LOCUS       NM_001410621            3305 bp    mRNA    linear   ROD 13-JUN-2024
DEFINITION  Mus musculus polo like kinase 4 (Plk4), transcript variant 4, mRNA.
ACCESSION   NM_001410621 XM_006500768
VERSION     NM_001410621.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Man,C.H., Lam,W., Dang,C.C., Zeng,X.Y., Zheng,L.C., Chan,N.N.,
            Ng,K.L., Chan,K.C., Kwok,T.H., Ng,T.C., Leung,W.Y., Huen,M.S.,
            Wong,C.C., So,C.W.E., Dou,Z., Goyama,S., Bray,M.R., Mak,T.W. and
            Leung,A.Y.
  TITLE     Inhibition of PLK4 remodels histone methylation and activates the
            immune response via the cGAS-STING pathway in TP53-mutated AML
  JOURNAL   Blood 142 (23), 2002-2015 (2023)
   PUBMED   37738460
  REMARK    GeneRIF: Inhibition of PLK4 remodels histone methylation and
            activates the immune response via the cGAS-STING pathway in
            TP53-mutated AML.
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Sladky,V.C., Akbari,H., Tapias-Gomez,D., Evans,L.T., Drown,C.G.,
            Strong,M.A., LoMastro,G.M., Larman,T. and Holland,A.J.
  TITLE     Centriole signaling restricts hepatocyte ploidy to maintain liver
            integrity
  JOURNAL   Genes Dev 36 (13-14), 843-856 (2022)
   PUBMED   35981754
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   3  (bases 1 to 3305)
  AUTHORS   LoMastro,G.M., Drown,C.G., Maryniak,A.L., Jewett,C.E., Strong,M.A.
            and Holland,A.J.
  TITLE     PLK4 drives centriole amplification and apical surface area
            expansion in multiciliated cells
  JOURNAL   Elife 11, e80643 (2022)
   PUBMED   35969030
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3305)
  AUTHORS   Phan,T.P., Boatwright,C.A., Drown,C.G., Skinner,M.W., Strong,M.A.,
            Jordan,P.W. and Holland,A.J.
  TITLE     Upstream open reading frames control PLK4 translation and centriole
            duplication in primordial germ cells
  JOURNAL   Genes Dev 36 (11-12), 718-736 (2022)
   PUBMED   35772791
  REMARK    GeneRIF: Upstream open reading frames control PLK4 translation and
            centriole duplication in primordial germ cells.
REFERENCE   5  (bases 1 to 3305)
  AUTHORS   Hamada,N., Iwamoto,I., Noda,M., Nishikawa,M. and Nagata,K.I.
  TITLE     Expression Analyses of Polo-Like Kinase 4, a Gene Product
            Responsible for Autosomal Recessive Microcephaly and Seckel
            Syndrome, during Mouse Brain Development
  JOURNAL   Dev Neurosci 44 (6), 643-650 (2022)
   PUBMED   36067731
  REMARK    GeneRIF: Expression Analyses of Polo-Like Kinase 4, a Gene Product
            Responsible for Autosomal Recessive Microcephaly and Seckel
            Syndrome, during Mouse Brain Development.
REFERENCE   6  (bases 1 to 3305)
  AUTHORS   Leung,G.C., Hudson,J.W., Kozarova,A., Davidson,A., Dennis,J.W. and
            Sicheri,F.
  TITLE     The Sak polo-box comprises a structural domain sufficient for
            mitotic subcellular localization
  JOURNAL   Nat Struct Biol 9 (10), 719-724 (2002)
   PUBMED   12352953
  REMARK    GeneRIF: 2.0 A crystal structure of a novel domain composed of the
            polo box motif of murine Sak
REFERENCE   7  (bases 1 to 3305)
  AUTHORS   Hudson,J.W., Kozarova,A., Cheung,P., Macmillan,J.C., Swallow,C.J.,
            Cross,J.C. and Dennis,J.W.
