LOCUS       NM_013632               2941 bp    mRNA    linear   ROD 31-JUL-2024
DEFINITION  Mus musculus purine-nucleoside phosphorylase (Pnp), mRNA.
ACCESSION   NM_013632 XM_907682
VERSION     NM_013632.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Zocher,S., McCloskey,A., Karasinsky,A., Schulte,R., Friedrich,U.,
            Lesche,M., Rund,N., Gage,F.H., Hetzer,M.W. and Toda,T.
  TITLE     Lifelong persistence of nuclear RNAs in the mouse brain
  JOURNAL   Science 384 (6691), 53-59 (2024)
   PUBMED   38574132
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2941)
  AUTHORS   Shimada,E., Ahsan,F.M., Nili,M., Huang,D., Atamdede,S., TeSlaa,T.,
            Case,D., Yu,X., Gregory,B.D., Perrin,B.J., Koehler,C.M. and
            Teitell,M.A.
  TITLE     PNPase knockout results in mtDNA loss and an altered metabolic gene
            expression program
  JOURNAL   PLoS One 13 (7), e0200925 (2018)
   PUBMED   30024931
  REMARK    GeneRIF: Loss of PNP expression is associated with hearing loss.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2941)
  AUTHORS   Choo,H.J., Cutler,A., Rother,F., Bader,M. and Pavlath,G.K.
  TITLE     Karyopherin Alpha 1 Regulates Satellite Cell Proliferation and
            Survival by Modulating Nuclear Import
  JOURNAL   Stem Cells 34 (11), 2784-2797 (2016)
   PUBMED   27434733
REFERENCE   5  (bases 1 to 2941)
  AUTHORS   Mansouri,A., Min,W., Cole,C.J., Josselyn,S.A., Henderson,J.T., van
            Eede,M., Henkelman,R.M., Ackerley,C., Grunebaum,E. and Roifman,C.M.
  TITLE     Cerebellar abnormalities in purine nucleoside phosphorylase
            deficient mice
  JOURNAL   Neurobiol Dis 47 (2), 201-209 (2012)
   PUBMED   22521465
  REMARK    GeneRIF: PNP deficiency causes cerebellar abnormalities, including
            Purkinje cell damage and progressive motor deficits.
REFERENCE   6  (bases 1 to 2941)
  AUTHORS   Bremner,T.A., Premkumar-Reddy,E., Nayar,K. and Kouri,R.E.
  TITLE     Nucleoside phosphorylase 2 (Np-2) of mice
  JOURNAL   Biochem Genet 16 (11-12), 1143-1151 (1978)
   PUBMED   109081
REFERENCE   7  (bases 1 to 2941)
  AUTHORS   Womack,J.E., Davisson,M.T., Eicher,E.M. and Kendall,D.A.
  TITLE     Mapping of nucleoside phosphorylase (Np-1) and esterase 10 (Es-10)
            on mouse chromosome 14
  JOURNAL   Biochem Genet 15 (3-4), 347-355 (1977)
   PUBMED   405970
REFERENCE   8  (bases 1 to 2941)
  AUTHORS   Kozak,C.A. and Ruddle,F.H.
  TITLE     Assignment of the genes for thymidine kinase and galactokinase to
            Mus musculus chromosome 11 and the preferential segregation of this
            chromosome in Chinese hamster/mouse somatic cell hybrids
  JOURNAL   Somatic Cell Genet 3 (2), 121-133 (1977)
   PUBMED   204065
REFERENCE   9  (bases 1 to 2941)
  AUTHORS   Francke,U., Lalley,P.A., Moss,W., Ivy,J. and Minna,J.D.
  TITLE     Gene mapping in Mus musculus by interspecific cell hybridization:
            assignment of the genes for tripeptidase-1 to chromosome 10,
            dipeptidase-2 to chromosome 18, acid phosphatase-1 to chromosome
            12, and adenylate kinase-1 to chromosome 2
  JOURNAL   Cytogenet Cell Genet 19 (2-3), 57-84 (1977)
   PUBMED   198184
REFERENCE   10 (bases 1 to 2941)
  AUTHORS   Womack,J.E. and Sharp,M.
  TITLE     Comparative autosomal linkage in mammals: genetics of esterases in
            Mus musculus and Rattus norvegicus
  JOURNAL   Genetics 82 (4), 665-675 (1976)
   PUBMED   57899
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK153451.1, AK049817.1 and BB613747.1.
