LOCUS       NM_019588               9525 bp    mRNA    linear   ROD 06-OCT-2024
DEFINITION  Mus musculus phospholipase C, epsilon 1 (Plce1), mRNA.
ACCESSION   NM_019588
VERSION     NM_019588.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 9525)
  AUTHORS   Gupta,A., Thirunavukkarasu,S., Rangel-Moreno,J., Ahmed,M.,
            Swanson,R.V., Mbandi,S.K., Smrcka,A.V., Kaushal,D., Scriba,T.J. and
            Khader,S.A.
  TITLE     Phospholipase C epsilon-1 (PLCE1) mediates macrophage activation
            and protection against tuberculosis
  JOURNAL   Infect Immun 92 (4), e0049523 (2024)
   PUBMED   38451080
  REMARK    GeneRIF: Phospholipase C epsilon-1 (PLCE1) mediates macrophage
            activation and protection against tuberculosis.
REFERENCE   2  (bases 1 to 9525)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 9525)
  AUTHORS   Chen,X., Coffman,B.L., Brindley,R.L., Galpin,J.D., Ahern,C.A.,
            Currie,K.P.M., Smrcka,A.V., Axelrod,D. and Anantharam,A.
  TITLE     Phospholipase C-epsilon defines a PACAP-stimulated pathway for
            secretion in the chromaffin cell
  JOURNAL   J Neuroendocrinol 35 (11), e13255 (2023)
   PUBMED   36970756
  REMARK    GeneRIF: Phospholipase C-epsilon defines a PACAP-stimulated pathway
            for secretion in the chromaffin cell.
REFERENCE   4  (bases 1 to 9525)
  AUTHORS   Atchison,D.K., O'Connor,C.L., Converso-Baran,K., Bergin,I.L.,
            Zhang,H., Wang,Y., Hartman,J.R., Ju,W., Smrcka,A.V., Ganesh,S.K.
            and Bitzer,M.
  TITLE     Phospholipase Cepsilon insufficiency causes ascending aortic
            aneurysm and dissection
  JOURNAL   Am J Physiol Heart Circ Physiol 323 (6), H1376-H1387 (2022)
   PUBMED   36367690
  REMARK    GeneRIF: Phospholipase Cepsilon insufficiency causes ascending
            aortic aneurysm and dissection.
REFERENCE   5  (bases 1 to 9525)
  AUTHORS   Atchison,D.K., O'Connor,C.L., Menon,R., Otto,E.A., Ganesh,S.K.,
            Wiggins,R.C., Smrcka,A.V. and Bitzer,M.
  TITLE     Hypertension induces glomerulosclerosis in phospholipase C-epsilon1
            deficiency
  JOURNAL   Am J Physiol Renal Physiol 318 (5), F1177-F1187 (2020)
   PUBMED   32223311
  REMARK    GeneRIF: Plce1 deficiency in mice predisposes to glomerular damage
            secondary to hypertensive insults.
REFERENCE   6  (bases 1 to 9525)
  AUTHORS   Bai,Y., Edamatsu,H., Maeda,S., Saito,H., Suzuki,N., Satoh,T. and
            Kataoka,T.
  TITLE     Crucial role of phospholipase Cepsilon in chemical
            carcinogen-induced skin tumor development
  JOURNAL   Cancer Res 64 (24), 8808-8810 (2004)
   PUBMED   15604236
REFERENCE   7  (bases 1 to 9525)
  AUTHORS   Czyzyk,J., Brogdon,J.L., Badou,A., Henegariu,O., Preston
            Hurlburt,P., Flavell,R. and Bottomly,K.
  TITLE     Activation of CD4 T cells by Raf-independent effectors of Ras
  JOURNAL   Proc Natl Acad Sci U S A 100 (10), 6003-6008 (2003)
   PUBMED   12721365
REFERENCE   8  (bases 1 to 9525)
  AUTHORS   Wu,D., Tadano,M., Edamatsu,H., Masago-Toda,M., Yamawaki-Kataoka,Y.,
            Terashima,T., Mizoguchi,A., Minami,Y., Satoh,T. and Kataoka,T.
  TITLE     Neuronal lineage-specific induction of phospholipase Cepsilon
            expression in the developing mouse brain
  JOURNAL   Eur J Neurosci 17 (8), 1571-1580 (2003)
   PUBMED   12752375
  REMARK    GeneRIF: the induction of phospholipase Cepsilon expression may be
            a specific event associated with the commitment of the neural
            precursor cells to the neuronal lineage.
