LOCUS       NM_001163540           15455 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus plectin (Plec), transcript variant 13, mRNA.
ACCESSION   NM_001163540
VERSION     NM_001163540.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 15455)
  AUTHORS   Rezniczek,G.A., Walko,G. and Wiche,G.
  TITLE     Plectin gene defects lead to various forms of epidermolysis bullosa
            simplex
  JOURNAL   Dermatol Clin 28 (1), 33-41 (2010)
   PUBMED   19945614
  REMARK    Review article
            Erratum:[Dermatol Clin. 2010 Apr;28(2):439-41]
REFERENCE   2  (bases 1 to 15455)
  AUTHORS   Winter,L., Abrahamsberg,C. and Wiche,G.
  TITLE     Plectin isoform 1b mediates mitochondrion-intermediate filament
            network linkage and controls organelle shape
  JOURNAL   J Cell Biol 181 (6), 903-911 (2008)
   PUBMED   18541706
  REMARK    GeneRIF: Plectin 1b both forms a mitochondrial signaling platform
            and affects organelle shape and network formation by tethering
            mitochondria to intermediate filaments.
REFERENCE   3  (bases 1 to 15455)
  AUTHORS   Sonnenberg,A. and Liem,R.K.
  TITLE     Plakins in development and disease
  JOURNAL   Exp Cell Res 313 (10), 2189-2203 (2007)
   PUBMED   17499243
  REMARK    Review article
REFERENCE   4  (bases 1 to 15455)
  AUTHORS   Rezniczek,G.A., Konieczny,P., Nikolic,B., Reipert,S., Schneller,D.,
            Abrahamsberg,C., Davies,K.E., Winder,S.J. and Wiche,G.
  TITLE     Plectin 1f scaffolding at the sarcolemma of dystrophic (mdx) muscle
            fibers through multiple interactions with beta-dystroglycan
  JOURNAL   J Cell Biol 176 (7), 965-977 (2007)
   PUBMED   17389230
  REMARK    GeneRIF: We propose a model in which plectin acts as a universal
            mediator of desmin intermediate filament anchorage at the
            sarcolemma and Z-disks.
REFERENCE   5  (bases 1 to 15455)
  AUTHORS   Zhang,T., Haws,P. and Wu,Q.
  TITLE     Multiple variable first exons: a mechanism for cell- and
            tissue-specific gene regulation
  JOURNAL   Genome Res 14 (1), 79-89 (2004)
   PUBMED   14672974
REFERENCE   6  (bases 1 to 15455)
  AUTHORS   Rezniczek,G.A., Abrahamsberg,C., Fuchs,P., Spazierer,D. and
            Wiche,G.
  TITLE     Plectin 5'-transcript diversity: short alternative sequences
            determine stability of gene products, initiation of translation and
            subcellular localization of isoforms
  JOURNAL   Hum Mol Genet 12 (23), 3181-3194 (2003)
   PUBMED   14559777
REFERENCE   7  (bases 1 to 15455)
  AUTHORS   Andra,K., Kornacker,I., Jorgl,A., Zorer,M., Spazierer,D., Fuchs,P.,
            Fischer,I. and Wiche,G.
  TITLE     Plectin-isoform-specific rescue of hemidesmosomal defects in
            plectin (-/-) keratinocytes
  JOURNAL   J Invest Dermatol 120 (2), 189-197 (2003)
   PUBMED   12542521
  REMARK    GeneRIF: Expression of plectin 1a, but not of its N-terminal
            fragment alone, or of a third alternative full length isoform
            (plectin 1), restored the reduced number of hemidesmosome-like
            stable anchoring contacts in cultured plectin-null keratinocytes
REFERENCE   8  (bases 1 to 15455)
  AUTHORS   Leung,C.L., Green,K.J. and Liem,R.K.
  TITLE     Plakins: a family of versatile cytolinker proteins
  JOURNAL   Trends Cell Biol 12 (1), 37-45 (2002)
   PUBMED   11854008
  REMARK    Review article
REFERENCE   9  (bases 1 to 15455)
  AUTHORS   Fuchs,P., Zorer,M., Rezniczek,G.A., Spazierer,D., Oehler,S.,
            Castanon,M.J., Hauptmann,R. and Wiche,G.
  TITLE     Unusual 5' transcript complexity of plectin isoforms: novel
            tissue-specific exons modulate actin binding activity
  JOURNAL   Hum Mol Genet 8 (13), 2461-2472 (1999)
   PUBMED   10556294
REFERENCE   10 (bases 1 to 15455)
  AUTHORS   Wiche,G.
  TITLE     Role of plectin in cytoskeleton organization and dynamics
  JOURNAL   J Cell Sci 111 (Pt 17), 2477-2486 (1998)
   PUBMED   9701547
  REMARK    Review article
REFERENCE   11 (bases 1 to 15455)
  AUTHORS   Elliott,C.E., Becker,B., Oehler,S., Castanon,M.J., Hauptmann,R. and
            Wiche,G.
  TITLE     Plectin transcript diversity: identification and tissue
            distribution of variants with distinct first coding exons and
            rodless isoforms
  JOURNAL   Genomics 42 (1), 115-125 (1997)
   PUBMED   9177781
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AF188008.1, AY480033.1,
            CN669707.1 and AI464512.1.
