LOCUS       NM_001033150            4163 bp    mRNA    linear   ROD 09-JUL-2024
DEFINITION  Mus musculus pleckstrin homology domain containing, family M (with
            RUN domain) member 2 (Plekhm2), transcript variant 1, mRNA.
ACCESSION   NM_001033150 XM_924800 XM_980218
VERSION     NM_001033150.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Etzion,S., Hijaze,R., Segal,L., Pilcha,S., Masil,D., Levi,O.,
            Elyagon,S., Levitas,A., Etzion,Y. and Parvari,R.
  TITLE     Plekhm2 acts as an autophagy modulator in murine heart and
            cardiofibroblasts
  JOURNAL   Sci Rep 14 (1), 14949 (2024)
   PUBMED   38942823
  REMARK    GeneRIF: Plekhm2 acts as an autophagy modulator in murine heart and
            cardiofibroblasts.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4163)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 4163)
  AUTHORS   Zou,W., Lv,Y., Zhang,S., Li,L., Sun,L. and Jiao,J.
  TITLE     Lysosomal dynamics regulate mammalian cortical neurogenesis
  JOURNAL   Dev Cell 59 (1), 64-78 (2024)
   PUBMED   38103552
REFERENCE   4  (bases 1 to 4163)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4163)
  AUTHORS   Saitoh,S.I., Abe,F., Kanno,A., Tanimura,N., Mori Saitoh,Y.,
            Fukui,R., Shibata,T., Sato,K., Ichinohe,T., Hayashi,M., Kubota,K.,
            Kozuka-Hata,H., Oyama,M., Kikko,Y., Katada,T., Kontani,K. and
            Miyake,K.
  TITLE     TLR7 mediated viral recognition results in focal type I interferon
            secretion by dendritic cells
  JOURNAL   Nat Commun 8 (1), 1592 (2017)
   PUBMED   29150602
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4163)
  AUTHORS   Zhao,W., Moest,T., Zhao,Y., Guilhon,A.A., Buffat,C., Gorvel,J.P.
            and Meresse,S.
  TITLE     The Salmonella effector protein SifA plays a dual role in virulence
  JOURNAL   Sci Rep 5, 12979 (2015)
   PUBMED   26268777
  REMARK    GeneRIF: We used a model of SKIP knockout mice to show that this
            protein mediates the host susceptibility to salmonellosis and to
            establish that SifA also contributes to Salmonella virulence
            independently of its interaction with SKIP
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 4163)
  AUTHORS   Diacovich,L., Dumont,A., Lafitte,D., Soprano,E., Guilhon,A.A.,
            Bignon,C., Gorvel,J.P., Bourne,Y. and Meresse,S.
  TITLE     Interaction between the SifA virulence factor and its host target
            SKIP is essential for Salmonella pathogenesis
  JOURNAL   J Biol Chem 284 (48), 33151-33160 (2009)
   PUBMED   19801640
  REMARK    GeneRIF: Data shoe that the N-terminal domain of SifA is sufficient
            to interact with the pleckstrin homology domain of SKIP, forming a
            1:1 complex with a micromolar dissociation constant.
REFERENCE   8  (bases 1 to 4163)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Aizawa,H., Yuasa,S.,
            Nakajima,D., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: II. The complete nucleotide sequences of 400 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (1), 35-48 (2003)
   PUBMED   12693553
REFERENCE   9  (bases 1 to 4163)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL670446.8.
