LOCUS       NM_001370984            6586 bp    mRNA    linear   ROD 09-JUN-2024
DEFINITION  Mus musculus phospholipase C, eta 1 (Plch1), transcript variant 4,
            mRNA.
ACCESSION   NM_001370984 XM_006501537
VERSION     NM_001370984.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6586)
  AUTHORS   Thompson,C.L., Ng,L., Menon,V., Martinez,S., Lee,C.K.,
            Glattfelder,K., Sunkin,S.M., Henry,A., Lau,C., Dang,C.,
            Garcia-Lopez,R., Martinez-Ferre,A., Pombero,A., Rubenstein,J.L.R.,
            Wakeman,W.B., Hohmann,J., Dee,N., Sodt,A.J., Young,R., Smith,K.,
            Nguyen,T.N., Kidney,J., Kuan,L., Jeromin,A., Kaykas,A., Miller,J.,
            Page,D., Orta,G., Bernard,A., Riley,Z., Smith,S., Wohnoutka,P.,
            Hawrylycz,M.J., Puelles,L. and Jones,A.R.
  TITLE     A high-resolution spatiotemporal atlas of gene expression of the
            developing mouse brain
  JOURNAL   Neuron 83 (2), 309-323 (2014)
   PUBMED   24952961
REFERENCE   2  (bases 1 to 6586)
  AUTHORS   Zhang,L., Malik,S., Pang,J., Wang,H., Park,K.M., Yule,D.I.,
            Blaxall,B.C. and Smrcka,A.V.
  TITLE     Phospholipase Cepsilon hydrolyzes perinuclear phosphatidylinositol
            4-phosphate to regulate cardiac hypertrophy
  JOURNAL   Cell 153 (1), 216-227 (2013)
   PUBMED   23540699
  REMARK    GeneRIF: Study concludes that perinuclear PLCepsilon, scaffolded to
            mAKAP in cardiac myocytes, responds to hypertrophic stimuli to
            generate diacylglycerol (DAG) from PI4P in the Golgi apparatus, in
            close proximity to the nuclear envelope, to regulate activation of
            nuclear PKD and hypertrophic signaling pathways.
REFERENCE   3  (bases 1 to 6586)
  AUTHORS   Kim,J.K., Choi,J.W., Lim,S., Kwon,O., Seo,J.K., Ryu,S.H. and
            Suh,P.G.
  TITLE     Phospholipase C-eta1 is activated by intracellular Ca(2+)
            mobilization and enhances GPCRs/PLC/Ca(2+) signaling
  JOURNAL   Cell Signal 23 (6), 1022-1029 (2011)
   PUBMED   21262355
  REMARK    GeneRIF: PLC-eta1 is activated via intracellular calcium
            mobilization from the endoplasmic reticulum, and therefore
            amplifies G-protein coupled receptor mediated signaling
REFERENCE   4  (bases 1 to 6586)
  AUTHORS   Dzhura,I., Chepurny,O.G., Kelley,G.G., Leech,C.A., Roe,M.W.,
            Dzhura,E., Afshari,P., Malik,S., Rindler,M.J., Xu,X., Lu,Y.,
            Smrcka,A.V. and Holz,G.G.
  TITLE     Epac2-dependent mobilization of intracellular Ca(2)+ by
            glucagon-like peptide-1 receptor agonist exendin-4 is disrupted in
            beta-cells of phospholipase C-epsilon knockout mice
  JOURNAL   J Physiol 588 (Pt 24), 4871-4889 (2010)
   PUBMED   21041529
  REMARK    GeneRIF: Phospholipase C-eta was found to be expressed in mouse
            beta-cells, and knockout of that gene disrupts the action of
            exendin-4 to facilitate calcium-induced calcium release in the
            beta-cells of these mice.
            Erratum:[J Physiol. 2012 Mar 15;590(Pt 6):1511]
REFERENCE   5  (bases 1 to 6586)
  AUTHORS   Hwang,J.I., Oh,Y.S., Shin,K.J., Kim,H., Ryu,S.H. and Suh,P.G.
