LOCUS       NM_177568               5145 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus phospholipase C, beta 2 (Plcb2), transcript variant 1,
            mRNA.
ACCESSION   NM_177568 XM_110361
VERSION     NM_177568.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5145)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5145)
  AUTHORS   Ohmoto,M., Jyotaki,M., Yee,K.K. and Matsumoto,I.
  TITLE     A Transcription Factor Etv1/Er81 Is Involved in the Differentiation
            of Sweet, Umami, and Sodium Taste Cells
  JOURNAL   eNeuro 10 (4) (2023)
   PUBMED   37045597
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5145)
  AUTHORS   Phoenix,K.N., Yue,Z., Yue,L., Cronin,C.G., Liang,B.T.,
            Hoeppner,L.H. and Claffey,K.P.
  TITLE     PLCbeta2 Promotes VEGF-Induced Vascular Permeability
  JOURNAL   Arterioscler Thromb Vasc Biol 42 (10), 1229-1241 (2022)
   PUBMED   35861069
  REMARK    GeneRIF: PLCbeta2 Promotes VEGF-Induced Vascular Permeability.
REFERENCE   4  (bases 1 to 5145)
  AUTHORS   Golden,E.J., Larson,E.D., Shechtman,L.A., Trahan,G.D., Gaillard,D.,
            Fellin,T.J., Scott,J.K., Jones,K.L. and Barlow,L.A.
  TITLE     Onset of taste bud cell renewal starts at birth and coincides with
            a shift in SHH function
  JOURNAL   Elife 10, e64013 (2021)
   PUBMED   34009125
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5145)
  AUTHORS   Hsu,C.C., Seta,Y., Matsuyama,K., Kataoka,S., Nakatomi,M.,
            Toyono,T., Gunjigake,K.K., Kuroishi,K.N. and Kawamoto,T.
  TITLE     Mash1-expressing cells may be relevant to type III cells and a
            subset of PLCbeta2-positive cell differentiation in adult mouse
            taste buds
  JOURNAL   Cell Tissue Res 383 (2), 667-675 (2021)
   PUBMED   32960355
  REMARK    GeneRIF: Mash1-expressing cells may be relevant to type III cells
            and a subset of PLCbeta2-positive cell differentiation in adult
            mouse taste buds.
REFERENCE   6  (bases 1 to 5145)
  AUTHORS   Kim,D., Jun,K.S., Lee,S.B., Kang,N.G., Min,D.S., Kim,Y.H.,
            Ryu,S.H., Suh,P.G. and Shin,H.S.
  TITLE     Phospholipase C isozymes selectively couple to specific
            neurotransmitter receptors
  JOURNAL   Nature 389 (6648), 290-293 (1997)
   PUBMED   9305844
REFERENCE   7  (bases 1 to 5145)
  AUTHORS   Jiang,H., Kuang,Y., Wu,Y., Xie,W., Simon,M.I. and Wu,D.
  TITLE     Roles of phospholipase C beta2 in chemoattractant-elicited
            responses
  JOURNAL   Proc Natl Acad Sci U S A 94 (15), 7971-7975 (1997)
   PUBMED   9223297
REFERENCE   8  (bases 1 to 5145)
  AUTHORS   Lyu,M.S., Park,D.J., Rhee,S.G. and Kozak,C.A.
  TITLE     Genetic mapping of the human and mouse phospholipase C genes
  JOURNAL   Mamm Genome 7 (7), 501-504 (1996)
   PUBMED   8672127
REFERENCE   9  (bases 1 to 5145)
  AUTHORS   Martelli,A.M., Lach,S., Grill,V., Gilmour,R.S., Cocco,L.,
            Narducci,P. and Bareggi,R.
  TITLE     Expression and immunohistochemical localization of eight
            phospholipase C isoforms in adult male mouse cerebellar cortex
  JOURNAL   Acta Histochem 98 (2), 131-141 (1996)
   PUBMED   8739298
REFERENCE   10 (bases 1 to 5145)
  AUTHORS   Hempel,W.M. and DeFranco,A.L.
  TITLE     Expression of phospholipase C isozymes by murine B lymphocytes
  JOURNAL   J Immunol 146 (11), 3713-3720 (1991)
   PUBMED   2033248
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB635904.1, AK045469.1, BB620972.1, AL772255.12 and AK046511.1.
