LOCUS       NM_001205075            2101 bp    mRNA    linear   ROD 06-AUG-2023
DEFINITION  Mus musculus prepronociceptin (Pnoc), transcript variant 2, mRNA.
ACCESSION   NM_001205075
VERSION     NM_001205075.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Liau ES, Jin S, Chen YC, Liu WS, Calon M, Nedelec S, Nie Q and Chen
            JA.
  TITLE     Single-cell transcriptomic analysis reveals diversity within
            mammalian spinal motor neurons
  JOURNAL   Nat Commun 14 (1), 46 (2023)
   PUBMED   36596814
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Cao R, Takechi M, Wang X, Furutera T, Nojiri T, Koyabu D and Li J.
  TITLE     Temporal and regulatory dynamics of the inner ear transcriptome
            during development in mice
  JOURNAL   Sci Rep 12 (1), 21196 (2022)
   PUBMED   36476755
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2101)
  AUTHORS   Roome RB, Bourojeni FB, Mona B, Rastegar-Pouyani S, Blain R,
            Dumouchel A, Salesse C, Thompson WS, Brookbank M, Gitton Y,
            Tessarollo L, Goulding M, Johnson JE, Kmita M, Chedotal A and Kania
            A.
  TITLE     Phox2a Defines a Developmental Origin of the Anterolateral System
            in Mice and Humans
  JOURNAL   Cell Rep 33 (8), 108425 (2020)
   PUBMED   33238113
REFERENCE   4  (bases 1 to 2101)
  AUTHORS   Rodriguez-Romaguera J, Ung RL, Nomura H, Otis JM, Basiri ML,
            Namboodiri VMK, Zhu X, Robinson JE, van den Munkhof HE, McHenry JA,
            Eckman LEH, Kosyk O, Jhou TC, Kash TL, Bruchas MR and Stuber GD.
  TITLE     Prepronociceptin-Expressing Neurons in the Extended Amygdala Encode
            and Promote Rapid Arousal Responses to Motivationally Salient
            Stimuli
  JOURNAL   Cell Rep 33 (6), 108362 (2020)
   PUBMED   33176134
REFERENCE   5  (bases 1 to 2101)
  AUTHORS   Montiel JL, Cornille F, Roques BP and Noble F.
  TITLE     Nociceptin/orphanin FQ metabolism: role of aminopeptidase and
            endopeptidase 24.15
  JOURNAL   J Neurochem 68 (1), 354-361 (1997)
   PUBMED   8978746
REFERENCE   6  (bases 1 to 2101)
  AUTHORS   Mollereau C, Simons MJ, Soularue P, Liners F, Vassart G, Meunier JC
            and Parmentier M.
  TITLE     Structure, tissue distribution, and chromosomal localization of the
            prepronociceptin gene
  JOURNAL   Proc Natl Acad Sci U S A 93 (16), 8666-8670 (1996)
   PUBMED   8710928
REFERENCE   7  (bases 1 to 2101)
  AUTHORS   Saito Y, Maruyama K, Kawano H, Hagino-Yamagishi K, Kawamura K,
            Saido TC and Kawashima S.
  TITLE     Molecular cloning and characterization of a novel form of
            neuropeptide gene as a developmentally regulated molecule
  JOURNAL   J Biol Chem 271 (26), 15615-15622 (1996)
   PUBMED   8663129
REFERENCE   8  (bases 1 to 2101)
  AUTHORS   Pan YX, Xu J and Pasternak GW.
  TITLE     Cloning and expression of a cDNA encoding a mouse brain orphanin
            FQ/nociceptin precursor
  JOURNAL   Biochem J 315 (Pt 1) (Pt 1), 11-13 (1996)
   PUBMED   8670093
REFERENCE   9  (bases 1 to 2101)
  AUTHORS   Houtani T, Nishi M, Takeshima H, Nukada T and Sugimoto T.