REFERENCE   9  (bases 1 to 9525)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Aizawa,H., Yuasa,S.,
            Nakajima,D., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: II. The complete nucleotide sequences of 400 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (1), 35-48 (2003)
   PUBMED   12693553
REFERENCE   10 (bases 1 to 9525)
  AUTHORS   Tidhar,A., Reichenstein,M., Cohen,D., Faerman,A., Copeland,N.G.,
            Gilbert,D.J., Jenkins,N.A. and Shani,M.
  TITLE     A novel transgenic marker for migrating limb muscle precursors and
            for vascular smooth muscle cells
  JOURNAL   Dev Dyn 220 (1), 60-73 (2001)
   PUBMED   11146508
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC158905.4, AB076247.1, CD803532.1, CN535034.1, CF537630.1,
            AK122521.1, AK162236.1 and BF466343.1.
            
            On Mar 24, 2007 this sequence version replaced NM_019588.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB076247.1, BC138349.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1248              AC158905.4         132759-134006       c
            1249-1951           AB076247.1         1249-1951
            1952-2115           CD803532.1         409-572
            2116-2630           CN535034.1         129-643
            2631-3163           AB076247.1         2631-3163
            3164-3450           CF537630.1         387-673
            3451-8129           AK122521.1         637-5315
            8130-9447           AK162236.1         2343-3660
            9448-9525           BF466343.1         1-78                c
FEATURES             Location/Qualifiers
     source          1..9525
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 33.16 cM"
     gene            1..9525
                     /gene="Plce1"
                     /gene_synonym="4933403A21Rik; mKIAA1516; Plce; PLCepsilon"
                     /note="phospholipase C, epsilon 1"
                     /db_xref="GeneID:74055"
                     /db_xref="MGI:MGI:1921305"
     exon            1..1256
                     /gene="Plce1"
                     /gene_synonym="4933403A21Rik; mKIAA1516; Plce; PLCepsilon"
                     /inference="alignment:Splign:2.1.0"
     CDS             63..6911
                     /gene="Plce1"
                     /gene_synonym="4933403A21Rik; mKIAA1516; Plce; PLCepsilon"
                     /EC_number="3.1.4.11"
                     /note="PLC-epsilon-1; phosphoinositide phospholipase
                     C-epsilon-1"
                     /codon_start=1
                     /product="1-phosphatidylinositol 4,5-bisphosphate
                     phosphodiesterase epsilon-1"
                     /protein_id="NP_062534.2"
                     /db_xref="CCDS:CCDS37973.1"
                     /db_xref="GeneID:74055"
                     /db_xref="MGI:MGI:1921305"
                     /translation="MTSEEMAASVLIPVTQRKVASAQSVAEERSVKVSDAGIPRARAG
                     RQGALIPPTISQWNKHKEESSRSDLSKVFSIARGELVCDENSNEEGWEENAPDSPENH
                     AMNGNSLVQSHQHQFPRSQLCEARDSVTEDPCLQPGIPSPLERKVLPGIQLEMEDSPM
                     DVSPAGSQPRIMESSGPHSDRNTAVFHFHYEADRTMSDAFHTLSENLILDDCANCVTL
                     PGGQQNKNCMAYACKLVELTRTCGSKNGQVQCEHCTSLRDEYLCFESSCSKADEVCSG
                     GGFCEDGFAHGPAAKTFLNPLEDFSDNCEDVDDFFKSKKERSTLLVRRFCKNDREVKK
                     SVYTGTRAIMRTLPSGCIGPAAWNYVDQKKAGLLWPCGNVMGTLSAMDIRQSGSQRLS
                     EAQWCLIYSAVRRGEEIEDTVGSLLHCSTQLPNSETAHGRIEDGPCLKQCVRDTECEF
                     RATLQRTSIAQYITGSLLEATTSLGARSGLLSSFGGSTGRIMLKERQLGTSMANSNPV
