LOCUS       NM_023564               2785 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus phospholipid scramblase 3 (Plscr3), transcript variant
            1, mRNA.
ACCESSION   NM_023564 XM_918341
VERSION     NM_023564.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Inokawa,A., Inuzuka,T., Takahara,T., Shibata,H. and Maki,M.
  TITLE     Tubby-like protein superfamily member PLSCR3 functions as a
            negative regulator of adipogenesis in mouse 3T3-L1 preadipocytes by
            suppressing induction of late differentiation stage transcription
            factors
  JOURNAL   Biosci Rep 36 (1), e00287 (2015)
   PUBMED   26677203
  REMARK    GeneRIF: results suggest that Scr3 functions as a negative
            regulator of adipogenesis in 3T3-L1 cells at a specific
            differentiation stage and that decrease in the intracellular amount
            of Scr3 protein caused by reduction in Scr3 mRNA expression
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   3  (bases 1 to 2785)
  AUTHORS   Merrick,B.A., Dhungana,S., Williams,J.G., Aloor,J.J., Peddada,S.,
            Tomer,K.B. and Fessler,M.B.
  TITLE     Proteomic profiling of S-acylated macrophage proteins identifies a
            role for palmitoylation in mitochondrial targeting of phospholipid
            scramblase 3
  JOURNAL   Mol Cell Proteomics 10 (10), M110.006007 (2011)
   PUBMED   21785166
REFERENCE   4  (bases 1 to 2785)
  AUTHORS   Kowalczyk,J.E., Beresewicz,M., Gajkowska,B. and Zablocka,B.
  TITLE     Association of protein kinase C delta and phospholipid scramblase 3
            in hippocampal mitochondria correlates with neuronal vulnerability
            to brain ischemia
  JOURNAL   Neurochem Int 55 (1-3), 157-163 (2009)
   PUBMED   19428821
REFERENCE   5  (bases 1 to 2785)
  AUTHORS   Lu,B., Sims,P.J., Wiedmer,T., Moser,A.H., Shigenaga,J.K.,
            Grunfeld,C. and Feingold,K.R.
  TITLE     Expression of the phospholipid scramblase (PLSCR) gene family
            during the acute phase response
  JOURNAL   Biochim Biophys Acta 1771 (9), 1177-1185 (2007)
   PUBMED   17590392
REFERENCE   6  (bases 1 to 2785)
  AUTHORS   Mutch,D.M., O'Maille,G., Wikoff,W.R., Wiedmer,T., Sims,P.J. and
            Siuzdak,G.
  TITLE     Mobilization of pro-inflammatory lipids in obese Plscr3-deficient
            mice
  JOURNAL   Genome Biol 8 (3), R38 (2007)
   PUBMED   17355638
  REMARK    GeneRIF: identified previously unrecognized lipid metabolites that
            suggest a novel molecular link between obesity, inflammation and
            the downstream consequences associated with PLSCR3-deficiency
REFERENCE   7  (bases 1 to 2785)
  AUTHORS   Wiedmer,T., Zhao,J., Li,L., Zhou,Q., Hevener,A., Olefsky,J.M.,
            Curtiss,L.K. and Sims,P.J.
  TITLE     Adiposity, dyslipidemia, and insulin resistance in mice with
            targeted deletion of phospholipid scramblase 3 (PLSCR3)
  JOURNAL   Proc Natl Acad Sci U S A 101 (36), 13296-13301 (2004)
   PUBMED   15328404
  REMARK    GeneRIF: expression of PLSCR3 may be required for normal adipocyte
            and/or macrophage maturation or function
REFERENCE   8  (bases 1 to 2785)
  AUTHORS   Wiedmer,T., Zhou,Q., Kwoh,D.Y. and Sims,P.J.
  TITLE     Identification of three new members of the phospholipid scramblase
            gene family
  JOURNAL   Biochim Biophys Acta 1467 (1), 244-253 (2000)
   PUBMED   10930526
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL603707.5 and AK011716.1.
            
            On Dec 15, 2009 this sequence version replaced NM_023564.3.
