LOCUS       NM_001355153            5438 bp    mRNA    linear   ROD 11-SEP-2024
DEFINITION  Mus musculus phospholipase C, beta 4 (Plcb4), transcript variant 1,
            mRNA.
ACCESSION   NM_001355153 XM_006498940
VERSION     NM_001355153.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5438)
  AUTHORS   Li,G., Chen,L., Jiang,Z. and Yau,K.W.
  TITLE     Coexistence within one cell of microvillous and ciliary
            phototransductions across M1- through M6-IpRGCs
  JOURNAL   Proc Natl Acad Sci U S A 120 (52), e2315282120 (2023)
   PUBMED   38109525
REFERENCE   3  (bases 1 to 5438)
  AUTHORS   Chen,X., Qin,Y., Zhang,Y., Yang,X., Xing,Z., Shen,Y., Cheng,J.,
            Yeh,E.T.H., Wu,H. and Qi,Y.
  TITLE     SENP2-PLCbeta4 signaling regulates neurogenesis through the
            maintenance of calcium homeostasis
  JOURNAL   Cell Death Differ 29 (2), 337-350 (2022)
   PUBMED   34465891
  REMARK    GeneRIF: SENP2-PLCbeta4 signaling regulates neurogenesis through
            the maintenance of calcium homeostasis.
REFERENCE   4  (bases 1 to 5438)
  AUTHORS   Sheng,Y., Chen,L., Ren,X., Jiang,Z. and Yau,K.W.
  TITLE     Molecular determinants of response kinetics of mouse M1
            intrinsically-photosensitive retinal ganglion cells
  JOURNAL   Sci Rep 11 (1), 23424 (2021)
   PUBMED   34873237
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5438)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5438)
  AUTHORS   Jiang,H., Lyubarsky,A., Dodd,R., Vardi,N., Pugh,E., Baylor,D.,
            Simon,M.I. and Wu,D.
  TITLE     Phospholipase C beta 4 is involved in modulating the visual
            response in mice
  JOURNAL   Proc Natl Acad Sci U S A 93 (25), 14598-14601 (1996)
   PUBMED   8962098
REFERENCE   7  (bases 1 to 5438)
  AUTHORS   Lyu,M.S., Park,D.J., Rhee,S.G. and Kozak,C.A.
  TITLE     Genetic mapping of the human and mouse phospholipase C genes
  JOURNAL   Mamm Genome 7 (7), 501-504 (1996)
   PUBMED   8672127
REFERENCE   8  (bases 1 to 5438)
  AUTHORS   Amson,R.B., Nemani,M., Roperch,J.P., Israeli,D., Bougueleret,L., Le
            Gall,I., Medhioub,M., Linares-Cruz,G., Lethrosne,F., Pasturaud,P.,
            Piouffre,L., Prieur,S., Susini,L., Alvaro,V., Millasseau,P.,
            Guidicelli,C., Bui,H., Massart,C., Cazes,L., Dufour,F.,
            Bruzzoni-Giovanelli,H., Owadi,H., Hennion,C., Charpak,G.,
            Telerman,A. et al.
  TITLE     Isolation of 10 differentially expressed cDNAs in p53-induced
            apoptosis: activation of the vertebrate homologue of the drosophila
            seven in absentia gene
  JOURNAL   Proc Natl Acad Sci U S A 93 (9), 3953-3957 (1996)
   PUBMED   8632996
REFERENCE   9  (bases 1 to 5438)
  AUTHORS   Martelli,A.M., Lach,S., Grill,V., Gilmour,R.S., Cocco,L.,
            Narducci,P. and Bareggi,R.
  TITLE     Expression and immunohistochemical localization of eight
            phospholipase C isoforms in adult male mouse cerebellar cortex
  JOURNAL   Acta Histochem 98 (2), 131-141 (1996)
   PUBMED   8739298
REFERENCE   10 (bases 1 to 5438)
  AUTHORS   Hempel,W.M. and DeFranco,A.L.