  TITLE     Structure and regional distribution of nociceptin/orphanin FQ
            precursor
  JOURNAL   Biochem Biophys Res Commun 219 (3), 714-719 (1996)
   PUBMED   8645247
REFERENCE   10 (bases 1 to 2101)
  AUTHORS   Saito Y, Maruyama K, Saido TC and Kawashima S.
  TITLE     N23K, a gene transiently up-regulated during neural
            differentiation, encodes a precursor protein for a newly identified
            neuropeptide nociceptin
  JOURNAL   Biochem Biophys Res Commun 217 (2), 539-545 (1995)
   PUBMED   7503733
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC158983.2 and AK045893.1.
            
            [WARNING] On Sep 26, 2023 this sequence was replaced by
            NM_001205075.2.
            
            Summary: This gene encodes the precursor for neuropeptides that
            have been implicated in a wide range of physiological roles such as
            transmission and sensitivity to pain, learning, memory, anxiety and
            depression, in the central nervous system. The encoded protein is a
            precursor that is proteolytically processed to generate multiple
            biologically active peptides including nociceptin and nocistatin
            which have opposite functions in pain transmission. Mice lacking
            the encoded protein display increased anxiety, elevated basal pain
            threshold and impaired adaptation to repeated stress. Alternative
            splicing results in multiple transcript variants encoding different
            isoforms. [provided by RefSeq, Jul 2015].
            
            Transcript Variant: This variant (2) uses an alternate donor splice
            site at the penultimate exon compared to variant 1. This results in
            a frameshift, and a longer isoform (2, also known as N27K) with a
            distinct C-terminus, compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC144865.1, D50055.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849376 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-263               AC158983.2         108976-109238
            264-912             AK045893.1         1-649
            913-2101            AK045893.1         706-1894
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 34.33 cM"
     gene            1..2101
                     /gene="Pnoc"
                     /gene_synonym="N/OFQ; Npnc1; OFQ/N"
                     /note="prepronociceptin"
                     /db_xref="GeneID:18155"
                     /db_xref="MGI:MGI:105308"
     exon            1..335
                     /gene="Pnoc"
                     /gene_synonym="N/OFQ; Npnc1; OFQ/N"
                     /inference="alignment:Splign:2.1.0"
     exon            336..485
                     /gene="Pnoc"
                     /gene_synonym="N/OFQ; Npnc1; OFQ/N"
                     /inference="alignment:Splign:2.1.0"
     CDS             360..998
                     /gene="Pnoc"
                     /gene_synonym="N/OFQ; Npnc1; OFQ/N"
                     /note="isoform 2 preproprotein is encoded by transcript
                     variant 2; neuropeptide nociceptin 1; proorphanin;
                     orphanin FQ"
                     /codon_start=1
                     /product="prepronociceptin isoform 2 preproprotein"
                     /protein_id="NP_001192004.1"
                     /db_xref="CCDS:CCDS88690.1"
