LOCUS       NM_001419440            5491 bp    mRNA    linear   ROD 14-JUN-2024
DEFINITION  Mus musculus peptidase M20 domain containing 2 (Pm20d2), transcript
            variant 1, mRNA.
ACCESSION   NM_001419440 XM_006537900
VERSION     NM_001419440.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5491)
  AUTHORS   Veiga-da-Cunha,M., Chevalier,N., Stroobant,V., Vertommen,D. and Van
            Schaftingen,E.
  TITLE     Metabolite proofreading in carnosine and homocarnosine synthesis:
            molecular identification of PM20D2 as beta-alanyl-lysine
            dipeptidase
  JOURNAL   J Biol Chem 289 (28), 19726-19736 (2014)
   PUBMED   24891507
  REMARK    GeneRIF: PM20D2 hydrolyzed beta-alanyl-lysine,
            beta-alanyl-ornithine, gamma-aminobutyryl-lysine, and
            gamma-aminobutyryl-ornithine as its best substrates.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL670464.12.
            
            On Mar 31, 2023 this sequence version replaced XM_006537900.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR10223970.6122.1,
                                           SRR10223971.4592.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-490               AL670464.12        59856-60345
            491-639             AL670464.12        62080-62228
            640-777             AL670464.12        65458-65595
            778-920             AL670464.12        66057-66199
            921-1075            AL670464.12        67417-67571
            1076-1211           AL670464.12        69936-70071
            1212-1319           AL670464.12        74425-74532
            1320-5491           AL670464.12        74796-78967
FEATURES             Location/Qualifiers
     source          1..5491
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 14.71 cM"
     gene            1..5491
                     /gene="Pm20d2"
                     /gene_synonym="Acy1l2; Gm424"
                     /note="peptidase M20 domain containing 2"
                     /db_xref="GeneID:242377"
                     /db_xref="MGI:MGI:2685270"
     exon            1..490
                     /gene="Pm20d2"
                     /gene_synonym="Acy1l2; Gm424"
                     /inference="alignment:Splign:2.1.0"
     CDS             41..1474
                     /gene="Pm20d2"
                     /gene_synonym="Acy1l2; Gm424"
                     /EC_number="3.4.13.4"
                     /note="isoform 1 is encoded by transcript variant 1;
                     peptidase M20 domain-containing protein 2;
                     aminoacylase-1-like protein 2; aminoacylase 1-like 2;
                     xaa-Arg dipeptidase"
                     /codon_start=1
                     /product="xaa-Arg dipeptidase isoform 1"
                     /protein_id="NP_001406369.1"
                     /db_xref="GeneID:242377"
                     /db_xref="MGI:MGI:2685270"
                     /translation="MGPVVERPAEPGTSSAAELELLKRRAAERIDEAAERLGALSRAI
                     WSAPELAYEEHRAHGELTRFFECEPPAASWAVQPHFGLPTAFRAEWAPPESAAGPRAL
                     QVAFLCEYDALPALGHACGHNLIAEVGVAAALGLRAALESIAAPPPVKVIVLGTPAEE