  TITLE     Expression of phospholipase C isozymes by murine B lymphocytes
  JOURNAL   J Immunol 146 (11), 3713-3720 (1991)
   PUBMED   2033248
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL929052.10, AL935301.8 and AL928798.20.
            
            On Sep 9, 2017 this sequence version replaced XM_006498940.2.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660817.30364.1,
                                           SRR10223969.3166.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849387
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-184               AL929052.10        55585-55768
            185-242             AL935301.8         52233-52290
            243-299             AL935301.8         115952-116008
            300-398             AL928798.20        7472-7570
            399-479             AL928798.20        37588-37668
            480-539             AL928798.20        38439-38498
            540-683             AL928798.20        40190-40333
            684-763             AL928798.20        60044-60123
            764-817             AL928798.20        62338-62391
            818-899             AL928798.20        68349-68430
            900-1000            AL928798.20        69299-69399
            1001-1058           AL928798.20        70036-70093
            1059-1167           AL928798.20        77135-77243
            1168-1378           AL928798.20        80301-80511
            1379-1472           AL928798.20        82082-82175
            1473-1552           AL928798.20        84435-84514
            1553-1637           AL928798.20        85004-85088
            1638-1728           AL928798.20        88144-88234
            1729-1824           AL928798.20        88731-88826
            1825-1925           AL928798.20        91808-91908
            1926-2067           AL928798.20        97114-97255
            2068-2152           AL928798.20        98006-98090
            2153-2277           AL928798.20        98410-98534
            2278-2329           AL928798.20        100483-100534
            2330-2432           AL928798.20        101847-101949
            2433-2597           AL928798.20        103001-103165
            2598-2802           AL928798.20        106188-106392
            2803-2891           AL928798.20        112122-112210
            2892-3042           AL928798.20        113065-113215
            3043-3092           AL928798.20        117569-117618
            3093-3158           AL928798.20        117926-117991
            3159-3274           AL928798.20        124383-124498
            3275-3350           AL928798.20        128766-128841
            3351-3526           AL928798.20        130223-130398
            3527-3628           AL928798.20        132648-132749
            3629-3686           AL928798.20        137429-137486
            3687-3773           AL928798.20        137912-137998
            3774-3810           AL928798.20        141025-141061
            3811-5438           AL928798.20        143055-144682
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 66.64 cM"
     gene            1..5438
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /note="phospholipase C, beta 4"
                     /db_xref="GeneID:18798"
                     /db_xref="MGI:MGI:107464"
     exon            1..184
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            185..242
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            243..299
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            300..398
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             315..3899
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /EC_number="3.1.4.11"
                     /note="isoform a is encoded by transcript variant 1"
                     /codon_start=1
