesity-linked mRNA specifically expressed in the
            adipose lineage
  JOURNAL   J Biol Chem 276 (36), 33336-33344 (2001)
   PUBMED   11431482
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL626769.18.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR17784648.1118681.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-245               AL626769.18        43336-43580
            246-478             AL626769.18        46602-46834
            479-544             AL626769.18        48236-48301
            545-754             AL626769.18        49788-49997
            755-815             AL626769.18        52336-52396
            816-971             AL626769.18        54700-54855
            972-1104            AL626769.18        56440-56572
            1105-1209           AL626769.18        61497-61601
            1210-1326           AL626769.18        61923-62039
            1327-3727           AL626769.18        62639-65039
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 39.78 cM"
     gene            1..3727
                     /gene="Pnpla3"
                     /gene_synonym="Adpn"
                     /note="patatin-like phospholipase domain containing 3"
                     /db_xref="GeneID:116939"
                     /db_xref="MGI:MGI:2151796"
     exon            1..245
                     /gene="Pnpla3"
                     /gene_synonym="Adpn"
                     /inference="alignment:Splign:2.1.0"
     CDS             59..1417
                     /gene="Pnpla3"
                     /gene_synonym="Adpn"
                     /EC_number="3.1.1.3"
                     /EC_number="3.1.1.4"
                     /EC_number="2.3.1.51"
                     /note="isoform 2 is encoded by transcript variant 2;
                     patatin-like phospholipase domain-containing protein 3;
                     iPLA2-epsilon; acylglycerol O-acyltransferase;
                     calcium-independent phospholipase A2-epsilon; adiponutrin;
                     acylglycerol transacylase; lysophosphatidic acid
                     acyltransferase; 1-acylglycerol-3-phosphate
                     O-acyltransferase Pnpla3"
                     /codon_start=1
                     /product="1-acylglycerol-3-phosphate O-acyltransferase
                     Pnpla3 isoform 2"
                     /protein_id="NP_001395268.1"
                     /db_xref="GeneID:116939"
                     /db_xref="MGI:MGI:2151796"
                     /translation="MYDPERRWSLSFAGCGFLGFYHVGATLCLSERAPHLLRDARTFF
                     GCSAGALHAVTFVCSLPLGRIMEILMDLVRKARSRNIGTLHPFFNINKCIRDGLQESL
                     PDNVHQVISGKVHISLTRVSDGENVLVSEFHSKDEVVDALVCSCFIPLFSGLIPPSFR
                     GERYVDGGVSDNVPVLDAKTTITVSPFYGEHDICPKVKSTNFFHVNITNLSLRLCTGN
                     LQLLTRALFPSDVKVMGELCYQGYLDAFRFLEENGICNGPQRSLSLSLVAPEACLENG
                     KLVGDKVPVSLCFTDENIWETLSPELSTALSEAIKDREGYLSKVCNLLPVRILSYIML
                     PCSLPVESAIAAVHRLVTWLPDIQDDIQWLQWATSQVCARMTMCLLPSTSMRFLGQSQ
                     FLGLHCIKGLKTKGKAQLSLQHASSARTTARVSLYRPGCPGTHYVSQAGLKLRNPPAS
                     AS"
     exon            246..478
                     /gene="Pnpla3"
                     /gene_synonym="Adpn"
                     /inference="alignment:Splign:2.1.0"
     exon            479..544
                     /gene="Pnpla3"
                     /gene_synonym="Adpn"