            
            On Mar 22, 2005 this sequence version replaced NM_177568.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a). The exon combination
            of this variant is inferred based on partial mouse transcript
            alignments and full-length orthologous support.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR13422601.623321.1,
                                           SRR13422600.1488179.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-535               BB635904.1         2-536
            536-2181            AK045469.1         421-2066
            2182-2484           BB620972.1         269-571
            2485-2551           AL772255.12        7611-7677           c
            2552-2646           AL772255.12        7373-7467           c
            2647-2719           AL772255.12        7175-7247           c
            2720-2767           AL772255.12        6926-6973           c
            2768-2978           AL772255.12        6580-6790           c
            2979-3141           AK046511.1         117-279
            3142-3218           AK046511.1         375-451
            3219-3314           AK046511.1         550-645
            3315-3979           AK046511.1         1112-1776
            3980-5145           AL772255.12        2761-3926           c
FEATURES             Location/Qualifiers
     source          1..5145
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 59.43 cM"
     gene            1..5145
                     /gene="Plcb2"
                     /gene_synonym="B230205M18Rik; B230399N12"
                     /note="phospholipase C, beta 2"
                     /db_xref="GeneID:18796"
                     /db_xref="MGI:MGI:107465"
     exon            1..201
                     /gene="Plcb2"
                     /gene_synonym="B230205M18Rik; B230399N12"
                     /inference="alignment:Splign:2.1.0"
     CDS             118..3663
                     /gene="Plcb2"
                     /gene_synonym="B230205M18Rik; B230399N12"
                     /EC_number="3.1.4.11"
                     /note="isoform a is encoded by transcript variant 1;
                     PLC-beta-2; phosphoinositide phospholipase C-beta-2;
                     1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
                     beta-2"
                     /codon_start=1
                     /product="1-phosphatidylinositol 4,5-bisphosphate
                     phosphodiesterase beta-2 isoform a"
                     /protein_id="NP_808236.2"
                     /db_xref="CCDS:CCDS16582.1"
                     /db_xref="GeneID:18796"
                     /db_xref="MGI:MGI:107465"
                     /translation="MSLLNPVLLPPNVKAYLSQGERFIKWDDETSIASPVILRVDPKG
                     YYLYWTYQNQEMEFLDVTSIRDTRFGKFAKIPKSQKLREVFNMDFPDNHFLLKTLTVV
                     SGPDMVDLTFYNFVSYKENVGKDWAEDVLALAKHPMTVNAPRSTFLDKILVKLKMQLN
                     PEGKIPVKNFFQMFPADRKRVEAALGACHLAKGKNDAINPEDFPESVYKSFLMSLCPR
                     PEIDEIFTSYHSKAKPYMTKEHLTKFINQKQRDPRLNSLLFPPARPEQVQVLIDKYEP
                     SGINVQRGQLSPEGMVWFLCGPENSVLAHDTLLIHQDMTQPLNHYFINSSHNTYLTAG
                     QFSGLSSAEMYRQVLLSGCRCVELDCWKGKPPDEEPIITHGFTMTTDILFKEAIEAIA
                     ESAFKTSPYPVILSFENHVDSPRQQAKMAEYCRSMFGETLLTDPLENFPLKPGIPLPS
                     PEDLRGKILIKNKKNQFSGPASPSKKPGGVAEGSLPSSVPVEEDTGWTAEDRTEVEEE
                     EVVEEEEEEESGNLDEEEIKKMQSDEGTAGLEVTAYEEMSSLVNYIQPTKFISFEFSA
                     QKNRSYVVSSFTELKAYELLSKASMQFVDYNKRQMSRVYPKGTRMDSSNYMPQMFWNA