            
            Transcript Variant: This variant (1) encodes the functional
            protein. Both variants 1 and 2 encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK172635.1, AK166288.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-33                AL603707.5         225846-225878
            34-112              AK011716.1         33-111
            113-113             AL603707.5         226174-226174
            114-373             AK011716.1         112-371
            374-374             AL603707.5         226694-226694
            375-1352            AK011716.1         373-1350
            1353-2785           AL603707.5         229855-231287
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 42.9 cM"
     gene            1..2785
                     /gene="Plscr3"
                     /gene_synonym="2210403O21Rik; 2610037N06Rik; ESTM3"
                     /note="phospholipid scramblase 3"
                     /db_xref="GeneID:70310"
                     /db_xref="MGI:MGI:1917560"
     exon            1..75
                     /gene="Plscr3"
                     /gene_synonym="2210403O21Rik; 2610037N06Rik; ESTM3"
                     /inference="alignment:Splign:2.1.0"
     exon            76..243
                     /gene="Plscr3"
                     /gene_synonym="2210403O21Rik; 2610037N06Rik; ESTM3"
                     /inference="alignment:Splign:2.1.0"
     CDS             237..1127
                     /gene="Plscr3"
                     /gene_synonym="2210403O21Rik; 2610037N06Rik; ESTM3"
                     /note="Ca 2+ dependent phospholipid scramblase 3; PL
                     scramblase 3; ca(2+)-dependent phospholipid scramblase 3"
                     /codon_start=1
                     /product="phospholipid scramblase 3"
                     /protein_id="NP_076053.1"
                     /db_xref="CCDS:CCDS24916.1"
                     /db_xref="GeneID:70310"
                     /db_xref="MGI:MGI:1917560"
                     /translation="MAGYLPPKGYAPSPPPPYPVPSGYPEPVALHPGPGQAPVPTQVP
                     APAPGFALFPSPGPVAPGPPAPFVPLPGVPPGLEFLVQIDQILIHQKAERVETFLGWE
                     TCNMYELRSGTGQQLGQAAEESNCCARLCCGARRPFRIRLADPGDREVLRLLRPLHCG
                     CSCCPCGLQEMEVQAPPGTTIGHVLQTWHPFLPKFSILDADRQPVLRVVGPCWTCGCG
                     TDTNFEVKTKDESRSVGRISKQWGGLLREALTDADDFGLQFPVDLDVKVKAVLLGATF
                     LIDYMFFEKRGGAGPSAITS"
     exon            244..482
                     /gene="Plscr3"
                     /gene_synonym="2210403O21Rik; 2610037N06Rik; ESTM3"
                     /inference="alignment:Splign:2.1.0"
     exon            483..525
                     /gene="Plscr3"
                     /gene_synonym="2210403O21Rik; 2610037N06Rik; ESTM3"
                     /inference="alignment:Splign:2.1.0"
     exon            526..746
                     /gene="Plscr3"
                     /gene_synonym="2210403O21Rik; 2610037N06Rik; ESTM3"
                     /inference="alignment:Splign:2.1.0"
     exon            747..908
                     /gene="Plscr3"
                     /gene_synonym="2210403O21Rik; 2610037N06Rik; ESTM3"
                     /inference="alignment:Splign:2.1.0"
     exon            909..1070
                     /gene="Plscr3"
                     /gene_synonym="2210403O21Rik; 2610037N06Rik; ESTM3"
                     /inference="alignment:Splign:2.1.0"
     exon            1071..2785
                     /gene="Plscr3"
                     /gene_synonym="2210403O21Rik; 2610037N06Rik; ESTM3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1744..1749
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Plscr3"
                     /gene_synonym="2210403O21Rik; 2610037N06Rik; ESTM3"
                     /note="hexamer: TATAAA"
     polyA_site      1771
                     /gene="Plscr3"
                     /gene_synonym="2210403O21Rik; 2610037N06Rik; ESTM3"
                     /note="major polyA site"
     regulatory      1857..1862
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Plscr3"
                     /gene_synonym="2210403O21Rik; 2610037N06Rik; ESTM3"