                     /product="1-phosphatidylinositol 4,5-bisphosphate
                     phosphodiesterase beta-4 isoform a"
                     /protein_id="NP_001342082.1"
                     /db_xref="GeneID:18798"
                     /db_xref="MGI:MGI:107464"
                     /translation="MAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEF
                     GFFLTWKSEGKEGQVLECSLINSIRQAAIPKDPKILAALEAVGKSENDLEGRILCVCS
                     GTDLVNIGFTYMVAENPEVTKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFLTNT
                     TGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFYELTQKICP
                     RTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYE
                     PDEELKKKGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTG
                     RQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAI
                     KETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPS
                     PNDLKRKILIKNKRLKPEVEKKQLEALKSMMEAGESAAPASILEDDNEEEIESADQEE
                     EAHPEYKFGNELSADDYSHKEAVANSVKKGLVTVEDEQAWMASYKYVGATTNIHPYLS
                     TMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYP
                     KGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRR
                     PDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRT
                     RMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRH
                     ISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMG
                     IETSDIADVPSDTSKNDKKGKANPAKANVTPQSSSELRPTTTAALGSGQEAKKGIELI
                     PQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEK
                     STHEKILEKAMKKKGGSNCLEIKKETEIKIQTLTTDHKSKVKEIVAQHTKEWSEMINT
                     HSAEEQEIRDLHLSQQCELLRKLLINAHEQQTQQLKLSHDRESKEMRAHQAKISMENS
                     KAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEF
                     LEKQNEQLLKSCHAVSQTQGEGDAADGEIGSRDGPQTSNSSMKLQSAN"
     exon            399..479
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            480..539
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            540..683
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            684..763
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            764..817
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            818..899
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            900..1000
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1001..1058
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1059..1167
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1168..1378
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1379..1472
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1473..1552
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1553..1637
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1638..1728
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1729..1824
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1825..1925
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1926..2067
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2068..2152
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2153..2277
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2278..2329
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2330..2432
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2433..2597
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2598..2802
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2803..2891
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2892..3042
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3043..3092
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3093..3158
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3159..3274