                     GCQMVALNFQTMDLPMQQNMALFEFNGQSGYLLKHEFMRRLDKQFNPFSVDRIDVVVA
                     TTLSITIISGQFLSERSVRTYVEVELFGLPGDPKRRYRTKLSPTANSINPVWKEEPFI
                     FEKILMPELASLRIAVMEEGSKFLGHRIIPINALHSGYHHLCLRSESNMALTMPALFV
                     FLEMKDYIPDTWADLTVALANPIKYFNAQDKKSVKLKGVTGSLPEKLFSGTPVASQSN
                     GAPVSAGNGSTAPGTKATGEEATKEVTEPQTASLEELRELKGVVKLQRRHEKELRELE
                     RRGARRWEELLQRGAAQLAELQTQAAGCKLRPGKGSRKKRTLPCEETVVAPSEPHDRA
                     DPRVQELKDRLEQELQQQGEEQYRSVLKRKEQHVTEQIAKMMELAREKQAAELKTFKE
                     TSETDTKEMKKKLEAKRLERIQAMTKVTTDKVAQERLKREINNSHIQEVVQAVKQMTE
                     TLERHQEKLEERQTACLEQIQAMEKQFQEKALAEYEAKMKGLEAEVKESVRAYFKDCF
                     PTEAEDKPERSCEASEESCPQEPLVSKADTQESRL"
     exon            202..279
                     /gene="Plcb2"
                     /gene_synonym="B230205M18Rik; B230399N12"
                     /inference="alignment:Splign:2.1.0"
     exon            280..348
                     /gene="Plcb2"
                     /gene_synonym="B230205M18Rik; B230399N12"
                     /inference="alignment:Splign:2.1.0"
     exon            349..489
                     /gene="Plcb2"
                     /gene_synonym="B230205M18Rik; B230399N12"
                     /inference="alignment:Splign:2.1.0"
     exon            490..569
                     /gene="Plcb2"
                     /gene_synonym="B230205M18Rik; B230399N12"
                     /inference="alignment:Splign:2.1.0"
     exon            570..623
                     /gene="Plcb2"
                     /gene_synonym="B230205M18Rik; B230399N12"
                     /inference="alignment:Splign:2.1.0"
     exon            624..699
                     /gene="Plcb2"
                     /gene_synonym="B230205M18Rik; B230399N12"
                     /inference="alignment:Splign:2.1.0"
     exon            700..800
                     /gene="Plcb2"
                     /gene_synonym="B230205M18Rik; B230399N12"
                     /inference="alignment:Splign:2.1.0"
     exon            801..967
                     /gene="Plcb2"
                     /gene_synonym="B230205M18Rik; B230399N12"
                     /inference="alignment:Splign:2.1.0"
     exon            968..1114
                     /gene="Plcb2"
                     /gene_synonym="B230205M18Rik; B230399N12"
                     /inference="alignment:Splign:2.1.0"
     exon            1115..1272
                     /gene="Plcb2"
                     /gene_synonym="B230205M18Rik; B230399N12"
                     /inference="alignment:Splign:2.1.0"
     exon            1273..1355
                     /gene="Plcb2"
                     /gene_synonym="B230205M18Rik; B230399N12"
                     /inference="alignment:Splign:2.1.0"
     exon            1356..1440
                     /gene="Plcb2"
                     /gene_synonym="B230205M18Rik; B230399N12"
                     /inference="alignment:Splign:2.1.0"
     exon            1441..1603
                     /gene="Plcb2"
                     /gene_synonym="B230205M18Rik; B230399N12"
                     /inference="alignment:Splign:2.1.0"
     exon            1604..1719
                     /gene="Plcb2"
                     /gene_synonym="B230205M18Rik; B230399N12"
                     /inference="alignment:Splign:2.1.0"
     exon            1720..1816
                     /gene="Plcb2"
                     /gene_synonym="B230205M18Rik; B230399N12"
                     /inference="alignment:Splign:2.1.0"
