LOCUS       NM_008876               3703 bp    mRNA    linear   ROD 11-OCT-2024
DEFINITION  Mus musculus phospholipase D2 (Pld2), transcript variant 2, mRNA.
ACCESSION   NM_008876 XM_001471616
VERSION     NM_008876.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Kong,G., Li,D., Liu,X., Feng,J., Ning,F., Huang,X., Qi,B., Qu,J.
            and Wang,X.
  TITLE     PLD2 deficiency alleviates endothelial glycocalyx degradation in
            LPS-induced ARDS/ALI
  JOURNAL   Biochem Biophys Res Commun 716, 150019 (2024)
   PUBMED   38703555
  REMARK    GeneRIF: PLD2 deficiency alleviates endothelial glycocalyx
            degradation in LPS-induced ARDS/ALI.
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Su,Z., Slivka,P., Paulsboe,S., Chu,K., Wetter,J.B., Namovic,M.,
            Perron,D., Kannan,A., Wan,Q., Manning,C., Todorovic,V., Smith,K.M.,
            Lipovsky,A., Wang,Y., Frank,K., McGaraughty,S., Loud,J.,
            Scott,V.E., Honore,P. and Goedken,E.R.
  TITLE     Importance of PLD2 in an IL-23 driven psoriasiform dermatitis model
            and potential link to human psoriasis
  JOURNAL   J Dermatol 50 (10), 1321-1329 (2023)
   PUBMED   37455419
REFERENCE   3  (bases 1 to 3703)
  AUTHORS   Qian,T., Qi,B., Fei,Y., Li,J., Luo,L., Lv,B., Song,Y., Sheng,S.,
            Xiao,W., Huang,X. and Wang,X.
  TITLE     PLD2 deletion alleviates disruption of tight junctions in
            sepsis-induced ALI by regulating PA/STAT3 phosphorylation pathway
  JOURNAL   Int Immunopharmacol 114, 109561 (2023)
   PUBMED   36700766
  REMARK    GeneRIF: PLD2 deletion alleviates disruption of tight junctions in
            sepsis-induced ALI by regulating PA/STAT3 phosphorylation pathway.
REFERENCE   4  (bases 1 to 3703)
  AUTHORS   Kim,H.J., Lee,D.K., Jin,X., Che,X., Ryu,S.H. and Choi,J.Y.
  TITLE     Phospholipase D2 controls bone homeostasis by modulating
            M-CSF-dependent osteoclastic cell migration and microtubule
            stability
  JOURNAL   Exp Mol Med 54 (8), 1146-1155 (2022)
   PUBMED   35945449
REFERENCE   5  (bases 1 to 3703)
  AUTHORS   Cai,M., Wang,Z., Luu,T.T.T., Zhang,D., Finke,B., He,J., Tay,L.W.R.,
            Di Paolo,G. and Du,G.
  TITLE     PLD1 promotes reactive oxygen species production in vascular smooth
            muscle cells and injury-induced neointima formation
  JOURNAL   Biochim Biophys Acta Mol Cell Biol Lipids 1867 (1), 159062 (2022)
   PUBMED   34610470
REFERENCE   6  (bases 1 to 3703)
  AUTHORS   Slaaby,R., Jensen,T., Hansen,H.S., Frohman,M.A. and Seedorf,K.
  TITLE     PLD2 complexes with the EGF receptor and undergoes tyrosine
            phosphorylation at a single site upon agonist stimulation
  JOURNAL   J Biol Chem 273 (50), 33722-33727 (1998)
   PUBMED   9837959
REFERENCE   7  (bases 1 to 3703)
  AUTHORS   Redina,O.E. and Frohman,M.A.
  TITLE     Genomic analysis of murine phospholipase D1 and comparison to
            phospholipase D2 reveals an unusual difference in gene size
  JOURNAL   Gene 222 (1), 53-60 (1998)
   PUBMED   9813240
REFERENCE   8  (bases 1 to 3703)
  AUTHORS   Redina,O.E. and Frohman,M.A.
  TITLE     Organization and alternative splicing of the murine phospholipase
            D2 gene
  JOURNAL   Biochem J 331 (Pt 3) (Pt 3), 845-851 (1998)
   PUBMED   9560313
REFERENCE   9  (bases 1 to 3703)
  AUTHORS   Colley,W.C., Altshuller,Y.M., Sue-Ling,C.K., Copeland,N.G.,
            Gilbert,D.J., Jenkins,N.A., Branch,K.D., Tsirka,S.E., Bollag,R.J.,
            Bollag,W.B. and Frohman,M.A.