                     PSSSAGISKELIDLQPLIQFPEEVASILTEQEQNIYRRVLPMDYLCFLTRDLSSPECQ
                     RSLPRLKASISESILTSQSGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSY
                     LVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSV
                     EYKKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASGLLKLCPRYSSQEEALEFVADY
                     SGQDNFLQRVGQNGLKNSEKELTVNSIFQVIRSCSRSLEMEEEDSASEGSGSRKNSLK
                     DKARWQFIIGDLLDSENDIFEKSKECDPHGSEESQKAFDHGTELIPWYVLSIQADVHQ
                     FLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWMKPPTASPAGARPKLGVLSNMAEP
                     GKFPSPGNAGVSGLAEGILDLFSVKAVYMGHPGIDIHTVCVQNKLSSMLLSETGVTLL
                     YGLQTTDNRLLHFVAPKHTAEMLFSGLLELTTAVRKIRRFPDQRQQWLRKQYVSLYQE
                     DGRYEGPTLAHAVELFGGRRWSTRNPSPGMSAKNAEKPNMQRNNTLGISTTKKKKKML
                     MRGESGEVTDDEMATRKAKMYRECRSRSGSDPQDVNEQEESEANVITNPPNPLHSRRA
                     YSLTTAGSPNLATGMSSPISAWSSSSWHGRIRGGMQGFQSFMVSDSNMSFVEFVELFK
                     SFSIRSRKDLKDIFDIYSVPCNRSASESAPLYTNLTIEENTSDLQPDLDLLTRNVSDL
                     GLFIKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGE
                     HCTYDEILSIIQKFEPSVSMCHQGLLSFEGFARFLMDKDNFASKNDESRENKKELQLP
                     LSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSIELDCWDGDDGMPIIYHGHT
                     LTTKIPFKEVVEAIDRSAFITSDLPIIISIENHCSLPQQRKMAEIFKSVFGEKLVAKF
                     LFETDFSDDPMLPSPDQLRRKVLLKNKKLKAHQTPVDILKQKAHQLASMQAQAFTGGN
                     ANPPPASNEEEEDEEDEYDYDYESLSDDNILEDRPENKSCADKLQFEYNEEVPKRIKK
                     ADNSSGNKGKVYDMELGEEFYLPQNKKESRQIAPELSDLVIYCQAVKFPGLSTLNSSG
                     SSRGKERKSRKSIFGNNPGRMSPGETAPFNRTSGKGSCEGMRHTWEESSPLSPSTSLS
                     AIIRTPKCYHISSLNENAAKRLCRRGSQKLIQHTAYQLLRTYPAATRIDSSNPNPIMF
                     WLHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVLKPPVLWDKSCPMYQKFSPLERD
                     LDNLDPAIYSLTIISGQNVCPSNSTGSPCIEVDVLGMPLDSCHFRTKPIHRNTLNPMW
                     NEQFLFRVHFEDLVFLRFAVVENNSSAITAQRIIPLRALKRGYRHLQLRNLHNEILEI
                     SSLFINSRRMEENPSGSSMPASLMFNTEERKCSQTHKVTVHGVPGPEPFAVFTINEGT
                     KAKQLLQQVLAVDQDTKCTATDYFLMEEKHFISKEKNECRKQPFQRAVGPEEDIVQIL
                     NSWFPEEGYVGRIVLKPQQETLEEKSIVFDDKEVILSSEEESFFVQVHDVSPEQPRTV
                     IKAPRVSTAQDVIQQTLCKAKYSYSILNNPNPCDYVLLEEVLKDAANKKSSTPKSSQR
                     ILLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKRRGISFASELKKLTKSTKQSRG
                     LPSPPQLVASESVQSKEEKPVGALSSSDTVGYQQ"
     exon            1257..1545
                     /gene="Plce1"
                     /gene_synonym="4933403A21Rik; mKIAA1516; Plce; PLCepsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            1546..1862
                     /gene="Plce1"
                     /gene_synonym="4933403A21Rik; mKIAA1516; Plce; PLCepsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            1863..2008
                     /gene="Plce1"
                     /gene_synonym="4933403A21Rik; mKIAA1516; Plce; PLCepsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            2009..2267
                     /gene="Plce1"
                     /gene_synonym="4933403A21Rik; mKIAA1516; Plce; PLCepsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            2268..2473
                     /gene="Plce1"
                     /gene_synonym="4933403A21Rik; mKIAA1516; Plce; PLCepsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            2474..3149
                     /gene="Plce1"
