LOCUS       NM_001374783            3842 bp    mRNA    linear   ROD 12-OCT-2024
DEFINITION  Mus musculus pleckstrin homology domain containing, family G (with
            RhoGef domain) member 5 (Plekhg5), transcript variant 3, mRNA.
ACCESSION   NM_001374783 XM_006538926
VERSION     NM_001374783.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Hutchings,A.J., Hambrecht,B., Veh,A., Giridhar,N.J., Zare,A.,
            Angerer,C., Ohnesorge,T., Schenke,M., Selvaraj,B.T., Chandran,S.,
            Sterneckert,J., Petri,S., Seeger,B., Briese,M., Stigloher,C.,
            Bischler,T., Hermann,A., Damme,M., Sendtner,M. and Luningschror,P.
  TITLE     Plekhg5 controls the unconventional secretion of Sod1 by
            presynaptic secretory autophagy
  JOURNAL   Nat Commun 15 (1), 8622 (2024)
   PUBMED   39366938
  REMARK    GeneRIF: Plekhg5 controls the unconventional secretion of Sod1 by
            presynaptic secretory autophagy.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Luningschror,P., Slotta,C., Heimann,P., Briese,M., Weikert,U.M.,
            Massih,B., Appenzeller,S., Sendtner,M., Kaltschmidt,C. and
            Kaltschmidt,B.
  TITLE     Absence of Plekhg5 Results in Myelin Infoldings Corresponding to an
            Impaired Schwann Cell Autophagy, and a Reduced T-Cell Infiltration
            Into Peripheral Nerves
  JOURNAL   Front Cell Neurosci 14, 185 (2020)
   PUBMED   32733205
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3842)
  AUTHORS   Luningschror,P., Binotti,B., Dombert,B., Heimann,P., Perez-Lara,A.,
            Slotta,C., Thau-Habermann,N., R von Collenberg,C., Karl,F.,
            Damme,M., Horowitz,A., Maystadt,I., Fuchtbauer,A., Fuchtbauer,E.M.,
            Jablonka,S., Blum,R., Uceyler,N., Petri,S., Kaltschmidt,B.,
            Jahn,R., Kaltschmidt,C. and Sendtner,M.
  TITLE     Plekhg5-regulated autophagy of synaptic vesicles reveals a
            pathogenic mechanism in motoneuron disease
  JOURNAL   Nat Commun 8 (1), 678 (2017)
   PUBMED   29084947
  REMARK    GeneRIF: Data show that pleckstrin homology containing family
            member 5 (Plekhg5)-depleted cultured motoneurons show defective
            axon growth and impaired autophagy of synaptic vesicles, which can
            be rescued by constitutively active ras-related protein Rab-26
            (Rab26).
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3842)
  AUTHORS   Iwatake,M., Nishishita,K., Okamoto,K. and Tsukuba,T.
  TITLE     The Rho-specific guanine nucleotide exchange factor Plekhg5
            modulates cell polarity, adhesion, migration, and podosome
            organization in macrophages and osteoclasts
  JOURNAL   Exp Cell Res 359 (2), 415-430 (2017)
   PUBMED   28847484
  REMARK    GeneRIF: Plekhg5 affects cell polarity, migration, adhesion,
            degradation, and podosome organization in macrophages and
            osteoclasts.
REFERENCE   5  (bases 1 to 3842)
  AUTHORS   Wu,C., Agrawal,S., Vasanji,A., Drazba,J., Sarkaria,S., Xie,J.,
            Welch,C.M., Liu,M., Anand-Apte,B. and Horowitz,A.
  TITLE     Rab13-dependent trafficking of RhoA is required for directional
            migration and angiogenesis
  JOURNAL   J Biol Chem 286 (26), 23511-23520 (2011)
   PUBMED   21543326
REFERENCE   6  (bases 1 to 3842)
  AUTHORS   Goh,L.L. and Manser,E.
  TITLE     The RhoA GEF Syx is a target of Rnd3 and regulated via a Raf1-like
            ubiquitin-related domain
  JOURNAL   PLoS One 5 (8), e12409 (2010)
   PUBMED   20811643
  REMARK    GeneRIF: a well defined Rnd3 effector Syx
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 3842)
  AUTHORS   Ernkvist,M., Luna Persson,N., Audebert,S., Lecine,P., Sinha,I.,
            Liu,M., Schlueter,M., Horowitz,A., Aase,K., Weide,T., Borg,J.P.,
            Majumdar,A. and Holmgren,L.