            
            [WARNING] On Feb 13, 2025 this sequence was replaced by
            NM_013632.5.
            
            On May 15, 2009 this sequence version replaced NM_013632.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK049817.1, AK153451.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-222               AK153451.1         3-224
            223-1824            AK049817.1         2-1603
            1825-1833           BB613747.1         464-472
            1834-2941           AK049817.1         1611-2718
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 26.31 cM"
     gene            1..2941
                     /gene="Pnp"
                     /gene_synonym="Np; Np-1; Np-2; Pnp1"
                     /note="purine-nucleoside phosphorylase"
                     /db_xref="GeneID:18950"
                     /db_xref="MGI:MGI:97365"
     exon            1..291
                     /gene="Pnp"
                     /gene_synonym="Np; Np-1; Np-2; Pnp1"
                     /inference="alignment:Splign:2.1.0"
     CDS             281..1150
                     /gene="Pnp"
                     /gene_synonym="Np; Np-1; Np-2; Pnp1"
                     /EC_number="2.4.2.1"
                     /note="inosine phosphorylase; purine-nucleoside
                     phosphorylase 1; inosine-guanosine phosphorylase"
                     /codon_start=1
                     /product="purine nucleoside phosphorylase"
                     /protein_id="NP_038660.1"
                     /db_xref="CCDS:CCDS27029.1"
                     /db_xref="GeneID:18950"
                     /db_xref="MGI:MGI:97365"
                     /translation="MENEFTYEDYETTAKWLLQHTEYRPQVAVICGSGLGGLTAHLKE
                     AQIFDYNEIPNFPQSTVQGHAGRLVFGLLNGRCCVMMQGRFHMYEGYSLSKVTFPVRV
                     FHLLGVETLVVTNAAGGLNPNFEVGDIMLIRDHINLPGFCGQNPLRGPNDERFGVRFP
                     AMSDAYDRDMRQKAFSAWKQMGEQRKLQEGTYVMLAGPNFETVAESRLLKMLGADAVG
                     MSTVPEVIVARHCGLRVFGFSLITNKVVMDYENLEKANHMEVLDAGKAAAQTLERFVS
                     ILMESIPLPDRGS"
     exon            292..461
                     /gene="Pnp"
                     /gene_synonym="Np; Np-1; Np-2; Pnp1"
                     /inference="alignment:Splign:2.1.0"
     exon            462..565
                     /gene="Pnp"
                     /gene_synonym="Np; Np-1; Np-2; Pnp1"
                     /inference="alignment:Splign:2.1.0"
     exon            566..741
                     /gene="Pnp"
                     /gene_synonym="Np; Np-1; Np-2; Pnp1"
                     /inference="alignment:Splign:2.1.0"
     exon            742..932
                     /gene="Pnp"
                     /gene_synonym="Np; Np-1; Np-2; Pnp1"
                     /inference="alignment:Splign:2.1.0"
     exon            933..2941
                     /gene="Pnp"
                     /gene_synonym="Np; Np-1; Np-2; Pnp1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1520..1525
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pnp"
                     /gene_synonym="Np; Np-1; Np-2; Pnp1"
                     /note="hexamer: AATAAA"
     polyA_site      1541
                     /gene="Pnp"
                     /gene_synonym="Np; Np-1; Np-2; Pnp1"
                     /note="major polyA site"
ORIGIN      
        1 ttttctggct gggaaatcat gactgtgtgc gggtttcatc tgctggttgc cttagactag
       61 gagtgggtct ctttgaaaag aactctggag aggtttcctg ccacacctct ggatgagggt
      121 gtgtcaccat ggagacaagg ccgctgccat tggctggagc ctggggctga ggatataaag