                     /gene_synonym="4933403A21Rik; mKIAA1516; Plce; PLCepsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            3150..3332
                     /gene="Plce1"
                     /gene_synonym="4933403A21Rik; mKIAA1516; Plce; PLCepsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            3333..3450
                     /gene="Plce1"
                     /gene_synonym="4933403A21Rik; mKIAA1516; Plce; PLCepsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            3451..3559
                     /gene="Plce1"
                     /gene_synonym="4933403A21Rik; mKIAA1516; Plce; PLCepsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            3560..3682
                     /gene="Plce1"
                     /gene_synonym="4933403A21Rik; mKIAA1516; Plce; PLCepsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            3683..3819
                     /gene="Plce1"
                     /gene_synonym="4933403A21Rik; mKIAA1516; Plce; PLCepsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            3820..4058
                     /gene="Plce1"
                     /gene_synonym="4933403A21Rik; mKIAA1516; Plce; PLCepsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            4059..4120
                     /gene="Plce1"
                     /gene_synonym="4933403A21Rik; mKIAA1516; Plce; PLCepsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            4121..4286
                     /gene="Plce1"
                     /gene_synonym="4933403A21Rik; mKIAA1516; Plce; PLCepsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            4287..4394
                     /gene="Plce1"
                     /gene_synonym="4933403A21Rik; mKIAA1516; Plce; PLCepsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            4395..4511
                     /gene="Plce1"
                     /gene_synonym="4933403A21Rik; mKIAA1516; Plce; PLCepsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            4512..4670
                     /gene="Plce1"
                     /gene_synonym="4933403A21Rik; mKIAA1516; Plce; PLCepsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            4671..4800
                     /gene="Plce1"
                     /gene_synonym="4933403A21Rik; mKIAA1516; Plce; PLCepsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            4801..4922
                     /gene="Plce1"
                     /gene_synonym="4933403A21Rik; mKIAA1516; Plce; PLCepsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            4923..5040
                     /gene="Plce1"
                     /gene_synonym="4933403A21Rik; mKIAA1516; Plce; PLCepsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            5041..5172
                     /gene="Plce1"
                     /gene_synonym="4933403A21Rik; mKIAA1516; Plce; PLCepsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            5173..5460
                     /gene="Plce1"
                     /gene_synonym="4933403A21Rik; mKIAA1516; Plce; PLCepsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            5461..5624
                     /gene="Plce1"
                     /gene_synonym="4933403A21Rik; mKIAA1516; Plce; PLCepsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            5625..5886
                     /gene="Plce1"
                     /gene_synonym="4933403A21Rik; mKIAA1516; Plce; PLCepsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            5887..6005
                     /gene="Plce1"
                     /gene_synonym="4933403A21Rik; mKIAA1516; Plce; PLCepsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            6006..6134