            
            Summary: Plectin is a prominent member of an important family of
            structurally and in part functionally related proteins, termed
            plakins or cytolinkers, that are capable of interlinking different
            elements of the cytoskeleton. Plakins, with their multi-domain
            structure and enormous size, not only play crucial roles in
            maintaining cell and tissue integrity and orchestrating dynamic
            changes in cytoarchitecture and cell shape, but also serve as
            scaffolding platforms for the assembly, positioning, and regulation
            of signaling complexes (reviewed in PMID: 9701547, 11854008,
            17499243). Plectin is expressed as several protein isoforms in a
            wide range of cell types and tissues from a single gene located on
            chromosome 8 in humans (PMID: 8633055, 8698233). Until 2010, this
            locus was named plectin 1 (symbol PLEC1 in human; Plec1 in mouse
            and rat) and the gene product had been referred to as
            'hemidesmosomal protein 1' or 'plectin 1, intermediate filament
            binding 500kDa'. These names were superseded by plectin. The
            plectin gene locus in mouse on chromosome 15 has been analyzed in
            detail (PMID: 10556294, 14559777), revealing a genomic exon-intron
            organization with well over 40 exons spanning over 62 kb and an
            unusual 5' transcript complexity of plectin isoforms. Eleven exons
            (1-1j) have been identified that alternatively splice directly into
            a common exon 2 which is the first exon to encode plectin's highly
            conserved actin binding domain (ABD). Three additional exons (-1,
            0a, and 0) splice into an alternative first coding exon (1c), and
            two additional exons (2alpha and 3alpha) are optionally spliced
            within the exons encoding the acting binding domain (exons 2-8).
            Analysis of the human locus has identified eight of the eleven
            alternative 5' exons found in mouse and rat (PMID: 14672974); exons
            1i, 1j and 1h have not been confirmed in human. Furthermore,
            isoforms lacking the central rod domain encoded by exon 31 have
            been detected in mouse (PMID:10556294), rat (PMID: 9177781), and
            human (PMID: 11441066, 10780662, 20052759). It has been shown that
            the short alternative amino-terminal sequences encoded by the
            different first exons direct the targeting of the various isoforms
            to distinct subcellular locations (PMID: 14559777). As the
            expression of specific plectin isoforms was found to be dependent
            on cell type (tissue) and stage of development (PMID: 10556294,
            12542521, 17389230) it appears that each cell type (tissue)
            contains a unique set (proportion and composition) of plectin
            isoforms, as if custom-made for specific requirements of the
            particular cells. Concordantly, individual isoforms were found to
            carry out distinct and specific functions (PMID: 14559777,
            12542521, 18541706). In 1996, a number of groups reported that
            patients suffering from epidermolysis bullosa simplex with muscular
            dystrophy (EBS-MD) lacked plectin expression in skin and muscle
            tissues due to defects in the plectin gene (PMID: 8698233, 8941634,
            8636409, 8894687, 8696340). Two other subtypes of plectin-related
            EBS have been described: EBS-pyloric atresia (PA) and EBS-Ogna. For
            reviews of plectin-related diseases see PMID: 15810881, 19945614.
            Mutations in the plectin gene related to human diseases should be
            named based on the position in NM_000445 (human variant 1, isoform
            1c), unless the mutation is located within one of the other
            alternative first exons, in which case the position in the
            respective Reference Sequence should be used. [provided by RefSeq,
            Aug 2011].
            
            Transcript Variant: This variant (13), also known as 12alpha,
            differs in the 5' UTR and the 5' coding region, and contains an
            additional in-frame coding exon compared to variant 1. The