  TITLE     Cloning and expression analysis of murine phospholipase D1
  JOURNAL   Biochem J 326 (Pt 3) (Pt 3), 745-753 (1997)
   PUBMED   9307024
REFERENCE   10 (bases 1 to 3703)
  AUTHORS   Colley,W.C., Sung,T.C., Roll,R., Jenco,J., Hammond,S.M.,
            Altshuller,Y., Bar-Sagi,D., Morris,A.J. and Frohman,M.A.
  TITLE     Phospholipase D2, a distinct phospholipase D isoform with novel
            regulatory properties that provokes cytoskeletal reorganization
  JOURNAL   Curr Biol 7 (3), 191-201 (1997)
   PUBMED   9395408
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL592547.9, AK143930.1 and
            AW047839.1.
            
            On Oct 23, 2014 this sequence version replaced NM_008876.2.
            
            Summary: This gene is a member of the phospholipase D (PLD)
            superfamily. The encoded protein catalyzes the hydrolysis of
            phosphatidylcholine to phosphatidic acid and choline. Phosphatidic
            acid is an essential intracellular lipid second messenger for many
            signaling pathways and has been implicated in a variety of
            physiological processes including cytoskeletal organization and
            cell proliferation. A similar gene in human may also function as a
            guanine nucleotide exchange factor (GEF) for the small GTPase Rac2.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Oct 2014].
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the 3' coding region, compared to variant 1. This
            results in a shorter protein (isoform 2), compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK143930.1, U87557.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-9                 AL592547.9         94357-94365         c
            10-816              AK143930.1         2-808
            817-817             AL592547.9         92733-92733         c
            818-3692            AK143930.1         810-3684
            3693-3703           AW047839.1         8-18                c
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 42.99 cM"
     gene            1..3703
                     /gene="Pld2"
                     /gene_synonym="PLD1C"
                     /note="phospholipase D2"
                     /db_xref="GeneID:18806"
                     /db_xref="MGI:MGI:892877"
     exon            1..343
                     /gene="Pld2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            344..453
                     /gene="Pld2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     CDS             345..3146
                     /gene="Pld2"
                     /gene_synonym="PLD1C"
                     /EC_number="3.1.4.4"
                     /note="isoform 2 is encoded by transcript variant 2;
                     choline phosphatase 2; phosphatidylcholine-hydrolyzing
                     phospholipase D2"
                     /codon_start=1
                     /product="phospholipase D2 isoform 2"
                     /protein_id="NP_032902.1"
                     /db_xref="CCDS:CCDS24953.1"
                     /db_xref="GeneID:18806"
                     /db_xref="MGI:MGI:892877"
                     /translation="MTVTQKNLFPYGDYLNSSQLHMEPDEVDTLREGEDPADRMHPYL
                     AIYDLQPLKAHPLVFAPGVPVIAQVVGTERYTSGSKVGTCTLYSVRLTHGDFTWTTKK
                     KFRHFQELHRDLQRHKVLMSLLPLARFAVTHSPAREAAAEDIPSLPRGGSEGSARHTA
                     SKQKYLENYLNRLLTMSFYRNYHAMTEFLEVSQLSFIPDLGSKGLEGVIRKRSGGHRV
                     PGFTFCGRDQVCYRWSKRWLVVKDSFLLYMRPETGAISFVQLFDPGFEVQVGKRSTET
                     RYGVRIDTSHRSLILKCSSYRQARWWGQEITELAQGSGRDFLQLHQHDSYAPPRPGTL
                     ARWFVNGAGYFAAVADAILRAQEEIFITDWWLSPEIYLKRPAHSDDWRLDIMLKRKAE
                     EGVRVSILLFKEVELALGINSGYSKRTLMLLHPNIKVMRHPDLVTLWAHHEKLLVVDQ
                     VVAFLGGLDLAFGRWDDVQYRLTDLGDPSEPVHLQTPTLGSDPAATPDLSHNQFFWLG
                     KDYSNLITKDWVQLDRPFEDFIDRETTPRMPWRDVGVVVHGVAARDLARHFIQRWNFT
                     KTTKARYKTPLYPYLLPKSTSTANNLPFMIPGGQCATVQVLRSVDRWSAGTLENSILN