  TITLE     Late mitotic failure in mice lacking Sak, a polo-like kinase
  JOURNAL   Curr Biol 11 (6), 441-446 (2001)
   PUBMED   11301255
REFERENCE   8  (bases 1 to 3305)
  AUTHORS   Hudson,J.W., Chen,L., Fode,C., Binkert,C. and Dennis,J.W.
  TITLE     Sak kinase gene structure and transcriptional regulation
  JOURNAL   Gene 241 (1), 65-73 (2000)
   PUBMED   10607900
REFERENCE   9  (bases 1 to 3305)
  AUTHORS   Fode,C., Binkert,C. and Dennis,J.W.
  TITLE     Constitutive expression of murine Sak-a suppresses cell growth and
            induces multinucleation
  JOURNAL   Mol Cell Biol 16 (9), 4665-4672 (1996)
   PUBMED   8756623
REFERENCE   10 (bases 1 to 3305)
  AUTHORS   Fode,C., Motro,B., Yousefi,S., Heffernan,M. and Dennis,J.W.
  TITLE     Sak, a murine protein-serine/threonine kinase that is related to
            the Drosophila polo kinase and involved in cell proliferation
  JOURNAL   Proc Natl Acad Sci U S A 91 (14), 6388-6392 (1994)
   PUBMED   8022793
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC160757.3.
            
            On Aug 13, 2022 this sequence version replaced XM_006500768.4.
            
            Summary: This gene encodes a member of the polo family of
            serine/threonine protein kinases. The protein localizes to the
            nucleolus during G2, to centrosomes during G2/M, and to the
            cleavage furrow during cytokinesis. It is required for progression
            through mitosis, cell survival, and embryonic development. The
            mouse genome contains a pseudogene similar to this gene. Three
            transcript variants encoding two different isoforms have been found
            for this gene. [provided by RefSeq, Nov 2011].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR17253014.578559.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-91                AC160757.3         143773-143863       c
            92-187              AC160757.3         143549-143644       c
            188-283             AC160757.3         142764-142859       c
            284-398             AC160757.3         142572-142686       c
            399-1311            AC160757.3         138724-139636       c
            1312-1412           AC160757.3         138057-138157       c
            1413-1774           AC160757.3         135536-135897       c
            1775-1879           AC160757.3         134263-134367       c
            1880-1982           AC160757.3         133880-133982       c
            1983-2132           AC160757.3         133261-133410       c
            2133-2266           AC160757.3         132631-132764       c
            2267-2358           AC160757.3         132459-132550       c
            2359-2503           AC160757.3         131769-131913       c
            2504-2629           AC160757.3         131053-131178       c
            2630-2736           AC160757.3         129556-129662       c
            2737-3305           AC160757.3         127761-128329       c
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 19.54 cM"
     gene            1..3305
                     /gene="Plk4"
                     /gene_synonym="1700028H20; Sak; Stk18"
                     /note="polo like kinase 4"
                     /db_xref="GeneID:20873"