            resulting protein (isoform 12alpha) contains a distinct N-terminus
            and contains a 5 aa internal protein segment compared to isoform
            1c. This variant (13) contains the same 5' coding exon as variant 6
            but also contains an alternate in-frame exon so the resulting
            protein contains a 5aa internal segment compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMN00849374, SAMN00849375
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: PMID: 18541706, 17389230
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3075              AF188008.1         1-3075
            3076-7786           AY480033.1         2542-7252
            7787-7867           CN669707.1         56-136
            7868-15453          AY480033.1         7334-14919
            15454-15455         AI464512.1         1-2                 c
FEATURES             Location/Qualifiers
     source          1..15455
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 35.48 cM"
     gene            1..15455
                     /gene="Plec"
                     /gene_synonym="EBS1; PCN; Plec1; PLTN"
                     /note="plectin"
                     /db_xref="GeneID:18810"
                     /db_xref="MGI:MGI:1277961"
     exon            1..724
                     /gene="Plec"
                     /gene_synonym="EBS1; PCN; Plec1; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            725..786
                     /gene="Plec"
                     /gene_synonym="EBS1; PCN; Plec1; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            787..801
                     /gene="Plec"
                     /gene_synonym="EBS1; PCN; Plec1; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            802..891
                     /gene="Plec"
                     /gene_synonym="EBS1; PCN; Plec1; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            892..969
                     /gene="Plec"
                     /gene_synonym="EBS1; PCN; Plec1; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            970..1062
                     /gene="Plec"
                     /gene_synonym="EBS1; PCN; Plec1; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1063..1229
                     /gene="Plec"
                     /gene_synonym="EBS1; PCN; Plec1; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1230..1345
                     /gene="Plec"
                     /gene_synonym="EBS1; PCN; Plec1; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1346..1452
                     /gene="Plec"
                     /gene_synonym="EBS1; PCN; Plec1; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1453..1572
                     /gene="Plec"
                     /gene_synonym="EBS1; PCN; Plec1; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1573..1668
                     /gene="Plec"
                     /gene_synonym="EBS1; PCN; Plec1; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1669..1796
                     /gene="Plec"
                     /gene_synonym="EBS1; PCN; Plec1; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1797..1890
                     /gene="Plec"
                     /gene_synonym="EBS1; PCN; Plec1; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            1891..2045
                     /gene="Plec"
                     /gene_synonym="EBS1; PCN; Plec1; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            2046..2355
                     /gene="Plec"
                     /gene_synonym="EBS1; PCN; Plec1; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            2356..2433
                     /gene="Plec"
                     /gene_synonym="EBS1; PCN; Plec1; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            2434..2595
                     /gene="Plec"
                     /gene_synonym="EBS1; PCN; Plec1; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            2596..2700
                     /gene="Plec"
                     /gene_synonym="EBS1; PCN; Plec1; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            2701..2796
                     /gene="Plec"
                     /gene_synonym="EBS1; PCN; Plec1; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            2797..2922
                     /gene="Plec"
                     /gene_synonym="EBS1; PCN; Plec1; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            2923..3075