                     AYLHTIRESQHFLYIENQFFISCSDGRTVLNKVGDEIVDRILKAHEQGQCFRVYLLLP
                     LLPGFEGDISTGGGNSIQAILHFTYRTLCRGEHSILHRLKAAMGTAWRDYMSICGLRT
                     HGELGGHPISELIYIHSKMLIADDRTVIIGSANINDRSLLGKRDSELAILIKDTEMEP
                     SLMDGVEYQAGRFALSLRKHCFSVILGANTWPDLDLRDPVCDDFFQLWQETAENNATI
                     YEQIFRCLPSNATRSLRALREYVAVESLATVSPSLAQSELAHIQGHLVHFPLKFLEDE
                     SLLPPLGSKEGMIPLEVWT"
     exon            454..584
                     /gene="Pld2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            585..727
                     /gene="Pld2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            728..833
                     /gene="Pld2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            834..899
                     /gene="Pld2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            900..958
                     /gene="Pld2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            959..1051
                     /gene="Pld2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            1052..1204
                     /gene="Pld2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            1205..1354
                     /gene="Pld2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            1355..1438
                     /gene="Pld2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            1439..1517
                     /gene="Pld2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            1518..1628
                     /gene="Pld2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            1629..1799
                     /gene="Pld2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            1800..1926
                     /gene="Pld2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            1927..2045
                     /gene="Pld2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            2046..2159
                     /gene="Pld2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            2160..2264
                     /gene="Pld2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            2265..2353
                     /gene="Pld2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            2354..2467
                     /gene="Pld2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            2468..2517
                     /gene="Pld2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            2518..2652
                     /gene="Pld2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            2653..2806
                     /gene="Pld2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            2807..2921
                     /gene="Pld2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     exon            2922..3693
                     /gene="Pld2"
                     /gene_synonym="PLD1C"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3675..3680
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pld2"
                     /gene_synonym="PLD1C"
                     /note="hexamer: AATAAA"
     polyA_site      3693
                     /gene="Pld2"
                     /gene_synonym="PLD1C"
                     /note="major polyA site"
ORIGIN      
        1 ctctggggtg taggctcctg gctgagttct gcctgcttgc gaacccgcac gggcgccggg
       61 gtccgcatcc agggtgcggg ggcccgggac gtggcgcggc tgaggtccgg agccccggga
      121 accgggcggg gacgctgtcg ggctggagct gcaacggcct tctgactcag ccgctcgtcc
      181 agatttcggg atctctctcc tgggcgggga gcgcgcaact tcctcattcc cgcccggacc
      241 cttgcttcca gccagtctga cctgacttcg agcctcgctg tcaccagggc tcctcccttg
      301 gacttaacct cgccccagtt cgaggccatc ccaccttagc taggatgact gtaacccaga
      361 agaacctctt tccctatggg gactatctga actccagcca gttgcacatg gagccagatg
      421 aggttgacac tctgagggaa ggagaggatc cagctgatcg aatgcatccc tatctggcca
      481 tctatgacct tcagcctctg aaagcacacc ccttggtgtt cgcccctggg gtccctgtta
