LOCUS       NM_001145830            6998 bp    mRNA    linear   ROD 06-AUG-2024
DEFINITION  Mus musculus phospholipase C, beta 1 (Plcb1), transcript variant 1,
            mRNA.
ACCESSION   NM_001145830
VERSION     NM_001145830.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6998)
  AUTHORS   Lee,J., Jeong,Y., Park,S., Kim,S., Oh,H., Jin,J.A., Sohn,J.W.,
            Kim,D., Shin,H.S. and Heo,W.D.
  TITLE     Phospholipase C beta 1 in the dentate gyrus gates fear memory
            formation through regulation of neuronal excitability
  JOURNAL   Sci Adv 10 (27), eadj4433 (2024)
   PUBMED   38959322
  REMARK    GeneRIF: Phospholipase C beta 1 in the dentate gyrus gates fear
            memory formation through regulation of neuronal excitability.
REFERENCE   2  (bases 1 to 6998)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 6998)
  AUTHORS   Qiu,C., Martin,B.K., Welsh,I.C., Daza,R.M., Le,T.M., Huang,X.,
            Nichols,E.K., Taylor,M.L., Fulton,O., O'Day,D.R., Gomes,A.R.,
            Ilcisin,S., Srivatsan,S., Deng,X., Disteche,C.M., Noble,W.S.,
            Hamazaki,N., Moens,C.B., Kimelman,D., Cao,J., Schier,A.F.,
            Spielmann,M., Murray,S.A., Trapnell,C. and Shendure,J.
  TITLE     A single-cell time-lapse of mouse prenatal development from
            gastrula to birth
  JOURNAL   Nature 626 (8001), 1084-1093 (2024)
   PUBMED   38355799
REFERENCE   4  (bases 1 to 6998)
  AUTHORS   Feldthouse,M.G., Vyleta,N.P. and Smith,S.M.
  TITLE     PLC regulates spontaneous glutamate release triggered by
            extracellular calcium and readily releasable pool size in
            neocortical neurons
  JOURNAL   Front Cell Neurosci 17, 1193485 (2023)
   PUBMED   37260580
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6998)
  AUTHORS   Sheppard,S.E., Bryant,L., Wickramasekara,R.N., Vaccaro,C.,
            Robertson,B., Hallgren,J., Hulen,J., Watson,C.J., Faundes,V.,
            Duffourd,Y., Lee,P., Simon,M.C., de la Cruz,X., Padilla,N.,
            Flores-Mendez,M., Akizu,N., Smiler,J., Pellegrino Da Silva,R.,
            Li,D., March,M., Diaz-Rosado,A., Peixoto de Barcelos,I., Choa,Z.X.,
            Lim,C.Y., Dubourg,C., Journel,H., Demurger,F., Mulhern,M.,
            Akman,C., Lippa,N., Andrews,M., Baldridge,D., Constantino,J., van
            Haeringen,A., Snoeck-Streef,I., Chow,P., Hing,A., Graham,J.M. Jr.,
            Au,M., Faivre,L., Shen,W., Mao,R., Palumbos,J., Viskochil,D.,
            Gahl,W., Tifft,C., Macnamara,E., Hauser,N., Miller,R., Maffeo,J.,
            Afenjar,A., Doummar,D., Keren,B., Arn,P., Macklin-Mantia,S.,
            Meerschaut,I., Callewaert,B., Reis,A., Zweier,C., Brewer,C.,
            Saggar,A., Smeland,M.F., Kumar,A., Elmslie,F., Deshpande,C.,
            Nizon,M., Cogne,B., van Ierland,Y., Wilke,M., van Slegtenhorst,M.,
            Koudijs,S., Chen,J.Y., Dredge,D., Pier,D., Wortmann,S.,
            Kamsteeg,E.J., Koch,J., Haynes,D., Pollack,L., Titheradge,H.,
            Ranguin,K., Denomme-Pichon,A.S., Weber,S., Perez de la Fuente,R.,
            Sanchez Del Pozo,J., Lezana Rosales,J.M., Joset,P., Steindl,K.,
            Rauch,A., Mei,D., Mari,F., Guerrini,R., Lespinasse,J., Tran
            Mau-Them,F., Philippe,C., Dauriat,B., Raymond,L., Moutton,S.,
            Cueto-Gonzalez,A.M., Tan,T.Y., Mignot,C., Grotto,S., Renaldo,F.,
            Drivas,T.G., Hennessy,L., Raper,A., Parenti,I., Kaiser,F.J.,
            Kuechler,A., Busk,O.L., Islam,L., Siedlik,J.A., Henderson,L.B.,
            Juusola,J., Person,R., Schnur,R.E., Vitobello,A., Banka,S.,
            Bhoj,E.J. and Stessman,H.A.F.