            
            On Mar 21, 2019 this sequence version replaced NM_001033150.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660811.165727.1,
                                           SRR14995047.765600.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-286               AL670446.8         168150-168435       c
            287-393             AL670446.8         146939-147045       c
            394-503             AL670446.8         146695-146804       c
            504-603             AL670446.8         146284-146383       c
            604-691             AL670446.8         145706-145793       c
            692-878             AL670446.8         143965-144151       c
            879-938             AL670446.8         141714-141773       c
            939-1164            AL670446.8         138542-138767       c
            1165-2028           AL670446.8         136002-136865       c
            2029-2098           AL670446.8         135799-135868       c
            2099-2189           AL670446.8         135579-135669       c
            2190-2254           AL670446.8         135281-135345       c
            2255-2343           AL670446.8         134861-134949       c
            2344-2476           AL670446.8         134199-134331       c
            2477-2667           AL670446.8         133739-133929       c
            2668-2733           AL670446.8         133370-133435       c
            2734-2893           AL670446.8         132570-132729       c
            2894-3073           AL670446.8         132238-132417       c
            3074-3190           AL670446.8         132040-132156       c
            3191-4163           AL670446.8         130024-130996       c
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 74.64 cM"
     gene            1..4163
                     /gene="Plekhm2"
                     /gene_synonym="2310034J19Rik; mKIAA0842"
                     /note="pleckstrin homology domain containing, family M
                     (with RUN domain) member 2"
                     /db_xref="GeneID:69582"
                     /db_xref="MGI:MGI:1916832"
     exon            1..286
                     /gene="Plekhm2"
                     /gene_synonym="2310034J19Rik; mKIAA0842"
                     /inference="alignment:Splign:2.1.0"
     CDS             227..3328
                     /gene="Plekhm2"
                     /gene_synonym="2310034J19Rik; mKIAA0842"
                     /note="isoform 1 is encoded by transcript variant 1;
                     pleckstrin homology domain-containing family M member 2;
                     PH domain-containing family M member 2"
                     /codon_start=1
                     /product="pleckstrin homology domain-containing family M
                     member 2 isoform 1"
                     /protein_id="NP_001028322.1"
                     /db_xref="CCDS:CCDS51348.1"
                     /db_xref="GeneID:69582"
                     /db_xref="MGI:MGI:1916832"
                     /translation="MEPREVKDRILENISLSVKKLQSYFAACEDETPAIRNHDKVLQR
                     LCEHLDHALLYGLQDLSSGYWVLVVHFTRREAIRQIEVLQHVATNLGRSRAWLYLALN
                     ENSLESYLRLFQENLGLLQKYYVRNALVCSHDHLTLFLTLVSGLEFIRFDLDLDAPYL
                     DLAPYMPDYYKPQYLLDFEDRLPSSVHGSDSLSLNSFNSVTSTNLEWDDSAIAPSSED
