LOCUS       NM_178079               3540 bp    mRNA    linear   ROD 17-JUL-2024
DEFINITION  Mus musculus peptidase M20 domain containing 1 (Pm20d1), transcript
            variant 1, mRNA.
ACCESSION   NM_178079 XM_897284 XM_923114 XM_976652
VERSION     NM_178079.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Song,N., Fang,Y., Zhu,H., Liu,J., Jiang,S., Sun,S., Xu,R., Ding,J.,
            Hu,G. and Lu,M.
  TITLE     Kir6.2 is essential to maintain neurite features by modulating
            PM20D1-reduced mitochondrial ATP generation
  JOURNAL   Redox Biol 47, 102168 (2021)
   PUBMED   34673451
  REMARK    GeneRIF: Kir6.2 is essential to maintain neurite features by
            modulating PM20D1-reduced mitochondrial ATP generation.
REFERENCE   3  (bases 1 to 3540)
  AUTHORS   Zhang,X., Wang,R., Wang,T., Zhang,X., Dongye,M., Wang,D., Wang,J.,
            Li,W., Wu,X., Lin,D. and Lin,H.
  TITLE     The Metabolic Reprogramming of Frem2 Mutant Mice Embryos in
            Cryptophthalmos Development
  JOURNAL   Front Cell Dev Biol 8, 625492 (2021)
   PUBMED   33490088
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3540)
  AUTHORS   Kim,J.T., Jedrychowski,M.P., Wei,W., Fernandez,D., Fischer,C.R.,
            Banik,S.M., Spiegelman,B.M. and Long,J.Z.
  TITLE     A Plasma Protein Network Regulates PM20D1 and N-Acyl Amino Acid
            Bioactivity
  JOURNAL   Cell Chem Biol 27 (9), 1130-1139 (2020)
   PUBMED   32402239
REFERENCE   5  (bases 1 to 3540)
  AUTHORS   Li,D., Liu,Y., Gao,W., Han,J., Yuan,R., Zhang,M. and Pang,W.
  TITLE     Inhibition of miR-324-5p increases PM20D1-mediated white and brown
            adipose loss and reduces body weight in juvenile mice
  JOURNAL   Eur J Pharmacol 863, 172708 (2019)
   PUBMED   31568785
  REMARK    GeneRIF: Inhibition of miR-324-5p increases PM20D1-mediated white
            and brown adipose loss and reduces body weight in juvenile mice
REFERENCE   6  (bases 1 to 3540)
  AUTHORS   Long,J.Z., Roche,A.M., Berdan,C.A., Louie,S.M., Roberts,A.J.,
            Svensson,K.J., Dou,F.Y., Bateman,L.A., Mina,A.I., Deng,Z.,
            Jedrychowski,M.P., Lin,H., Kamenecka,T.M., Asara,J.M.,
            Griffin,P.R., Banks,A.S., Nomura,D.K. and Spiegelman,B.M.
  TITLE     Ablation of PM20D1 reveals N-acyl amino acid control of metabolism
            and nociception
  JOURNAL   Proc Natl Acad Sci U S A 115 (29), E6937-E6945 (2018)
   PUBMED   29967167
  REMARK    GeneRIF: These data demonstrate that PM20D1 is a dominant enzymatic
            regulator of NAA levels in vivo and elucidate physiologic functions
            for NAA signaling in metabolism and nociception.
REFERENCE   7  (bases 1 to 3540)
  AUTHORS   Long,J.Z., Svensson,K.J., Bateman,L.A., Lin,H., Kamenecka,T.,
            Lokurkar,I.A., Lou,J., Rao,R.R., Chang,M.R., Jedrychowski,M.P.,
            Paulo,J.A., Gygi,S.P., Griffin,P.R., Nomura,D.K. and
            Spiegelman,B.M.