                     /inference="alignment:Splign:2.1.0"
     exon            545..754
                     /gene="Pnpla3"
                     /gene_synonym="Adpn"
                     /inference="alignment:Splign:2.1.0"
     exon            755..815
                     /gene="Pnpla3"
                     /gene_synonym="Adpn"
                     /inference="alignment:Splign:2.1.0"
     exon            816..971
                     /gene="Pnpla3"
                     /gene_synonym="Adpn"
                     /inference="alignment:Splign:2.1.0"
     exon            972..1104
                     /gene="Pnpla3"
                     /gene_synonym="Adpn"
                     /inference="alignment:Splign:2.1.0"
     exon            1105..1209
                     /gene="Pnpla3"
                     /gene_synonym="Adpn"
                     /inference="alignment:Splign:2.1.0"
     exon            1210..1326
                     /gene="Pnpla3"
                     /gene_synonym="Adpn"
                     /inference="alignment:Splign:2.1.0"
     exon            1327..3727
                     /gene="Pnpla3"
                     /gene_synonym="Adpn"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3678..3683
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pnpla3"
                     /gene_synonym="Adpn"
                     /note="hexamer: AATAAA"
     regulatory      3691..3696
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pnpla3"
                     /gene_synonym="Adpn"
                     /note="hexamer: AACAAA"
     regulatory      3702..3707
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pnpla3"
                     /gene_synonym="Adpn"
                     /note="hexamer: AATAAA"
     polyA_site      3703
                     /gene="Pnpla3"
                     /gene_synonym="Adpn"
                     /note="major polyA site"
     polyA_site      3707
                     /gene="Pnpla3"
                     /gene_synonym="Adpn"
     polyA_site      3727
                     /gene="Pnpla3"
                     /gene_synonym="Adpn"
                     /note="major polyA site"
ORIGIN      
        1 agagcagcaa caccgggagc agagctgaac tgcagcgccg cccggagctt caagcaccat
       61 gtatgaccca gagcgccgct ggagcctgtc gtttgcaggc tgcggcttcc tgggcttcta
      121 ccacgtcggg gctacgctat gtctgagcga gcgcgccccg cacctcctcc gcgatgcgcg
      181 cactttcttt ggctgctcgg ccggtgcact gcacgcggtc accttcgtgt gcagtctccc
      241 tctcggccgt ataatggaga tcctcatgga cctcgtgcgg aaagccagga gccgcaacat
      301 cggcaccctc cacccgttct tcaacattaa caagtgcatc agagacgggc tccaggagag
      361 cctcccagac aatgtccacc aggtcatttc tggcaaggtt cacatctcac tcaccagggt
      421 gtcggatggg gagaacgtgc tggtgtctga gttccattcc aaagacgaag tcgtggatgc
      481 cctggtgtgt tcctgcttca ttcccctctt ctctggccta atccctcctt ccttccgagg
      541 cgagcggtac gtggacggag gagtgagcga caacgtccct gtgctggatg ccaaaaccac
      601 catcacggtg tcacctttct acggtgagca tgacatctgc cccaaagtca agtccaccaa
      661 cttcttccac gtgaatatca ccaacctcag cctccgcctc tgcactggga acctccaact
      721 tctgaccaga gcgctcttcc cgtctgatgt gaaggtgatg ggagagctgt gctatcaagg
      781 gtacctggac gccttccggt tcctggagga gaatggcatc tgtaacgggc cacagcgcag
      841 cctgagtctg tccttggtgg cgccagaagc ctgcttggaa aatggcaaac ttgtgggaga
      901 caaggtgcca gtcagcctat gctttacaga tgagaacatc tgggagacac tgtcccccga
      961 gctcagcaca gctctgagtg aagcgattaa ggacagggag ggctacctga gcaaagtctg