                     /db_xref="MGI:MGI:101783"
     exon            1..91
                     /gene="Plk4"
                     /gene_synonym="1700028H20; Sak; Stk18"
                     /inference="alignment:Splign:2.1.0"
     CDS             59..2839
                     /gene="Plk4"
                     /gene_synonym="1700028H20; Sak; Stk18"
                     /EC_number="2.7.11.21"
                     /note="isoform 3 is encoded by transcript variant 4;
                     serine/threonine-protein kinase Plk4;
                     serine/threonine-protein kinase 18;
                     serine/threonine-protein kinase Sak; serine/threonine
                     kinase 18"
                     /codon_start=1
                     /product="serine/threonine-protein kinase PLK4 isoform 3"
                     /protein_id="NP_001397550.1"
                     /db_xref="GeneID:20873"
                     /db_xref="MGI:MGI:101783"
                     /translation="MPSTPDTCIIEDFKVGNLLGKGSFAGVYRAESIHTGLEVAIKMI
                     DKKAMYKAGMVQRVQNEVKIHCQLKHPSVLELYNYFEDNNYVYLVLEMCHNGEMNRYL
                     KNRMKPFSEREARHFMHQIITGMLYLHSHGILHRDLTLSNILLTRNMNIKIADFGLAT
                     QLNMPHEKHYTLCGTPNYISPEIATRSAHGLESDIWSLGCMFYTLLIGRPPFDTDTVK
                     NTLNKVVLADYEMPAFLSREAQDLIHQLLRRNPADRLSLSSVLDHPFMSRNPSPKSKD
                     VGTVEDSMDSGHATLSTTITASSGTSLSGSLLDRRLLVGQPLPNKITVFQKNKNSSDF
                     SSGDGSNFCTQWGNPEQEANSRGRGRVIEDAEERPHSRYLRRAHSSDRASPSNQSRAK
                     TYSVERCHSVEMLSKPRRSLDENQHSSNHHCLGKTPFPFADQTPQMEMVQQWFGNLQM
                     NAHLGETNEHHTVSPNRDFQDYPDLQDTLRNAWTDTRASKNADTSANVHAVKQLSAMK
                     YMSAHHHKPEVMPQEPGLHPHSEQSKNRSMESTLGYQKPTLRSITSPLIAHRLKPIRQ
                     KTKKAVVSILDSEEVCVELLRECASEGYVKEVLQISSDGTMITVYYPNDGRGFPLADR
                     PPLPTDNISRYSFDNLPEKYWRKYQYASRFIQLVRSKTPKITYFTRYAKCILMENSPG
                     ADFEVWFYDGAKIHKTENLIHIIEKTGISYNLKNENEVTSLKEEVKVYMDHANEGHRI
                     CLSLESVISEEEKRSRGSSFFPIIVGRKPGNTSSPKALSAPPVDPSCCKGEQASASRL
                     SVNSAAFPTQSPGLSPSTVTVEGLGHTATATGTGVSSSLPKSAQLLKSVFVKNVGWAT
                     QLTSGAVWVQFNDGSQLVVQAGVSSISYTSPDGQTTRYGENEKLPEYIKQKLQCLSSI
                     LLMFSNPTPNFQ"
     exon            92..187
                     /gene="Plk4"
                     /gene_synonym="1700028H20; Sak; Stk18"
                     /inference="alignment:Splign:2.1.0"
     exon            188..283
                     /gene="Plk4"
                     /gene_synonym="1700028H20; Sak; Stk18"
                     /inference="alignment:Splign:2.1.0"
     exon            284..398
                     /gene="Plk4"
                     /gene_synonym="1700028H20; Sak; Stk18"
                     /inference="alignment:Splign:2.1.0"
     exon            399..1311
                     /gene="Plk4"
                     /gene_synonym="1700028H20; Sak; Stk18"
                     /inference="alignment:Splign:2.1.0"
     exon            1312..1412
                     /gene="Plk4"
                     /gene_synonym="1700028H20; Sak; Stk18"
                     /inference="alignment:Splign:2.1.0"
     exon            1413..1774
                     /gene="Plk4"
                     /gene_synonym="1700028H20; Sak; Stk18"