  TITLE     Mechanism of KMT5B haploinsufficiency in neurodevelopment in humans
            and mice
  JOURNAL   Sci Adv 9 (10), eade1463 (2023)
   PUBMED   36897941
REFERENCE   6  (bases 1 to 6998)
  AUTHORS   Lyu,M.S., Park,D.J., Rhee,S.G. and Kozak,C.A.
  TITLE     Genetic mapping of the human and mouse phospholipase C genes
  JOURNAL   Mamm Genome 7 (7), 501-504 (1996)
   PUBMED   8672127
REFERENCE   7  (bases 1 to 6998)
  AUTHORS   Zuberi,A.R., Nguyen,H.Q., Auman,H.J., Taylor,B.A. and
            Roopenian,D.C.
  TITLE     A genetic linkage map of mouse chromosome 2 extending from
            thrombospondin to paired box gene 1, including the H3 minor
            histocompatibility complex
  JOURNAL   Genomics 33 (1), 75-84 (1996)
   PUBMED   8617512
REFERENCE   8  (bases 1 to 6998)
  AUTHORS   Walensky,L.D. and Snyder,S.H.
  TITLE     Inositol 1,4,5-trisphosphate receptors selectively localized to the
            acrosomes of mammalian sperm
  JOURNAL   J Cell Biol 130 (4), 857-869 (1995)
   PUBMED   7642703
REFERENCE   9  (bases 1 to 6998)
  AUTHORS   Hess,E.J., Collins,K.A., Copeland,N.G., Jenkins,N.A. and
            Wilson,M.C.
  TITLE     Deletion map of the coloboma (Cm) locus on mouse chromosome 2
  JOURNAL   Genomics 21 (1), 257-261 (1994)
   PUBMED   7916325
REFERENCE   10 (bases 1 to 6998)
  AUTHORS   Hempel,W.M. and DeFranco,A.L.
  TITLE     Expression of phospholipase C isozymes by murine B lymphocytes
  JOURNAL   J Immunol 146 (11), 3713-3720 (1991)
   PUBMED   2033248
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK143153.1, BC058710.1 and AL935278.7.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC058710.1, AK141164.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849385
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-33                AK143153.1         2-34
            34-4659             BC058710.1         1-4626
            4660-6998           AL935278.7         158834-161172
FEATURES             Location/Qualifiers
     source          1..6998
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 65.66 cM"
     gene            1..6998
                     /gene="Plcb1"
                     /gene_synonym="3110043I21Rik; mKIAA0581; Plcb"
                     /note="phospholipase C, beta 1"
                     /db_xref="GeneID:18795"
                     /db_xref="MGI:MGI:97613"
     exon            1..468
                     /gene="Plcb1"
                     /gene_synonym="3110043I21Rik; mKIAA0581; Plcb"
                     /inference="alignment:Splign:2.1.0"
     CDS             370..4020
                     /gene="Plcb1"
                     /gene_synonym="3110043I21Rik; mKIAA0581; Plcb"
                     /EC_number="3.1.4.11"
                     /note="isoform 1 is encoded by transcript variant 1;
                     phospholipase C-beta-1a; phosphoinositide phospholipase C;
                     PLC-154; PLC-beta-1; phosphoinositide phospholipase
                     C-beta-1; 1-phosphatidylinositol 4,5-bisphosphate
                     phosphodiesterase beta-1"
                     /codon_start=1