                     /gene="Plce1"
                     /gene_synonym="4933403A21Rik; mKIAA1516; Plce; PLCepsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            6135..6344
                     /gene="Plce1"
                     /gene_synonym="4933403A21Rik; mKIAA1516; Plce; PLCepsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            6345..6503
                     /gene="Plce1"
                     /gene_synonym="4933403A21Rik; mKIAA1516; Plce; PLCepsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            6504..6722
                     /gene="Plce1"
                     /gene_synonym="4933403A21Rik; mKIAA1516; Plce; PLCepsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            6723..6945
                     /gene="Plce1"
                     /gene_synonym="4933403A21Rik; mKIAA1516; Plce; PLCepsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            6946..9514
                     /gene="Plce1"
                     /gene_synonym="4933403A21Rik; mKIAA1516; Plce; PLCepsilon"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7346..7351
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Plce1"
                     /gene_synonym="4933403A21Rik; mKIAA1516; Plce; PLCepsilon"
                     /note="hexamer: AATAAA"
     polyA_site      7371
                     /gene="Plce1"
                     /gene_synonym="4933403A21Rik; mKIAA1516; Plce; PLCepsilon"
                     /note="major polyA site"
     regulatory      8003..8008
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Plce1"
                     /gene_synonym="4933403A21Rik; mKIAA1516; Plce; PLCepsilon"
                     /note="hexamer: AATAAA"
     polyA_site      8042
                     /gene="Plce1"
                     /gene_synonym="4933403A21Rik; mKIAA1516; Plce; PLCepsilon"
                     /note="major polyA site"
     regulatory      9419..9424
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Plce1"
                     /gene_synonym="4933403A21Rik; mKIAA1516; Plce; PLCepsilon"
                     /note="hexamer: AATAAA"
     polyA_site      9442
                     /gene="Plce1"
                     /gene_synonym="4933403A21Rik; mKIAA1516; Plce; PLCepsilon"
ORIGIN      
        1 ctggacatga tcgccaggga agggaacaca gaagagtagc tgaatgaacg acagtggtca
       61 agatgacgtc ggaagaaatg gccgcttctg tcctcatccc tgtgactcaa aggaaagtgg
      121 cttctgccca gtcggtggca gaagagagga gtgtaaaggt ctcagatgcg gggattcctc
      181 gggcacgtgc tggcaggcag ggtgcgctca tccctcctac catctcacag tggaacaaac
      241 ataaggaaga gtcgtctaga agtgatttgt ccaaggtctt ctcaatagcg aggggggaac
      301 tagtctgcga tgagaattcc aacgaagagg gctgggagga aaacgcaccg gactccccgg
      361 aaaaccacgc gatgaacggc aacagtttag tgcaaagcca ccagcaccag tttccccgga
      421 gccagctttg tgaagcccgt gactctgtca cagaagaccc gtgtttgcag cctggaattc
      481 cttctcccct ggaaaggaag gtgctccctg gaattcaact ggagatggag gactctccca
      541 tggacgtgag ccctgcggga agtcagccta ggatcatgga gtccagcgga cctcactctg
      601 accgcaacac ggcggtattt cacttccatt atgaagctga caggacaatg tcggatgctt
      661 ttcataccct gtcagaaaac ctgattttgg atgactgtgc aaattgtgtt actcttcctg
      721 gggggcagca aaataaaaat tgcatggcgt atgcttgcaa actggtggag ttgacaagaa
      781 cctgtggaag taagaatggg caagtccagt gtgagcactg tacctcgctg cgcgatgaat
      841 acctgtgctt cgaaagctcc tgctcaaagg ccgatgaggt ctgctcaggt ggtggctttt
      901 gcgaggacgg ctttgctcac ggtcctgctg ccaagacttt tctgaaccct ctggaggatt
      961 tctctgacaa ttgtgaagac gtagacgatt tttttaaaag caaaaaggag cggtccacgt