     exon            1817..1901
                     /gene="Plcb2"
                     /gene_synonym="B230205M18Rik; B230399N12"
                     /inference="alignment:Splign:2.1.0"
     exon            1902..2026
                     /gene="Plcb2"
                     /gene_synonym="B230205M18Rik; B230399N12"
                     /inference="alignment:Splign:2.1.0"
     exon            2027..2181
                     /gene="Plcb2"
                     /gene_synonym="B230205M18Rik; B230399N12"
                     /inference="alignment:Splign:2.1.0"
     exon            2182..2346
                     /gene="Plcb2"
                     /gene_synonym="B230205M18Rik; B230399N12"
                     /inference="alignment:Splign:2.1.0"
     exon            2347..2446
                     /gene="Plcb2"
                     /gene_synonym="B230205M18Rik; B230399N12"
                     /inference="alignment:Splign:2.1.0"
     exon            2447..2551
                     /gene="Plcb2"
                     /gene_synonym="B230205M18Rik; B230399N12"
                     /inference="alignment:Splign:2.1.0"
     exon            2552..2646
                     /gene="Plcb2"
                     /gene_synonym="B230205M18Rik; B230399N12"
                     /inference="alignment:Splign:2.1.0"
     exon            2647..2719
                     /gene="Plcb2"
                     /gene_synonym="B230205M18Rik; B230399N12"
                     /inference="alignment:Splign:2.1.0"
     exon            2720..2767
                     /gene="Plcb2"
                     /gene_synonym="B230205M18Rik; B230399N12"
                     /inference="alignment:Splign:2.1.0"
     exon            2768..2978
                     /gene="Plcb2"
                     /gene_synonym="B230205M18Rik; B230399N12"
                     /inference="alignment:Splign:2.1.0"
     exon            2979..3141
                     /gene="Plcb2"
                     /gene_synonym="B230205M18Rik; B230399N12"
                     /inference="alignment:Splign:2.1.0"
     exon            3142..3218
                     /gene="Plcb2"
                     /gene_synonym="B230205M18Rik; B230399N12"
                     /inference="alignment:Splign:2.1.0"
     exon            3219..3314
                     /gene="Plcb2"
                     /gene_synonym="B230205M18Rik; B230399N12"
                     /inference="alignment:Splign:2.1.0"
     exon            3315..3372
                     /gene="Plcb2"
                     /gene_synonym="B230205M18Rik; B230399N12"
                     /inference="alignment:Splign:2.1.0"
     exon            3373..3459
                     /gene="Plcb2"
                     /gene_synonym="B230205M18Rik; B230399N12"
                     /inference="alignment:Splign:2.1.0"
     exon            3460..5145
                     /gene="Plcb2"
                     /gene_synonym="B230205M18Rik; B230399N12"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      3790
                     /gene="Plcb2"
                     /gene_synonym="B230205M18Rik; B230399N12"
                     /note="major polyA site"
     polyA_site      3896
                     /gene="Plcb2"
                     /gene_synonym="B230205M18Rik; B230399N12"
                     /note="major polyA site"
     polyA_site      4722
                     /gene="Plcb2"
                     /gene_synonym="B230205M18Rik; B230399N12"
     regulatory      4896..4901
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Plcb2"