  TITLE     The Amot/Patj/Syx signaling complex spatially controls RhoA GTPase
            activity in migrating endothelial cells
  JOURNAL   Blood 113 (1), 244-253 (2009)
   PUBMED   18824598
REFERENCE   8  (bases 1 to 3842)
  AUTHORS   Garnaas,M.K., Moodie,K.L., Liu,M.L., Samant,G.V., Li,K., Marx,R.,
            Baraban,J.M., Horowitz,A. and Ramchandran,R.
  TITLE     Syx, a RhoA guanine exchange factor, is essential for angiogenesis
            in Vivo
  JOURNAL   Circ Res 103 (7), 710-716 (2008)
   PUBMED   18757825
REFERENCE   9  (bases 1 to 3842)
  AUTHORS   Liu,M. and Horowitz,A.
  TITLE     A PDZ-binding motif as a critical determinant of Rho guanine
            exchange factor function and cell phenotype
  JOURNAL   Mol Biol Cell 17 (4), 1880-1887 (2006)
   PUBMED   16467373
REFERENCE   10 (bases 1 to 3842)
  AUTHORS   Yoshizawa,M., Sone,M., Matsuo,N., Nagase,T., Ohara,O., Nabeshima,Y.
            and Hoshino,M.
  TITLE     Dynamic and coordinated expression profile of dbl-family guanine
            nucleotide exchange factors in the developing mouse brain
  JOURNAL   Gene Expr Patterns 3 (3), 375-381 (2003)
   PUBMED   12799088
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL611927.21 and AL772240.7.
            
            On Oct 15, 2019 this sequence version replaced XM_006538926.1.
            
            Summary: This gene encodes a protein belonging to the Rho guanine
            exchange factor (GEF) family of proteins, which activate GTPases by
            replacing GDP with GTP. This family member is a RhoA GEF that plays
            a role in endothelial cell migration and tube formation. It is
            required for angiogenesis and may function in neuronal cell
            differentiation. Alternative splicing of this gene results in
            multiple transcript variants. [provided by RefSeq, Oct 2013].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7345562.1772762.1,
                                           SRR7974084.4843.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-104               AL611927.21        88293-88396
            105-181             AL611927.21        89096-89172
            182-311             AL611927.21        103495-103624
            312-414             AL611927.21        104441-104543
            415-475             AL611927.21        104865-104925
            476-567             AL611927.21        105202-105293
            568-704             AL611927.21        105660-105796
            705-853             AL611927.21        106059-106207
            854-1063            AL611927.21        107518-107727
            1064-1255           AL611927.21        107820-108011
            1256-1351           AL611927.21        108357-108452
            1352-1402           AL611927.21        108909-108959
            1403-1552           AL611927.21        109163-109312
            1553-1663           AL611927.21        109427-109537
            1664-1813           AL611927.21        109632-109781
            1814-1951           AL611927.21        109854-109991
            1952-2071           AL611927.21        110129-110248
            2072-2204           AL772240.7         3757-3889
            2205-2320           AL772240.7         3972-4087
            2321-2502           AL772240.7         4168-4349
            2503-3273           AL772240.7         5447-6217
            3274-3842           AL772240.7         6479-7047
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 82.86 cM"
     gene            1..3842
                     /gene="Plekhg5"
                     /gene_synonym="Syx"
                     /note="pleckstrin homology domain containing, family G
                     (with RhoGef domain) member 5"