  TITLE     The Secreted Enzyme PM20D1 Regulates Lipidated Amino Acid
            Uncouplers of Mitochondria
  JOURNAL   Cell 166 (2), 424-435 (2016)
   PUBMED   27374330
  REMARK    GeneRIF: Study demonstrates that PM20D1 is a bidirectional enzyme
            in vitro, catalyzing both the condensation of fatty acids and amino
            acids to generate N-acyl amino acids and also the reverse
            hydrolytic reaction. N-acyl amino acids directly bind mitochondria
            and function as endogenous uncouplers of UCP1-independent
            respiration.
REFERENCE   8  (bases 1 to 3540)
  AUTHORS   Tamplin,O.J., Cox,B.J. and Rossant,J.
  TITLE     Integrated microarray and ChIP analysis identifies multiple Foxa2
            dependent target genes in the notochord
  JOURNAL   Dev Biol 360 (2), 415-425 (2011)
   PUBMED   22008794
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC161805.4.
            
            On Apr 28, 2009 this sequence version replaced NM_178079.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC120725.1, AK143953.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-192               AC161805.4         135094-135285       c
            193-279             AC161805.4         133998-134084       c
            280-512             AC161805.4         131354-131586       c
            513-599             AC161805.4         130868-130954       c
            600-733             AC161805.4         130246-130379       c
            734-853             AC161805.4         129841-129960       c
            854-929             AC161805.4         128553-128628       c
            930-991             AC161805.4         128032-128093       c
            992-1070            AC161805.4         127709-127787       c
            1071-1142           AC161805.4         125710-125781       c
            1143-1311           AC161805.4         120477-120645       c
            1312-1411           AC161805.4         118690-118789       c
            1412-3540           AC161805.4         114565-116693       c
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 57.19 cM"
     gene            1..3540
                     /gene="Pm20d1"
                     /gene_synonym="4732466D17Rik"
                     /note="peptidase M20 domain containing 1"
                     /db_xref="GeneID:212933"
                     /db_xref="MGI:MGI:2442939"
     exon            1..192
                     /gene="Pm20d1"
                     /gene_synonym="4732466D17Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             24..1535
                     /gene="Pm20d1"
                     /gene_synonym="4732466D17Rik"
                     /EC_number="3.5.1.14"
                     /EC_number="3.5.1.114"
                     /note="probable carboxypeptidase PM20D1; peptidase M20
                     domain-containing protein 1; N-fatty-acyl-amino acid
                     synthase/hydrolase PM20D1"
                     /codon_start=1
                     /product="N-fatty-acyl-amino acid synthase/hydrolase
                     PM20D1 precursor"
                     /protein_id="NP_835180.2"
                     /db_xref="CCDS:CCDS15274.1"
                     /db_xref="GeneID:212933"
                     /db_xref="MGI:MGI:2442939"
                     /translation="MAELLASLPAWAAVLLLFFATVSGSTGPRSRENRGASRIPSQFS
                     EEERVAIKEALKGAIQIPTVSFSHEESNTTALAEFGEYIRKAFPTVFHSSLVQHEVVA
                     KYSHLFTIQGSDPSLQPYMLMAHIDVVPAPEEGWEVPPFSGLERNGFIYGRGALDNKN