      541 tagcccaggt ggtgggcacc gaaagataca ccagcggatc caaggtggga acctgtactc
      601 tatattctgt tcgcttgacg catggtgact ttacctggac aaccaagaag aagttccgac
      661 actttcagga gctgcatcgg gacctccaga gacacaaagt cttgatgagt ctgctccctt
      721 tggctcgctt tgctgtgacc cattctccag cccgagaggc agccgccgag gatataccct
      781 ccctaccccg aggaggttct gagggctctg ccagacacac agccagcaaa cagaaatact
      841 tggaaaatta cctcaaccgc ctcctgacca tgtctttcta tcgcaattac cacgccatga
      901 cagaatttct ggaagtcagt caactttcct ttatcccaga ccttggctcc aaaggactgg
      961 aaggggtgat ccggaagcgc tcgggcgggc atcgagttcc cggcttcacc ttctgtggcc
     1021 gagaccaagt ttgttatcga tggtccaaga ggtggctggt ggtgaaggac tccttcctgc
     1081 tgtacatgcg cccggagacc ggcgccatct catttgttca gctttttgac cctggctttg
     1141 aggtccaggt cggaaaaagg agcacagaga cgcggtatgg ggtgaggatc gacacctccc
     1201 acaggtccct gattctcaaa tgcagcagct accggcaggc acggtggtgg ggccaggaga
     1261 tcacggagct ggcacagggt tcgggcagag attttctaca gctacatcag catgacagct
     1321 atgccccacc ccggcccggc accctggccc ggtggtttgt gaatggggca ggttactttg
     1381 ctgctgtggc agatgccatc ctgcgagctc aagaggagat tttcatcaca gactggtggt
     1441 tgagtcctga aatttacctg aagcgtccag cccattccga cgactggaga ctggacatta
     1501 tgctcaagag gaaggcggaa gaaggtgtcc gagtttccat actgctgttt aaggaagtgg
     1561 agctggcctt gggcatcaac agtggctaca gcaagaggac gctgatgctg ctgcatccca
     1621 acataaaggt gatgcgacac ccagaccttg tgacactgtg ggctcatcac gagaagctcc
     1681 tggtggtaga ccaagtggtg gcattcttgg gcgggctgga cctggccttc ggccgctggg
     1741 atgacgtgca ataccgactg actgacctgg gtgacccctc tgaacctgta catttacaga
     1801 ctcccacact aggttcagac cctgcagcca ctccagacct ctcgcataac caattcttct
     1861 ggctgggaaa ggactacagc aacctcatca ccaaggactg ggtgcagctg gaccggcctt
     1921 ttgaagattt catcgacagg gagaccacac ccaggatgcc atggagggat gttggagtgg
     1981 ttgtacacgg agtagctgcc agggaccttg cccggcactt catccagcgc tggaatttca
     2041 ccaagaccac caaggccagg tataagacac ctttgtaccc ctacctgctg cccaagtcca
     2101 ccagcactgc aaacaatctc cccttcatga tcccaggcgg gcagtgtgcc actgtgcagg
     2161 tcttgaggtc tgtggatcga tggtcagcag ggacattgga gaactccatc ctcaatgcct
     2221 acctacatac cattcgagag agccagcact ttctctacat tgagaatcag ttcttcatta
     2281 gctgctcaga tgggcgaaca gttctgaaca aggtgggcga tgagattgtg gacagaatcc
     2341 tgaaggctca cgaacagggg cagtgtttcc gagtctactt gcttctgcct ttgctccctg
     2401 gctttgaggg ggacatctcc acagggggtg gtaactccat ccaggctatt ctgcacttca
     2461 cctacaggac cctgtgtcgt ggggaacatt caatcctaca tcgtctcaaa gcagccatgg
     2521 ggactgcgtg gcgagattac atgtccatct gtgggcttcg cacccatgga gagctgggcg
     2581 ggcacccaat ctctgagctc atctatatcc acagcaagat gctcattgcg gatgacagaa
     2641 cagtcatcat tggttctgcg aacatcaatg acaggagctt gctggggaag cgtgacagtg
     2701 agctagccat cctgatcaag gacacagaaa tggaaccatc cctcatggat ggggtggagt
     2761 accaggcagg cagatttgcc ttgagtttgc ggaagcactg tttcagtgtg attcttgggg
     2821 caaatacctg gccagacctg gatctccgag accctgtctg tgatgacttc ttccagctgt
     2881 ggcaagaaac agcggagaac aatgccacca tctatgagca gatcttccgc tgcctgccgt
     2941 ccaatgctac ccgttccctg cgggctctcc gggagtatgt ggctgtggag tccttggcta
     3001 cagtcagccc ttctttggct cagtctgagc ttgcccacat ccagggccac ctagttcact
     3061 tccccctcaa gtttctggag gacgagtcct tgttgccccc actggggagt aaagaaggga
     3121 tgataccttt agaagtgtgg acatagctga ggcttgcgct cagcggcagc cgcgagccgt
     3181 tgggccctat cgtgcctgga tccttgcccc aaaccctgag ttctgagggc agtgcccttc
     3241 gatcctttgg gaggacatct ctgaggactc ctagagaacc acagaggact tgtacttgtg
     3301 aagtagccaa aggaagcact cccaagtctg gcctggggag gcaggagaga gttgtagaga
     3361 ggtttgcctt cctgtcacca agttcaaacc actacgccat agaaccctag ccctacgagg
     3421 cagcctgagg acaagccttt ttattccagg aaaagggact cctgtcttgg gtcttcttca
     3481 tctcactctt cctcctgccc ttgaaccccc tacatgcccc agggcctctc ccagcccact
     3541 gctgcaaaga tggagggagt atggagccac tttgaccact gtctccacca atggtggtgg
     3601 gaaagacacc agaactgcct ccgccagcct gctgacagac actaactttg tactggttca
     3661 tcaagcattt cataaataaa agtgtagaaa cggaaaaaaa aaa
//