  TITLE     Molecular cloning and characterization of a novel phospholipase C,
            PLC-eta
  JOURNAL   Biochem J 389 (Pt 1), 181-186 (2005)
   PUBMED   15702972
  REMARK    GeneRIF: The identification of a novel PLC isoform in the brains of
            human and mouse, named PLC-eta, which contains the conserved
            pleckstrin homology domain, X and Y domains for catalytic activity
            and the C2 domain.
REFERENCE   6  (bases 1 to 6586)
  AUTHORS   Stewart,A.J., Mukherjee,J., Roberts,S.J., Lester,D. and
            Farquharson,C.
  TITLE     Identification of a novel class of mammalian
            phosphoinositol-specific phospholipase C enzymes
  JOURNAL   Int J Mol Med 15 (1), 117-121 (2005)
   PUBMED   15583837
  REMARK    GeneRIF: molecular cloning of PLC-eta1 and PLC-eta2.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC116732.10 and AC121285.7.
            
            On Jun 20, 2019 this sequence version replaced XM_006501537.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMN00849374, SAMN00849375
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-236               AC116732.10        118269-118504       c
            237-322             AC116732.10        104970-105055       c
            323-441             AC116732.10        89698-89816         c
            442-588             AC121285.7         15698-15844
            589-832             AC121285.7         18280-18523
            833-962             AC121285.7         26065-26194
            963-1133            AC121285.7         32208-32378
            1134-1227           AC121285.7         44490-44583
            1228-1431           AC121285.7         46504-46707
            1432-1552           AC121285.7         55236-55356
            1553-1724           AC121285.7         59041-59212
            1725-1832           AC121285.7         68111-68218
            1833-1994           AC121285.7         76924-77085
            1995-2066           AC121285.7         78591-78662
            2067-2161           AC121285.7         80553-80647
            2162-2259           AC121285.7         83754-83851
            2260-2439           AC121285.7         88488-88667
            2440-2547           AC121285.7         88764-88871
            2548-2672           AC121285.7         90190-90314
            2673-2757           AC121285.7         92034-92118
            2758-2904           AC121285.7         95172-95318
            2905-2984           AC121285.7         95622-95701
            2985-3339           AC121285.7         97697-98051
            3340-6586           AC121285.7         100355-103601
FEATURES             Location/Qualifiers
     source          1..6586
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 30.08 cM"
     gene            1..6586
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /note="phospholipase C, eta 1"
                     /db_xref="GeneID:269437"
                     /db_xref="MGI:MGI:2683547"
     exon            1..236
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            237..322
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            323..441
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     CDS             363..5387
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /EC_number="3.1.4.11"