                     DGGGKIDLIEAGAFENLDVVFMAHPSQENAAYLPDVAEHENGRQSLPHGKQTLPGNYI
                     LVPTSLCFRYRCFSNTQSNMGADRWLCSVTVKYYGKASHAAAYPWEGVNALDAAVLAY
                     TNLSVLRQQMKPTWRVHGIIKNGGVKPNIIPSYSELVYYFRAPSMKELQVLTKKAEDC
                     FRAAALATGCTVDIESEAHDYYNVIPNKTLCSAYTENGKKLGMEFISEDAVLNGPSGS
                     TDFGNVSFVVPGIHPYFYIGTDALNHTEQYTEAAGSQAAQLYTLRTAKALAMTALDVI
                     FKPALLEGVRKEFKCKLQEEQLLNTAA"
     exon            491..639
                     /gene="Pm20d2"
                     /gene_synonym="Acy1l2; Gm424"
                     /inference="alignment:Splign:2.1.0"
     exon            640..777
                     /gene="Pm20d2"
                     /gene_synonym="Acy1l2; Gm424"
                     /inference="alignment:Splign:2.1.0"
     exon            778..920
                     /gene="Pm20d2"
                     /gene_synonym="Acy1l2; Gm424"
                     /inference="alignment:Splign:2.1.0"
     exon            921..1075
                     /gene="Pm20d2"
                     /gene_synonym="Acy1l2; Gm424"
                     /inference="alignment:Splign:2.1.0"
     exon            1076..1211
                     /gene="Pm20d2"
                     /gene_synonym="Acy1l2; Gm424"
                     /inference="alignment:Splign:2.1.0"
     exon            1212..1319
                     /gene="Pm20d2"
                     /gene_synonym="Acy1l2; Gm424"
                     /inference="alignment:Splign:2.1.0"
     exon            1320..5491
                     /gene="Pm20d2"
                     /gene_synonym="Acy1l2; Gm424"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1877..1882
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pm20d2"
                     /gene_synonym="Acy1l2; Gm424"
                     /note="hexamer: AATAAA"
     polyA_site      1921
                     /gene="Pm20d2"
                     /gene_synonym="Acy1l2; Gm424"
                     /note="major polyA site"
     regulatory      5471..5476
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pm20d2"
                     /gene_synonym="Acy1l2; Gm424"
                     /note="hexamer: AATAAA"
     polyA_site      5491
                     /gene="Pm20d2"
                     /gene_synonym="Acy1l2; Gm424"
                     /note="major polyA site"
ORIGIN      
        1 gcagagggcg gacgaggaga gaccgcagca cgcccgcgcc atgggtccgg tggtggagcg
       61 gccggccgag cccggcacga gcagcgccgc cgagctggag ctgctgaaac ggcgagcggc
      121 cgagcgcatc gacgaggcgg ccgagcgcct gggcgcgctg agccgcgcca tctggagcgc
      181 gcccgagctg gcctacgaag agcaccgcgc gcacggcgag ctcacgcgct tcttcgagtg
      241 cgagccgccg gccgcctcgt gggcggtgca gccgcacttc gggctcccca ccgccttccg
      301 tgccgagtgg gcgccgccgg agtccgcggc ggggccccgc gcgctgcagg tggccttcct
      361 gtgcgagtac gacgcgctgc cagccctggg ccacgcctgc ggccacaacc tcatcgccga
      421 ggtcggggtg gctgcggcgc tcggcctgcg ggctgcgctg gagagcatcg ctgcgccgcc
      481 gccggtcaag gtgattgtcc tgggaacccc tgcagaagaa gatggtggtg gcaaaattga
      541 tctaattgaa gcaggtgcct tcgaaaacct tgatgttgtt tttatggctc atccctctca