                     /gene_synonym="B230205M18Rik; B230399N12"
                     /note="hexamer: AATAAA"
     polyA_site      4916
                     /gene="Plcb2"
                     /gene_synonym="B230205M18Rik; B230399N12"
                     /note="major polyA site"
     regulatory      5116..5121
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Plcb2"
                     /gene_synonym="B230205M18Rik; B230399N12"
                     /note="hexamer: AGTAAA"
     polyA_site      5145
                     /gene="Plcb2"
                     /gene_synonym="B230205M18Rik; B230399N12"
ORIGIN      
        1 gggcgggtgc catgtgagcc tagcctaagc ttcctctcct gttcaccctt cttccttctc
       61 ttctcctgtc ccctggagag gtttgcccaa cctctccaaa gagggacact tgacatcatg
      121 tctttgctca accctgttct attgccccct aacgtgaagg catatctgag ccaaggggag
      181 cgcttcatca agtgggacga tgaaacttca atagcctctc ctgttatcct ccgtgtggat
      241 cccaagggct attacttata ctggacatat caaaatcagg agatggagtt cctggatgtc
      301 acgagtatcc gagatactcg ctttgggaag tttgcaaaga tacccaagag ccagaaactc
      361 cgggaggtct tcaacatgga ctttccagac aaccacttcc tgctgaaaac actcacagtg
      421 gtgtccggcc ctgacatggt ggacctcacc ttctacaact ttgtctctta caaggagaac
      481 gtaggcaagg actgggctga ggacgtgcta gctctcgcca aacacccgat gacagtcaat
      541 gctcctcgaa gcacattcct ggacaagatc ctggtgaagc ttaagatgca gctcaaccct
      601 gaagggaaga ttcccgtgaa gaattttttc cagatgtttc ctgctgatcg aaaacgggtg
      661 gaagctgccc tcggtgcttg tcaccttgca aaaggcaaaa atgatgctat caacccagag
      721 gacttcccag aatccgtgta caagagcttc ctcatgagcc tctgtcctcg gccagaaatc
      781 gacgagatct tcacttccta ccactctaaa gctaagccct acatgaccaa ggaacacctg
      841 accaaattca tcaatcagaa gcagcgagac cctcgactca actccttgct gttcccacca
      901 gcccggcctg agcaagttca agtgctcatt gacaagtacg aacccagcgg catcaatgtg
      961 cagaggggcc agctgtcacc agaggggatg gtctggtttc tctgtggacc agagaacagt
     1021 gtgctggccc acgatacact gctgatccac caagacatga cacaaccact gaatcactat
     1081 ttcatcaact cctcacacaa cacctacctg acagccggcc agttctcagg cctttcctcg
     1141 gctgagatgt accgccaggt gctgctgtct ggctgccggt gtgtagagtt agactgttgg
     1201 aagggcaagc cccctgacga agagcccatt atcacccacg gcttcaccat gaccacagat
     1261 atcttgttca aggaagcaat tgaagccatt gcagaaagtg cctttaagac ctccccgtat
     1321 cctgtcatcc tatcatttga aaaccatgta gactcacccc ggcaacaggc taagatggct
     1381 gagtactgca ggagcatgtt tggagagacc ttgctcacag atcccctgga aaatttccct
     1441 ctgaaacctg gcatccctct gcccagcccc gaggacctcc ggggcaagat cctcattaag
     1501 aataagaaga accagttttc tgggccagca tcccccagca agaagcctgg tggggtggct
     1561 gagggcagcc tcccgtctag tgtccctgta gaagaggaca cggggtggac tgctgaggac
     1621 cggactgagg tggaggagga ggaggtggtg gaagaggagg aagaggagga gtcaggaaac
     1681 ctggatgaag aagaaattaa gaagatgcag tcggatgagg gcacagcagg cctggaggtg
     1741 acagcttatg aggaaatgtc cagccttgtc aattatatcc agcccaccaa gtttatctcc
     1801 ttcgagttct ctgcacagaa gaacagaagt tacgttgtct cttccttcac ggagctcaag
     1861 gcctacgagc tgctctccaa ggcctccatg cagtttgtgg actataataa acgccagatg
     1921 agccgtgtgt accccaaggg cacacgcatg gactcttcca actacatgcc ccagatgttc
     1981 tggaatgctg gatgtcagat ggttgccctc aatttccaga caatggacct gcccatgcag
     2041 cagaacatgg cactgtttga gttcaacggg cagagtggct acctcctaaa gcacgagttc
     2101 atgcgcagac tggacaagca gttcaacccc ttctcagtgg accgcattga tgtggtggta
     2161 gccaccaccc tttccattac gatcatctct gggcagttcc tgtcagagcg cagtgtacgc
     2221 acctatgtgg aagtggaact gtttggcctc ccaggggacc ccaagaggcg gtatcgaacc
     2281 aagctgtcac ctactgctaa ctccatcaat cctgtctgga aagaggaacc ctttatcttt