                     SVMAILHALELLLIRNYSPKRSFFIALGHDEEVSGEKGAQKISALLQARGVQLAFLVD
                     EGSFILEGFIPNLEKPVAMISVTEKGALDLMLQVNMTPGHSSAPPKETSIGILSAAVS
                     RLEQTPMPNMFGGGPLKKTMKLLANEFSFPINIVLRNLWLFHPIVSRIMERNPITNAL
                     VRTTTALTMFNAGIKVNVIPPLAQATINCRIHPSQTVHEVLELVKNTVADDRVQLHVL
                     RSFEPLPISPSDDQAMGYQLLQETIRSVFPEVDIVVPGICIANTDTRHYANITNGMYR
                     FNPLPLNPQDFSGVHGINEKVSVQNYQNQVKFIFEFIQNADTYKEPVPHLHEL"
     exon            193..279
                     /gene="Pm20d1"
                     /gene_synonym="4732466D17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            280..512
                     /gene="Pm20d1"
                     /gene_synonym="4732466D17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            513..599
                     /gene="Pm20d1"
                     /gene_synonym="4732466D17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            600..733
                     /gene="Pm20d1"
                     /gene_synonym="4732466D17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            734..853
                     /gene="Pm20d1"
                     /gene_synonym="4732466D17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            854..929
                     /gene="Pm20d1"
                     /gene_synonym="4732466D17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            930..991
                     /gene="Pm20d1"
                     /gene_synonym="4732466D17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            992..1070
                     /gene="Pm20d1"
                     /gene_synonym="4732466D17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1071..1142
                     /gene="Pm20d1"
                     /gene_synonym="4732466D17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1143..1311
                     /gene="Pm20d1"
                     /gene_synonym="4732466D17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1312..1411
                     /gene="Pm20d1"
                     /gene_synonym="4732466D17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1412..3540
                     /gene="Pm20d1"
                     /gene_synonym="4732466D17Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3519..3524
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pm20d1"
                     /gene_synonym="4732466D17Rik"
                     /note="hexamer: AATAAA"
     polyA_site      3539
                     /gene="Pm20d1"
                     /gene_synonym="4732466D17Rik"
                     /note="major polyA site"
ORIGIN      
        1 actggtggct tttggggaga gacatggctg agctacttgc tagcttgccc gcctgggcag
       61 ctgtgctcct tctctttttc gctacggtct ccggatccac tggccctaga agcagggaaa
      121 atcggggggc gtcccggatc ccttcccagt tcagcgagga ggagcgtgtc gctataaaag
      181 aggcgctgaa aggtgccatc cagattccca cagtgtcttt cagccacgag gaatccaaca
      241 ccacagccct tgctgagttt ggagaatata tccgcaaagc cttccctaca gtgttccaca
      301 gcagccttgt ccaacatgaa gtcgtggcaa agtatagcca cctgttcacc atccaaggct
      361 cagaccccag tttgcagccc tacatgctga tggctcacat tgatgtggtt cctgccccgg
      421 aagaaggatg ggaggtgccc ccgttctcag gcctggaacg caatggcttc atctatggcc
      481 ggggtgcgct ggacaacaaa aactctgtga tggcgatcct gcatgctttg gagctcctgt
      541 tgatcagaaa ctacagcccc aaaagatctt tcttcattgc tttgggccat gatgaggagg
      601 tgtccgggga aaagggggct cagaagatct cagcactctt acaggcaagg ggtgtccagc
      661 tagccttcct tgtggatgaa gggagcttta tcttggaagg cttcattcca aacctcgaga