     1021 caacctcctg cccgtcagga tcctgtccta catcatgctg ccctgcagtc tgcccgtgga
     1081 gtcggctatc gctgcagtcc acaggctggt gacatggctc cctgatatcc aggatgatat
     1141 ccagtggcta caatgggcga catcccaggt ttgtgcccga atgacgatgt gcctgctccc
     1201 ctctaccagc atgagattcc tgggacaatc ccaattcctt ggcctccatt gtatcaaagg
     1261 gctgaaaacc aaagggaagg cacagctgtc tcttcagcat gcctcttctg ccagaaccac
     1321 tgcaagggtt tctctgtatc gccctggctg tcctggaact cactatgtaa gccaggctgg
     1381 cctcaaactc agaaatccgc ctgcctctgc ctcctgagtg ctgggattaa aggcgtgcgc
     1441 aaccatgccc ggtgtggttt tttttttttt ttaattgaca ggtggatgca tctatataat
     1501 ccataacata ttctctctac aggtatctat taggttttgg gtgaggtgtg gagttctagg
     1561 gaactctgag agaaattcct ggggagtaag tggtttatca agttgattgg aggagttttt
     1621 aatgctatgg acagacagac agaaggacaa cagcatagtc ggggctacca gggagttcag
     1681 gccccggcat cggagataga agcaggatgg ggtctttgaa gagattctga gcccacacag
     1741 cagaggaggg actctctctt tagagctttt gaggatgagg gaggttgact gcaagagcct
     1801 acagccaggc tcgaggcagg cagggggtgg ggagcaggat gtaaacccct tcgatgctga
     1861 cagactcact tctggggtaa aatattatga gatgcctgtc agtgtctgtg aagagacctg
     1921 agcagagtct ggattctgac atcaatcatg ttcttacaat actgaagacc tgagagcctg
     1981 caatcttggt ttgtaaattg ctggtctccg tgcttccagt gaacttggac attcttctca
     2041 tggttggtcc aggagaggcc aaagctgagg gcaccctgcc ttccaccccc agtccagctt
     2101 gaccttttat ctggagcaac agtgtctaga tgatgggtgg gtgaggggtg ctatactgtc
     2161 tgtccctctg ggaagggttc tgttactttt ggaggcagct aggaagtttc tctgtgcagc
     2221 tgccccctgg tgctgtgtgg tgacctcatt gcctgtgacc ccaggatcac aggatctggg
     2281 ctaaagtggt agtccataga aaccaaagac aatgatttgg tgtttagaaa gctactcttg
     2341 gtctgggtga agtctggtgc ttaagggcta tcacaaagag cgtgtcaaac catctctcag
     2401 cctgtgagtc agtggggagc ccaagggcat cagtgtttgg aaactggaat ccaaaccggg
     2461 caatctcgga aggaaactgt ttaggaattg tgatgggacg ggccgtggct gtctctgaaa
     2521 agggcctgcc agataactta ttacttttaa ggacaccttt ggctcttact aatttataaa
     2581 gcattttata taaacacacc agggagtgca tggtgaacta cacgtatgat cagttaagtg
     2641 gggctagaat taggtaggga gagcatcgga cctctgcctc ctcaacctca acttgcttgc
     2701 tttctccact ggctccaaat ctttgtatag tcatcagcca tgaccacctc tctccctccc
     2761 catctactac cagcagcgtt aatgggaata agtacccact tctctcaggt gtactataca
     2821 gctgtgggtg tggtgtgtgt ttcctgtaat tcacacttta gaaaggaaac aagcaaacaa
     2881 aagaaaccag gtgctgccca tactcctaag tgtagacagt gaaggtgtgt gtctcccatg
     2941 cctgagtctc ctggaggcct agtgagctcc aggttcatgc aagcacatca ggaggaatca
     3001 tataatctca gcacggttga tccagatggg ataagaaagg actctgggag agagaatgtg
     3061 gttctagaga caaagtgtct aggctacaca gaagataaga ctgtcccaag gaaagaaaag
     3121 aaaccaggaa ctagggtgca gctcagttgt cagaggactt ctctaggctt gaagcccaga
     3181 gtccaatctc agcaccttat aaactgtgga gtgacaggca gtgacatcgg cctgtaatcc
     3241 caacactcaa gcagtagagg caagaggatc ataagttcaa ggtcttcctt ggctatttag
     3301 ggagttggag gttagctctg gctacatgag accctgtctc aaaaaaaaaa aaaaaaaaaa
     3361 gtagaaactt ctgccttgct ttgagctgcc cctttctgga cgtttctcat cagtagagaa
     3421 tattcctgcc accctatcag acaaaactcc cactggtttg gagtctctcc attctcagga
     3481 acacctcagg agtcagacag tgagcagcag ggagcaatgt cttgacttgt aagcccctta
     3541 gcaaggctgg ttcatttgtt tattaaaagc aggtgtgggt gaatttatgc aaatgagtat
     3601 gcaaactagt ggaacagcag aaggattgaa tggatacacc aaaaataacc acaactgttt
     3661 aagggaaaag ggtccataat aaatgtgggg aacaaaaaac aaataaatgt gatttttttt
     3721 agaaaaa
//
