LOCUS       NM_001370985            6562 bp    mRNA    linear   ROD 09-JUN-2024
DEFINITION  Mus musculus phospholipase C, eta 1 (Plch1), transcript variant 5,
            mRNA.
ACCESSION   NM_001370985 XM_017319597
VERSION     NM_001370985.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6562)
  AUTHORS   Thompson,C.L., Ng,L., Menon,V., Martinez,S., Lee,C.K.,
            Glattfelder,K., Sunkin,S.M., Henry,A., Lau,C., Dang,C.,
            Garcia-Lopez,R., Martinez-Ferre,A., Pombero,A., Rubenstein,J.L.R.,
            Wakeman,W.B., Hohmann,J., Dee,N., Sodt,A.J., Young,R., Smith,K.,
            Nguyen,T.N., Kidney,J., Kuan,L., Jeromin,A., Kaykas,A., Miller,J.,
            Page,D., Orta,G., Bernard,A., Riley,Z., Smith,S., Wohnoutka,P.,
            Hawrylycz,M.J., Puelles,L. and Jones,A.R.
  TITLE     A high-resolution spatiotemporal atlas of gene expression of the
            developing mouse brain
  JOURNAL   Neuron 83 (2), 309-323 (2014)
   PUBMED   24952961
REFERENCE   2  (bases 1 to 6562)
  AUTHORS   Zhang,L., Malik,S., Pang,J., Wang,H., Park,K.M., Yule,D.I.,
            Blaxall,B.C. and Smrcka,A.V.
  TITLE     Phospholipase Cepsilon hydrolyzes perinuclear phosphatidylinositol
            4-phosphate to regulate cardiac hypertrophy
  JOURNAL   Cell 153 (1), 216-227 (2013)
   PUBMED   23540699
  REMARK    GeneRIF: Study concludes that perinuclear PLCepsilon, scaffolded to
            mAKAP in cardiac myocytes, responds to hypertrophic stimuli to
            generate diacylglycerol (DAG) from PI4P in the Golgi apparatus, in
            close proximity to the nuclear envelope, to regulate activation of
            nuclear PKD and hypertrophic signaling pathways.
REFERENCE   3  (bases 1 to 6562)
  AUTHORS   Kim,J.K., Choi,J.W., Lim,S., Kwon,O., Seo,J.K., Ryu,S.H. and
            Suh,P.G.
  TITLE     Phospholipase C-eta1 is activated by intracellular Ca(2+)
            mobilization and enhances GPCRs/PLC/Ca(2+) signaling
  JOURNAL   Cell Signal 23 (6), 1022-1029 (2011)
   PUBMED   21262355
  REMARK    GeneRIF: PLC-eta1 is activated via intracellular calcium
            mobilization from the endoplasmic reticulum, and therefore
            amplifies G-protein coupled receptor mediated signaling
REFERENCE   4  (bases 1 to 6562)
  AUTHORS   Dzhura,I., Chepurny,O.G., Kelley,G.G., Leech,C.A., Roe,M.W.,
            Dzhura,E., Afshari,P., Malik,S., Rindler,M.J., Xu,X., Lu,Y.,
            Smrcka,A.V. and Holz,G.G.
  TITLE     Epac2-dependent mobilization of intracellular Ca(2)+ by
            glucagon-like peptide-1 receptor agonist exendin-4 is disrupted in
            beta-cells of phospholipase C-epsilon knockout mice
  JOURNAL   J Physiol 588 (Pt 24), 4871-4889 (2010)
   PUBMED   21041529
  REMARK    GeneRIF: Phospholipase C-eta was found to be expressed in mouse
            beta-cells, and knockout of that gene disrupts the action of
            exendin-4 to facilitate calcium-induced calcium release in the
            beta-cells of these mice.
            Erratum:[J Physiol. 2012 Mar 15;590(Pt 6):1511]
REFERENCE   5  (bases 1 to 6562)
  AUTHORS   Hwang,J.I., Oh,Y.S., Shin,K.J., Kim,H., Ryu,S.H. and Suh,P.G.