                     /inference="alignment:Splign:2.1.0"
     exon            1775..1879
                     /gene="Plk4"
                     /gene_synonym="1700028H20; Sak; Stk18"
                     /inference="alignment:Splign:2.1.0"
     exon            1880..1982
                     /gene="Plk4"
                     /gene_synonym="1700028H20; Sak; Stk18"
                     /inference="alignment:Splign:2.1.0"
     exon            1983..2132
                     /gene="Plk4"
                     /gene_synonym="1700028H20; Sak; Stk18"
                     /inference="alignment:Splign:2.1.0"
     exon            2133..2266
                     /gene="Plk4"
                     /gene_synonym="1700028H20; Sak; Stk18"
                     /inference="alignment:Splign:2.1.0"
     exon            2267..2358
                     /gene="Plk4"
                     /gene_synonym="1700028H20; Sak; Stk18"
                     /inference="alignment:Splign:2.1.0"
     exon            2359..2503
                     /gene="Plk4"
                     /gene_synonym="1700028H20; Sak; Stk18"
                     /inference="alignment:Splign:2.1.0"
     exon            2504..2629
                     /gene="Plk4"
                     /gene_synonym="1700028H20; Sak; Stk18"
                     /inference="alignment:Splign:2.1.0"
     exon            2630..2736
                     /gene="Plk4"
                     /gene_synonym="1700028H20; Sak; Stk18"
                     /inference="alignment:Splign:2.1.0"
     exon            2737..3305
                     /gene="Plk4"
                     /gene_synonym="1700028H20; Sak; Stk18"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3264..3269
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Plk4"
                     /gene_synonym="1700028H20; Sak; Stk18"
                     /note="hexamer: AATACA"
     regulatory      3278..3283
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Plk4"
                     /gene_synonym="1700028H20; Sak; Stk18"
                     /note="hexamer: AATAAA"
     polyA_site      3283
                     /gene="Plk4"
                     /gene_synonym="1700028H20; Sak; Stk18"
     polyA_site      3305
                     /gene="Plk4"
                     /gene_synonym="1700028H20; Sak; Stk18"
                     /note="major polyA site"
ORIGIN      
        1 attagagtcc ttagcctcca tcagggaggg agctcagaaa atctctcaga gcgtcaagat
       61 gccttccacc ccagacacct gcatcatcga ggactttaag gttggaaatc tactcggtaa
      121 aggatcattt gctggtgtct acagagctga gtccatacac actggtttgg aagttgcaat
      181 caaaatgata gataagaaag ccatgtacaa agctggaatg gtacagagag tccaaaatga
      241 ggtgaaaata cattgccagt tgaaacaccc ctctgtcttg gagctctata attactttga
      301 agataacaat tatgtctacc tggtattgga aatgtgccac aatggagaaa tgaacagata
      361 tctgaagaac agaatgaagc ctttctcaga aagggaagct aggcacttca tgcaccagat
      421 tatcacagga atgttatatc ttcattctca tggcatattg caccgggacc tcacactctc
      481 taacatctta cttacgcgga atatgaacat aaaaattgct gactttggac tagcaacgca
      541 gttgaatatg ccacatgaaa agcactatac actctgtggg actcctaatt atatttcacc
      601 agaaattgca actcgaagtg cacatggact tgaatctgat atttggtcat tgggctgtat
      661 gttttatacg ttacttattg gaagaccacc ttttgacact gacacagtca agaacacatt
