LOCUS       NM_024413               1559 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus pleckstrin homology domain containing, family F (with
            FYVE domain) member 1 (Plekhf1), mRNA.
ACCESSION   NM_024413
VERSION     NM_024413.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Li,T., Qin,K., Li,N., Han,C. and Cao,X.
  TITLE     An endosomal LAPF is required for macrophage endocytosis and
            elimination of bacteria
  JOURNAL   Proc Natl Acad Sci U S A 116 (26), 12958-12963 (2019)
   PUBMED   31189603
  REMARK    GeneRIF: Study identified LAPF (lysosome-associated and
            apoptosis-inducing protein containing PH and FYVE domains) as a
            Cav1-interacting protein by mass spectrometry and find that LAPF is
            critically involved in inducing innate immune responses and in
            enhancing bacterial endocytosis and the bactericide capacity of
            macrophages by inducing Src-LAPF-Caveolin complex formation.
REFERENCE   3  (bases 1 to 1559)
  AUTHORS   Bailey,P.J., Klos,J.M., Andersson,E., Karlen,M., Kallstrom,M.,
            Ponjavic,J., Muhr,J., Lenhard,B., Sandelin,A. and Ericson,J.
  TITLE     A global genomic transcriptional code associated with CNS-expressed
            genes
  JOURNAL   Exp Cell Res 312 (16), 3108-3119 (2006)
   PUBMED   16919269
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AW227287.1 and AK143976.1.
            
            On Mar 21, 2008 this sequence version replaced NM_024413.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AW227287.1, AK143976.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-12                AW227287.1         1-12
            13-1559             AK143976.1         2-1548
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 25.36 cM"
     gene            1..1559
                     /gene="Plekhf1"
                     /gene_synonym="1810013P09Rik; APPD; LAPF; PHAFIN1;
                     ZFYVE15"
                     /note="pleckstrin homology domain containing, family F
                     (with FYVE domain) member 1"
                     /db_xref="GeneID:72287"
                     /db_xref="MGI:MGI:1919537"
     exon            1..54
                     /gene="Plekhf1"
                     /gene_synonym="1810013P09Rik; APPD; LAPF; PHAFIN1;
                     ZFYVE15"
                     /inference="alignment:Splign:2.1.0"
     exon            55..1559
                     /gene="Plekhf1"
                     /gene_synonym="1810013P09Rik; APPD; LAPF; PHAFIN1;
                     ZFYVE15"
                     /inference="alignment:Splign:2.1.0"
     CDS             71..910
                     /gene="Plekhf1"
                     /gene_synonym="1810013P09Rik; APPD; LAPF; PHAFIN1;
                     ZFYVE15"
                     /note="lysosome-associated apoptosis-inducing protein
                     containing PH and FYVE domains; PH domain-containing
                     family F member 1"
                     /codon_start=1
                     /product="pleckstrin homology domain-containing family F
                     member 1"
                     /protein_id="NP_077724.2"
                     /db_xref="CCDS:CCDS21158.1"
                     /db_xref="GeneID:72287"
                     /db_xref="MGI:MGI:1919537"
                     /translation="MVDHLANTEINSQRIAAVESCFGASGQPLALPGRVLLGEGVLTK
                     ECRKKAKPRIFFLFNDILVYGSIVLSKRKYRSQHIIPLEEVTLEPLPETLQAKNRWMI
                     KTAKKSFVVSAASTTERQEWISHIEECVRRQLLATGRQPTTEHAAPWIPDKATDICMR
                     CTQTRFSALTRRHHCRKCGFVVCAECSRERFLLPRLSPKPLRVCSLCYRELAAQKLRE
                     EAREGIGGSPPQLSHLGGTVCGASSGDDDDSDEDREGNGDGDWPTQVEFYASGVSWSA
                     FHS"
     regulatory      1518..1523
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Plekhf1"
                     /gene_synonym="1810013P09Rik; APPD; LAPF; PHAFIN1;
                     ZFYVE15"
                     /note="hexamer: AATAAA"
     regulatory      1528..1533
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Plekhf1"
                     /gene_synonym="1810013P09Rik; APPD; LAPF; PHAFIN1;
                     ZFYVE15"
                     /note="hexamer: AATAAA"
     polyA_site      1536
                     /gene="Plekhf1"
                     /gene_synonym="1810013P09Rik; APPD; LAPF; PHAFIN1;
                     ZFYVE15"
                     /note="major polyA site"
     polyA_site      1540
                     /gene="Plekhf1"
                     /gene_synonym="1810013P09Rik; APPD; LAPF; PHAFIN1;
                     ZFYVE15"
                     /note="major polyA site"
ORIGIN      
        1 ttggcgggcg ccagttcggg actggctgtg cggacgcgga ggttcctacg ggagcttagc
       61 tattgagaag atggtggacc acttggcaaa cacggagatc aacagccagc ggattgcagc
      121 agtggagagc tgttttgggg catcggggca accgctggcc ctgcccggcc gtgtgctcct
      181 aggcgagggg gtactgacca aggagtgccg caagaaggcc aagccacgca tcttctttct
      241 tttcaacgac atcctggtgt atggcagtat tgtgcttagc aagcgcaagt accgcagcca
      301 gcatattatt cccctagaag aggtaacatt ggagccactg cccgagaccc tgcaggccaa
      361 gaaccgatgg atgatcaaga cagccaagaa gtcctttgtg gtgtcggcag cctccaccac
      421 ggagcgccag gaatggataa gccacatcga agagtgtgtg cggaggcagc tactggccac
      481 aggccgccag cccaccacgg agcacgcagc tccctggatc ccggacaagg ccacggacat
      541 ttgcatgcgc tgcacgcaga cgcgcttctc cgcactcacc cggcgtcacc actgccgcaa
      601 atgcgggttt gtggtctgtg ctgagtgttc cagggagcgt ttcctgttgc cacgtctctc
      661 tcccaagccc cttcgcgtct gcagcctgtg ctaccgagag ctggccgccc agaagctgag
      721 ggaggaggcc agggagggga tcggaggctc tccaccgcag ctgtcccacc taggcggcac
      781 tgtctgtggt gcatccagtg gcgatgacga tgactccgat gaggacagag agggcaatgg
      841 agatggtgac tggcccaccc aggtggagtt ctatgcttct ggtgtctcct ggtcagcctt
      901 ccatagttga cctttacctg cagagtgttt gtatcaagtc agtcctctgc cggagaccct
      961 tgcccatggc agtgggcgtg tgacaggtgg ctgttccaga gccattacta gtgcctttct
     1021 actagactgt agcctcctgg gccctcttct aagtcttttt cagataattt cctttccatt
     1081 tcacatgcag tccttggtga ccaggctata gctgcaaccc ttggaacttg accaggccag
     1141 gtgccccacg cattaagggt taaagagggc aaagggaagc ttgggtttca gagtccattc
     1201 ccaccttcaa ccatgaacag gtacagccca tggaagagcc tgagacttgc acatctacca
     1261 tgcagatatc accacctggc aaccgggtcc ttccaaactg gacgccagat gtacggtgtg
     1321 cgcccttcag gcccaggtga cctacagcct gagctctctg agctcaagcc agcccatcaa
     1381 gccctctgcc ctgtcactac caaggtgcct ggtgcccatt tggcttgtcc ccatctctgt
     1441 ctgcggttaa tagaatcagt gttttctcgt taggactcta gggtcaggga gaaaagccct
     1501 atccaaactg atttaccaat aaacaagaat aaagtaccaa ctcttttgat tgttaagag
//