  TITLE     Molecular cloning and characterization of a novel phospholipase C,
            PLC-eta
  JOURNAL   Biochem J 389 (Pt 1), 181-186 (2005)
   PUBMED   15702972
  REMARK    GeneRIF: The identification of a novel PLC isoform in the brains of
            human and mouse, named PLC-eta, which contains the conserved
            pleckstrin homology domain, X and Y domains for catalytic activity
            and the C2 domain.
REFERENCE   6  (bases 1 to 6562)
  AUTHORS   Stewart,A.J., Mukherjee,J., Roberts,S.J., Lester,D. and
            Farquharson,C.
  TITLE     Identification of a novel class of mammalian
            phosphoinositol-specific phospholipase C enzymes
  JOURNAL   Int J Mol Med 15 (1), 117-121 (2005)
   PUBMED   15583837
  REMARK    GeneRIF: molecular cloning of PLC-eta1 and PLC-eta2.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC116732.10 and AC121285.7.
            
            On Jun 20, 2019 this sequence version replaced XM_017319597.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMN00849374, SAMN00849375
                              [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-337               AC116732.10        69991-70327         c
            338-484             AC121285.7         15698-15844
            485-728             AC121285.7         18280-18523
            729-858             AC121285.7         26065-26194
            859-1029            AC121285.7         32208-32378
            1030-1123           AC121285.7         44490-44583
            1124-1327           AC121285.7         46504-46707
            1328-1448           AC121285.7         55236-55356
            1449-1620           AC121285.7         59041-59212
            1621-1728           AC121285.7         68111-68218
            1729-1890           AC121285.7         76924-77085
            1891-1962           AC121285.7         78591-78662
            1963-2054           AC121285.7         80556-80647
            2055-2152           AC121285.7         83754-83851
            2153-2332           AC121285.7         88488-88667
            2333-2440           AC121285.7         88764-88871
            2441-2565           AC121285.7         90190-90314
            2566-2650           AC121285.7         92034-92118
            2651-2797           AC121285.7         95172-95318
            2798-2877           AC121285.7         95622-95701
            2878-2937           AC121285.7         97212-97271
            2938-3292           AC121285.7         97697-98051
            3293-3315           AC121285.7         100033-100055
            3316-6562           AC121285.7         100355-103601
FEATURES             Location/Qualifiers
     source          1..6562
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 30.08 cM"
     gene            1..6562
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /note="phospholipase C, eta 1"
                     /db_xref="GeneID:269437"
                     /db_xref="MGI:MGI:2683547"
     exon            1..337
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     CDS             295..3513
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /EC_number="3.1.4.11"
                     /note="isoform 5 is encoded by transcript variant 5;
                     phospholipase C-like protein 3; phosphoinositide
                     phospholipase C-eta-1; 1-phosphatidylinositol
                     4,5-bisphosphate phosphodiesterase eta-1"
                     /codon_start=1
                     /product="1-phosphatidylinositol 4,5-bisphosphate
                     phosphodiesterase eta-1 isoform 5"
                     /protein_id="NP_001357914.1"
                     /db_xref="GeneID:269437"
                     /db_xref="MGI:MGI:2683547"
                     /translation="MADLEVYKNLSPEKVERCMSVMQSGTQMIKLKRGTKGLVRLFYL
                     DEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNH
                     MESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDG
                     LLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLY
                     LLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVSEENKVKNVLGIE
                     GFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYAR
                     VLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSI
                     ENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGKILVKGKKLPYHL
                     GDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKE
                     DPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGKHKKATKSRSKSY
                     STDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSVAAQDIVDDGTTGN
                     VLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVAL
                     NYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLPANPKKQLILKVIS
                     GQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFT
                     VHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITIN
                     EIFGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIG
                     DRILRRTASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMP
                     VDKSLLGALSLPISEAAKDTDGKENCLDLNRKQRKQETRMTEEREPQLEKTHIFQIST
                     KSYPPPPVRSSTKMPTKGQLPVYQTQNSVEDEVQRVRGSNQI"
     exon            338..484
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            485..728
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            729..858
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            859..1029
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            1030..1123
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            1124..1327
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            1328..1448
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            1449..1620
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            1621..1728
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            1729..1890
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            1891..1962
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            1963..2054
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            2055..2152
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            2153..2332
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            2333..2440
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            2441..2565
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            2566..2650
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            2651..2797
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            2798..2877
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            2878..2937
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            2938..3292
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            3293..3315
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            3316..6562
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6539..6544
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /note="hexamer: ATTAAA"
     polyA_site      6562
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /note="major polyA site"
ORIGIN      
        1 gcattcccac agtagcggca ggcagagcag ttgcttcgag tgtgcttatc tggctgcact
       61 gttcacttta ggacaaagtt ctacagtcat ctcatggatt tcttttctct acattttaaa
      121 acctgggctg tgacgatgtc atcatgccat cagaggcagc atgagccaag tgcttggggc
      181 tgctgaggat ttttttgcct tctccccttt tggagatgtg tatcagaaaa aggctttgcc
      241 tttagagaaa cattttattg ctttggccaa taatgtgtag caatattaaa tttaatggca
      301 gaccttgaag tgtataaaaa cttaagtcca gaaaaagttg aaagatgcat gagtgtaatg
      361 cagtccggga cacagatgat caaactgaag cgtggcacca aagggctcgt tcgcctcttt