                     YDFGDVFPAVPSVPSTDWEDGDLTDTISGPRSTASDLTSSKTSTKSPTQRHNPFNEEQ
                     AETASSDTTPVHTTSQEKEEAQAPDQPDACTELEVIRVTKKKKIGKKKKTKLDEDASP
                     LHPTSSQQKCGQQGEGDGLVGTPGLARDPSDTVLASPQEQGEGLSSTAGSSELSELSQ
                     MGLLIPEMKDTSMECLGQPLSKVIDKLHGQLDPSTWCSHADPPEQSFRAGSPGEAPEK
                     PPFCDFSEGLPAPMDFYRFTVESPSTVAPGGGHHDPPGPSQPLHVPGSPAAALQEEEE
                     GGGRGEGQTSQPLEDRQGEEIQEPEPQEPDSQLPLVSQEPVPEPVSQPEPGTHEALCK
                     LKRDQPSPCLSSAEDSGVEEGQGSPSEMTHPSEFRVDNNHLLLLMIHVFRENEEQLFK
                     MIRMSTGHMEGNLQLLYVLLTDCYVYLLRKGATEKPYLVEEAVSYNELDYVSVGLDQQ
                     TVKLVCTNRRKQFLLDTADVALAELFLASLKSAMIKGCREPPYPSILTDATMEKLALA
                     KFVAQESKCEASAVTVHFYGLVHWEDPMEEALGPVPCQCSPAEGTITKEGMLHYKAST
                     SYLGKEHWKACFVVLSNGILYQYPDRTDVIPLLSVNMGGEQCGGCRRSNTTDRPHAFQ
                     VILADRPCLELSADSEAEMADWMQHLCQAVSKGVIPQGIAPSPCIPCCLVITEDRLFT
                     CHEDCQTSFFRSLGTARLADITAISTELGKEYCVLEFSQDSPQLLQPWVIYLSCTSEL
                     DRFLTALSSGWKAIYQVDLPHKAIHEASIKQKFEDALSLIHSAWQRSDSLCRGRASRD
                     PWC"
     exon            287..393
                     /gene="Plekhm2"
                     /gene_synonym="2310034J19Rik; mKIAA0842"
                     /inference="alignment:Splign:2.1.0"
     exon            394..503
                     /gene="Plekhm2"
                     /gene_synonym="2310034J19Rik; mKIAA0842"
                     /inference="alignment:Splign:2.1.0"
     exon            504..603
                     /gene="Plekhm2"
                     /gene_synonym="2310034J19Rik; mKIAA0842"
                     /inference="alignment:Splign:2.1.0"
     exon            604..691
                     /gene="Plekhm2"
                     /gene_synonym="2310034J19Rik; mKIAA0842"
                     /inference="alignment:Splign:2.1.0"
     exon            692..878
                     /gene="Plekhm2"
                     /gene_synonym="2310034J19Rik; mKIAA0842"
                     /inference="alignment:Splign:2.1.0"
     exon            879..938
                     /gene="Plekhm2"
                     /gene_synonym="2310034J19Rik; mKIAA0842"
                     /inference="alignment:Splign:2.1.0"
     exon            939..1164
                     /gene="Plekhm2"
                     /gene_synonym="2310034J19Rik; mKIAA0842"
                     /inference="alignment:Splign:2.1.0"
     exon            1165..2028
                     /gene="Plekhm2"
                     /gene_synonym="2310034J19Rik; mKIAA0842"
                     /inference="alignment:Splign:2.1.0"
     exon            2029..2098
                     /gene="Plekhm2"
                     /gene_synonym="2310034J19Rik; mKIAA0842"
                     /inference="alignment:Splign:2.1.0"
     exon            2099..2189
                     /gene="Plekhm2"
                     /gene_synonym="2310034J19Rik; mKIAA0842"
                     /inference="alignment:Splign:2.1.0"
     exon            2190..2254
                     /gene="Plekhm2"
                     /gene_synonym="2310034J19Rik; mKIAA0842"
                     /inference="alignment:Splign:2.1.0"
     exon            2255..2343
                     /gene="Plekhm2"
                     /gene_synonym="2310034J19Rik; mKIAA0842"