     2341 gagaagatct tgatgcctga gctggcctcc ctcaggatag ctgtgatgga agaaggcagc
     2401 aaatttcttg gacaccgcat catccccatc aatgccttac attctggata ccaccatctg
     2461 tgcctgcgca gtgagagcaa catggccctt accatgccag ctctctttgt cttcctggag
     2521 atgaaggact acatacctga cacctgggca gatcttacag tagcccttgc caaccccatc
     2581 aagtacttca atgcccagga taagaagtcc gtgaagctca aaggagtgac aggaagtctg
     2641 cccgagaaac tcttctctgg gacacctgtt gccagtcagt ccaatggggc gccagtctcg
     2701 gcaggcaatg ggtcaacagc gcctgggacc aaggccacgg gggaagaagc tacgaaagaa
     2761 gtgacagagc cacagaccgc cagcttggaa gagttgcggg aactgaaggg cgtggtgaaa
     2821 ctgcagcgaa gacatgagaa ggagcttcga gagttggagc gccgtggagc ccggcgctgg
     2881 gaggaactgc tgcagcgtgg cgccgcacag ctggcggagc tccagaccca ggcagcaggc
     2941 tgcaagctcc gcccaggcaa gggctcccgc aagaagagga ccctgccctg tgaggagacc
     3001 gtcgtggcgc ctagcgagcc ccacgaccgg gcggacccgc gcgtgcagga gctgaaggac
     3061 aggctggagc aggagctgca gcagcaaggc gaggagcagt accgctccgt cctcaagcgc
     3121 aaggagcagc acgtgaccga gcaaatcgcc aagatgatgg agctggccag agagaaacag
     3181 gctgctgaac tcaagacctt caaggagacc tcagaaactg acaccaaaga aatgaagaaa
     3241 aaactggagg ccaagaggct ggaacggatc caagccatga ccaaggtcac cacagacaag
     3301 gtggcccagg agaggctcaa gagagaaatt aacaactccc acatccagga agtggtccag
     3361 gctgtcaagc agatgacaga gaccctggag cgtcaccagg agaagctgga agagaggcag
     3421 acagcctgcc tggagcagat ccaggcaatg gaaaagcagt tccaggagaa ggcgctggca
     3481 gagtatgagg ccaagatgaa gggcctggag gctgaagtaa aggagtcggt gcgggcctac
     3541 ttcaaggact gcttccccac cgaagcagaa gacaagcctg agaggtcctg tgaggcctct
     3601 gaggagtcgt gtccacagga accacttgtg agcaaggcag acactcagga gagccgcctc
     3661 tgatgccagc atctcgcttc gacatttaag caaggatgtc cacactcttc tctggaacat
     3721 ggtcccattt cccaggagaa agggacctta atactcagat aaggcaggga ttagagacta
     3781 tcaagggcaa gggtcctcat ggaacctggg tcttttctaa gctggcttct tctgtctgtc
     3841 ccacctgtag tgccattgaa ggcaaaggaa tatggaccaa gagagaggaa actgggtcct
     3901 ggcctcccct ctgcctttcc taatgtacaa acgggcgctc cacttggagc tccgttccct
     3961 ttcctggaca cttggctttg ctccctggaa ccttccctct tgtgggcgtg gctccagcag
     4021 aaccaagtcc tcctgccttg atgcatgcct tgaagcagta ccttacacag gcttctaggc
     4081 tgcatcttgg gcctgccttg agtacagagc atttgctaat gccttaaggc agagcctgat
     4141 actgcctcaa agctctctgg gaatggctac ttccagaatg gcagccaagg gccctgccct
     4201 cacctcctgc ctatggagat agatggcttc ctgggaaagc tttctcagct gcccctcaat
     4261 tgatcaagta ttgggggttt gggggctaca cctgctggca aagggagact agcttctaga
     4321 gtgctctctc caactctgaa actggttcac cctcttttct ggatctaggt ctggcacttc
     4381 ctggcaggtg cgccaccagt cttgggtggg ggcttgagtt catcgtcatt ttcttcactg
     4441 aatttattta tttttctcct tcctaccaaa gaacccaatc ccagaatccc ctgccttagc
     4501 ctggcctctg ttctccagtt aggaaaagac agggggagtg gaaggcctct gaagccaagc
     4561 tgcgtgagtg gagtgtgggg aatggatccc ttgggggcta cagaaagagt gaaggctctt
     4621 ctggggccct gtcaagtggc cctgcctctg gcttccctgt ctgctggccc tgatgccaga
     4681 atccttaaaa gatgatgtta ttctcgcttc tctacacccc cacagatatg ttccttcctt
     4741 gtctctcctg accagggctg gagcagaaga tcccagtaca aaagtgcacc caccctcttg
     4801 gtaaccccaa acctttgagc cgtctgggcc tctttaggaa caccgagagt gaccacacac
     4861 acacacagag acctgcccct gctgtggttg gatccaataa actttctgga ggcatagctt
     4921 gtgggccaca ccccccagct ccgtctcaaa tcccaaatgg gccaacaggg cagggtgatg
     4981 ctgggaagag gttgttttct tatcagagag ttacaacaga tgccagtaac tgtgagcacc
     5041 tgtgtctttg gaaaatgaat agggacgccc tccctatccc tcccagggcc tgacctcaca
     5101 ggtccctggc cacagagtaa agctaagacc tgttcattca tacaa
//