      601 ggagaacgct gcctatctcc cggatgtggc cgagcacgaa aatggaagac agagccttcc
      661 acatggtaaa cagacactcc ctgggaacta cattctcgtt cccacatccc tatgctttcg
      721 ctacagatgc ttttcaaata cacagtccaa catgggggct gacagatggc tctgtagtgt
      781 gactgtgaag tactacggaa aagcgtctca cgctgccgct tacccttggg agggtgtgaa
      841 cgccttggac gctgcagttc ttgcctatac caacctctct gtgttaagac agcagatgaa
      901 accaacctgg agagttcatg gtataataaa aaacggtggt gtgaagccca atatcatccc
      961 ctcttactct gaactcgtct attacttccg tgcgccctcc atgaaggagc ttcaagttct
     1021 gaccaaaaag gcagaagatt gcttcagagc tgcggctttg gctacgggct gcacagtaga
     1081 cattgaaagt gaagcacatg attattacaa tgtcattccc aataagaccc tgtgcagtgc
     1141 gtatacggag aacgggaaga aactgggaat ggagttcatt tcagaagatg cggtactgaa
     1201 tggcccttca ggctctactg attttggaaa tgtcagtttc gtggttcctg ggattcaccc
     1261 gtatttttac attgggactg atgccttgaa tcacacggag cagtacacag aagctgcagg
     1321 atcacaagcg gctcagctgt acacgctgcg tacagccaag gctctggcaa tgaccgcact
     1381 ggacgtcatc tttaagcctg cattgctcga aggagtcagg aaggagttta aatgtaaact
     1441 tcaagaagag cagcttttaa atacagcagc atagaaggaa accctaggga ctggtcagga
     1501 aatgttacgt tttgtttgtt ttttacctct catgtttttc agtgaaagag agcatgcttg
     1561 tttttcagtc ctggtaagtg aggacagata tagggaagaa gacacgaatg cacgtctaga
     1621 tttagtggag tttgtgatac tgcaggcgtt ggtgcgtaac gccatctatt acgccagatg
     1681 atacctgatc ctgtaacatt cggtaatgtt ttgtaagtgt atctgctgta tggtgttaca
     1741 agtcccacgg gtttcagaag gtgacactca tgaactctgt ggttcagagt agggaatgtc
     1801 ttctcggcag tttaaatcga gggggagact tgttttaagt ctaactgtat ctgtttgggt
     1861 ctaaagaaaa acttgaaata aacatatgct tgtgggacac tcctagaatc cccagtacaa
     1921 agaattagct ctacttttaa cacttagaga aggggcgagg aggagtggag ggaatggtgg
     1981 aagagggaga gggagagaga gagagagaga gagagagatc tttacttaca tgtaattact
     2041 ccaagaagtg gcataggaac agtgggtagt aacaggaaaa atggcagaaa ggcacagttg
     2101 agcctttcta gcagggtagg gtgttgtgtg ttgtgttaca ggagttggcc ggagtacaga
     2161 ctaggtgtgc tctgttctcc agtgtcccac acggtcccca gtccctagca gcctcagaag
     2221 agccccaaag ccctgtgctg gcccagtagt aatgtgaacc catggccttg gaaaggaggc
     2281 tccgtagctc ctaatgagac ttagttaagc ttcctgcagt ttctctggac tttcttttgt
     2341 gccttgtctg ctcgctccct ggtttgtgtc accacagcat ttgtagagcc acaggaggga
     2401 gttgttttta caaggaagcc aggattcgag ctggaaatct gaaacatttc agtggttgtc
     2461 agtaaaatca ctgtcagtaa tgccctggaa aggagcgagt cacataaaat cagcagttct
     2521 tcatggtcct cactgtagca caagtttctt tggttttggt ggggctgagt ttgagacagg
     2581 ttttcccttc tgcccgcctc ctccccactc tcttccctct gctgctgcct ctcagatgct
     2641 gtgattatag gctcgagcac agcacacctg ccatctagtg tgaacttgca catgattaaa
     2701 gtgtgaagaa gccggtaaaa ccaactcgtg ggcatttgag gaggcggttc agagtcagaa
     2761 gtggaagggc acagggaagt gcatttgctt ctgtggtgga agcaagtcac tttccctcag
     2821 tgacaccagc tgccacttag aaggttagcc atctttgacc cttctagcct ctgatttcct
     2881 gtttgatgtg tactttagaa cagggagcgg ggttgtattg gagggctaac atctgagaac
     2941 tgctctctgc tgttctctgg cttgacatag gctggtgttt gcttgattac ttgcctttgt
     3001 gcagctagta tactaagaat tgttttgcat tttagaggtt aaaaaaaaaa ccccaaaaac