                     /inference="alignment:Splign:2.1.0"
     exon            2344..2476
                     /gene="Plekhm2"
                     /gene_synonym="2310034J19Rik; mKIAA0842"
                     /inference="alignment:Splign:2.1.0"
     exon            2477..2667
                     /gene="Plekhm2"
                     /gene_synonym="2310034J19Rik; mKIAA0842"
                     /inference="alignment:Splign:2.1.0"
     exon            2668..2733
                     /gene="Plekhm2"
                     /gene_synonym="2310034J19Rik; mKIAA0842"
                     /inference="alignment:Splign:2.1.0"
     exon            2734..2893
                     /gene="Plekhm2"
                     /gene_synonym="2310034J19Rik; mKIAA0842"
                     /inference="alignment:Splign:2.1.0"
     exon            2894..3073
                     /gene="Plekhm2"
                     /gene_synonym="2310034J19Rik; mKIAA0842"
                     /inference="alignment:Splign:2.1.0"
     exon            3074..3190
                     /gene="Plekhm2"
                     /gene_synonym="2310034J19Rik; mKIAA0842"
                     /inference="alignment:Splign:2.1.0"
     exon            3191..4163
                     /gene="Plekhm2"
                     /gene_synonym="2310034J19Rik; mKIAA0842"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agttcctgcg gccgcgcagg gcccgcgtct ccttcccggc tcggcgctcg ggaggcggcg
       61 gcggcggagc gaggcccggg cgaggcgagg cgaggcgaga cgagggcggc gggcgccggg
      121 gcccgcggcc ggcacgacgg agcccccgca cggccggcgg cagcggttgg cggcggcccc
      181 gggctcccgg cgtgggaagc ggcggcgggg cggcggcggc agcgccatgg agccgcggga
      241 ggtgaaggac cgaatcctgg agaacatctc gctgtcggtg aagaagctgc agagctactt
      301 tgcagcatgt gaggacgaga cccctgccat ccggaaccac gacaaggtcc tgcagcgcct
      361 gtgtgagcac ctggaccacg ccctgctgta cgggttgcaa gacttgtcct ctggctactg
      421 ggtgctcgtg gtccatttta cccgcagaga ggccatcagg caaattgagg tgctacagca
      481 tgtagccacc aacctggggc gaagccgagc ctggctctac ctggccctca atgagaattc
      541 cttggagagc tacctgcgtc tgttccagga gaacctgggc ctgctacaga agtactatgt
      601 caggaacgcc ctggtctgca gccacgatca cctgactctc ttcctgacct tggtgtctgg
      661 gttggagttt attcggttcg acctggatct ggacgccccg tacttagacc tggctccgta
      721 catgcctgac tattataaac ctcagtacct gctggacttt gaagaccgcc ttcctagctc
      781 agtccacggt tcagacagcc tctccctaaa ctccttcaat tctgtcacct ccaccaacct
      841 ggaatgggac gacagtgcaa ttgccccatc tagcgaggat tatgattttg gagatgtgtt
      901 tccagcagtg ccgtctgtac ccagcacaga ctgggaagat ggagacctca cggacaccat
      961 cagtggtccg cgttccactg cttcagacct gaccagtagc aagacatcca ccaagagccc
     1021 cacccagcgt cataacccct tcaatgagga gcaagcagag actgcatcat ccgacaccac
     1081 ccctgtgcac actacctctc aggagaagga ggaggcccag gccccagacc agccagacgc
     1141 ttgtacagag cttgaagtta tcagggtcac caagaagaag aagattggca agaagaaaaa
     1201 gaccaagctg gacgaggatg caagcccact gcaccccacc tctagtcagc agaaatgtgg
     1261 ccagcagggg gaaggtgatg gacttgtggg caccccaggc ctggcgaggg acccttcgga
     1321 cactgtactt gcttcccctc aggagcaagg ggaaggactc agcagtacgg ctgggagcag
     1381 cgagctctcc gagctcagcc agatgggctt gctcatcccc gagatgaagg acacctccat
     1441 ggagtgccta ggacagcctc tgagcaaggt cattgacaaa ctccacggcc agctggaccc
     1501 tagcacctgg tgctcccatg ccgatcctcc cgagcagtcc tttcgggccg gctctccggg