                     /db_xref="GeneID:18155"
                     /db_xref="MGI:MGI:105308"
                     /translation="MKILFCDVLLLSLLSSVFSSCPRDCLTCQEKLHPAPDSFNLKTC
                     ILQCEEKVFPRPLWTVCTKVMASGSGQLSPADPELVSAALYQPKASEMQHLKRMPRVR
                     SLVQVRDAEPGADAEPGADAEPGADDAEEVEQKQLQKRFGGFTGARKSARKLANQKRF
                     SEFMRQYLVLSMQSSQRRRTLHQNGIQVIPRTACVHSKTCRPGVRIPPSPRH"
     exon            486..912
                     /gene="Pnoc"
                     /gene_synonym="N/OFQ; Npnc1; OFQ/N"
                     /inference="alignment:Splign:2.1.0"
     exon            913..2101
                     /gene="Pnoc"
                     /gene_synonym="N/OFQ; Npnc1; OFQ/N"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 taggctggcc atcctcacag tggctctgaa agcagccgga gaggcagctg agggatccta
       61 gctgatcggg agtccaggtg aaaacaggct agaggctgcg ttgatgggag gctggcatgt
      121 cacaagtctg aacgcggagc aggggagggg gccaggcagg caggattgta gttgggctgt
      181 ttggttgatg aagggggtca gcgtaggcat gtgtcatcat cgaggtaggc agggaagggg
      241 agggctgtgc gtggggggag gatatatatg ctggtgtggc tgacacagag gaacaagcct
      301 cacatctgag agaaccctga aacggacagc gtcagcctcc cgattcccac tgctgcacca
      361 tgaaaatcct cttttgtgac gttctgctgc tcagcctgct ctccagcgtg ttcagcagct
      421 gtcccaggga ctgcctcacc tgccaggaga agctccaccc agctccagac agcttcaact
      481 taaagacgtg catcctccag tgtgaagaga aggtcttccc ccgccctctc tggactgtat
      541 gcaccaaagt catggccagt ggctccgggc agctcagccc tgctgaccca gagcttgtgt
      601 cagctgctct ttaccagcca aaggcctcgg agatgcagca cctgaagaga atgccgcgtg
      661 tccggagctt ggtgcaagtg cgagatgcag agcctggcgc agatgctgag cctggcgcag
      721 atgctgagcc tggcgcagat gacgctgagg aggtggagca gaagcagctg cagaaaaggt
      781 ttgggggctt caccggggcc cggaaatcag cccggaagtt ggccaaccag aagcggttca
      841 gtgagtttat gaggcagtac ctggtcctga gcatgcagtc aagtcaacgc cggcgcaccc
      901 tgcaccagaa tggcatccag gtgatccccc gtacagcatg tgtccactcc aagacctgca
      961 ggccgggagt caggattcct ccctccccga ggcactgagc acccacagca cctccctgcc
     1021 cggttgttgc ccctccctca tggcatgtct caccatgatc ctgttgctac atcagagtgt
     1081 atttttgtaa tttctccagc taacatttta atggccccat cttctcactc atcttctgcc
     1141 ctctcatagg gccaggtgag aggaacatga aatcagacct ggggttttgc ctcttcactg
     1201 ccataacttg tttgtaaaag agctgttctt tttgactgat cgtttaaaac aactttctcc
     1261 attaaacttc tactgagcaa atggttaata aacttgctgc cttttttttt taagatttat
     1321 ttatttattt aacgtacgtg agtatgctgt cttcaaacac gtatcggatc ccattgcaga
     1381 tgattgtgag ccacgatgtg gttgctggga attgaactca ggacctttgg aagagcagtt
     1441 agtgttctta acctctgagc catcttccca gttgcctgat tgtttttttt ttttttttta
     1501 agcgatgcca taagaggcat agactctgca agcacacact ttgagagcca catctcccag
     1561 agctggaaac tcataggctg ctatccataa ctataaagtt ggcaaaagga gatgctggga
     1621 cacgaacata ctgacggcaa ccggcactat aaagccagat gtaaatggca gtgtgcgcct
     1681 atgagcaagg gctggagagg agactgggtc tcaacctttg aggacatcca gctactatta
     1741 gaggccactc gaagctccag acccagcaga atcttataca ttttagcagc ttccaggtga
     1801 tggggctggc aggttgggcc agaggaagaa tggcaagcct gcccacctgc ttttttctcc
     1861 atgtaacact aaactcagtt agctgtgtaa aaatccagcc aggaagcata tggagactcc
     1921 aattggagta gctgaccagt tcaatatcga ctgctgtcac agtccatttt ttttgctgct
     1981 aagacagcag gctacaactg tttttctata tcctagagga aatggttcca gaacccctgt
     2041 gggcagcaaa atcatccact gcacaaagaa tacacttaaa tataacctac atatgagctt
     2101 c
//