                     /note="isoform 4 is encoded by transcript variant 4;
                     phospholipase C-like protein 3; phosphoinositide
                     phospholipase C-eta-1; 1-phosphatidylinositol
                     4,5-bisphosphate phosphodiesterase eta-1"
                     /codon_start=1
                     /product="1-phosphatidylinositol 4,5-bisphosphate
                     phosphodiesterase eta-1 isoform 4"
                     /protein_id="NP_001357913.1"
                     /db_xref="GeneID:269437"
                     /db_xref="MGI:MGI:2683547"
                     /translation="MNYWNGEKRNCVQYRRHFLVDNRVFHVERCMSVMQSGTQMIKLK
                     RGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFD
                     PSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVK
                     QTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCV
                     FYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMSNVTLDYCLDIIMKFEVS
                     EENKVKNVLGIEGFTNFMRSPACDVFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQ
                     LLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHA
                     FVKNEFPVILSIENHCSIQQQRKIAQYLKGILQDKLDLSSVDTGECRQLPSPQSLKGK
                     ILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLE
                     SLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQNLDVKESGKKSHGRSLMANFGK
                     HKQKATKSRSKSYSTDDEDDSLQNPGKEGGQLYRLGRRRRTMKLCRELSDLVVYTNSV
                     AAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNP
                     LPYWNAGCQLVALNYQSEGRMMQINRAKFKANGNCGYILKPQQMCKGTFNPFSGDPLP
                     ANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDN
                     GFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEG
                     LTEASIFVHITINEIFGKNRQLQGLKGLFNKNPRHASSENNSHYVRKRSIGDRILRRT
                     ASAPAKGRKKSKVGFQEMVEIKDSVSEASRDQDGVLRRTTRSLQVRPVSMPVDKSLLG
                     ALSLPISEAAKDTDGKENCLAGDKDDRRKGAATRKDPHFSNFNKKLSSSSSALLHKDA
                     NQGPTASVSNPEQCGGRGAKSERIKPNMTNDCQENHNPPKFLSPRKHLALDPATKGLQ
                     ERLHGMKTNEKEHAEGFLGEKSMLSGSVLSQSSLEVENLEGSRAKGRAATSFSLSDVS
                     ALCSDIPDLHSTAILQDTEISNLIDDVTLTNENQSGSSISALIGQFEESNHPANVTVV
                     SHLSTSGASGSAPFQTPFKHGLSQGNQKASFLCSSPELNKLSSVETTKLANNAVPCGV
                     IGSPISTPKPGDDPSDKAKTRVIEGNLPGFPDASPGQFPKSPTHGEDHSQVMNSPALS
                     TELAIEDIIADPALSINSAESSLVEIDGESENLSLTTCDYREEAPSQLVSPLKLQQSQ
                     EMVEHIQRGLRNGYCKETLLPSEIFNNIPGVKNHSISHLTYQGAGFVYNHFSSSDAKT
                     NQICEPQQPRAPDMHAPTPTPSTHAPLAALKLPSPCKSKSLGDLTSEDIACNFESKYQ
                     CISRSFVTNGIRDKSVTMKTKSLEPLDALTEQLRKLVSFDQEDSCQVLYSKQDVNQCP
                     RALVRKLSSRSQSRVRNIASRAKEKQEAGKQKAMAQSTRGGVVLRSKPPAPALAVNRH
                     STGSYIASYLRNMKAGGLEGRGIPEGACTALRYGYMDQFCSDNSVLQTEPSSEDKPEI
                     YFLLRL"
     exon            442..588
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            589..832
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            833..962
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            963..1133
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            1134..1227
