LOCUS       NM_019549               4117 bp    mRNA    linear   ROD 03-JUN-2024
DEFINITION  Mus musculus pleckstrin (Plek), mRNA.
ACCESSION   NM_019549
VERSION     NM_019549.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Ray,J.P., Staron,M.M., Shyer,J.A., Ho,P.C., Marshall,H.D.,
            Gray,S.M., Laidlaw,B.J., Araki,K., Ahmed,R., Kaech,S.M. and
            Craft,J.
  TITLE     The Interleukin-2-mTORc1 Kinase Axis Defines the Signaling,
            Differentiation, and Metabolism of T Helper 1 and Follicular B
            Helper T Cells
  JOURNAL   Immunity 43 (4), 690-702 (2015)
   PUBMED   26410627
REFERENCE   2  (bases 1 to 4117)
  AUTHORS   Abdallah,B.M., Ditzel,N., Mahmood,A., Isa,A., Traustadottir,G.A.,
            Schilling,A.F., Ruiz-Hidalgo,M.J., Laborda,J., Amling,M. and
            Kassem,M.
  TITLE     DLK1 is a novel regulator of bone mass that mediates estrogen
            deficiency-induced bone loss in mice
  JOURNAL   J Bone Miner Res 26 (7), 1457-1471 (2011)
   PUBMED   21308776
REFERENCE   3  (bases 1 to 4117)
  AUTHORS   Meuillet,E.J.
  TITLE     Novel inhibitors of AKT: assessment of a different approach
            targeting the pleckstrin homology domain
  JOURNAL   Curr Med Chem 18 (18), 2727-2742 (2011)
   PUBMED   21649580
  REMARK    GeneRIF: Targeting of the pleckstrin homology domain of AKT was
            studied in cancer treatment in mouse models.
            Review article
REFERENCE   4  (bases 1 to 4117)
  AUTHORS   Lian,L., Wang,Y., Flick,M., Choi,J., Scott,E.W., Degen,J.,
            Lemmon,M.A. and Abrams,C.S.
  TITLE     Loss of pleckstrin defines a novel pathway for PKC-mediated
            exocytosis
  JOURNAL   Blood 113 (15), 3577-3584 (2009)
   PUBMED   19190246
  REMARK    GeneRIF: pleckstrin regulates the fusion of granules to the cell
            membrane and is an essential component of PKC-mediated exocytosis
            in platelets
REFERENCE   5  (bases 1 to 4117)
  AUTHORS   Cmarik,J.L., Hegamyer,G., Gerrard,B., Dean,M. and Colburn,N.H.
  TITLE     cDNA cloning and mapping of mouse pleckstrin (Plek), a gene
            upregulated in transformation-resistant cells
  JOURNAL   Genomics 66 (2), 204-212 (2000)
   PUBMED   10860665
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY149522.1, AK151460.1 and CJ198197.1.
            
            On Jul 17, 2009 this sequence version replaced NM_019549.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK156235.1, AF073294.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN02415133 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-225               BY149522.1         2-226
            226-3873            AK151460.1         138-3785
            3874-4117           CJ198197.1         160-403
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 9.41 cM"
     gene            1..4117
                     /gene="Plek"
                     /gene_synonym="2010300B13Rik"
                     /note="pleckstrin"
                     /db_xref="GeneID:56193"
                     /db_xref="MGI:MGI:1860485"
     exon            1..162
                     /gene="Plek"
                     /gene_synonym="2010300B13Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             121..1173
                     /gene="Plek"
                     /gene_synonym="2010300B13Rik"
                     /codon_start=1
                     /product="pleckstrin"
                     /protein_id="NP_062422.1"
                     /db_xref="CCDS:CCDS24446.1"
                     /db_xref="GeneID:56193"
                     /db_xref="MGI:MGI:1860485"
                     /translation="MEPKRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSP
                     KGMIPLKGSTLTSPCQDFGKRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKAIKC
                     IEGGQKFARKSTRRSIRLPETIDLGALYLSMKDPEKGIKELNLEKDKKVFNHCLTGSG
                     VIDWLVSNKLVRNRQEGLMISASLLSEGYLQPAGDLSKNAADGIAENPFLDSPDAFYY
                     FPDSGFFCEENSSDDDVILREEFRGVIIKQGCLLKQGHRRKNWKVRKFILREDPAYLH
                     YYDPAGGEDPLGAVHLRGCVVTSVESSHDVKKSDEENLFEIITADEVHYYLQAATSKE
                     RTEWIKAIQVASRTGK"
     exon            163..318
                     /gene="Plek"
                     /gene_synonym="2010300B13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            319..500
                     /gene="Plek"
                     /gene_synonym="2010300B13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            501..592
                     /gene="Plek"
                     /gene_synonym="2010300B13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            593..777
                     /gene="Plek"
                     /gene_synonym="2010300B13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            778..882
                     /gene="Plek"
                     /gene_synonym="2010300B13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            883..966
                     /gene="Plek"
                     /gene_synonym="2010300B13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            967..1036
                     /gene="Plek"
                     /gene_synonym="2010300B13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1037..1169
                     /gene="Plek"
                     /gene_synonym="2010300B13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1170..4117
                     /gene="Plek"
                     /gene_synonym="2010300B13Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3842..3847
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Plek"
                     /gene_synonym="2010300B13Rik"
                     /note="hexamer: AATAAA"
     polyA_site      3870
                     /gene="Plek"
                     /gene_synonym="2010300B13Rik"
                     /note="major polyA site"
ORIGIN      
        1 agaagagaaa ttgtgaaaga gacctccctg taaaaagggc agcccacaaa cacctcagcc
       61 ttcagaggag actgagctcc tcagacgaca agagtggacc cgtgtgtctc cccaccaggc
      121 atggaaccaa agcggatcag ggagggatac cttgtgaaga agggaagtgt gtttaatacc
      181 tggaaaccca tgtgggttgt actgttagaa gatggaattg agttttataa aaagaaaagt
      241 gacaatagcc ccaaggggat gatcccgctg aaaggaagta ctctcactag cccttgtcaa
      301 gactttggca aacggatgtt cgtgttaaaa atcactacaa ccaaacagca ggaccacttc
      361 ttccaggcag ccttcctgga ggagagagac gcttgggttc gggatatcaa gaaagccatt
      421 aaatgtattg aaggaggcca gaagtttgca agaaaatcca caagacggtc cattcgcctg
      481 ccagagacaa ttgacttggg ggctttgtat ctgtctatga aagaccccga gaaaggaata
      541 aaggaactga atctggagaa ggacaagaaa gtttttaatc actgcctcac aggtagtggt
      601 gtcattgact ggctggtttc taacaagcta gttcggaatc gccaggaagg cctcatgatc
      661 tccgcttccc tgctcagtga agggtacctg cagcctgctg gagatctgtc caagaatgca
      721 gcagatggca tagctgaaaa ccctttcctg gatagccctg atgctttcta ctactttcca
      781 gacagtggat tcttctgtga ggagaactcc agtgatgatg atgtcattct gagagaagaa
      841 ttcagagggg ttatcatcaa gcagggatgt ttactgaagc aggggcaccg gaggaaaaac
      901 tggaaagtga gaaagttcat tctgagagaa gacccggcct acctgcacta ctatgatcct
      961 gctgggggtg aagatcccct gggagcagtt cacttgagag gctgtgtggt gacttcagta
     1021 gagagcagtc acgatgttaa gaagagtgat gaagagaacc tctttgagat catcacagca
     1081 gatgaagttc attattactt gcaggcagcc acttccaagg agcgtactga gtggatcaaa
     1141 gccatccagg tggcctcacg gactgggaaa tgaggacacc gagaatcact gaggagcatc