     3061 aaaaaaccac cattaccaca aagcataaag tatttactgt ctgacctgtc aggaaaaaga
     3121 aacctctcag agccagtcaa tcgggcagtt tctgatttct gttctcttga attttctggg
     3181 gaaagcttca agtcctgcat tggaagcact ttagtgtaga taggggctgc ctgacagagt
     3241 gatcaggact gttccaccct ctactttgtc cttctagaca gaagcctgtt tcagctcccc
     3301 tcaccctgcc ccacaagtcc tcagccttgc ctctgcacac cacccgttct gtctttgcgt
     3361 aacattgaga actcagtcct ccatgacacc ttgtgtctgg atactcacac ccgagtttat
     3421 catattaaat acaaattttg tataaagagg gagtatcaat gaaaacttga atcaaaaaat
     3481 aagacatgcc agagatgtgg ttttttttta ttgatggact tggtctccta aaaggctgtt
     3541 ttattagttc ctcacatctt gagggatagg tttttttgtt gattgattta gttttggttt
     3601 ttgcagggct tgatattgag accagggctc tagcacagag ctgcatcccc agctccaggg
     3661 cagggcattg gttgcatttg ctataatctt gacaccatcc tagaacagct gttgttattt
     3721 atgcagccca acctctgaga atcatgtgta ttccgtgtct ggtttccttt tccttttttt
     3781 ttttttaagt aaaattaaat ttgactgatg tataaacaat gtcgggttta ggtaggtaag
     3841 gaagggtgtg tttcttgttg gaggttgctg ctgtgatttt acaatttgct gtcttattgt
     3901 ccaatggttt accattttga accacttaca ccatgtcctt acaaatgcct tttcctgtca
     3961 cctgggagag gtgttacgtc aatgcattct agaatgagta agctacactt agcagcttaa
     4021 atgtaattac aatctgaaat ttattttcct ttaccagctt aattctaagg aagcttgaat
     4081 atgggacctg tgcagtacag gtgttaaagt tatgtttcct ttttcctaaa tgcctgagac
     4141 cagcggtgtt tcagatttta gattaaaaaa atgttttgaa atatttgcat atatattatg
     4201 taatctatat tttatacata tatatgtata tacaaacagg gagggggaag atagagatgg
     4261 aacccagcta gagctataaa aatccctttg tgtatcacat gggcctgccc tatgcacaca
     4321 gcttgaagga cggtgtgctc agtgttttag tgtgtctgag agttaactga cctgtgtgca
     4381 caggaggcca gttgtgcagt tctcacctag gtcgtgccac tcacagttgg atttctggag
     4441 cattttggtc agtcaacctg gactgcgaac tagtaagttg ctgtgtattc tgtgactgta
     4501 gctaatattt agcttgcctc aaattttttg agctgatatt tagtcttaac aaattctcct
     4561 tattggatga actgcacagg aagtttttct tttcaaccac gtctctctat gacataaacg
     4621 ttggggtact tagggtaaag tagacactgc atgtgtttat gaaggtgcaa acagaaagaa
     4681 gggtgtcaag tgctacagtt gcattgctgt tgctgtttgt aaactgtgcc gtctcagcgt
     4741 ttgggtgagt aaatgtttgc aaccactgga gaagagtaaa ggccctgtgt gtggtgctac
     4801 atcctcctag gatttagttc acacagacta tgccaggatg gaggggcacc tgaaaactgc
     4861 cctgggagtt ttggaggaag ggcttctaaa atacttaaaa atacgtgaat cacttcagtc
     4921 tgtttaaacg gaaaaaaata tttgatacca ggtctttata tatatatata aatatatata
     4981 aaagaatagt tgtctcaaga ggaataaaat ctggagtgta aaaaaaatgt taaaaaaaaa
     5041 gaatacttgt ctcactttat aatatttttt aaaggaaaaa agaaaacgtt tccaatagct
     5101 tttgtgttct gtgagctctc actttttacc tgaagcagtc ttctgtagca tgtcccacag
     5161 catcagagta acttgatggt gactatcatt tttgtgccca ttatcaggct ccagtcatct
     5221 gtgagagggg ataaactacc ttgctactaa cttaggcctt ttgtgatcta atgacatagc
     5281 tgagggtggt gtgttcgggt atataagagg ttagtatatt caaaaacaat ctctgctatt
     5341 gtggttataa ttatttttgt agtgttttaa tagatgcaca ttaaagtttg taaaaaaaat
     5401 ctaaaatgtc tgtgtgttta tctaatagca gttattttta atgaaatagc taaaaataga
     5461 gatttcataa aataaagcat aaccacccaa a
//