                     /note="hexamer: ATTAAA"
     polyA_site      1887
                     /gene="Plscr3"
                     /gene_synonym="2210403O21Rik; 2610037N06Rik; ESTM3"
     regulatory      2675..2680
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Plscr3"
                     /gene_synonym="2210403O21Rik; 2610037N06Rik; ESTM3"
                     /note="hexamer: CATAAA"
     polyA_site      2693
                     /gene="Plscr3"
                     /gene_synonym="2210403O21Rik; 2610037N06Rik; ESTM3"
ORIGIN      
        1 agatctgggc ccgcctccac cagcgcctgt gagccccgga gtgcgtggcc ccccgactta
       61 ttctgggggc ttcaggtgcc gccctagaga ccctgggccc gtactgggcg cagctacctc
      121 ttcgcctctg cctgtccgtc tttgtttctg tgtctgtcta gctgttcccg agcttgtccc
      181 actccagaac taagtctccc ctacgccaaa agcccaagac tcccctcctg attcccatgg
      241 caggctactt gccccccaaa ggctatgccc cttcaccccc acctccctac cccgtgccat
      301 ctgggtatcc agagccggtg gctctgcatc ctggaccggg acaagctcca gtgcccaccc
      361 aggtgcctgc ccctgctccc ggcttcgctc tcttcccctc gccaggccca gtggctccag
      421 ggcctcctgc tcctttcgtg ccattgccag gggtgcctcc tggcctcgaa ttcctagtgc
      481 agattgatca aatcttgatt catcagaagg ctgaacgagt ggaaacgttc ctaggctggg
      541 agacctgtaa tatgtatgaa cttcgctccg gaaccggaca gcaactgggt caggcagctg
      601 aagagagcaa ctgttgtgcc cgcctgtgct gtggtgcccg ccgaccattt cgaatccgcc
      661 tagcggaccc tggggaccgc gaggtgctcc ggctcctccg cccacttcat tgtggctgca
      721 gctgctgccc ctgtggtctt caggagatgg aagtccaggc tccacctggc accaccattg
      781 gccatgtgct acagacctgg catcccttcc ttcctaagtt ttccatcctg gatgctgatc
      841 gccaacctgt tctacgagtt gtagggcctt gctggacttg tggctgtggt acagacacca
      901 actttgaggt gaagactaag gatgaatcgc gaagtgtggg ccgcatcagc aagcagtggg
      961 gagggctgct ccgagaagcc ctcacagatg ccgatgactt tggcctccaa ttcccagtcg
     1021 atctagatgt gaaagtgaag gccgtactgc tgggagccac gttcctcatc gattatatgt
     1081 tcttcgagaa gagaggaggc gcaggaccct ctgccatcac cagttagaag ccacctcagg
     1141 atgaggagac ccatctcctt gaccagaatt taagatggtc agctgccctg gacgttccct
     1201 cctgaagcaa ccctttcctt gatatacact gcggcggacc gacgagggtg gccgagtggt
     1261 tgggagccgt tgtgtcccat cccttccctg cttctcctgt ggccctgcag aagagcatgt
     1321 atgagacctg ttcctccttc tgttcaccat ctccaggcag tgcctgtgca cacattagct
     1381 tttaaacttc cttgcacact ccttccagcc ttcctctggg gcctctgcat aggcaggggc
     1441 atctggaatc ctggactcaa gttttacccc agggcttgtg ggtaaaaggc aagcagtacc
     1501 aaagatggca gacaccaccc ttcccttatg gcactttagc caattagttt agcttccgat
     1561 tgtggcactc tgaggggatc cttgcctcct cactaatagc tgtagcggtt gggccccagt
     1621 gccaactccc taagcccctg ggccctgcgg gtgctttctg cagcttcctg tgccttattt
     1681 aaccgttaac cccttccttc ccctactgta ggaaggaggc tgtgtctttg tatgttgtac
     1741 tcatataaac tttgaaactt tttaaacagt agaagccgga tgtgatggga gagggtagat
     1801 accggataac acaaagggag cggcgtcagc cagcctgtat aatctacact atttggatta
     1861 aaggaaaaaa gtccttgagt ccaaaaagtt agttttcatc acccatagtg attctggacc
     1921 aaaccagagc agctcccaag tcaatatcta agttgggtgc cccatagagt ttagggaagt
     1981 agctaatgac agttccgacg gttggtcagt gatccggtat tgctacttga tccttgtctg
     2041 tagtgagtag aggcctcctc caaagctttt tctttcaagt cctgttcaat tgggaatata
     2101 gtaggcagag tggacttggt cttggtccct tcagaggcca aggagcaggg ctcggaggaa
     2161 gcattagcca gtacctgagt gccctataca cttcctgtca ctcctcacag caggtaaatc
     2221 tccctcagcg tgcctccgtc aagacctcat gttctgatta gtccgtaagg ccaagcccta
     2281 agtggaccag gtaacccatg ctggtgtcca tccctgagga tacagagttc aggaccgggc
     2341 taggatgtag cgactggctg ctgacaggtc ccactggtga tgctccagga tgcgacgaca
     2401 gtctgctcgg gaccggttac taaggtggaa gagctggtct acctgtgtga gcaggaggta
     2461 ggacagctga ccactagtgg gccaaacagg cgactgggga acctgcagaa ggggttagcg
     2521 ttaccttgag gttccggata gcagaagcca catctccccc agtggttctg agtgccactt
     2581 ggcactcctg gtaggtgaca ccatgcacac tcagctctac ctgcggagga caaatctttg
     2641 tttagccctt gtgtcaacct acaggccctt tgaacataaa gtcatttcag tgagccctca
     2701 cctctacaac cttcctctgc cactcggggt ctgacaaggg acctccagag gggacagtgg
     2761 ctttgctggc tccaggtgct ccagt
//