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3275..3350
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3351..3526
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3527..3628
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3629..3686
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3687..3773
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3774..3810
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3811..5438
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5420..5425
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /note="hexamer: AATAAA"
     polyA_site      5438
                     /gene="Plcb4"
                     /gene_synonym="A930039J07Rik; C230058B11Rik"
                     /note="major polyA site"
ORIGIN      
        1 agggggtggc ggcgcgcgag cggctcagga gccgcgccgc aaacaggctc tcacacgcgc
       61 gcacacacac acacacacac acacacacac acacagacac tcacacgcgc gcgcgcgaac
      121 acagcggggc tcccggaggc ggcggtggcc gcatccccgg cagcgactcg cctggaagtg
      181 acaggagcct gctttgtgcg tgctgtcaac tacctgggag gattctcgac acagcagcct
      241 ggggactgtg caggatttga tcgggtagac ctcccttgct tgctgctccc agctgcctgg
      301 tcttgaatac catcatggcc aaaccttacg aatttaactg gcagaaggaa gtgccctctt
      361 tcttgcaaga aggagcagtt tttgacagat acgaagagga atcttttgtg tttgagccca
      421 actgcctctt caaagtagat gaattcggct tcttcctgac gtggaagagt gaaggcaagg
      481 aaggacaagt gctagaatgt tccctcatca acagtattcg ccaagcagcc ataccaaagg
      541 atcccaaaat cctggctgct ctcgaagctg ttggaaaatc tgaaaatgat ctggaagggc
      601 ggatattgtg tgtctgcagc ggcacggatc tggtgaatat cggcttcact tacatggtgg
      661 ctgaaaatcc agaagtaact aagcaatggg tagaaggcct gagatcgatc attcacaact
      721 tcagggcaaa caacgtcagt ccgatgacat gcctcaagaa acactggatg aaactggcct
      781 ttctgaccaa cacaactggt aaaatcccag tgaggagtat cactagaacc ttcgcatcag
      841 ggaaaacaga aaaggtgatc tttcaagccc tcaaggaact aggtcttccc agtggaaaga
      901 atgatgaaat tgaacctgct gcatttactt atgaaaagtt ctatgaactg acacaaaaga
      961 tttgtcctcg gacagatata gaagatcttt ttaaaaaaat caatggagac aaaactgatt
     1021 atttaacggt agaccaatta gtgagctttc taaacgaaca tcagcgagat cctcggctga
     1081 atgaaatttt attcccattt tatgatgcta aaagagcaat gcagatcatt gaaatgtatg
     1141 agcctgatga agagctgaag aaaaaaggcc tcatatccag tgatggattc tgcagatatc
     1201 tgatgtcaga tgaaaatgcc cctgtcttct tagatcgctt agaactttac caggagatgg
     1261 accacccgct ggctcattac ttcatcagtt cctcccacaa cacctatctc actggccggc
     1321 aatttggagg aaagtcttca gtggaaatgt acagacaagt tctcctggct ggttgcaggt
     1381 gtgttgaact tgactgttgg gatggaaaag gtgaagatca ggaaccgata ataactcacg
     1441 gaaaagcaat gtgtacagac atccttttta aggatgtaat ccaggccatc aaggaaacgg
     1501 cgtttgtcac atcagaatac cctgtcattc tctccttcga aaaccactgc agcaaatatc
     1561 aacagtacaa gatgtccaag tattgtgaag atctatttgg ggatctcctg ttgaaacaag
     1621 cacttgagtc gcatccactt gaaccaggaa ggcccttgcc gtctcctaat gacctcaaaa
     1681 gaaaaatact catcaagaat aagaggctga agcctgaagt tgaaaagaaa cagcttgaag
     1741 ctttgaaaag catgatggaa gctggagagt cagccgcccc agctagcatc ttggaagacg
     1801 acaatgaaga ggaaatagaa agtgctgatc aagaggaaga agcccaccct gaatacaaat
     1861 ttggaaatga actttctgcc gatgactaca gtcacaagga agcggttgca aacagcgtca
     1921 agaagggcct ggtcaccgta gaggatgagc aagcatggat ggcatcttat aaatacgtag
     1981 gtgctaccac gaacatccat ccgtacttgt ccacgatgat caactatgcc cagcccgtga
     2041 agtttcaagg tttccacgtg gctgaagagc gcaatattca ctataacatg tcttctttta
     2101 acgagtcggt tggccttggc tacttgaaga cgcacgcgat tgagtttgta aattacaata
     2161 agcgacaaat gagccgcatt taccccaagg gaggccgagt tgattccagt aattacatgc
     2221 ctcagatttt ctggaacgct ggttgccaga tggtttcact gaactatcaa accccagatt
     2281 tagcgatgca attgaatcaa ggaaaatttg agtataatgg atcatgcggg taccttctca
     2341 agccagattt catgaggcgg cctgatcgga catttgaccc cttctctgaa acccctgtgg