                     /gene="Plec"
                     /gene_synonym="EBS1; PCN; Plec1; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            3076..3230
                     /gene="Plec"
                     /gene_synonym="EBS1; PCN; Plec1; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            3231..3357
                     /gene="Plec"
                     /gene_synonym="EBS1; PCN; Plec1; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            3358..3541
                     /gene="Plec"
                     /gene_synonym="EBS1; PCN; Plec1; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            3542..3699
                     /gene="Plec"
                     /gene_synonym="EBS1; PCN; Plec1; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            3700..3878
                     /gene="Plec"
                     /gene_synonym="EBS1; PCN; Plec1; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            3879..4017
                     /gene="Plec"
                     /gene_synonym="EBS1; PCN; Plec1; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            4018..4374
                     /gene="Plec"
                     /gene_synonym="EBS1; PCN; Plec1; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            4375..4458
                     /gene="Plec"
                     /gene_synonym="EBS1; PCN; Plec1; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            4459..4563
                     /gene="Plec"
                     /gene_synonym="EBS1; PCN; Plec1; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            4564..4662
                     /gene="Plec"
                     /gene_synonym="EBS1; PCN; Plec1; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            4663..8043
                     /gene="Plec"
                     /gene_synonym="EBS1; PCN; Plec1; PLTN"
                     /inference="alignment:Splign:2.1.0"
     exon            8044..15455
                     /gene="Plec"
                     /gene_synonym="EBS1; PCN; Plec1; PLTN"
                     /inference="alignment:Splign:2.1.0"
     regulatory      15433..15438
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Plec"
                     /gene_synonym="EBS1; PCN; Plec1; PLTN"
                     /note="hexamer: AATAAA"
     polyA_site      15455
                     /gene="Plec"
                     /gene_synonym="EBS1; PCN; Plec1; PLTN"
                     /note="major polyA site"
ORIGIN      
        1 acagagcaag gctctggagc cgcactgccc ctgagcctgc gcccttgtgc ttagaaccgg
       61 agcctgcctg cctgtttgcc agtggcccct gctgcatctt gtgccaaagg agcttcatca
      121 gcctcctggc tgtgccccac ctgctgtcct caccctgtgc tgaccggcag gcttccagca
      181 gccatggtgg ctggcatgct catgccgctg gaccggctac gggccatcta tgaggtgctc
      241 ttccgtgagg gggtgatggt tgccaagaag gaccggcgac cccgcagcct gcatccccat
      301 gtgcccggcg tcaccaatct acaggtcatg cgtgccatgg cttcactgaa agctcggggc
      361 ctggtgcggg agacctttgc ctggtgccac ttctactggt acctgaccaa cgagggcatt
      421 gaccacctac gccagtactt acacctgcca ccggagattg tacctgcctc tctgcagcgt
      481 gtgcgccgcc ctgtcgccat ggtgatacct gcacgtcgtc gctcccccca tgtgcagacc
      541 atgcaaggtc ccctaggctg cccaccaaag agggggcctc tgccagctga ggaccctgcc
      601 cgtgaggagc ggcaggtcta tcgcaggaag gagcgtgagg agggggcacc tgaaacccct
      661 gtggtgtctg ccaccaccgt ggggaccctg gccagaccag gtccggagcc tgccccagcc
      721 acagatgaac gagaccgtgt gcagaagaaa actttcacca agtgggtcaa caaacacctt
      781 atcaagcact ggagggcaga ggctcagagg cacatcagtg acctgtacga agacctccgt
      841 gatggccaca acctcatctc cctgctggaa gtcctctcag gagacagcct gccccgcgag
      901 aaagggagga tgcgcttcca caagctgcag aatgtgcaga ttgccctgga ctatctccga
      961 catcgccagg tgaagttagt gaacatcaga aatgatgaca ttgctgacgg caaccccaag
     1021 ctgaccctcg gcctcatctg gacaatcatc ctgcacttcc agatctcaga cattcaggtg
     1081 agcggacagt cggaggacat gacagcaaag gagaagctgc tgctgtggtc acagcgtatg
     1141 gtagagggct accaaggcct gcgctgtgac aacttcacca ccagttggcg cgatggccgt
     1201 ctcttcaatg ctatcatcca ccggcacaag cccatgctca tagatatgaa taaagtgtat
     1261 cgacagacca acctggagaa cctagaccag gcattctcgg tggcagagcg ggacctggga
     1321 gttacacggc tcctggaccc agaagatgtg gacgtccctc agcctgatga aaagtccatc
     1381 atcacctatg tttcatccct gtacgatgct atgccccgcg tgcctggtgc acaggacgga
     1441 gtgagggcca atgagctgca gcttcgttgg caggagtacc gggagcttgt gttgctgctg
