LOCUS       NM_008880               1895 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus phospholipid scramblase 2 (Plscr2), transcript variant
            B, mRNA.
ACCESSION   NM_008880
VERSION     NM_008880.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1895)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   4  (bases 1 to 1895)
  AUTHORS   Lu,B., Sims,P.J., Wiedmer,T., Moser,A.H., Shigenaga,J.K.,
            Grunfeld,C. and Feingold,K.R.
  TITLE     Expression of the phospholipid scramblase (PLSCR) gene family
            during the acute phase response
  JOURNAL   Biochim Biophys Acta 1771 (9), 1177-1185 (2007)
   PUBMED   17590392
REFERENCE   5  (bases 1 to 1895)
  AUTHORS   Bailey,P.J., Klos,J.M., Andersson,E., Karlen,M., Kallstrom,M.,
            Ponjavic,J., Muhr,J., Lenhard,B., Sandelin,A. and Ericson,J.
  TITLE     A global genomic transcriptional code associated with CNS-expressed
            genes
  JOURNAL   Exp Cell Res 312 (16), 3108-3119 (2006)
   PUBMED   16919269
REFERENCE   6  (bases 1 to 1895)
  AUTHORS   Yu,A., McMaster,C.R., Byers,D.M., Ridgway,N.D. and Cook,H.W.
  TITLE     Stimulation of phosphatidylserine biosynthesis and facilitation of
            UV-induced apoptosis in Chinese hamster ovary cells overexpressing
            phospholipid scramblase 1
  JOURNAL   J Biol Chem 278 (11), 9706-9714 (2003)
   PUBMED   12509439
  REMARK    GeneRIF: PLSCR2 is expressed predominantly in the cell nucleus
REFERENCE   7  (bases 1 to 1895)
  AUTHORS   Suzuki,H., Fukunishi,Y., Kagawa,I., Saito,R., Oda,H., Endo,T.,
            Kondo,S., Bono,H., Okazaki,Y. and Hayashizaki,Y.
  TITLE     Protein-protein interaction panel using mouse full-length cDNAs
  JOURNAL   Genome Res 11 (10), 1758-1765 (2001)
   PUBMED   11591653
REFERENCE   8  (bases 1 to 1895)
  AUTHORS   Wiedmer,T., Zhou,Q., Kwoh,D.Y. and Sims,P.J.
  TITLE     Identification of three new members of the phospholipid scramblase
            gene family
  JOURNAL   Biochim Biophys Acta 1467 (1), 244-253 (2000)
   PUBMED   10930526
REFERENCE   9  (bases 1 to 1895)
  AUTHORS   Zhou,Q., Sims,P.J. and Wiedmer,T.
  TITLE     Identity of a conserved motif in phospholipid scramblase that is
            required for Ca2+-accelerated transbilayer movement of membrane
            phospholipids
  JOURNAL   Biochemistry 37 (8), 2356-2360 (1998)
   PUBMED   9485382
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC111125.11, AK002410.1 and AW261539.1.
            
            On Aug 21, 2010 this sequence version replaced NM_008880.2.
            
            Transcript Variant: This variant (B) differs in the 5' UTR compared
            to variant A. Both variants A and B encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC011299.1, AK002410.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-114               AC111125.11        54284-54397         c
            115-972             AK002410.1         2-859
            973-973             AC111125.11        39157-39157         c
            974-1042            AK002410.1         861-929
            1043-1061           AC111125.11        38952-38970         c
            1062-1868           AK002410.1         949-1755
            1869-1895           AW261539.1         1-27                c
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 48.35 cM"
     gene            1..1895
                     /gene="Plscr2"
                     /note="phospholipid scramblase 2"
                     /db_xref="GeneID:18828"
                     /db_xref="MGI:MGI:1270860"
     exon            1..412
                     /gene="Plscr2"
                     /inference="alignment:Splign:2.1.0"
     exon            413..493
                     /gene="Plscr2"
                     /inference="alignment:Splign:2.1.0"
     CDS             421..1344
                     /gene="Plscr2"
                     /note="PL scramblase 2; ca(2+)-dependent phospholipid
                     scramblase 2"
                     /codon_start=1
                     /product="phospholipid scramblase 2"