     2401 acggggttat tgcagccacg tgctcagtgc aggttatatc agggcagttc ctctcagata
     2461 agaagatcgg gacatacgtg gaagtcgata tgtacgggct gcccaccgac accatacgga
     2521 aagagttccg aacccgcatg gttatgaaca atggactcaa cccagtgtat aatgaagaat
     2581 cgtttgtgtt tcgaaaggtg atcctgcctg acctagctgt cctgagaatc gcagtctacg
     2641 atgacaacaa caagctaatt ggccagagga tccttccttt ggatggtctc caagcaggct
     2701 accgacacat ctccctgaga aacgagggaa acaaaccatt atcactgcca acaattttct
     2761 gcaatattgt tcttaaaaca tacgtgcctg atggatttgg agatattgtg gatgctttat
     2821 ccgatccaaa gaaatttctt tcaatcacag agaagagagc agaccaaatg agagcaatgg
     2881 gcattgaaac tagtgacata gcagatgtgc ccagtgacac ttccaaaaat gacaagaaag
     2941 gcaaggccaa cccagccaaa gcgaacgtga cccctcagag cagctctgag ctcagaccaa
     3001 ccaccacagc cgccctgggc tctggccagg aagccaagaa aggtattgaa cttatccctc
     3061 aagtgaggat agaagattta aagcaaatga aggcttactt gaagcattta aagaaacaac
     3121 agaaggagtt aaactcttta aagaagaaac atgcaaagga gcacagtacc atgcagaagt
     3181 tacactgcac acaagttgac aaaatcgtgg cccagtatga caaagagaag tcgactcatg
     3241 agaaaatcct agagaaggcg atgaagaaga aggggggaag taattgtctt gaaataaaaa
     3301 aagaaacaga aattaaaatt cagaccctga caacggatca caaatctaag gtcaaagaga
     3361 ttgtggccca gcacacaaag gagtggtcag aaatgatcaa cactcacagt gcggaggagc
     3421 aggaaatccg ggatctgcac ctgagccagc agtgtgagct gctgagaaag ctgctcatca
     3481 atgctcatga gcagcagacc cagcagctga aactctccca tgacagggaa agcaaggaga
     3541 tgagagccca tcaggctaag atttctatgg aaaatagcaa ggccatcagt caagataaat
     3601 ctatcaagaa caaggcagaa cgggaaaggc gagtcaggga gttgaacagc agcaacacta
     3661 agaagttcct agaagaaaga aagagactgg cgatgaagca gtcaaaagaa atggatcagt
     3721 tgaaaaaagt ccagctggag cacctagaat tcctagagaa acagaacgag cagcttttga
     3781 aatcctgtca tgcagtgtcc caaacacaag gcgaaggaga tgcagcagat ggtgaaattg
     3841 gaagccgaga tggaccgcag accagcaaca gtagtatgaa actccagagt gcaaactgaa
     3901 gctggaagac catgcaacac cagaagatca tgcccagctt ccaaacacag actccacaga
     3961 caaaagctgt tttctttgtg tttcttttat gtatagacac ggtgttacct gaaaccacat
     4021 ggactgggga tgaccaaacg ctcttatatt taacaatttg agtattgaat ttctttggcc
     4081 aaccaaaagt agctatgtac acatacacac acacacatac acacacacac acagagagag
     4141 agagagagag agagagagag agagagagag agagagagaa atcccctatt cctgacaggc
     4201 agagttgaat catgatatat gacttaaaca tgtttgctat gagacagcat cacaagccag
     4261 tgggcttggt gataacaact ctgctttgtg gtgcattagg acatgtttga gctgctgctg
     4321 ctgcaaaaca aaacaaaaca aaaaaattag tgcattagta acttaatagt aagcgcatgc
     4381 actagaagaa acacctctgt ctccagttca ttagcagaag tggccgtctg ctatacaaat
     4441 cacttcgcag aattttctac acctaagtta cctcatcagt aagtgccatg tctttaccat
     4501 gccataagat gctaaccttc ctgtaaatgt tgcagaaatc gttaggacaa tagagacaca
     4561 aaacggtgta tctgtgccaa gctcatcaat gctcccaggg ggactcggca ggctcgtttc
     4621 agaaagttta cagctgaccc tgctttgtag gaattgcttc tgccgattcc acatattgca
     4681 attcactcta cactataagg atcgtcaagt ggttattggc aaatgtttgt aaatgtgacc
     4741 atgtacaaag tatttatact ccttaattct cacagttata gctcaaagtc atctaaatat
     4801 taccacacga caagactagt gagcaggaat catagacgta atgttttgca tgatgcacaa
     4861 gcaccaatta tgactaaccc attcaaacac agttaaccta acaccaaata aatactggtt
     4921 aagtaaatgt acggcacaga atattatttg actatcaaga cttttagcat aatgaaaaag
     4981 tctatatata tgtgtatatg aattatgtgg gcattcttga tacttcaagt tctagtttga
     5041 aaaatacata actaatttaa ttttacacaa aaatatttat gcagattttc agactttcat
     5101 atcaggaaat aaccttttta tgtctgttaa atatccaaac aatttgctac cgtgttaatc
     5161 tgcatggtct tctcgcctgc tgtagttgcc ttgcaggtgt ctcataaagt ggaccgatgg
     5221 gatggagtca tgctgcccaa actcagagga gcatgcaggg atccggtagc ctagcaagag
     5281 tcagattcct gattttagaa tacaaaatgc caacttaatt gttgtatatg gttgtacaaa
     5341 tagattctgt accttcctct gaagagggca gagaagatta tgataccatg aaggctaaaa
     5401 ttcaatgtac aacttctcta ataaaaaatg aaaacaca
//