     1561 ggaggccccg gagaaaccac cattttgcga ctttagtgag gggcttccag cccccatgga
     1621 cttctaccgg tttacagtcg agagtccaag tactgttgca ccaggtggcg gccaccatga
     1681 ccctccaggg cctagccaac cgctgcatgt tcctggtagc cctgcggctg ctctccaaga
     1741 agaagaagaa ggaggaggaa gaggagaggg acagacatct cagcccctag aagaccggca
     1801 aggagaggag attcaggagc cagagcctca ggaaccggac agccagttgc cactggtcag
     1861 ccaggagccc gtgcccgaac ctgtgtccca gcctgaacct ggaacccacg aggctctttg
     1921 caagctcaag cgagaccagc ccagtccttg tctgagcagt gctgaggact ccggggtaga
     1981 ggagggccag ggtagccctt cagagatgac gcacccctca gagttcagag tagacaacaa
     2041 ccacttgctc ctcctcatga ttcacgtctt tcgagaaaac gaagagcagt tgttcaaaat
     2101 gatccggatg agcacagggc acatggaggg taatctgcaa ctgctctacg tgctactcac
     2161 agactgctat gtctacctgc ttcggaaagg ggccacagaa aagccatacc tggtggaaga
     2221 ggctgtttct tacaatgaac ttgactatgt gtcggttggc ctcgaccaac agactgtcaa
     2281 gctggtgtgc accaaccgca ggaagcagtt tctgctggac acagccgacg tggccctggc
     2341 tgagctcttc ttggcgtctc tgaagtcagc catgatcaaa ggttgccggg aaccacccta
     2401 ccccagcatc ctgactgatg ccaccatgga gaagctggcg ttggcgaaat ttgtcgccca
     2461 ggagtccaaa tgtgaggcat ccgccgtgac cgtgcatttc tatgggcttg tgcactggga
     2521 ggaccccatg gaagaggccc tgggccctgt tccctgccag tgctcacccg ctgagggcac
     2581 catcaccaaa gaaggcatgc tgcactacaa ggctagcacc tcctacctgg gcaaggaaca
     2641 ctggaaggcc tgcttcgtgg tgctcagcaa tgggatcctc taccagtatc ctgaccgcac
     2701 ggacgtcatc cccctgctgt ccgtgaacat gggtggggaa cagtgcggtg gctgccggag
     2761 atccaacacc acggatcggc cccacgcctt ccaggtcatt ctggctgacc ggccctgcct
     2821 ggagctaagc gccgacagcg aggctgagat ggctgactgg atgcaacatc tctgccaggc
     2881 cgtgtccaaa ggagtcatac cccagggcat agctcccagc ccctgtatcc cttgctgcct
     2941 ggtgatcaca gaggaccgcc tcttcacctg ccacgaggat tgccaaacca gcttctttcg
     3001 ctccctgggc acagccaggc tggcagacat cactgccatc tccacggagc tgggcaagga
     3061 atactgtgtc ctggagttct cccaggatag cccacagctg ctccagcctt gggtcatcta
     3121 cctgagctgc acttccgagc tggaccggtt cctgactgcc ctgagctctg ggtggaaagc
     3181 catctaccag gtagaccttc ctcacaaggc catccatgaa gcttccatca agcagaaatt
     3241 tgaagacgcc ctgagcctca tccacagcgc ctggcagcgg agcgacagtc tctgccgcgg
     3301 cagggcctcc cgggacccct ggtgctgagg cgtggctggg cagcactgtg ggcagaccag
     3361 aaggaggcag gctgactgac ttctgcactg cgttgtccca ctgtggtgtt tcggggccgg
     3421 gggtcgacag cccactgctg ccttggggcg gcagaaccac atagtgcggc tagagacagg
     3481 agttcagagt cagtgcctgg caccgtaggc accctgccca ctggtagtga ctagcggtca
     3541 ctggggcaga aggtctgagc cctgtgggct ctgtagacac ttggtgtccc ctggccacca
     3601 ttgagtcctc accatctgct cagggtgaca gagagaatga caacatacat ttaagaggga
     3661 gcaaagagac ggggagggtg ggtgcgccat cttcctttcc tgggtcaggg gcctgggcgg
     3721 atggtcaggg ccccctgggg ggtcctgagt gcccagccac ccttgttcat ttttgaacag
     3781 tgtcccaatc acacaaagaa agacaaacac tagggatctg tgcatgcccc cctcacaaat
     3841 ccaccccatg cacagcctgg gcccagcccg cagccctcca gggctgccag gaggccctgc
     3901 atgtccacag ccccgcctct cctcttcctc aaatacagca gagcttgtag aaccaggggt
     3961 cagttggctc ctcttgccag gagcaaagga gagggtcaga gagaggagcg gagcccatga
     4021 gcgtggtggc ttctttgaca tctgacgacc aacccctgct tggctggggc tggcccttct
     4081 tgaggtctct gttaggtccc ttgagggaca ggtcaggtgg attgggtttt gttttttaaa
     4141 ataaaattga tgttatattg ctg
//