                     /protein_id="NP_032906.2"
                     /db_xref="CCDS:CCDS23405.1"
                     /db_xref="GeneID:18828"
                     /db_xref="MGI:MGI:1270860"
                     /translation="MEAPRSGTYLPAGYAPQYPPAAVQGPPEHTGRPTFQTNYQVPQS
                     GYPGPQASYTVSTSGHEGYAATRLPIQNNQTIVLANTQWMPAPPPILNCPPGLEYLNQ
                     IDQLLIHQQVELLEVLTGFETNNKFEIKNSLGQMVYVAVEDTDCCTRNCCEASRPFTL
                     RILDHLGQEVMTLERPLKCSSCCFPCCLQEIEIQAPPGVPIGYVTQTWHPCLPKLTLQ
                     NDKRENVLKVVGPCVACTCCSDIDFEIKSLDEVTRIGKITKQWSGCVKEAFTDSDNFG
                     IQFPLDLEVKMKAVTLGACFLIDYMFFEGCE"
     exon            494..726
                     /gene="Plscr2"
                     /inference="alignment:Splign:2.1.0"
     exon            727..769
                     /gene="Plscr2"
                     /inference="alignment:Splign:2.1.0"
     exon            770..990
                     /gene="Plscr2"
                     /inference="alignment:Splign:2.1.0"
     exon            991..1152
                     /gene="Plscr2"
                     /inference="alignment:Splign:2.1.0"
     exon            1153..1314
                     /gene="Plscr2"
                     /inference="alignment:Splign:2.1.0"
     exon            1315..1895
                     /gene="Plscr2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1854..1859
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Plscr2"
                     /note="hexamer: AATAAA"
     polyA_site      1874
                     /gene="Plscr2"
                     /note="major polyA site"
ORIGIN      
        1 ggaaagcatt cctgagtctc cttttgaatc agtgccagca tttgaagaat gcagtggagg
       61 tcagagtaaa gggaatgcca gtaagtgttc ctttggcgtt ccagaactgt gcacagacca
      121 gatattcatt tctactgaga gcctctgcca ggagcagcta acaattctca aaggacttag
      181 aactcagaca gggtttcaat ttggttgcgt atctttccag tcaggaaaag gaaatctaaa
      241 gactcaggaa acaaaaccta aactgcctca aagtccaggt gctttttctc cctgacttta
      301 gtctagtgga gtagtgcagc acctatgcct ttctgagagg agtctggaga gctgagtcgc
      361 tgctggtgct aggattctag gaattcgcct cacttggagc tgcatgagaa aggcttgcaa
      421 atggaggctc ctcgctcagg aacatacttg ccagctgggt atgcccctca gtatcctcca
      481 gcagcagtcc aaggacctcc agagcatact ggacgcccca cattccagac taactaccaa
      541 gttccccagt ctggttatcc aggacctcag gctagctaca cagtctcaac atctggacat
      601 gaaggttatg ctgctacacg gcttcctatt caaaataatc agactatagt ccttgcaaac
      661 actcagtgga tgccagcacc accacctatt ctgaactgcc cacctgggct agaatactta
      721 aatcagatag atcagcttct gattcatcag caagttgaac ttctagaagt cttaacaggc
      781 tttgaaacaa ataacaaatt tgaaatcaag aacagcctcg ggcagatggt ttatgttgca
      841 gtggaagata ctgactgctg tactcgaaat tgctgtgaag cgtctagacc tttcacttta
      901 agaatcctgg atcatctggg ccaagaagtc atgactctgg agagacctct gaaatgcagt
      961 agctgctgct tcccttgctg cctccaggag atagaaatcc aggctcctcc aggggtgcca
     1021 ataggttatg tgactcagac ctggcaccca tgtctgccaa agctcactct tcagaacgac
     1081 aagagggaga atgttctaaa agtagttggt ccatgtgttg catgcacctg ctgttcagat
     1141 attgactttg agatcaagtc tcttgatgaa gtgactagaa ttggtaagat caccaagcag
     1201 tggtctggtt gtgtgaaaga ggccttcacg gattcggata actttgggat ccaattcccg
     1261 ctagacctgg aggtgaagat gaaagctgtg acgcttggtg cttgcttcct catagattac
     1321 atgttttttg aaggctgtga gtaggaacag aaatccgacc tgcagtagga atcaatgaaa
     1381 gaggacagag aagatctgaa gtctacacaa ggagatcata tgattgagag acctggggct
     1441 ttttgatttc ttcattgaaa tttctcagaa tcaagctgtt atacatgaag catagtatgt
     1501 aacattttgg ttttcaaatg gtagtttatc ttttacatta ttggaataga cctggataat
     1561 tatctttata cacttctaaa aatatgcacc aaattcaagt taaaaaaaaa agacgaagag
     1621 aagtgtatgt tttaaaataa aacattttat ggaaaagtaa gttaaatcat aatctttgat
     1681 ttatttttca tcttttgttc aatttaaacc ttgttagtgc tgattttatt ataaaattgt
     1741 actttactat caaacctagt tagtttattt cttacagaaa tcctcctatt attttgaaat
     1801 tacatatttt tgaaagcttt ttaaaagata ctattgcctg ggaaattcta ttcaataaaa
     1861 tgctaatgag aaaagatagt gtgtactttc ttcca
//
