LOCUS       NM_145516               3317 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus pleckstrin homology domain containing, family B
            (evectins) member 2 (Plekhb2), transcript variant 1, mRNA.
ACCESSION   NM_145516
VERSION     NM_145516.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Chatterjee,S., Nandakumar,P., Auer,D.R., Gabriel,S.B. and
            Chakravarti,A.
  TITLE     Gene- and tissue-level interactions in normal gastrointestinal
            development and Hirschsprung disease
  JOURNAL   Proc Natl Acad Sci U S A 116 (52), 26697-26708 (2019)
   PUBMED   31818953
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Krappa,R., Nguyen,A., Burrola,P., Deretic,D. and Lemke,G.
  TITLE     Evectins: vesicular proteins that carry a pleckstrin homology
            domain and localize to post-Golgi membranes
  JOURNAL   Proc Natl Acad Sci U S A 96 (8), 4633-4638 (1999)
   PUBMED   10200314
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CK625615.1, AK039771.1, AK149868.1 and AV382273.1.
            
            On Dec 19, 2006 this sequence version replaced NM_145516.1.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1). Both variants 1 and 3 encode the same isoform.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DT922812.1, SRR1660815.66054.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-33                CK625615.1         1-33
            34-355              AK039771.1         2-323
            356-1468            AK149868.1         316-1428
            1469-3307           AK039771.1         1437-3275
            3308-3317           AV382273.1         204-213
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 13.34 cM"
     gene            1..3317
                     /gene="Plekhb2"
                     /gene_synonym="2310009M15Rik; evt-2; Evt2; Phdc"
                     /note="pleckstrin homology domain containing, family B
                     (evectins) member 2"
                     /db_xref="GeneID:226971"
                     /db_xref="MGI:MGI:2385825"
     exon            1..105
                     /gene="Plekhb2"
                     /gene_synonym="2310009M15Rik; evt-2; Evt2; Phdc"
                     /inference="alignment:Splign:2.1.0"
     exon            106..150
                     /gene="Plekhb2"
                     /gene_synonym="2310009M15Rik; evt-2; Evt2; Phdc"
                     /inference="alignment:Splign:2.1.0"
     CDS             114..779
                     /gene="Plekhb2"
                     /gene_synonym="2310009M15Rik; evt-2; Evt2; Phdc"
                     /note="isoform 1 is encoded by transcript variant 1;
                     pleckstrin homology domain-containing family B member 2;
                     PH domain-containing family B member 2; evectin-2"
                     /codon_start=1
                     /product="pleckstrin homology domain-containing family B
                     member 2 isoform 1"
                     /protein_id="NP_663491.1"
                     /db_xref="CCDS:CCDS14874.1"
                     /db_xref="GeneID:226971"
                     /db_xref="MGI:MGI:2385825"
                     /translation="MAFVKSGWLLRQSTILKRWKKNWFDLWSDGHLIYYDDQTRQSIE
                     DKVHMPVDCINIRTGHECRDIQPPDGKPRDCLLQIVCRDGKTISLCAESTDDCLAWKF
                     TLQDSRTNTAYVGSAILSEETAVAASPPPYAAYATPTPEVYGYGPYSGAYPAGTQVVY
                     AANGQAYAVPYQYPYAGVYGQQPANQVIIRERYRDNDSDLALGMLAGAATGMALGSLF
                     WVF"
     exon            151..303
                     /gene="Plekhb2"
                     /gene_synonym="2310009M15Rik; evt-2; Evt2; Phdc"
                     /inference="alignment:Splign:2.1.0"
     exon            304..406
                     /gene="Plekhb2"
                     /gene_synonym="2310009M15Rik; evt-2; Evt2; Phdc"
                     /inference="alignment:Splign:2.1.0"
     exon            407..446
                     /gene="Plekhb2"
                     /gene_synonym="2310009M15Rik; evt-2; Evt2; Phdc"
                     /inference="alignment:Splign:2.1.0"
     exon            447..536
                     /gene="Plekhb2"
                     /gene_synonym="2310009M15Rik; evt-2; Evt2; Phdc"
                     /inference="alignment:Splign:2.1.0"
     exon            537..642
                     /gene="Plekhb2"
                     /gene_synonym="2310009M15Rik; evt-2; Evt2; Phdc"
                     /inference="alignment:Splign:2.1.0"
     exon            643..3317
                     /gene="Plekhb2"
                     /gene_synonym="2310009M15Rik; evt-2; Evt2; Phdc"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3290..3295