      721 agccagttgc catgatttca gtcactgaga agggtgccct tgacctcatg ctgcaagtaa
      781 acatgactcc aggccactct tcagctcccc caaaggagac aagcattggc attctttctg
      841 ccgctgtcag ccgactggag cagacaccaa tgccgaatat gtttggagga gggccattga
      901 agaagacaat gaagctactg gcaaatgagt tttccttccc tatcaatata gtcttgagaa
      961 acctgtggct atttcatccc attgtgagca ggataatgga gaggaacccc ataacaaatg
     1021 cgctggtccg aactaccaca gccctcacca tgttcaatgc aggaatcaag gtgaatgtca
     1081 tccctccatt ggctcaggct acaatcaact gccgaattca cccttcgcag acagtacatg
     1141 aggtcctaga acttgtcaag aacaccgtgg ctgatgacag agtccagctg catgtgttga
     1201 gatcctttga acccctgccc atcagcccct ctgatgacca ggccatgggc taccagctgc
     1261 ttcaagagac catacgatct gtcttcccgg aagtcgacat cgtcgtcccc ggtatttgta
     1321 ttgccaatac ggacacccga cactatgcca acatcaccaa tggcatgtac cggttcaacc
     1381 cccttcccct gaaccctcag gacttcagtg gtgtccatgg aatcaatgag aaagtttccg
     1441 ttcagaacta ccagaaccag gtgaagttca tctttgagtt catccaaaat gccgacactt
     1501 acaaagagcc agttcctcat ctgcatgaac tatgaggcaa ggatccagct gggtgaggga
     1561 tgcccagcac tgggctcagg actaacctaa agggagagag agctggtatt aatgaagggt
     1621 ttggtggaaa ccatcctgaa cagagttcac ctgcctgact tccctcctcc actcaccagg
     1681 gattgaggtc ccaggaggaa agggagatga acagaatctg ccctgctgtc ctcctgacat
     1741 ctgcgttctt cctctgagtg gcaactactg ccttctggtg tgtcctgcct gttggtctgt
     1801 ctcatgccga ttattaacta ttccactttc ccagcctgtc ttaacaaata cagattttgg
     1861 aaagacctca agcaggttta aatactagag tattgacttc aggtacctga ggctggactg
     1921 tcttctagaa tgccctttct cctgtcttct ctccgactcg ggagctgccc cttggcctct
     1981 ctcttcctgc ctctgctttc ttcctccaat ctccagctca ttccacatct ggagttaaga
     2041 ctcaaactta gaagctccca tgatcacagt caaatagaag ggcctgtgta atgaaacttg
     2101 aggtggagac tttgagagaa ccccagaact cctggttcca gagacttggg ctcttgggat
     2161 tttgttcccc tgccctgccc tgttcctgcc acctgctgtc cggccccacc ccaggaagca
     2221 ttactctaca gaagcatttc cctggcacag tgtacgggct gagctcccaa gagaggaaca
     2281 ctcaaaatcc agcttctcag cggtgcccag agccagcagc gccctggtcc tcgtgtgtcc
     2341 catttcagaa tgaccaaggg gaagtggagg acaaggagga caagagaagc aaagggaggt
     2401 agcgtccatt aaagtatagc agagctcctg tcttcgctct ggacgtcaac cctaggctat
     2461 ttgaatgtgc ccagtagctg ccaggtgggg ggcactggga agcagccttt cttggctcaa
     2521 gaccagcatc tctgctctgt ggctcacaag ggagtggagg tcacaaaggg agacacagaa
     2581 atggagcttg gaaggctccg tctcttcccc tctccagact aggaccttgt gtgtctccca
     2641 aggctttgac agttctaggt ttgcttgcct cctcctcaga gatctccatt ctccctcact
     2701 cccaagtcct taccttcccc ccaggatatg aacggttcct tgaagctctg gcttaatggg
     2761 taacgtttag aaactgtggg gattattcca gctttttagc tactcctgct aagattcaca
     2821 ttccttcccc gactctccct ttttggacaa ttcagtactt attcagcctt cattgaaggg
     2881 caagcttaag aacaaaaata gtccttcact tttgtgcaga gccctacgag atagtcttat
     2941 agacgcttca tctcagccct gtgaacacgc tatcattact ccttcttaca gagatggact
     3001 cgattctgga aacagtgttc aaaccttcat tactttgttc ccagcctcat cagtattctc
     3061 tgatcctgca tcacctactc cccatcctac atcacctact ccccactgat ttcttcttta
     3121 attctcatga ctactgaggc atgagtgtgt aatcacatga gggaagtatt cacagggagg
     3181 gaacatagcc ggctggggct tgcttttgta gggttattag gattagacaa ggaatggggc
     3241 tgagctccat taattaatgg ctttttaagg agagaatttg agaaaataga cacatttaat
     3301 ctgatttgga aaatgtacca tgaatacatg tgtcaaaata tcacagtact tcactaaatt
     3361 aaggggaaaa gagaacagaa gacagacggg ttcatacata tattccctgt gtcttgccat
     3421 gtgaagtcct ataccatctc ttatgcgagg aaacctattc atcagggggc aaactagtgt
     3481 tccccacatg attttaatgc tccagagctg tgagccaaaa taaaccatgc tcaacccggt
//