      421 tacctggatg agcaccggac ccgcctccga tggcgaccct ctcggaagag tgagaaggca
      481 aaaatactta ttgactccat ctacaaagtc accgagggca ggcagtctga aatattccac
      541 agacaggctg aggggaactt tgaccctagc tgctgcttta ccatctacca tggcaaccac
      601 atggagtctc tggacctcat tacctccaac ccagaggagg cacgcacctg gatcacgggc
      661 ctcaagtatc tgatggctgg catcagtgat gaagactccc ttgccaagag gcagaggacc
      721 catgaccaat gggtgaagca gacctttgag gaagctgata agaacggtga tggcttattg
      781 aatattgaag agattcacca gctgatgcat aaactaaatg tcaatctgcc ccgcagaaaa
      841 gtcaggcaaa tgtttcagga agcagataca gatgagaatc agggaacctt gacatttgaa
      901 gagttctgcg ttttttacaa aatgatgtca ttgagacgag acctctattt gctgctcttg
      961 agctacagtg acaagaaaga ccacctgact gtggaggagc tggctcagtt tttgaaagtg
     1021 gaacaaaaga tgagtaatgt gacactggac tattgtcttg acatcataat gaagtttgaa
     1081 gtttctgaag aaaacaaagt gaaaaacgtc cttggtatag aaggcttcac gaacttcatg
     1141 cgtagccctg cctgtgacgt atttaacccg ttgcaccatg aagtgtacca agacatggat
     1201 cagcccctgt gcaactacta cattgcttca tctcacaaca catacctgac tggggatcag
     1261 ctcctttctc aatctaaagt ggatatgtat gcacgggtgc tacaagaggg ttgtagatgc
     1321 gtggaagttg actgttggga tggcccagat ggagagccag tggtccacca tggttatact
     1381 ctcacttcaa aaattctctt cagagatgtt gtggagacca tcaacaagca tgcattcgtg
     1441 aagaatgagt tcccggttat cctgtccatt gagaaccact gcagcattca gcagcagagg
     1501 aagattgctc agtacctgaa aggtatattg caggacaaac tggacctgtc ttctgtagac
     1561 accggagagt gcaggcagct tccaagccct cagagtctga aaggcaaaat cctagtgaag
     1621 ggcaagaagt tgccgtatca ccttggggat gatgcagagg aaggagaagt gtccgatgag
     1681 gacagtgcag atgagattga agatgagtgc aagttcaagc tgcattacag taacgggacc
     1741 actgagcacc aggtagaatc tttcatacgg aaaaagctgg agtcactgtt gaaggagtct
     1801 cagattcgag acaaagaaga tccagacagt ttcacagtga gggcgttact caaggctaca
     1861 catgaaggct taaatgcaca cctgaagcag aacctggatg taaaggaaag tggaaagaag
     1921 tcccatgggc gatccctgat ggccaacttt gggaaacaca agaaagctac gaaatcacgt
     1981 tctaaatcct atagtactga tgatgaggat gatagccttc agaatcctgg caaggaggga
     2041 ggccagctgt acaggctggg ccgccgaagg agaaccatga aactctgcag agagctctcg
     2101 gacttggtgg tgtacacaaa ctccgtggca gcccaggata ttgttgatga tggaaccaca
     2161 gggaatgtgt tgtccttcag cgaaacaaga gcccatcaag tggttcagca gaagtcagag
     2221 cagttcatga tctacaacca aaaacagctc acaaggattt acccctctgc ctaccgcatc
     2281 gattccagta acttcaaccc actgccctac tggaatgcag gctgccagct cgtggctctg
     2341 aactaccagt ctgaagggcg aatgatgcag atcaatcgag caaaattcaa ggcgaatggc
     2401 aactgtggct atattctcaa gccccagcaa atgtgcaaag gtactttcaa ccctttctct
     2461 ggtgacccac ttcctgccaa ccccaaaaaa cagctcatac tgaaagtgat cagtggacag
     2521 caactcccca aacctcctga ctctatgttt ggagaccgag gcgagatcat tgaccctttc
     2581 gttgaagttg aaattattgg gctgccagta gactgttgca aggaccaaac tcgtgtggtg
     2641 gatgacaacg gatttaaccc tgtgtgggaa gaaaccctaa cttttacagt tcacatgcca
     2701 gaaatagctt tggttcggtt ccttgtgtgg gatcatgatc ctattggaag agactttgtc
     2761 ggacaaagga ctgtgacctt cagtagcctc gtgcccggct accggcatgt ctacctggaa
     2821 ggactcacag aagcatctat tttcgttcac ataaccatca atgagatctt tggaaagtgg
     2881 agtcctttaa tcctcaaccc cagttatacc atattgcact ttctaggagc tacaaagaac
     2941 agacagctcc aaggcctgaa ggggctgttc aataagaatc ccaggcacgc ttcttcagaa
     3001 aacaactcgc attatgttcg gaagcgatcg attggagata ggattctgcg acgtacagct
     3061 agtgctccag ccaaaggcag gaaaaagagc aaagtgggct tccaagaaat ggttgagata
     3121 aaagattctg tctctgaggc ttcaagagat caagatggtg tcctgaggag gaccacccgg
     3181 agtctgcaag tacgccctgt ctctatgcct gttgacaaga gccttctggg ggctttgtcg
     3241 ctgcccatat ctgaagcagc aaaagacacg gatggaaaag aaaactgtct ggatctgaat
     3301 aggaagcagc gcaagcagga gacaaggatg acagaagaaa gggagccgca actagaaaag
     3361 acccacattt ttcaaatttc aacaaaaagt tatcctcctc ctccagtgcg ctcctccaca
     3421 aagatgccaa ccaagggcca actgccagtg tatcaaaccc agaacagtgt ggaggacgag