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Plekhb2"
                     /gene_synonym="2310009M15Rik; evt-2; Evt2; Phdc"
                     /note="hexamer: ATTAAA"
     polyA_site      3312
                     /gene="Plekhb2"
                     /gene_synonym="2310009M15Rik; evt-2; Evt2; Phdc"
                     /note="major polyA site"
ORIGIN      
        1 cggtgacgca ggaccaggac tcgcgcgtcc agcggagaag caggagaagc cggcgacctt
       61 gcgctctcag cctgatccct gtcttggcgg cctgaacatt cgcagctgga gagatggcgt
      121 tcgtgaagag tggatggtta cttcggcaga gcaccattct gaaacgctgg aagaagaatt
      181 ggttcgacct gtggtcagac ggtcacctga tctactacga tgatcagact cggcagagca
      241 tagaggataa ggtccacatg cccgtggact gcatcaatat ccgcacgggg catgagtgcc
      301 gggacatcca gcctccagat gggaagccca gagactgtct gctgcagatc gtttgccgag
      361 acgggaagac catcagtctc tgtgcagaga gcacagacga ttgcctggca tggaagttta
      421 cactgcagga ttccagaaca aacacagctt acgttggttc agcaatcctg tctgaagaga
      481 ctgcagtggc cgcgtccccg cctccctacg cagcctatgc tacaccaacc cctgaggtct
      541 acggctatgg tccatacagc ggcgcatacc ccgcaggaac tcaagttgtc tatgccgcca
      601 acgggcaggc atatgcagtg ccataccagt acccgtatgc aggagtttat ggacaacagc
      661 ctgccaacca agtcatcatc cgcgagcggt accgagacaa tgacagtgac ctggctctgg
      721 gcatgctcgc cggggcagcc accggcatgg ccctgggctc tctgttctgg gtcttctaga
      781 gccttcaaca ttttctgtgc atagcttctg ttagtcctgt gtgcagtaat ttgatttgca
      841 gggcatttct gtttgtgaca agtgtctttc ataataattt aaatagttct tttgaaggtg
      901 gtaatctaat aattgtgact gacctgcatg gtaccacaaa gaaagcccga ggtatgctgt
      961 gagtgagagc ctgagtcctt ccgggtacta gcttgcacca agtctttctt agggactttt
     1021 ggatggcttt atgtaaacac acccagttaa atgggcaatt tccgtccagt taggtgcagt
     1081 gttgaattaa gggatggctt tccttgctat gccaatacta atactgctga tggaggaaga
     1141 tgtgtgcaag tgtggtgagg agagtcacag cttctttaac tgtggattct cttctagacc
     1201 cctgctgcgt gttaccctag gagctgtggg ctggtggctc ctgcaagact atggtgtgag
     1261 gaccctgtaa cgtacctctt ggagcactta ggtacccctt gaagctccta ggtatcacca
     1321 gcaggattgg ctgctcagga tgcagagggc caccccctcc ctttaaaaat tacgctccag
     1381 taatctgccc agttttattt tcttgttatt cttctgtttg cttttcctgg ggatgattgg
     1441 cattagtctg gagttaggaa ttgattcgag tgccggtggg tggaggcatg cagggagctg
     1501 cccagcgacc tgctctcagt gtttgtttta ggtatattga ttgccagctc aggctgcaga
     1561 gagcctatag agactatttt tctacttgta aagaaagtat ggtgagggga attgaggaga
     1621 gccttgtttg aatgttcctg cctcaggcct cctggggccc acactgcgtg gtcctgggga
     1681 ggcttttcct cagcagtgga agggaggccc cgtggctgtc cagagtctca ggttttgagt
     1741 gagaaatggg attgggtaga gcatctcagg gatttgttct aatccctcat gttatgggga
     1801 tccagccgtg ttctcagtcc agacccgctc acctcagaag agcttaaaac atttctggtc
     1861 cccaaatgtg tggcactctg agaagctcac aatctggctt tctaacgaaa atttgtattt
     1921 ctaaaattag agaatacatg ttccacgcat ttaaaattta tgttctttca tgttttaaag
     1981 ctcccaaatc cagctttgtg actggcatat tttagtttca aacagtaccc cggcacaaag
     2041 gtgggatggc acagtgaagg ccccccgccc tctactttgc atagtcttgt ttctccaggg
     2101 tgctcccagg aagcattcat tctgactttg ctcagcccag tgcatgcgtg ctgccttgcc
     2161 gccgtgctgc tgggtagctc tttcttggtc agatcaagtc ttcaacagat ctccatgtga
     2221 gacagttgcc aagtagatga ggtggtgccc atagtgcttt ctcgatactc cttggggacc
     2281 tgttgacacc tgcccatttc cagctgacat ttgtttttct gtcatctctg atagatggga
     2341 tatgtgacaa catggtacgg acgccgttca gtgtcgcttt aataagcatg atgctgattt
     2401 tacatcctgt gctgtatgac tgccatttgc tcacagtgtc accattgcta aagctccgtg
     2461 ctttacttac aaaacactaa aaccagtggt tagtgtttca cagtgatttt aattttagag
     2521 ttagttactg gcattcctaa agccatagag tactgagtca catccctgaa gtacttttga
     2581 aacagaattg tctcctactg tcccatgggt gtgccctgcc tgtctcctgg ccccaatggg
     2641 gctagctgta ccaggcagcc atagttgagc ctgatcattc ctgtcaccag tttgacttga
     2701 ttatataccc agaatggaat acattcttgg gcatctcagt tcctcagccc tgatcctcat
     2761 agacgccacc ctttcgatgg cttttgcggc gtcacttgta cctcagtgag tcctgcgatt
     2821 cttgagttag agggaacgac ttgtccagca ttgaggaaca tgtctcctcc actgagactt
     2881 aaatgatgat gcagggctgg aagaggctgg ctgctgacac tgcatcgtgg ctgatgtcat
     2941 tgctctccta gttctttgat ttaagaacct ttcatatgga aggcctgagg ctccctcaga
     3001 tcgtcccttg ccaagaaggc ctggcttagg tcattagtgc ccacagtagc cttctggagt
     3061 gtagcaagtt cctgcgtttg agacagaatg gttcagattt attttctaca tctgttgttg
     3121 accccatgca ccctctcatt ttgccttcca gtctacgtag atgaaagatg aaaggcagag
     3181 gatgcagaca gtcttctttg tgattgcttc tgttattctg ttgcatctac cgagcccgtt
     3241 ttctccctgt ctgtgcatac agtatgttta taagtgaact tgttaaaata ttaaatgatc
     3301 actaacctta aaccgtg
//