                     /db_xref="GeneID:269608"
                     /db_xref="MGI:MGI:2652860"
     exon            1..104
                     /gene="Plekhg5"
                     /gene_synonym="Syx"
                     /inference="alignment:Splign:2.1.0"
     CDS             101..3283
                     /gene="Plekhg5"
                     /gene_synonym="Syx"
                     /note="isoform 2 is encoded by transcript variant 3;
                     RhoA-specific guanine exchange factor SYX1; RhoA-specific
                     guanine exchange factor SYX2; synectin-binding RhoA
                     exchange factor; PH domain-containing family G member 5"
                     /codon_start=1
                     /product="pleckstrin homology domain-containing family G
                     member 5 isoform 2"
                     /protein_id="NP_001361712.1"
                     /db_xref="CCDS:CCDS89862.1"
                     /db_xref="GeneID:269608"
                     /db_xref="MGI:MGI:2652860"
                     /translation="MEDQSPAEEKGLRCQNPACMDKGRAAKVCHHADCQQLHHRGPLN
                     LCETCDSKFHSTLHYDGHVRFDLPPQGSVLARNVSTRSCPPRTSPAADLEEEEEGCTD
                     GKGDRKSAGLKISKKKARRRHTDDPSKECFTLKFDLNVDIETEIVPAMKKKSLGEVLL
                     PVFERKGIALGKVDIYLDQSNTPLSLTFEAYRFGGHYLRVKAKPGDEGKVEQGVKDSK
                     SLSLPALRPSGAGPPVSERVDPQSRRESSLDILAPGRRRKNMSEFLGEAGIPGHEPPA
                     PSSCSLPVGSSGGTSSGINESWKNRAASRFSGFFSSSPSTSAFSREVDKMEQLESKLH
                     AYSLFGLPRMPRRLRFDHDSWEEEEEDDEEDEESSGLRLEDSWRELTDGHEKLTRRQC
                     HQQEAVWELLHTEVSYIRKLRVITNLFLCCLLNLQESGLLCEVEAERLFSNIPEIAKL
                     HRGLWGSVMVPVLEKARRTRALLQPSDFLKGFKMFGSLFKPYIRYCMEEEGCMEYMRG
                     LLRDNDLFRAYVTWAEKHQQCQRLKLSDMLAKPHQRLTKYPLLLKSVLRKTDDPRTKE
                     AIVTMISSVERFIHHVNTCMRQRQERQRLAGVVSRIDAYEVVEGSNDEVDKLLKEFLH
                     LDLTAPMPGTSPEETRQLLLEGSLRMKEGKDSKMDVYCFLFTDLLLVTKAVKKAERTK
                     VIRPPLLVDKIVCRELRDPGSFLLIYLNEFHSAVGAYTFQASSQALCRSWVDTIYNAQ
                     NQLQQLRAQLSAQEHPGSQHLQSLEEEEDEQEEEGEESGTSAASSPTILRKSSNSLDS
                     EHCTSDGSTETLAMVVVEPGATLSSPEFEGGPVSSQSDESSLSNTASSVTPTSELLPL
                     GPVDGRSCSMDSAYGTLSPTSLQDFVAPHPVVEPAPVPQTPSPQPSPRLRRRTPVQLL
                     PRPPRLLKSKSEASLLQLLSGTPAARGVPPAPSRSLSELCLISVAPGVRTQRPLQEGG
                     PGWNGPGMCDPCHGPQLSESENRPSHMTGGPADSARRRCREMPSGTMSRVQSEPPSGV
                     SAQHRKLTLAQLYRIRTTLLLNSTLTASEV"
     exon            105..181
                     /gene="Plekhg5"
                     /gene_synonym="Syx"
                     /inference="alignment:Splign:2.1.0"
     exon            182..311
                     /gene="Plekhg5"
                     /gene_synonym="Syx"
                     /inference="alignment:Splign:2.1.0"
     exon            312..414
                     /gene="Plekhg5"
                     /gene_synonym="Syx"
                     /inference="alignment:Splign:2.1.0"
     exon            415..475
                     /gene="Plekhg5"
                     /gene_synonym="Syx"
                     /inference="alignment:Splign:2.1.0"
     exon            476..567
                     /gene="Plekhg5"
                     /gene_synonym="Syx"
                     /inference="alignment:Splign:2.1.0"
     exon            568..704
                     /gene="Plekhg5"
                     /gene_synonym="Syx"
                     /inference="alignment:Splign:2.1.0"
     exon            705..853
                     /gene="Plekhg5"
                     /gene_synonym="Syx"
                     /inference="alignment:Splign:2.1.0"
     exon            854..1063
                     /gene="Plekhg5"