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            1228..1431
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            1432..1552
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            1553..1724
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            1725..1832
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            1833..1994
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            1995..2066
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            2067..2161
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            2162..2259
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            2260..2439
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            2440..2547
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            2548..2672
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            2673..2757
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            2758..2904
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            2905..2984
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            2985..3339
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     exon            3340..6586
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6563..6568
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /note="hexamer: ATTAAA"
     polyA_site      6586
                     /gene="Plch1"
                     /gene_synonym="PLC-eta-1; PLCeta1; Plcl3"
                     /note="major polyA site"
ORIGIN      
        1 aggatttgaa tgagaaagca ggttgtggcg gcagcgctgg tctttacttg gagctcctct
       61 gttggttctg ttggctggtg tgtagtggtg cccatcttgg gctggggggc tgttttggag
      121 gaattggggt tggagtcaca ccgggacact agcagatcac tccagcattt gggaggttga
      181 gccggttaca aaaactccga ggatccttca tcagcgggat actcagagct gagcagcaag
      241 gcattccatt gctggagctg aattcctcac ttcaggctgg aagaaaataa tgcacgtaac
      301 aaagagaatc taactctccc agggcaattt catttggatg ctgtgccaga catcttccaa
      361 caatgaacta ctggaacggt gagaagagga actgtgtgca gtaccgcaga cattttctgg
      421 tggacaaccg cgtgtttcat gttgaaagat gcatgagtgt aatgcagtcc gggacacaga
      481 tgatcaaact gaagcgtggc accaaagggc tcgttcgcct cttttacctg gatgagcacc
      541 ggacccgcct ccgatggcga ccctctcgga agagtgagaa ggcaaaaata cttattgact
      601 ccatctacaa agtcaccgag ggcaggcagt ctgaaatatt ccacagacag gctgagggga
      661 actttgaccc tagctgctgc tttaccatct accatggcaa ccacatggag tctctggacc
      721 tcattacctc caacccagag gaggcacgca cctggatcac gggcctcaag tatctgatgg
      781 ctggcatcag tgatgaagac tcccttgcca agaggcagag gacccatgac caatgggtga
      841 agcagacctt tgaggaagct gataagaacg gtgatggctt attgaatatt gaagagattc
      901 accagctgat gcataaacta aatgtcaatc tgccccgcag aaaagtcagg caaatgtttc
      961 aggaagcaga tacagatgag aatcagggaa ccttgacatt tgaagagttc tgcgtttttt
     1021 acaaaatgat gtcattgaga cgagacctct atttgctgct cttgagctac agtgacaaga
     1081 aagaccacct gactgtggag gagctggctc agtttttgaa agtggaacaa aagatgagta
     1141 atgtgacact ggactattgt cttgacatca taatgaagtt tgaagtttct gaagaaaaca
     1201 aagtgaaaaa cgtccttggt atagaaggct tcacgaactt catgcgtagc cctgcctgtg
     1261 acgtatttaa cccgttgcac catgaagtgt accaagacat ggatcagccc ctgtgcaact
     1321 actacattgc ttcatctcac aacacatacc tgactgggga tcagctcctt tctcaatcta