     1501 ctgcaatgga tccggcacca caccgctgct tttgaggagc gcaagttccc ctccagcttt
     1561 gaagagattg agatcctatg gtgccagttt ttgaagttca aggagacaga acttcctgcc
     1621 aaggaggcag acaaaaaccg gtccaaagtt atctaccagt ctttggaggg ggcagtacaa
     1681 gcgggccagc tcaagattcc ccctggctac cacccactag atgtggagaa ggagtggggc
     1741 aagctgcatg tggccatcct ggagcgggag aagcaactgc gaagcgagtt tgagaggctg
     1801 gagtgtcttc agcgcattgt gagcaagcta cagatggagg ctgggctgtg tgaggagcag
     1861 ctgaaccagg cggacgccct actgcagtcg gatattcggc tgctggcctc aggcaaggtg
     1921 gctcagcgag ctggggaagt cgagagagac ctggacaagg ctgatggtat gatccggctg
     1981 ttgttcaatg atgtgcagac ccttaaagat gggcggcatc cacagggtga acagatgtac
     2041 cggagggtgt atcgtctgca tgagcgcctg gtagccatcc gcactgagta caacctccgg
     2101 ctgaaggcag gagtgggtgc ccctgtgacc caggtgaccc tgcagagtac acagaggcgc
     2161 ccagagctag aggactccac actgcgctac ctgcaagacc tgctggcctg ggtagaggag
     2221 aaccagcgtc gaatagacag tgctgagtgg ggcgtggact tgcccagtgt ggaggcccag
     2281 ctgggcagcc accgaggcat gcatcagtct atagaggaat ttcgggccaa gatcgagcgg
     2341 gctcggaatg atgagagcca gctctcccct gccacccggg gtgcctaccg ggactgccta
     2401 ggtcgcctag acctgcagta tgcaaagctg ctgaactcct ccaaggcccg cctccggtcc
     2461 ctggagagct tgcacgggtt tgtggcggca gctaccaagg agctgatgtg gctgaatgag
     2521 aaagaagagg aggaagtggg ctttgattgg agtgaccgca ataccaacat ggcagccaag
     2581 aaagaaagtt actcggccct gatgcgagaa ctggaaatga aggaaaagaa aatcaaggag
     2641 atccagaaca caggggacag gctgctgcgg gaagaccatc ctgcccggcc cacggtggag
     2701 tccttccagg ctgccttgca gacacagtgg agctggatgc tgcagctgtg ttgctgcatt
     2761 gaagcacact tgaaagagaa tacagcctac ttccagttct tctcagatgt tcgggaggcg
     2821 gaggaacagt tgcagaagct acaggagacg ttacgcagga agtacagctg tgaccgcacc
     2881 atcaccgtca ccaggctcga ggatctgctg caggatgccc aggatgagaa ggaacaactg
     2941 aatgagtaca aggggcacct ctcaggcctg gccaagcggg ccaaggctat tgtgcagttg
     3001 aagccacgca accctgccca cccggtgcgg ggccacgtgc ccctgatagc cgtgtgtgac
     3061 tacaagcagg tggaggtgac tgtgcacaag ggtgaccaat gccagctggt gggtcctgcc
     3121 cagccatccc actggaaggt gctcagtggt tccagcagcg aggccgctgt gccttctgtg
     3181 tgctttcttg tgccgccgcc caaccaggag gcccaggaag ctgtcgctag actggaggcg
     3241 cagcatcagg ccctggtcac actgtggcac cagcttcacg tggacatgaa gagccttctg
     3301 gcgtggcaga gcctcagtcg ggacatacag ctcatccggt cctggtccct agtcacgttc
     3361 cgcacactga agcccgagga gcagcgacaa gctctgcgca acctggaatt gcactaccag
     3421 gccttccttc gagacagcca ggatgccggt ggctttgggc ctgaggaccg tctagtggca
     3481 gagcgcgagt atggatcttg cagccgccat taccagcagt tactacaaag cctggagcag
     3541 ggtgagcagg aggagtcccg ctgtcagcga tgcatctcgg agctcaagga tatccggctg
     3601 cagctggagg cctgtgagac tcggactgtg caccgtctgc ggctgccact ggataaggac
     3661 cccgcacggg agtgcgccca gcgcatcgct gagcaacaga aagcgcaggc tgaggtggag
     3721 gggctgggca agggagttgc ccggctgtct gctgaggctg agaaagttct agccttgcca
     3781 gagccgtcac ctgctgcacc cactctgcgc tcggagttgg aactgaccct gggcaagctg
     3841 gaacaggtcc gaagcctgtc tgccatctac ctggagaaac tcaagaccat cagcttggtg
     3901 attcgcagta cccagggggc tgaggaggtg cttaaaaccc atgaggagca gctgaaggag
     3961 gcccaggctg tgcctgccac tctccaagag cttgaagcca ccaaggcctc actaaagaag
     4021 ctgcgggccc aggccgaggc acagcagcca gtattcaaca ccctaagaga tgagttgcgg
     4081 ggggcacagg aggttggtga gcggctacag cagcggcatg gtgagcggga cgtagaagtt
     4141 gagcgctggc gagaacgtgt cactcagttg ctggagcgct ggcaggctgt tctagcccag
     4201 acagatgtgc ggcagcgtga gctcgagcag ctgggccgcc agcttcgcta ctatcgtgag
     4261 agcgcggatc ctctgagcgc ctggctgcag gatgccaaga ggcggcaaga gcagatccag
     4321 gccgtgccaa tagccaactg tcaggctgcg cgagaacagc tgcgccagga gaaggccctg
     4381 ctggaggaga ttgagcgtca tggtgagaag gttgaggagt gccagaagtt tgctaagcag
     4441 tacatcaatg caatcaagga ctatgagctc cagctgatca cgtacaaggc tcagcttgaa
     4501 cccgtggcct cccctgccaa gaagcccaag gttcagtctg gatcggagag cgtcatccag
     4561 gagtacgtgg atctgcgcac gcgctacagc gagctgacca cgctcacgag ccagtatatc
     4621 aagttcatca gtgagaccct tcgccgcatg gaagaggagg agcggctggc tgagcaacag
     4681 cgggcagagg agcgggagcg actggccgag gtggaggctg cgctggagaa gcagcggcag
     4741 ctagctgagg cacacgccca ggctaaggcc caggcagagc tggaagcaca agaactgcag
     4801 cggcgcatgc aggaggaggt ggcgcggcgc gaggaggcag cagtggacgc acagcagcag
     4861 aagcgcagca tccaagagga gctgcagcac ctgaggcaga gctcggaggc agagatccag
     4921 gccaaggccc agcaggtgga ggctgcagaa cgcagccgga tgcgcattga ggaagagatc