      181 gccggcccag cgaatagtgc actaacagtc ttgacctgac tcagacagtg tcgccgtgga
      241 gcggaaaggg caggatttcg gcgtggccgt cggtgactcc atggagaacg agttcacata
      301 cgaagattat gagaccactg ccaagtggct tctgcaacac actgaatatc gacctcaagt
      361 ggcagtgatc tgtggttccg gcttaggagg gctgactgct cacttaaagg aggctcagat
      421 ctttgactac aatgagatac ccaactttcc ccaaagcaca gtgcaaggtc acgcaggccg
      481 actggtgttt ggattgctga atggcagatg ctgtgtgatg atgcaaggcc ggttccatat
      541 gtatgaagga tactcactgt caaaggtgac attcccagtg agagttttcc atcttctggg
      601 tgtggaaact ttggtggtca ccaatgctgc tggaggactc aaccccaatt ttgaagttgg
      661 agatattatg ctgatccgtg atcacatcaa cctacctggt ttctgtggcc agaaccctct
      721 ccggggcccc aacgatgaaa ggtttggagt tcgttttcct gccatgtctg atgcttatga
      781 ccgggatatg aggcagaagg ctttcagtgc ctggaaacaa atgggggagc aacgaaagct
      841 acaagaaggc acctacgtga tgttggcagg ccccaacttt gagactgtgg cagagagtcg
      901 tctgctaaag atgctggggg cagatgctgt tggcatgagc acagtcccag aagttatcgt
      961 cgcaaggcac tgtgggctcc gtgtctttgg tttctcactc attacgaaca aggttgtcat
     1021 ggattatgag aacttggaga aggccaatca catggaagtt ctggatgccg ggaaagcagc
     1081 tgcacagaca ttggaaaggt ttgtctccat tcttatggag agcattccac tcccggatcg
     1141 tggcagctga cctgcaatgg agtgctccgc tgctcccctg gtggggtcca agtagctctc
     1201 tactctgttt ccttgatgaa gtcactagtc tctgttctta gatcctagca gaaaggaaag
     1261 aggagcttac ccttcacttt ccagatgttg ttctactgtt ctaatgccca gtcttcttgc
     1321 ttagttgcct tctcaaatta actttcattc ctctggaaga gctggagccc attcctccca
     1381 cacttgagat atatgccttc aaacagtacc ttctacatct tggagatgac agacatgttc
     1441 tgggctcagt ctaaagccct aaggaccaaa gatcaaccca atacctcttg gattttattt
     1501 agtatcacaa tgttttgaga ataaagagat cgagtacttt tcttttgtgt gtggttcggt
     1561 atgaagctgg ggcatcggcc aaaaacgact tgaaaagctt tggggaccac atgttccaaa
     1621 agcagctaag aatggagatg agttggtgcg tgagtagcaa gggatgaagg ggaaaaaatg
     1681 gtgtgtgtgt ggaggggatg cgactacttc tttcagactt gttcctagcc ttcatttccc
     1741 acgttctcca tttactccat tgcttgagta tctgcatctc acagatctca cttctccagc
     1801 actgcctttt tttttttttt ttttttcgca ttgttttttt ttctctgctc taatttctga
     1861 gtcatgctct ttccagatct atattgacag tgcagcagca gatacacttt tgtctgccgc
     1921 catttgtcat ggtgatggct acattcattc caactccaac caagcctgta aagttggttc
     1981 acactccttc tctgcttaaa caccatggaa tggctattct tggttatcaa cttgactatg
     2041 attggaatta aaaaaaaaaa caaacaactc aaatagaggg cataactgag ggacttttgc
     2101 ttcatttgaa atggaaagat ctacttttaa tctgaatctt tgaggtagga agacacatct
     2161 ttaatctcaa tcttgagttg ggaatattca ccttcgatcc agctctaatc tggcctgtgc
     2221 ctgctgatgg gaggctatat aaggaagaac atggaggaag gaaactgctc ttcatgtgct
     2281 tactcttgtg cttgtgctgc tagcaagtcc atcccttcac tggcatcaca gcctgcttca
     2341 ggattctagc atatactgca caacgtccaa tggagacagc caacctcagg aacccaaagt
     2401 tcaaatcatt tagaagtgaa aatacaccct agagacgaca tgaaaattat gagggtagtg
     2461 acttctgtac aatatctgcc ttccagcctc atccagtaat aagccatgcc aacatcctgt
     2521 gctaaggtga gagataaaca cttgaacaag actgcagcta gtcaagcaac gtactcaagt
     2581 gtgcgtcacc cacttacagc ataggtacat cagcaagatt tgccccagct agtctgttat
     2641 ggaagataag ctttgccatc ttgtctgtct ccagaatgat tccatggcta cgatatttcc
     2701 aaactcttgc tgggacccaa catttaaggg ggaaatgtgg gtgagaggaa agtggagtac
     2761 tctcagctaa cacacagtct tttttttttt tatataccaa tcccacattg tgatgttcag
     2821 ttttcactcc aactgaactt ttgcataaat tcataacatt ttcttgtttc tgttttccag
     2881 cactttacat atttgagtgt ttaaggagct gggaatagat cagtcagtaa agtgcctacc
     2941 t
//