      721 gaacaaagta gtcctggcag attatgaaat gccagccttt ttgtcacgag aggcccagga
      781 ccttatccac cagttacttc gtagaaaccc tgcagatcgg ttaagtctgt cttctgtgtt
      841 ggaccatcct ttcatgtcac gaaatccttc accaaagagt aaagacgtag ggactgtaga
      901 ggactcaatg gatagtgggc atgctacact ttccacaaca attacagcct cttctggtac
      961 cagtttgagt ggcagcctac ttgacagaag acttttggtt ggtcaaccac ttccaaataa
     1021 aattactgta tttcaaaaaa ataaaaattc aagtgacttt tcttcaggag atggaagtaa
     1081 tttttgtact caatggggaa atccagaaca agaagctaat agtaggggac gggggagagt
     1141 gattgaagat gcagaagaga ggccgcattc tcgatacctg cgcagagctc attcctctga
     1201 tagagccagc ccctctaatc agtctcgagc aaaaacatac tcagtagaac gttgtcactc
     1261 agtagaaatg ctttcaaagc ctagaagatc actggatgaa aatcaacaca gttccaatca
     1321 tcattgtcta ggaaaaactc cttttccatt tgcagaccag acacctcaga tggaaatggt
     1381 acagcagtgg tttgggaatc tgcaaatgaa tgctcattta ggagaaacta atgagcacca
     1441 caccgttagc ccaaacagag atttccagga ctatccagat ttgcaggaca cgttacgaaa
     1501 cgcttggact gacacgagag ccagcaagaa tgctgatact tctgccaatg ttcatgctgt
     1561 aaagcagctg agtgccatga aatacatgag tgcacatcac cataagcctg aggtcatgcc
     1621 acaggagccg ggcctacatc ctcattctga acaaagcaag aatagaagta tggagtcgac
     1681 actgggttac cagaaaccta ccttaagaag tattacatct cctctgattg ctcacagatt
     1741 aaagccaatc agacagaaaa ccaaaaaggc tgtggtgagc atccttgatt cagaggaggt
     1801 gtgtgtggag cttctgagag agtgtgcgtc tgaaggatat gtgaaagaag tgcttcagat
     1861 atcgagtgat gggactatga tcactgttta ttacccgaac gatggaagag gctttcctct
     1921 tgctgacaga cctcccttgc ctactgacaa catcagtagg tacagctttg acaatctacc
     1981 agaaaaatac tggcggaaat atcagtatgc ttccagattc attcagctag taagatctaa
     2041 aactcccaaa atcacttatt ttacaagata tgctaaatgt attttgatgg aaaattctcc
     2101 tggtgctgat ttcgaagttt ggttttatga tggagccaaa atacataaaa ctgaaaattt
     2161 aattcacata attgagaaaa cagggatatc ttataattta aaaaatgaaa atgaagttac
     2221 cagcctgaaa gaggaagtaa aagtatatat ggaccatgct aatgagggtc accgtatttg
     2281 cttgtcactg gaatctgtaa tctctgagga ggaaaagaga agcaggggtt cttcattctt
     2341 ccctataatc gtaggaagaa aacctggtaa tactagttca cctaaagcct tatcagctcc
     2401 tcctgtggac ccaagctgct gtaagggaga gcaggcgtca gcaagcagac tgagcgtgaa
     2461 tagtgccgct ttccccacac agtccccagg actcagtcct tccactgtga cagttgaagg
     2521 acttggccac acagcgactg ccacaggaac aggcgtctct tcaagtcttc ctaaatctgc
     2581 acagcttttg aaatctgttt ttgtgaaaaa tgttggttgg gctacacagc taactagcgg
     2641 agctgtgtgg gttcagttta atgatgggtc acagttggtt gtccaggcag gagtatcttc
     2701 catcagttac acatcaccag atggtcagac aactaggtat ggagaaaatg aaaaattacc
     2761 tgaatacatc aaacagaaat tacagtgtct ttcttccatc cttctgatgt tttctaatcc
     2821 aactcctaat tttcagtaat ttaagtctca gaagtctata tttaataaat gactttttgg
     2881 ctggctttca agtaagtgat tttttaaatt tactttaact tcagaaagcc tttctattaa
     2941 acagaatttt aatatacaca ataaaaatat aataagaaaa caataaaatt tcagttacct
     3001 aatatagtgg tcataaggct aggacatcta attttgctcc aagcatgtaa tccttcaaag
     3061 tttgtgctcc tatgtttgta ttgaactaag ttgtgtatgg cttgtttgtt tttgttattt
     3121 tctttactaa taagacattg agaatcacgg acaaaacata gttttcaatt tttgaatgtg
     3181 taaataatgt attataagca atatgtaaat gtgtatattt tatatttatt tttatagcac
     3241 ttgtgtctga taagatttct gcaaatacat tttataaaat aaacacagtg gtaagttttc
     3301 cttaa
//