     1021 tgttagtccg aaggttttgt aaaaatgaca gggaagttaa gaagtctgtg tatactggga
     1081 ccagagccat catgagaact ctgccttctg gctgtattgg gccagctgct tggaattacg
     1141 ttgatcagaa gaaagctggt ctcctatggc cttgtgggaa tgtaatggga actctgtcag
     1201 caatggacat aaggcaaagt gggagccaac gtctgtctga agcccagtgg tgcctgatct
     1261 acagtgccgt gagaagaggg gaagaaatag aagatacggt tggatctctt ctccattgct
     1321 ccacacagct cccaaactct gagacagccc atggaaggat tgaagatggt ccctgcttaa
     1381 agcagtgtgt acgagacact gaatgtgaat tccgtgctac cctccaaagg acatccatag
     1441 ctcagtacat caccggttct cttctagagg caactacatc tttaggagca agaagtggcc
     1501 ttctcagttc cttcggagga tccactggac gaataatgct gaaagaacgc caactgggca
     1561 cctctatggc caattccaat cctgttcctt caagttcagc tggaatcagc aaggagctga
     1621 ttgatctcca gcccctcatt cagttcccgg aggaagtagc cagcatctta acagagcagg
     1681 aacagaacat ttaccgcagg gtcctgccca tggactacct ctgcttctta acccgggact
     1741 tgagttcacc tgaatgccag agatcactgc cccgtctcaa agcatccatc tcagaatcca
     1801 tcctcacgtc tcaaagtgga gaacacaatg cccttgaaga ccttgtgatg agatttaacg
     1861 aggtgagctc ctgggtaaca tggctgatcc tcacagcagg ctccatggag gagaagcggg
     1921 aagtcttttc ttatttggtg catgtggcca aatgctgctg gaacatgggc aactacaacg
     1981 ctgtcatgga gttcctggct ggcctcaggt caaggaaagt tctaaagatg tggcagttca
     2041 tggaccagtc tgacattgag accatgcgga gcctgaagga tgccatggcc cagcatgagt
     2101 cttccgtgga gtacaagaag gtagtgaccc gagccttgca catccccggc tgcaaagtgg
     2161 tccccttctg tggggtgttc ctgaaggagc tctgtgaggt gcttgatggt gcctctggcc
     2221 tcctgaagct ctgcccacgg tacagctccc aagaagaagc tctggagttt gtagccgatt
     2281 acagtggaca agataacttc ttacaacgag tgggacagaa tggcttaaag aattcagaga
     2341 aggagctcac ggtcaacagc atctttcagg tcatccggag ctgcagccga agtttggaga
     2401 tggaggagga agacagtgcc agtgaaggga gcggctctag gaaaaactcc ctgaaggaca
     2461 aagcccgatg gcagtttata attggagatt tgctggattc agaaaatgac atctttgaga
     2521 agtccaaaga gtgtgacccc catggatccg aggagtcaca gaaagccttt gaccatggga
     2581 cagagcttat cccatggtac gtgctgtcca tccaagcaga tgtgcaccag ttcctattgc
     2641 agggggccac agtcatccac tatgaccagg acacacatct ctcggcccgc tgctttctcc
     2701 agttgcagcc tgacaatagc acgttgacct ggatgaaacc tcccactgcc tccccagcag
     2761 gcgccagacc caagctcggt gtgcttagta acatggccga gcctggcaag ttcccatccc
     2821 caggcaacgc cggggtgagc ggcctggcag agggaattct ggatctgttc tcagtgaagg
     2881 ctgtgtacat gggacaccct ggcattgata tacacactgt gtgtgtccag aacaaactta
     2941 gtagcatgct tctatcggag acgggggtca cactgctcta tgggcttcag accacggaca
     3001 atagattact gcactttgtg gcgccaaagc acacggctga gatgcttttc agtggattgc
     3061 tggaactcac tacagctgtg agaaagataa ggaggttccc tgaccaacga cagcagtggc
     3121 ttcggaaaca gtatgtcagc ctctatcagg aggatggtcg gtatgaaggc ccaaccttgg
     3181 cacatgctgt ggagttgttt ggtggtaggc ggtggagcac tagaaacccc agccctggaa
     3241 tgtcagcaaa gaatgctgag aagcccaaca tgcagagaaa caacaccctg ggcataagca
     3301 ccaccaagaa aaagaagaaa atgctcatga ggggagagag tggagaggtg acggacgatg
     3361 agatggctac ccgcaaggcc aaaatgtaca gagagtgtcg cagccggagc ggctctgatc
     3421 ctcaagacgt taacgaacaa gaagagtcgg aggccaatgt catcactaac cctcccaacc
     3481 ccctccattc cagaagagcc tactctttga ccacggctgg gtcccccaac ttggctaccg
     3541 ggatgtcatc tcccatcagt gcctggagca gcagcagctg gcatgggcga atccgaggag
     3601 gaatgcaagg cttccagagc ttcatggtct cagacagtaa catgagtttc gttgaattcg
     3661 tcgagctgtt caaatcattc agcatcagga gccgcaagga cttgaaggac atctttgaca
     3721 tctactctgt accctgcaat cggtctgcct cggagtcagc cccactctac accaacctga
     3781 caatcgaaga aaataccagc gatcttcagc ctgacctaga tttgctgact agaaacgtct