                     /gene_synonym="Syx"
                     /inference="alignment:Splign:2.1.0"
     exon            1064..1255
                     /gene="Plekhg5"
                     /gene_synonym="Syx"
                     /inference="alignment:Splign:2.1.0"
     exon            1256..1351
                     /gene="Plekhg5"
                     /gene_synonym="Syx"
                     /inference="alignment:Splign:2.1.0"
     exon            1352..1402
                     /gene="Plekhg5"
                     /gene_synonym="Syx"
                     /inference="alignment:Splign:2.1.0"
     exon            1403..1552
                     /gene="Plekhg5"
                     /gene_synonym="Syx"
                     /inference="alignment:Splign:2.1.0"
     exon            1553..1663
                     /gene="Plekhg5"
                     /gene_synonym="Syx"
                     /inference="alignment:Splign:2.1.0"
     exon            1664..1813
                     /gene="Plekhg5"
                     /gene_synonym="Syx"
                     /inference="alignment:Splign:2.1.0"
     exon            1814..1951
                     /gene="Plekhg5"
                     /gene_synonym="Syx"
                     /inference="alignment:Splign:2.1.0"
     exon            1952..2071
                     /gene="Plekhg5"
                     /gene_synonym="Syx"
                     /inference="alignment:Splign:2.1.0"
     exon            2072..2204
                     /gene="Plekhg5"
                     /gene_synonym="Syx"
                     /inference="alignment:Splign:2.1.0"
     exon            2205..2320
                     /gene="Plekhg5"
                     /gene_synonym="Syx"
                     /inference="alignment:Splign:2.1.0"
     exon            2321..2502
                     /gene="Plekhg5"
                     /gene_synonym="Syx"
                     /inference="alignment:Splign:2.1.0"
     exon            2503..3273
                     /gene="Plekhg5"
                     /gene_synonym="Syx"
                     /inference="alignment:Splign:2.1.0"
     exon            3274..3842
                     /gene="Plekhg5"
                     /gene_synonym="Syx"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3813..3818
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Plekhg5"
                     /gene_synonym="Syx"
                     /note="hexamer: TATAAA"
     polyA_site      3842
                     /gene="Plekhg5"
                     /gene_synonym="Syx"
                     /note="major polyA site"
ORIGIN      
        1 cccgccccgc ctcgcgcgcc aggcggccgc ggcgctcggt tcccccgctc ccgcgctcgg
       61 tcctcggctc ccgcgctcgg ttcccctgcg gctcggcgcc atggaagacc agagccctgc
      121 agaggagaag ggactgcgct gccagaaccc agcctgcatg gacaagggcc gggccgccaa
      181 ggtatgccat catgccgact gccagcagct gcaccaccgg gggcctctca acctgtgtga
      241 gacctgtgat agcaagttcc acagcaccct gcactacgac ggccatgtcc gatttgacct
      301 gcccccgcaa ggctctgtcc tggcccggaa tgtttccacc cggtcctgtc ctccccgcac
      361 cagccctgct gcagacctgg aggaagaaga ggaaggctgt accgacggaa aaggggaccg
      421 gaagagcgcc ggcctaaaaa tctctaagaa gaaagcaagg aggagacaca cagatgaccc
      481 gagcaaggag tgcttcacct tgaaattcga cctgaacgtg gacatcgaaa cagaaattgt
      541 gccagccatg aagaagaagt cactggggga agtactgctg cctgtgtttg agaggaaggg
      601 catcgcactg ggcaaggtag acatctacct ggatcaatcc aacacacccc tgtccctcac
      661 cttcgaggcc tacaggtttg gaggacacta cctgcgggtc aaagccaagc cgggggacga
      721 gggcaaggtg gagcagggag tgaaggattc caagtccctc agtctgccag ccctgaggcc
      781 cagtggggct gggccccctg tgtcggaacg tgtggaccct cagagccgcc gagagagcag
      841 tctggacatc ttggcccctg gccgccgtcg caagaacatg tctgaattcc tgggagaggc