     3481 gtgcaaagag tgagaggatc aaaccaaata tgacaaatga ttgccaggaa aaccacaatc
     3541 ccccgaaatt cctttctcca aggaagcatt tggctctgga tcctgcaacg aagggactgc
     3601 aagagagact gcatggaatg aaaaccaatg agaaggagca tgccgagggc ttcttgggag
     3661 agaaaagcat gctgtccgga agcgttcttt ctcaaagttc tctggaggta gagaacctgg
     3721 aaggcagcag agccaagggc agagctgcaa cctccttttc tctctcggac gtctccgcgc
     3781 tctgttctga catccctgat ttacattcaa ctgccattct acaggacact gaaatttcca
     3841 atctcattga tgacgtgact ttaacaaatg agaatcagtc gggaagttcc atctcggcac
     3901 tgattggcca gtttgaagaa agcaaccacc cggctaacgt tactgtggtt tctcatctca
     3961 gcaccagtgg ggcatcagga agtgctcctt tccaaacacc cttcaagcat ggtctttccc
     4021 aggggaacca gaaggcctcc ttcttgtgct catctcctga gctgaataag ctctctagtg
     4081 ttgagaccac caaactggca aataacgcag ttccttgtgg agtgattggt tctcccatct
     4141 ctacaccaaa gccaggtgat gacccttcag ataaggccaa gacaagagtc attgaaggca
     4201 acctgcctgg gttccctgat gcttctcctg gtcaatttcc caaaagcccc acccatggag
     4261 aggaccatag tcaagtgatg aacagccctg ctctctccac ggagttggcc atagaagata
     4321 taattgcaga ccctgctctc tctataaatt ctgcagaaag cagtcttgtg gaaattgatg
     4381 gagaatctga aaacctatct ctaacaacct gtgactacag ggaagaagct ccaagtcaac
     4441 ttgtttctcc actaaaactg cagcagagcc aggagatggt ggaacacatt cagaggggct
     4501 tgaggaatgg ctactgtaaa gagactctcc tcccttctga aatattcaac aatattccag
     4561 gtgtcaaaaa tcacagtatt tctcatctaa cctatcaggg tgctggcttt gtgtataacc
     4621 atttctcaag ttcagatgca aaaacgaacc aaatctgtga gccccagcag cctagggctc
     4681 cagatatgca tgcccctaca cctacaccat caacacatgc tcctttggct gctttgaaac
     4741 tgccaagccc atgcaaatcc aaaagtctgg gggacttaac atcagaggac attgcctgca
     4801 attttgaaag caaatatcaa tgtattagta ggagctttgt gacaaacggc attagggaca
     4861 agagcgtgac tatgaagaca aagtcattag agcctttaga cgccctgact gaacagctcc
     4921 ggaagctggt gtcctttgac caagaagaca gctgtcaagt gctctactca aagcaggatg
     4981 tcaatcagtg tcccagggca ttagtcagaa agttgtcgtc tagaagtcag agcagagtgc
     5041 gcaacattgc tagccgtgcc aaggagaagc aggaagctgg caagcaaaaa gctatggccc
     5101 agagcaccag aggaggagtg gtccttagaa gtaaaccgcc cgctcctgct ctggccgtga
     5161 accgtcactc cacgggctca tacattgcaa gctacctgag gaacatgaaa gctggtggcc
     5221 tagagggtcg aggcatccca gagggagcgt gcacggccct tcgctatggc tacatggacc
     5281 agttttgttc agataattca gttctgcaga ctgagccaag cagcgaagat aaacccgaaa
     5341 tttattttct tttgaggctt tgaattatat aaagttgcat tttcattgtt tacatgatac
     5401 tctagaagat gttagatttt tttccattgt caggaaatat gatactgggc tttgaaatga
     5461 tttccaaatg tatttttaaa cttgtatttt ggtctccctt tgcctcatgc atttgtatat
     5521 agattctgtg gcctttccca ggaatccatg atctttcccc caaggtgttg taaactatgt
     5581 tagtatgaaa tgtgtgcatg cccttatgtg aaacttctca gcagcttggg tcatagagtg
     5641 catatgttgc acacaaaaaa atgtgtttcc ccatgtcctc cgatcacagt cccagaggct
     5701 gtcaccgtta ctcactaagt gttgagctca ttaagattaa actttgaaaa ccataaaggg
     5761 atggattctt tatgtgaagt tacattttcc ttctgaattg agagactcag attcagtttt
     5821 ataatccatt taaaagagat gttttaagtg gttacattgc cctttatgat ttccacatgc
     5881 cacccaaagt aggaattcca tgaagatgga tagcagctca ccaaccccaa gtccctttta
     5941 gaatggacta tgaggaagaa agcgaacttc tgtatataag ggcaaattag tttgttttgc
     6001 atgaaatttt gttacatatt tttgctaatg gccttgtatg taacaagaat ccagttgcca
     6061 agctatctgt cattcttttg gatgatgtaa tattatagat tgaaaataat ggctttccga
     6121 atgagagacg ttcagctggg gatggtggtt cccgacttta atcccagtag aagcagatga
     6181 gtttgaggcc agcctgatct acatagtaag ctccaggcca atcaaagcta tgtactcagt
     6241 ccctgtctta aaacagcaac aacgaaaaga tagtgagaga cttacattaa acttaaaacc
     6301 caaagtagga caaacagaca cttccccaaa gcaatcaaga aaaaaagtat tttctcataa
     6361 tccaagtgaa tagataatta aaagaagcct tttcccttta gggaaccaag taactctatt
     6421 ttagtaccga catatattat tttcattgtg gatccatttt ttattgcatg ttcagatgtc
     6481 atttgctttt tgtaccatga attacaatga tgttcttcct ggacctcata aaaatataat
     6541 taaagcagat ttttgaacac tt
//