     4981 cgtgtagtac gtctgcagct ggagacaact gagcggcagc gtggaggggc ggagggcgag
     5041 ctgcaggctc tgcgcgctcg ggctgaggag gcagaagcgc agaagcggca ggctcaggag
     5101 gaagctgaac gcttgcgaag gcaggtgcag gatgagagcc agcgcaaacg gcaggcagag
     5161 gcagagctgg ccctgcgtgt gaaggctgaa gctgaggcag cgcgagagaa gcagagggcc
     5221 ctgcaggccc tggatgagct gagactgcag gccgaggagg ccgagcggcg gctgcgccaa
     5281 gccgaggcag agcgggcacg ccaagtgcag gtggccctgg agacggcaca gcgcagtgcg
     5341 gaagtggagc tgcagagcaa gcgggcatcc tttgcagaga agaccgcaca attggagcgc
     5401 actctgcagg aggagcatgt aacagtggcc cagctgcggg aggaggccga gcgacgggca
     5461 cagcagcaag ctgaggctga gcgagcccgg gaggaagctg agcgggagct ggagcgctgg
     5521 cagctgaagg ccaatgaagc actgcggctg cggctgcagg cagaagaggt ggcacagcag
     5581 aagagcctgg cacaggccga tgccgagaag cagaaggaag aggccgagcg ggaagcgcgg
     5641 cggcggggca aggcggagga gcaggctgtg cggcagcggg agctggcgga gcaggagctg
     5701 gagaagcagc ggcagctggc ggagggcacc gcacagcagc gcctggctgc ggagcaagag
     5761 ctgatccgcc tgcgggccga gacagagcaa ggtgagcagc agcggcagct gctggaggaa
     5821 gagctggccc ggctacagca cgaggccacc gcagccacgc agaagcgcca ggagctggag
     5881 gctgaactgg ctaaggtccg ggcagagatg gaggtacttc tggccagcaa ggcgcgagcc
     5941 gaggaagagt ctcgctctac cagcgaaaag tctaagcaga ggctagaggc tgaggcaggc
     6001 cgctttcggg aactggccga ggaggctgcc cggctgcgtg ctctggcgga ggaggccaag
     6061 aggcagaggc agttggcaga ggaggatgcc gcccgccagc gggccgaggc agagcgggtg
     6121 cttactgaga agctggctgc catcagtgag gccacacggc tcaagacgga ggcagagatt
     6181 gcactcaaag agaaggaggc cgagaatgag cgcctgaggc gcctggcaga agacgaggcc
     6241 ttccagcggc ggcgtctgga ggagcaggcg gcactgcaca aggctgacat cgaggagcgc
     6301 ctggcccagc tgcgcaaggc atcagagagc gagctggagc ggcagaaggg cttggtggag
     6361 gacaccctgc gacagcggcg gcaggtggag gaggagatca tggcgctgaa ggtgagcttt
     6421 gagaaagccg ccgcgggcaa ggcagagctg gagctggagc tggggcgcat tcgcagcaac
     6481 gcggaggaca caatgcgcag caaggagcag gcggaactgg aggcggcccg gcagcggcag
     6541 ttggcggcag aggaggagca gaggcgccgg gaggccgagg agcgcgtgca gaggagcctg
     6601 gcagcggagg aggaagccgc tcggcagcgc aaggtggcac tggaggaggt cgagcgactc
     6661 aaggccaagg ttgaagaagc acggcgcctg cgagagcggg cagagcagga gtcggccagg
     6721 cagctgcagc tggcccagga ggccgcccag aaacggctgc aggcggagga gaaggcacac
     6781 gcctttgtgg tgcagcagcg agaggaggag ctgcagcaga cgcttcagca agagcagaac
     6841 atgctggatc ggctgcggag tgaggcagag gcagcgaggc gagccgccga ggaggcggag
     6901 gaggcccggg agcaggcgga gcgcgaggca gcgcagtctc ggaaacaagt ggaagaggcc
     6961 gagcggctga aacagtcagc agaggagcag gctcaggccc aggctcaggc gcaggcggcc
     7021 gcagagaagc tgcgcaagga agcggagcag gaggcggcgc gtcgggccca ggcagagcag
     7081 gcagcgctga aacagaagca ggcagccgac gcggagatgg agaagcataa aaagtttgca
     7141 gagcagacac tccggcagaa ggcccaggta gagcaggagc tgaccacact gaggctgcag
     7201 ctagaggaga ctgaccacca gaagagcatc ctggatgagg agctgcagcg actaaaggcc
     7261 gaggtaacag aggcagcccg gcagcgcagc caggtagagg aggagctctt ctctgtccgt
     7321 gtgcagatgg aggagctggg caagctcaag gctcgcattg aagctgaaaa ccgggccctc
     7381 atccttcgtg acaaggacaa cacacagcgc ttcctggagg aggaggccga gaagatgaaa
     7441 caggtggccg aggaagcggc acggttgagc gtggccgccc aggaggcagc acggctgagg
     7501 cagctagcgg aggaggacct ggcgcagcag cgggccctgg cagagaagat gctgaaggag
     7561 aagatgcagg cggtgcagga agccacgagg ctcaaggccg aggctgagct gctgcagcag
     7621 cagaaggagc ttgcccagga gcaggcccgg cggctgcagg aggacaagga gcaaatggct
     7681 cagcagttgg tagaggagac acagggtttc cagaggactc tggaggccga gcggcagcgg
     7741 cagctggaga tgagcgcgga ggccgagcgc ctcaagctgc gcatggctga gatgagccgg
     7801 gctcaggccc gcgcggagga ggacgcccag cgcttccgga agcaggctga agagatcggc
     7861 gagaagctgc accgcactga acttgcgaca caggagaagg tgacgttggt gcagacgctg
     7921 gagatccagc gacagcagag tgaccacgat gccgagcgtc tgagggaggc cattgcggag
     7981 ctggagcgtg agaaggagaa gctcaagcag gaggcgaagt tactgcagct caagtcggag
     8041 gagatgcaga ctgtgcagca ggagcagata ctgcaggaga cccaggccct gcagaagagc
     8101 ttcctctctg agaaggacag cctgctgcag cgcgagcgct tcatcgagca ggagaaggcc
     8161 aagctggagc agctcttcca ggacgaggtg gctaaagcaa agcagctgcg tgaggagcag
     8221 cagcggcagc agcagcagat ggaacaggaa aagcaggagc tgatggccag catggaggag
     8281 gccaggaggc ggcagcgcga ggctgaggag ggcgtgaggc gcaagcaaga ggaactgcag
     8341 catctggagc agcagcggca gcagcaggag aagctcctgg cggaggaaaa ccagcggctg
     8401 cgagagcggc tgcagcgcct ggaggaagag caccgcgctg ccttggctca ctctgagatc