      901 aggcatccct ggacacgagc cccccgcacc ttccagctgt tctctgcccg ttggcagcag
      961 tgggggtacc agcagtggca tcaatgagag ctggaagaac cgggcagcca gtcgcttcag
     1021 cggcttcttc agctccagcc cgagcaccag tgcctttagc cgggaggtag acaagatgga
     1081 acagctggag agcaagctcc atgcctacag ccttttcggg ctaccccgga tgcccaggag
     1141 gctgcgcttt gaccatgact cctgggagga ggaagaggaa gatgacgagg aggatgaaga
     1201 gagttcaggc ctgaggctgg aggacagctg gagggagctc actgatgggc atgagaagct
     1261 gaccaggcgg cagtgccacc aacaggaggc agtgtgggaa ctcctccaca cagaagtctc
     1321 ctacatccgg aaactacgtg tgatcaccaa cctgttcctg tgttgtcttc ttaacctaca
     1381 agagtcgggg ctcctgtgtg aggttgaggc tgagcgcttg ttcagcaaca ttcctgagat
     1441 cgccaagcta caccgcggct tgtggggcag cgtgatggtg ccagtgctgg agaaggcgcg
     1501 gcggacgcgg gcgctgctgc agcccagcga ctttctcaaa ggcttcaaga tgttcggctc
     1561 actcttcaag ccatacatcc gatactgtat ggaagaggag ggctgcatgg agtacatgcg
     1621 cggcctgctg cgtgacaacg atctgttccg ggcatacgtc acgtgggcgg agaagcacca
     1681 gcagtgccag cggctgaagc tgagtgacat gctggccaag ccccaccagc ggctcaccaa
     1741 atacccactg ctgctcaagt cggtgctaag gaagacagat gatccccgca ccaaggaagc
     1801 catcgtcacc atgatcagct cggtggaacg cttcatacac cacgtgaaca cgtgcatgcg
     1861 gcagcggcag gagcggcagc gcctggcggg ggtggtgagc cggatcgatg cctacgaggt
     1921 tgtggagggc agcaacgacg aggtggataa gctcctgaag gaatttctac atctggacct
     1981 gacagcaccc atgcctggaa cctcccctga agagacccga cagctgctgc tggaagggag
     2041 cctgaggatg aaggagggga aagatagcaa gatggacgtg tactgcttcc tcttcactga
     2101 cctactcttg gtgaccaagg ccgtgaagaa agcagagagg acgaaggtca tcaggccacc
     2161 gctactggtg gacaagattg tgtgccggga acttcgggac cctggttcct tcctcctcat
     2221 ctacctgaat gaattccaca gtgctgtggg ggcctacact ttccaggcca gcagccaggc
     2281 cttgtgccgg agctgggtgg acactattta caacgcacag aaccagctgc agcagctgcg
     2341 tgcacagctc agtgcacagg agcacccagg cagccagcat ctgcagagcc tggaagagga
     2401 ggaagatgag caggaggagg agggtgaaga gagcggtacc tcagctgcca gctcccccac
     2461 catccttcga aagagcagca acagcctcga ctctgagcac tgcacctcag atggctccac
     2521 ggagaccctg gccatggttg tggtagagcc tggggcgaca ctgtcttccc cggagtttga
     2581 aggcggtccc gtcagctccc agtcggacga atcttctctc agtaacactg cttcctctgt
     2641 cactcccacc agcgagctgc tgcccctggg ccctgtggat ggccgctcct gctccatgga
     2701 ctccgcctat ggcaccctgt cccccacttc ccttcaagac tttgtggctc cacaccccgt
     2761 ggtcgagcca gcgcctgtgc ctcagacccc ctctccccag ccctcacccc gcctccgccg
     2821 tcgcactcct gtccagctgc tccctcgccc gccccgcctg ctcaagtcca aatcggaggc
     2881 tagcctctta caattactgt caggaacccc agccgcccgt ggcgtgcctc cggctcccag
     2941 ccgcagcctg tcagaactgt gcctgatctc tgtggcccct ggggttagga ctcagcgccc
     3001 ccttcaggaa ggtggtcctg gctggaatgg tccagggatg tgtgacccct gccatggccc
     3061 tcagttgtca gaatctgaga acagacctag ccacatgacg gggggacctg cagattctgc
     3121 ccgaaggaga tgcagagaga tgccttctgg gactatgtcc agggtgcagt ctgagcctcc
     3181 ctcaggggtc tctgctcaac acaggaagct gaccctggcc cagctctacc ggatcaggac
     3241 caccctgctg ctcaactcca cgctcactgc ctcggaggtg tgagcaggag acccctaggg
     3301 tgccactgaa taagagacag tagagagccc tgtcctctgg ggatacagca cctgcttcac
     3361 cgctgcctcg tgtgtgtgaa gaccaaggag acccccaagg gtaccactgc ctgaggacag
     3421 cagagagaac ctgcctccct cccagaagtc acagcacctg cttctcagct cctcatgtgt
     3481 gcatgtgtgc cagagcgcca ggcagggtgg ttgcctcatc acctcatggc caatttgcac
     3541 tttgccagac tggatggaat agagaagggt cctgtggagt tccaggccct ggctgcaatg
     3601 ctccacacac acacacaccc ctcttgccca ggtggcctct tgtcctctgc tcttgactct
     3661 gccagccctg attttctggg ctgggctcac tggctctcaa tgccaccttc gttaggattc
     3721 atgaaatgag tccctgtcct tcctggtcat gctggcctcc gaggcgcggg ctgggatctg
     3781 tcaggctgcg tatttattaa gtttggctat tctataaaga cttgtatata cttccgtgga
     3841 aa
//