     3841 ccgacctggg gctgttcatt aaaagtaaac agcagctttc tgacaaccag aggcagatat
     3901 ctgatgctat cgctgctgca agcatcgtga ccaatggtac tgggattgaa agcacgtccc
     3961 tgggcatatt tggggtcggc atactccagc tcaatgactt cctggtgaat tgccaaggag
     4021 aacactgcac atatgatgaa atcctgagca tcatccagaa atttgagcct agcgtcagca
     4081 tgtgtcatca aggcctactg tcatttgaag ggtttgcaag gtttctgatg gataaagaca
     4141 atttcgcctc aaaaaatgat gagtcacggg agaacaagaa agaactgcag ctacccctct
     4201 cctactatta tatagaatct tcacacaaca cctacctcac cggccatcag ctcaaggggg
     4261 agtcctctgt ggagctctat agccaggtcc tcctgcaagg atgtagaagc atagagctgg
     4321 actgctggga cggggatgat gggatgccca tcatctatca tgggcataca ctgacaacca
     4381 agatcccctt caaggaagtg gtggaagcca tcgatcgcag tgccttcatc acctcagacc
     4441 tgccaatcat catatccatt gagaaccatt gttcattgcc tcaacaacga aaaatggcag
     4501 aaattttcaa gagtgtgttt ggagaaaagc tggtggctaa gttcttattc gagaccgatt
     4561 tctcagatga cccaatgctt ccctcacctg atcaacttag aaggaaagta ctccttaaaa
     4621 acaagaagct aaaggcccat cagacacctg tcgacatcct aaaacaaaag gctcatcagt
     4681 tagcatccat gcaagcacag gctttcaccg gtgggaatgc caacccgcca cctgccagta
     4741 atgaggaaga ggaagatgag gaggatgaat atgattacga ttacgagtcc ctgtccgatg
     4801 acaatattct ggaagacaga cctgagaaca aatcatgcgc tgacaagctt cagtttgagt
     4861 acaatgaaga ggtccccaag aggataaaga aagcagacaa ctcctctggc aataagggaa
     4921 aggtgtatga catggaactg ggagaggagt tttaccttcc tcagaacaaa aaggaaagca
     4981 ggcagattgc accggagctt tctgacctcg tcatttattg ccaggcagta aaatttccag
     5041 gcctgtcaac tctaaattca tctggctcta gcagagggaa agaaaggaaa agcaggaagt
     5101 ccatttttgg caacaatcct ggcagaatga gccccgggga gacagcgcca tttaacagaa
     5161 catctggaaa aggttcctgt gaagggatgc gacacacctg ggaggaatct tctccactca
     5221 gcccaagcac atccctcagc gctatcatca ggactcctaa atgctatcac atctcctcac
     5281 tgaacgagaa cgccgccaaa cgtctgtgcc gcaggggttc tcagaaactg atccagcaca
     5341 ccgcttatca gctgctgagg acctacccgg cagcgaccag aatcgactcc tccaatccca
     5401 accccatcat gttctggctc catgggatac agcttgtagc tctaaactac caaaccgatg
     5461 accttccttt acatttaaac gccgccatgt ttgaggcgaa tggtgggtgt ggttatgttt
     5521 tgaagccccc tgtcctgtgg gataaaagct gtcccatgta ccagaagttt tctccactgg
     5581 aaagagacct agacaacctg gatcctgcta tctattcttt aactatcatc tctggccaga
     5641 atgtgtgccc tagtaacagc accggaagtc catgcattga agtcgacgtc ttgggcatgc
     5701 ccctggacag ctgccatttc cgcacaaaac ccatccatcg aaacactcta aaccccatgt
     5761 ggaatgagca gtttctcttc cgggttcact ttgaagatct tgtatttctt cgttttgcag
     5821 tcgtggaaaa caacagttcg gcgataacgg ctcagagaat cattccactc agagctttga
     5881 aacgaggata tcgacatctt cagctgcgga acctccacaa tgaaatcttg gaaatctcta
     5941 gcttattcat aaacagcaga aggatggaag aaaacccctc tggcagttcc atgccagcct
     6001 cgttgatgtt taatactgaa gagaggaaat gttctcagac tcacaaagtc acagtgcatg
     6061 gtgtcccagg tccagagccc tttgctgttt tcactataaa cgaaggcacc aaggcaaagc
     6121 agcttctcca acaggttttg gcagttgacc aagacaccaa atgtactgcc acagactatt
     6181 ttttgatgga agagaagcat tttatatcta aggaaaagaa tgaatgcagg aaacaaccat
     6241 tccagagagc tgtgggtcca gaagaagata ttgtgcagat tttaaacagt tggttcccag
     6301 aagaaggcta cgtaggcagg attgtcttaa agccccaaca ggaaactcta gaagagaaaa
     6361 gcattgtctt cgacgacaaa gaggtgatct tgagctcaga ggaggagagt ttctttgtgc
     6421 aagtgcatga tgtctctcca gagcaacctc gaactgtcat caaagcccca cgtgtcagca
     6481 ctgcacagga tgtaattcag cagaccttat gcaaagccaa atattcctac agcatcctga
     6541 acaaccccaa cccatgtgac tatgtgcttt tggaagaggt gctgaaagat gcagctaata
     6601 agaagtcttc taccccaaag tcctcgcaga ggatcctctt ggaccaagaa tgtgtgtttc
     6661 aagcgcaaag caagtggaag ggtgcgggaa agttcatact taagctaaag gagcaggtgc