                     /product="1-phosphatidylinositol 4,5-bisphosphate
                     phosphodiesterase beta-1 isoform 1"
                     /protein_id="NP_001139302.1"
                     /db_xref="CCDS:CCDS50729.1"
                     /db_xref="GeneID:18795"
                     /db_xref="MGI:MGI:97613"
                     /translation="MAGAQPGVHALQLKPVCVSDSLKKGTKFVKWDDDSTIVTPIILR
                     TDPQGFFFYWTDQNKETELLDLSLVKDARCGKHAKAPKDPKLRELLDVGNIGHLEQRM
                     ITVVYGPDLVNISHLNLVAFQEEVAKEWTNEVFSLATNLLAQNMSRDAFLEKAYTKLK
                     LQVTPEGRIPLKNIYRLFSADRKRVETALEACSLPSSRNDSIPQEDFTPDVYRVFLNN
                     LCPRPEIDNIFSEFGAKSKPYLTVDQMMDFINLKQRDPRLNEILYPPLKQEQVQVLIE
                     KYEPNSSLAKKGQMSVDGFMRYLSGEENGVVSPEKLDLNEDMSQPLSHYFINSSHNTY
                     LTAGQLAGNSSVEMYRQVLLSGCRCVELDCWKGRTAEEEPVITHGFTMTTEISFKEVI
                     EAIAECAFKTSPFPILLSFENHVDSPKQQAKMAEYCRLIFGDALLMEPLEKYPLESGV
                     PLPSPMDLMYKILVKNKKKSHKSSEGSGKKKLSEQASNTYSDSSSVFEPSSPGAGEAD
                     TESDDDDDDDDCKKSSMDEGTAGSEAMATEEMSNLVNYIQPVKFESFEISKKRNKSFE
                     MSSFVETKGLEQLTKSPVEFVEYNKMQLSRIYPKGTRVDSSNYMPQLFWNAGCQMVAL
                     NFQTVDLAMQINMGMYEYNGKSGYRLKPEFMRRPDKHFDPFTEGIVDGIVANTLSVKI
                     ISGQFLSDKKVGTYVEVDMFGLPVDTRRKAFKTKTSQGNAVNPVWEEEPIVFKKVVLP
                     SLACLRIAAYEEGGKFIGHRILPVQAIRPGYHYICLRNERNQPLTLPAVFVYIEVKDY
                     VPDTYADVIEALSNPIRYVNLMEQRAKQLAALTLEDEEEVKKEADPGETSSEAPSETR
                     TTPAENGVNHTASLAPKPPSQAPHSQPAPGSVKAPAKTEDLIQSVLTEVEAQTIEELK
                     QQKSFVKLQKKHYKEMKDLVKRHHKKTTELIKEHTTKYNEIQNDYLRRRAALEKSAKK
                     DSKKKSEPSSPDHGSSAIEQDLAALDAEMTQKLIDLKDKQQQQLLNLRQEQYYSEKYQ
                     KREHIKLLIQKLTDVAEECQNNQLKKLKEICEKEKKELKKKMDKKRQEKITEAKSKDK
                     SQMEEEKTEMIRSYIQEVVQYIKRLEEAQSKRQEKLVEKHNEIRQQILDEKPKLQTEL
                     EQEYQDKFKRLPLEILEFVQEAMKGKISEDSNHGSAPPSLASDAAKVNLKSPSSEEIE
                     RENPGREFDTPL"
     exon            469..546
                     /gene="Plcb1"
                     /gene_synonym="3110043I21Rik; mKIAA0581; Plcb"
                     /inference="alignment:Splign:2.1.0"
     exon            547..615
                     /gene="Plcb1"
                     /gene_synonym="3110043I21Rik; mKIAA0581; Plcb"
                     /inference="alignment:Splign:2.1.0"
     exon            616..753
                     /gene="Plcb1"
                     /gene_synonym="3110043I21Rik; mKIAA0581; Plcb"
                     /inference="alignment:Splign:2.1.0"
     exon            754..833
                     /gene="Plcb1"
                     /gene_synonym="3110043I21Rik; mKIAA0581; Plcb"
                     /inference="alignment:Splign:2.1.0"
     exon            834..887
                     /gene="Plcb1"
                     /gene_synonym="3110043I21Rik; mKIAA0581; Plcb"
                     /inference="alignment:Splign:2.1.0"
     exon            888..963
                     /gene="Plcb1"