     8461 gccaccaccc aggccgcttc cacgaaggca ctgcccaacg gccgggatgc acccgatggc
     8521 ccgtccgtgg aggctgaacc cgagtacacc tttgagggac tacgtcagaa ggtgccggcc
     8581 cagcagctac aggaagcagg catcctgagc caggaggaac tgcagcgttt ggcacagggt
     8641 cacaccacgg tggctgagct cacgcagcgg gaagatgtgt accggtacct gaagggccgc
     8701 agcagcattg caggactgtt actgaagccc accaatgaga aactgagtgt ctacacagcc
     8761 ctacagcggc agctgctcag ccccggaaca gctctgatct tgctggaggc ccaggcagcc
     8821 tcgggcttcc tgctggatcc tgtgcggaat cggcggctga cagtcaatga ggctgtgaag
     8881 gagggtgtcg tgggtcctga gctgcaccac aaactgctgt cagctgagcg tgccgtcacc
     8941 ggctacaagg acccctatac aggggaacag atctcgctct tccaggccat gaagaaggac
     9001 cttattgtca gggaccatgg cgtccgcctg ctggaggccc agatcgccac cggtggcatc
     9061 attgatcctg tgcacagcca ccgtgtgcct gtggacgtgg cctacaaacg tggctacttc
     9121 gatgaagaga tgaaccgcat cttgtctgac ccaagcgatg acaccaaggg cttcttcgac
     9181 cccaacaccc acgagaacct cacttacctg cagctgctgg agcgctgtgt ggaggacccc
     9241 gagaccggcc tgcgcctcct gccactcaca gataaggctg ccaagggtgg cgagctggtg
     9301 tacaccgata cggaggcccg tgacgtcttc gaaaaggcca ccgtgtctgc accgtttggc
     9361 aagttccagg gcaggaccgt gaccatctgg gagatcatca actcggagta cttcacggcg
     9421 gaacagcgac gggacctgct gcagcagttc cgcacgggcc acatcacggt ggagaaaatc
     9481 atcaaaatcg tcatcacagt ggtggaggaa cacgagcgga agggccagct ctgctttgag
     9541 ggcctccgtg ccctcgtgcc cgccgcagag ctgctggaca gtggggtcat cagccacgaa
     9601 ctctaccagc agctgcagcg gggtgagcgt tctgtgcggg aagtggccga ggcggacagc
     9661 gtgaggcagg ccctgcgggg taccaatgtc atcgccggtg tgtggctgga agaagcaggg
     9721 cagaagctga gcatctatga ggccctgaag aaagacttgc tgcagccaga ggtggctgtg
     9781 gccttgctgg aggcccaggc tggcactggg catatcatcg accctgccac cagcgccagg
     9841 ctgactgtgg acgaggcggt gcgtgctggc ctggtggggc ctgagctgca cgagaagctg
     9901 ctgtcagccg agaaggccgt gacaggctac agggatccct actcaggaca gagtgtctca
     9961 ctgttccagg ccttgaagaa gggtctcatc ccccgagaac agggcctgcg cctgctggat
    10021 gcccagttat ccactggtgg cattgtagac cccagcaaaa gccaccgtgt gcccctggat
    10081 gttgcctatg cccggggcta cctggacaaa gagactaaca gggccctgac gtcacccaga
    10141 gatgatgcca gagtctacca tgaccccagc acccaggagc cagtcaccta cagccagctc
    10201 caacagcggt gccggtctga ccagctgacc ggcttgagcc tactgccact ctcagagaag
    10261 gccgtccggg cccggcagga ggaggtctac tctgagctcc aggcccggga gacactggag
    10321 caggccaagg ttgaggttcc tgtgggcagc tttaagggca gggcgatgac tgtgtgggag
    10381 ctcatcagct ctgaatactt tactgaggag cagcggcagg agttgctgcg gcagttccgc
    10441 acaggcaagg tcaccgtgga gaaggtcatc aagattgtca tcaccatcgt ggaggaggtg
    10501 gagactcggc ggcaggagag actgtccttt agtggcctcc gtgcccctgt gccggccagt
    10561 gagctcctgg acgccaagat cctcagcaga gctcagtttg accagctcaa ggatggcaag
    10621 acatcagtca aagagctgtc cgaggtgggc tccgtgcgga ctctgctgca gggcagcggc
    10681 tgcctggccg gcatctatct ggaggactcg aaggagaaag taaccatcta tgaggccatg
    10741 cgccggggcc tcctcagacc cagcacagcc acgctcctgc tggaggccca ggcagccact
    10801 ggtttcctgg tggaccctgt gcggaaccaa cgtctgtatg tccatgaggc tgtcaaggcg
    10861 ggcgtagtgg gccctgaact ccatgagaag ctgctgtcgg ccgagaaggc cgtcactggc
    10921 tacaaggatc cctactcggg caacaccatc tcgctgttcc aggccatgaa aaagggcctg
    10981 gtcctcaggg accatgccat ccgcctgctg gaggcccagg tcgccacggg tggcatcatc
    11041 gacccggtgc acagccaccg cctgcctgtg gacgtggcct accagcgtgg ctacttcgat
    11101 gaggagatga accgtgtgct ggcagatcca agtgatgaca ccaagggctt ctttgacccc
    11161 aacacccacg agaacctcac ctacctgcag ctgctggagc gctgtgtgga ggaccccgag
    11221 accggcctgc gcctcctgcc actcaaaggg gcagagaaga ccgaggtggt agaaaccaca
    11281 caggtgtata ctgaggagga gactcggagg gcgttcgagg agacgcagat tgacatcccg
    11341 ggtggtggca gccacggtgg ctcctccatg tctctgtggg aggtgatgca gtcaaacatg
    11401 atcccggagg atcagcgtgc ccggctcatg gctgactttc aggctgggag ggtaaccaag
    11461 gaacgcatga tcattatcat catcgaaatc atcgagaaga ccgagatcat ccgccagcag
    11521 aacctggcct cctatgacta cgtgcgccgc cgcctcactg ccgaagacct gtacgaggcc
    11581 cggatcatct cccttgagac ctacaacctc ttccgggaag gcaccaagaa cctccgtgaa
    11641 gtgctggaga tggaatctgc ctggcgctac ctttacggca caggcgcggt ggccggtgtc
    11701 tacctgcctg gctccaggca gacgctaacc atctaccagg cccttaagaa ggggttgctg
    11761 agtgccgagg tggcccgctt attgctggaa gcacaggcag ccacgggctt cctgctggac
    11821 ccagtgaagg gggagcggct gaccgtggat gaggctgtgc ggaagggcct ggtaggcccc
    11881 gagctgcatg accgcctcct ctctgccgag cgagctgtaa ctggttaccg agacccctac
    11941 acggaacaga ccatctcact cttccaggcc atgaagaagg agctgatccc tgctgaggag