     1381 aagtggatat gtatgcacgg gtgctacaag agggttgtag atgcgtggaa gttgactgtt
     1441 gggatggccc agatggagag ccagtggtcc accatggtta tactctcact tcaaaaattc
     1501 tcttcagaga tgttgtggag accatcaaca agcatgcatt cgtgaagaat gagttcccgg
     1561 ttatcctgtc cattgagaac cactgcagca ttcagcagca gaggaagatt gctcagtacc
     1621 tgaaaggtat attgcaggac aaactggacc tgtcttctgt agacaccgga gagtgcaggc
     1681 agcttccaag ccctcagagt ctgaaaggca aaatcctagt gaagggcaag aagttgccgt
     1741 atcaccttgg ggatgatgca gaggaaggag aagtgtccga tgaggacagt gcagatgaga
     1801 ttgaagatga gtgcaagttc aagctgcatt acagtaacgg gaccactgag caccaggtag
     1861 aatctttcat acggaaaaag ctggagtcac tgttgaagga gtctcagatt cgagacaaag
     1921 aagatccaga cagtttcaca gtgagggcgt tactcaaggc tacacatgaa ggcttaaatg
     1981 cacacctgaa gcagaacctg gatgtaaagg aaagtggaaa gaagtcccat gggcgatccc
     2041 tgatggccaa ctttgggaaa cacaagcaga aagctacgaa atcacgttct aaatcctata
     2101 gtactgatga tgaggatgat agccttcaga atcctggcaa ggagggaggc cagctgtaca
     2161 ggctgggccg ccgaaggaga accatgaaac tctgcagaga gctctcggac ttggtggtgt
     2221 acacaaactc cgtggcagcc caggatattg ttgatgatgg aaccacaggg aatgtgttgt
     2281 ccttcagcga aacaagagcc catcaagtgg ttcagcagaa gtcagagcag ttcatgatct
     2341 acaaccaaaa acagctcaca aggatttacc cctctgccta ccgcatcgat tccagtaact
     2401 tcaacccact gccctactgg aatgcaggct gccagctcgt ggctctgaac taccagtctg
     2461 aagggcgaat gatgcagatc aatcgagcaa aattcaaggc gaatggcaac tgtggctata
     2521 ttctcaagcc ccagcaaatg tgcaaaggta ctttcaaccc tttctctggt gacccacttc
     2581 ctgccaaccc caaaaaacag ctcatactga aagtgatcag tggacagcaa ctccccaaac
     2641 ctcctgactc tatgtttgga gaccgaggcg agatcattga ccctttcgtt gaagttgaaa
     2701 ttattgggct gccagtagac tgttgcaagg accaaactcg tgtggtggat gacaacggat
     2761 ttaaccctgt gtgggaagaa accctaactt ttacagttca catgccagaa atagctttgg
     2821 ttcggttcct tgtgtgggat catgatccta ttggaagaga ctttgtcgga caaaggactg
     2881 tgaccttcag tagcctcgtg cccggctacc ggcatgtcta cctggaagga ctcacagaag
     2941 catctatttt cgttcacata accatcaatg agatctttgg aaagaacaga cagctccaag
     3001 gcctgaaggg gctgttcaat aagaatccca ggcacgcttc ttcagaaaac aactcgcatt
     3061 atgttcggaa gcgatcgatt ggagatagga ttctgcgacg tacagctagt gctccagcca
     3121 aaggcaggaa aaagagcaaa gtgggcttcc aagaaatggt tgagataaaa gattctgtct
     3181 ctgaggcttc aagagatcaa gatggtgtcc tgaggaggac cacccggagt ctgcaagtac
     3241 gccctgtctc tatgcctgtt gacaagagcc ttctgggggc tttgtcgctg cccatatctg
     3301 aagcagcaaa agacacggat ggaaaagaaa actgtctggc aggagacaag gatgacagaa
     3361 gaaagggagc cgcaactaga aaagacccac atttttcaaa tttcaacaaa aagttatcct
     3421 cctcctccag tgcgctcctc cacaaagatg ccaaccaagg gccaactgcc agtgtatcaa
     3481 acccagaaca gtgtggagga cgaggtgcaa agagtgagag gatcaaacca aatatgacaa
     3541 atgattgcca ggaaaaccac aatcccccga aattcctttc tccaaggaag catttggctc
     3601 tggatcctgc aacgaaggga ctgcaagaga gactgcatgg aatgaaaacc aatgagaagg
     3661 agcatgccga gggcttcttg ggagagaaaa gcatgctgtc cggaagcgtt ctttctcaaa
     3721 gttctctgga ggtagagaac ctggaaggca gcagagccaa gggcagagct gcaacctcct
     3781 tttctctctc ggacgtctcc gcgctctgtt ctgacatccc tgatttacat tcaactgcca
     3841 ttctacagga cactgaaatt tccaatctca ttgatgacgt gactttaaca aatgagaatc
     3901 agtcgggaag ttccatctcg gcactgattg gccagtttga agaaagcaac cacccggcta