     1201 tgactggcca agattctaca acccatataa cagagatgga attcaaggcc agactttgaa
     1261 ctttgagtcg gcattctgtt ttgtcttcac cttcctcaca tgcttttggg gtgcatcatt
     1321 ttaatgtgtt gtttatttaa tattcactga agcaagagtt ctcaagcttt atgggtctgt
     1381 tccccacaca ccagcactca aatcaaacaa tattgtgctt gtataacttt ctatgggaag
     1441 tctagagact ttacagatag tccagaaagt ctcaaggctg gaggagcatg aggcctttca
     1501 tccctttgta ttctgagttc tccttcctgt tttcttctca cctgaataaa acaattacca
     1561 tgacacttca caccacacat aagacatcag cctacaagtg tccattgctc ttctgccctt
     1621 ctgctcaatg tcatttctcc aatgaacaag aatgtcagct gagtccttag aaaaatctga
     1681 gcctgagata gagcaaggag tgaatgaatt gtattaaaaa ggggaaagaa tgatactaaa
     1741 atacacaaaa gtgctaggaa ctagaattag gtgaggtaac ttcaacgcaa gtttaactac
     1801 gggtttggcc tggaaagcat agcaaggggt catctttccc cgaccaaaca ggacatcagg
     1861 tcttctgtct tgtgtggaga agtgactcaa agtgacaata tatttatggt ctaatgaaca
     1921 gtaatgtatg ctctctctaa ttgttcagag tcttataagg aaaaaagaca gaaagatcag
     1981 agacaaggga ctcttgggaa gaagcataag gatggacatc tgggagtggg tgtaaagtgt
     2041 gaggatgtgt gtgtcacatt ctaataatcc cagaagcttc catccccaaa ccacttggcc
     2101 tgtaatcacc atagccctag cacaaatgac acatgaatgg ttacccatag gtaaccattg
     2161 gtatatcaca gtggtggata tagaggtcat atacagggac aacagcattg gcttcaactt
     2221 tccaacctta tttagctatt gccacctctg agtgtccagc acatgactag tgaagaccag
     2281 tgctgaacac tgtgtggtat tcttcaagga aatgaacaag ttatttgata agttggctgt
     2341 atttcattct gaatactttg tcctcataag acagatgctg aatctagctg tggatttctg
     2401 tttcttattt agagtattct atttatagac aagggtccac atattttaac gtttagagag
     2461 ttattgtaga tgggaaaagg aaatagaccc attttttatg ggtgtagcac atctttcaat
     2521 cccttaatcc ctcaatcaca ttatctcctt gaagtgctgc ctagctcacc agttaccaat
     2581 tcttaattca tgttaagaac atccttcttg tatattcaca tttctgtgtt tagatcagaa
     2641 ccctaccatt tactgtgtag attcatttca agagcttcca aatagacttc cataccccat
     2701 aactatactc caatctactt tatatatatt tatcactaaa ctctatatgg aatagaggaa
     2761 ccttaaatat ataatgttgc ttatcttcca aactttagct catctccaat gtttccattg
     2821 cttgtaccat agaatctaaa gttggtgaac tgtaaggtta ataatgttaa tttgactaaa
     2881 ctgggagatg cttaatgtgt agggtatcag tttagcacat atgtaaatat gtctgtaagg
     2941 agaattttaa aagataatta attcatgata gctctgactc agtgaatgga tagttgatcc
     3001 attggtagat tcaagactat taggaggtgg tggaactttg caagttggag gaagaaggtg
     3061 ggtgtatcct tccggaggta tatctgggct tctcctgtta tcatcactct gcaatgattc
     3121 ctgtctgcct taatgtgccc tgccctggtt cactacactc aatgactgtg gaggactgaa
     3181 acgcttaaat ttgtgaactg aaaacaactt cttctaagtt atttctgatg gtcattttgt
     3241 cacagcatca aacaaagcac ataaactcgt acataagcaa gggatgtcct tattggtcaa
     3301 acattaattt tcataaaatc caggagtaag atgggaaact taatataatc ttataatgaa
     3361 acagaaaagt aaacaaatag ctggaaaggc aagatgtgtc cactggtggc atagggacat
     3421 tacttgtagg ggtgtaacca atggctttct cataggatct gaggcctact ctctggaaga
     3481 caattcatgt actattggtc aaatgcctgt gaatagggag gacataagcc ctgtcaggaa
     3541 tggtatttct attgttttgc taaatattca tgtcctgcct atcaaattgc ccttgaaaag
     3601 acctctagtt tcatgagtcc caaagctaag aatgtcctca gacaaaattc aatgcctact
     3661 ttgctgtaat ccttataacc tatatgatgt ttctaccaat gtatttggga aaaaaatgcc
     3721 ttgatttagg actttgttct gttactgtga aaatgcacgt aagtgatgca acactgtagt
     3781 agggtttttg ggaaagttca atctatattc ccaagccata gtcactcata ttttgctcta
     3841 gaataaactc taaactttta ttcctttgaa ggtaagagct gtcttttttg tgtcaatggt
     3901 gtggaagccc ctaacccttc tatggagaac acccatgtct ccatctgggt gttcctcaat
     3961 ggaatcttgt ttgtttgttt gttttgagac agggtttctc tgtatcgccc tggctgtcct
     4021 gggactcact ttgtagacca ggctggcctc gatctcagaa atctgcctgc ctctgcctcc
     4081 caagaggatc ttttataata aactggcatt tgtacag
//