    12001 gcgctgaggc tgctggatgc gcagctagcc acaggaggca ttgtggaccc ccgcctgggt
    12061 ttccacctcc ctctggaggt ggcttaccaa cgcggctacc tcaataagga cacgcacgac
    12121 cagctgtcgg agcccagcga ggtgcgcagc tacgtggacc cctccacgga tgagcggctc
    12181 agctacacac agctgctcaa gcgttgccgc cgtgatgacc ccagcggcca gatgctgctg
    12241 ctgctctctg atgcccgcaa gctgaccttc cgcggtctcc gcaagcagat cactgtggag
    12301 gagctggtac gctctcaagt catggacgag gccacagcgc tacagctaca agaaggcctg
    12361 acctccatcg aggaggtcac taagaacctg cagaagttcc tcgagggtac cagctgcatt
    12421 gccggagtct ttgttgatgc caccaaggaa cggctctcgg tgtaccaggc tatgaagaag
    12481 ggcatcatcc gccccgggac agcctttgag ctcctggaag cacaggcagc caccggctat
    12541 gtcattgacc ccatcaaggg actcaagctg acggtggaag aagccgttcg tatgggtatt
    12601 gtgggccccg agttcaagga caagctgctg tcagctgagc gtgccgtcac tggctacaag
    12661 gacccctact ctgggaaact catctccctc ttccaggcca tgaagaaggg cctgatcctg
    12721 aaggaccatg gcatccgtct gctggaggct cagatcgcca ctgggggcat cattgacccc
    12781 gaggagagcc accgtttgcc tgtggaagtg gcctataagc gcggcctctt tgatgaggag
    12841 atgaacgaga tcttgactga tccctcagat gacaccaagg gtttctttga ccccaacaca
    12901 gaggagaacc tcacttacct gcagctgatg gagcgctgca tcactgaccc ccagacgggc
    12961 ctgtgtctcc tgcccctgaa ggagaagaag cgggaacgga agacatcctc caagtcctca
    13021 gtgcgcaagc gccgcgtggt gattgtggac ccagagacgg gcaaggagat gtctgtgtac
    13081 gaggcctacc gcaagggtct cattgaccac cagacgtacc tggagttgtc ggagcaggag
    13141 tgtgagtggg aagagatcac catctcctcc tcagatggcg tcgtcaagtc tatgatcatc
    13201 gaccgccgct ctggtcgcca gtatgacatc gacgacgcca tcaccaagaa cctcattgac
    13261 cgctcagcac tggaccagta ccgcgctggt acgctttcga tcactgagtt tgctgacatg
    13321 ctctcaggca atgccggtgg cttccgttcc cgctcctcct ctgtgggctc atcttcctcc
    13381 taccccatca gttctgctgg ccccaggacc cagctagcct cctggtctga tccgactgag
    13441 gagactggcc ccgtggccgg catcctggac acagagactc tggagaaggt gtccatcacg
    13501 gaggccatgc accgcaacct ggtagacaac atcaccggcc agcggctgct ggaggcccaa
    13561 gcctgcaccg ggggcatcat tgaccccagc actggtgagc gcttcccggt cactgaagct
    13621 gtcaacaagg gcctggtgga caagatcatg gtagaccgta tcaatctggc ccagaaggcc
    13681 ttctgtgggt ttgaggaccc acgcaccaag accaagatgt cagctgccca ggccctgaag
    13741 aagggctggc tgtactatga ggcaggccag cgtttcctgg aggtgcagta cctgaccggt
    13801 ggtctgatcg agcctgacac gcccggccgt gtgtctctcg acgaagctct gcaacgtggc
    13861 actgtggatg cccgcacagc ccagaagctg cgtgatgtca gtgcttactc caagtacctc
    13921 acgtgcccca agaccaagct caagatctcc tacaaggacg ctctggaccg tagcatggtg
    13981 gaggagggca cggggctgag gctgctggag gctgcggcac agtccagcaa gggctactac
    14041 agcccgtaca gcgtcagtgg ctctggctcg accgccggct cacgcactgg ttcacgcacc
    14101 ggctccaggg ccggctcccg gcgtggcagc tttgatgcca ctggctctgg cttctccatg
    14161 accttttctt cttcctccta ctcttcctca ggctatggcc gccgctatgc ctcagggcct
    14221 tcagcctctc ttgggggccc tgagtctgca gtggcctgat cccctcagcc tgcatcctgc
    14281 cttcccgctc tgcatgtggc cgggctccct gtggaggcac tggggtcttc tttttttttt
    14341 tctttttttt tcctttttaa catttaaagg tgtcttcctc ccaagcggtg cctaaaatct
    14401 aaccaaaaag accagactaa cacattaata tatgtatgcg atgtccagac agcctgtgtc
    14461 ttggggaaca gggctggccc aggcccagtg accacttcaa tctcctgggt tctccctaaa
    14521 tcctttctac ctgccactca ccacagccag gtgccttgga gaatccagag ctgggcattc
    14581 agcctatacc actcctgtct ctcctgggag gattctatct gggaaaggcc cccagacctc
    14641 taagccaacc ccactggatg tctacctgct ggtcctagct gctgagggga actggggaca
    14701 gtcgtgtgag cagacagctc ttgaggcctc tgtcctgagc cagctgcttc tccccagtct
    14761 atctctgaat gtcctgtata ttcagtgggt tttgctggcc aagggaagat cccaggccaa
    14821 ccatctcttc cagcctaccc agagaagccc cttccccatg ggaagataag gcctggtcct
    14881 ggccccagcc tcccgcctgg ctcctgcagc tgccattgga gctatgcttt gtagctcaac
    14941 accccatact tattcccctg agacctgagc ctctgcttca gccttccagc ctcagctccc
    15001 cttgtaagtg ccttctgtgt ccttgtaccc aggccctaaa gacccagacc cagggtagga
    15061 gatggacatt ctggctgggc ggggctggag ggttctgcag atctgagact tctcctgctc
    15121 cagaggccca gggactcttc aagcctgtgg aacccctctc tgggtgctgc tgcccacctc
    15181 actcccaggg agccctggcc cctccatggc ccagagacac cggctggccc atctatgcta
    15241 acagaagcat ttggccagtg acactctccc ttccttcctg tccagccagt cccgggcctt
    15301 ggtggctcct cccctgcctc caccctctga gtgagctttg catgttccac taaccttgag
    15361 ctggtggcag gtggagatgc caggcagaac actaacctga ccatgggcgg ggccctgcgg
    15421 tgtccgcccc tcaataaaag caattccaac cttca
//