     3961 acgttactgt ggtttctcat ctcagcacca gtggggcatc aggaagtgct cctttccaaa
     4021 cacccttcaa gcatggtctt tcccagggga accagaaggc ctccttcttg tgctcatctc
     4081 ctgagctgaa taagctctct agtgttgaga ccaccaaact ggcaaataac gcagttcctt
     4141 gtggagtgat tggttctccc atctctacac caaagccagg tgatgaccct tcagataagg
     4201 ccaagacaag agtcattgaa ggcaacctgc ctgggttccc tgatgcttct cctggtcaat
     4261 ttcccaaaag ccccacccat ggagaggacc atagtcaagt gatgaacagc cctgctctct
     4321 ccacggagtt ggccatagaa gatataattg cagaccctgc tctctctata aattctgcag
     4381 aaagcagtct tgtggaaatt gatggagaat ctgaaaacct atctctaaca acctgtgact
     4441 acagggaaga agctccaagt caacttgttt ctccactaaa actgcagcag agccaggaga
     4501 tggtggaaca cattcagagg ggcttgagga atggctactg taaagagact ctcctccctt
     4561 ctgaaatatt caacaatatt ccaggtgtca aaaatcacag tatttctcat ctaacctatc
     4621 agggtgctgg ctttgtgtat aaccatttct caagttcaga tgcaaaaacg aaccaaatct
     4681 gtgagcccca gcagcctagg gctccagata tgcatgcccc tacacctaca ccatcaacac
     4741 atgctccttt ggctgctttg aaactgccaa gcccatgcaa atccaaaagt ctgggggact
     4801 taacatcaga ggacattgcc tgcaattttg aaagcaaata tcaatgtatt agtaggagct
     4861 ttgtgacaaa cggcattagg gacaagagcg tgactatgaa gacaaagtca ttagagcctt
     4921 tagacgccct gactgaacag ctccggaagc tggtgtcctt tgaccaagaa gacagctgtc
     4981 aagtgctcta ctcaaagcag gatgtcaatc agtgtcccag ggcattagtc agaaagttgt
     5041 cgtctagaag tcagagcaga gtgcgcaaca ttgctagccg tgccaaggag aagcaggaag
     5101 ctggcaagca aaaagctatg gcccagagca ccagaggagg agtggtcctt agaagtaaac
     5161 cgcccgctcc tgctctggcc gtgaaccgtc actccacggg ctcatacatt gcaagctacc
     5221 tgaggaacat gaaagctggt ggcctagagg gtcgaggcat cccagaggga gcgtgcacgg
     5281 cccttcgcta tggctacatg gaccagtttt gttcagataa ttcagttctg cagactgagc
     5341 caagcagcga agataaaccc gaaatttatt ttcttttgag gctttgaatt atataaagtt
     5401 gcattttcat tgtttacatg atactctaga agatgttaga tttttttcca ttgtcaggaa
     5461 atatgatact gggctttgaa atgatttcca aatgtatttt taaacttgta ttttggtctc
     5521 cctttgcctc atgcatttgt atatagattc tgtggccttt cccaggaatc catgatcttt
     5581 cccccaaggt gttgtaaact atgttagtat gaaatgtgtg catgccctta tgtgaaactt
     5641 ctcagcagct tgggtcatag agtgcatatg ttgcacacaa aaaaatgtgt ttccccatgt
     5701 cctccgatca cagtcccaga ggctgtcacc gttactcact aagtgttgag ctcattaaga
     5761 ttaaactttg aaaaccataa agggatggat tctttatgtg aagttacatt ttccttctga
     5821 attgagagac tcagattcag ttttataatc catttaaaag agatgtttta agtggttaca
     5881 ttgcccttta tgatttccac atgccaccca aagtaggaat tccatgaaga tggatagcag
     5941 ctcaccaacc ccaagtccct tttagaatgg actatgagga agaaagcgaa cttctgtata
     6001 taagggcaaa ttagtttgtt ttgcatgaaa ttttgttaca tatttttgct aatggccttg
     6061 tatgtaacaa gaatccagtt gccaagctat ctgtcattct tttggatgat gtaatattat
     6121 agattgaaaa taatggcttt ccgaatgaga gacgttcagc tggggatggt ggttcccgac
     6181 tttaatccca gtagaagcag atgagtttga ggccagcctg atctacatag taagctccag
     6241 gccaatcaaa gctatgtact cagtccctgt cttaaaacag caacaacgaa aagatagtga
     6301 gagacttaca ttaaacttaa aacccaaagt aggacaaaca gacacttccc caaagcaatc
     6361 aagaaaaaaa gtattttctc ataatccaag tgaatagata attaaaagaa gccttttccc
     6421 tttagggaac caagtaactc tattttagta ccgacatata ttattttcat tgtggatcca
     6481 ttttttattg catgttcaga tgtcatttgc tttttgtacc atgaattaca atgatgttct
     6541 tcctggacct cataaaaata taattaaagc agatttttga acactt
//