     6721 aggcatcccg agaagacaaa aggaggggta tctcctttgc aagtgaactc aagaagctca
     6781 ctaagtccac taagcagtct cgagggctcc catcacctcc tcagcttgtg gcctcagaaa
     6841 gtgttcaaag caaggaggag aaacctgtgg gtgccttgtc ctctagtgac acagtgggtt
     6901 accagcagtg agcaagtgcg gcatatttgt tccagggata tgaaggggga ggtccttgac
     6961 gaagagctat caagaacgaa cgtgggggga agaaaaatac ttcacctcca tcacatttaa
     7021 actggaaatg gatgatttct caactgcaac cctcttcaca cctgatggat gtgaggttcg
     7081 tggagaggac aagcaaaaag caaaggcaca ggtctgccaa ccaacgtcct gaagggtaaa
     7141 gcagggacac ccaggccatt gcccggagca ttacaggtgg agaggccacc cccaggcttc
     7201 aggcaacgag cttcacctgt aaaggaccag agacggtaaa cattttaggg ttggggcccc
     7261 atggcctctg tggcactgag ttacctcagc cgctgagtgc aaatgcagct gctgacaaca
     7321 ggtacaagaa tgaacatgac tggacaataa agctttattt gtggacactt acattggaac
     7381 ctcagagagt tttcacgggt cacgcatcct tcaagtgttt caatcactta aaattgtaaa
     7441 aatattttct tagttcaagg gtcacacaaa aacacatgaa tttggctcaa ggaccagctt
     7501 gctgacccca acttgggaga actgggaacc aaaggtatat agaggttagg aaacctcaaa
     7561 aattggccat gggtttaatg agaagtggaa gtctgggggc attggaaatg taaatatgtc
     7621 ctgtaagatt tattaacaat tattcaaatt agttcctata ttgtgtaccc aggttcgctc
     7681 tttgggctga ctttcctcaa acagaaaaaa attttttttt ccaaaagctc ccgttcatac
     7741 gtccacagac tgttctgttt tcagttctca ccaagtgaat ctagaatgat gtacaattca
     7801 gtgtctatgg agttgtgttg tcttcagaag aagcctaaat catttctaag aagcaaaagt
     7861 agactgggtt ggcgtttaag gtagatttat ttattttcca atgtttatac tgacacttgt
     7921 gtatttatta gatattacct ttcttgatat tgctatgggt gacaagcatt agtggttcga
     7981 tctgtttcaa gacatcctaa aaaataaatc atatgagcat gagactcggg tagagatgca
     8041 gagccatagt gtgatcttca tttcagattc cctgaggagc cgtggctccg agtctcactg
     8101 gccatgtgat ctaagtcatg ttaaaaccta cttcacaaaa caatcagcag agtgcaatgg
     8161 actcttctca tcctcggggt gaggcaaatg gtgactcatc agactgaagt acgtgttcgt
     8221 ccagagtcaa gcctatattt catctttcaa ttgtccctgt tccgcttgaa gatattcttc
     8281 ttcaagttag aaaacccact aaaaatcctg acttttgctt tcgagacttt cctttaccaa
     8341 ttacagaagg aataaacaac cccttctttg cacattctaa aaagagacat gtcaaaaata
     8401 caccacacag cttccctatc actggaagac tctggctgca ctgtctttct cttgccaaga
     8461 gaactcagac tattctcatt tggcagttac acagcactaa caggttccct ttatattaaa
     8521 tactaggtct gtaccaagcg cacagcgctg cttcggtttt aaaacatatt ctggagccat
     8581 tttatcaatt ttctgtaaat taaagaggcc aaatactttg ggttaccttg ggttgaaaat
     8641 caaattactg gtctaaccag ccagagccaa acaaaacctg caggtaataa gataccgagc
     8701 caggctggtt taccctaccc ttgtggtcct ccttataata gatggagatt tgatacagaa
     8761 ctgaaataga ccccatattc aaatgctggt gtgcatccca gtttaggtta aaggacatac
     8821 tctactaaaa ttgagtctcc aagactttaa gcagaagaga ttactaaaca tatgcaaaga
     8881 gggccatcga gatgacccag aggtggccct tgccacccac gctttgatga cctcagtttg
     8941 ataccggaac caatggaaaa gtaaaaggag aaaactgatt ccataaaatt atctatcctc
     9001 tgtctccaca ggtgccccca catcatatag catacacata caaaaataat aataaatgaa
     9061 ataaaaaatt taaagtatta acagcttgtc acaggaatgt gttacttaat gaggacgtat
     9121 tttaagaaat gcattggtag ggaacttcat gttgtatgaa tgtcagaata tacctacaca
     9181 agctaagatg gctactgtgt cacggaacaa ggagcttttt ggctttatta tacattatgg
     9241 ggtcacggtt ctgttccata gcaccccaca cagcagttta tccatgatca gagcaccact
     9301 gtaaggtaca tgactgcatt acaactcctt agcataatat ggtgatgttt tgactgaagg
     9361 cttatctgaa ttatgggatg taactggttt atcaaatgaa agaaataacc ctattcataa
     9421 taaattagca gggccactag aattgcgccc accaccattt tatacttttt agaatacaag
     9481 aaatctggat aacaaaaatc ttaaaccaaa aaaaaaaaaa aaaaa
//