                     /gene_synonym="3110043I21Rik; mKIAA0581; Plcb"
                     /inference="alignment:Splign:2.1.0"
     exon            964..1064
                     /gene="Plcb1"
                     /gene_synonym="3110043I21Rik; mKIAA0581; Plcb"
                     /inference="alignment:Splign:2.1.0"
     exon            1065..1231
                     /gene="Plcb1"
                     /gene_synonym="3110043I21Rik; mKIAA0581; Plcb"
                     /inference="alignment:Splign:2.1.0"
     exon            1232..1378
                     /gene="Plcb1"
                     /gene_synonym="3110043I21Rik; mKIAA0581; Plcb"
                     /inference="alignment:Splign:2.1.0"
     exon            1379..1536
                     /gene="Plcb1"
                     /gene_synonym="3110043I21Rik; mKIAA0581; Plcb"
                     /inference="alignment:Splign:2.1.0"
     exon            1537..1619
                     /gene="Plcb1"
                     /gene_synonym="3110043I21Rik; mKIAA0581; Plcb"
                     /inference="alignment:Splign:2.1.0"
     exon            1620..1704
                     /gene="Plcb1"
                     /gene_synonym="3110043I21Rik; mKIAA0581; Plcb"
                     /inference="alignment:Splign:2.1.0"
     exon            1705..1882
                     /gene="Plcb1"
                     /gene_synonym="3110043I21Rik; mKIAA0581; Plcb"
                     /inference="alignment:Splign:2.1.0"
     exon            1883..1950
                     /gene="Plcb1"
                     /gene_synonym="3110043I21Rik; mKIAA0581; Plcb"
                     /inference="alignment:Splign:2.1.0"
     exon            1951..2047
                     /gene="Plcb1"
                     /gene_synonym="3110043I21Rik; mKIAA0581; Plcb"
                     /inference="alignment:Splign:2.1.0"
     exon            2048..2132
                     /gene="Plcb1"
                     /gene_synonym="3110043I21Rik; mKIAA0581; Plcb"
                     /inference="alignment:Splign:2.1.0"
     exon            2133..2257
                     /gene="Plcb1"
                     /gene_synonym="3110043I21Rik; mKIAA0581; Plcb"
                     /inference="alignment:Splign:2.1.0"
     exon            2258..2412
                     /gene="Plcb1"
                     /gene_synonym="3110043I21Rik; mKIAA0581; Plcb"
                     /inference="alignment:Splign:2.1.0"
     exon            2413..2577
                     /gene="Plcb1"
                     /gene_synonym="3110043I21Rik; mKIAA0581; Plcb"
                     /inference="alignment:Splign:2.1.0"
     exon            2578..2677
                     /gene="Plcb1"
                     /gene_synonym="3110043I21Rik; mKIAA0581; Plcb"
                     /inference="alignment:Splign:2.1.0"
     exon            2678..2782
                     /gene="Plcb1"
                     /gene_synonym="3110043I21Rik; mKIAA0581; Plcb"
                     /inference="alignment:Splign:2.1.0"
     exon            2783..2892
                     /gene="Plcb1"
                     /gene_synonym="3110043I21Rik; mKIAA0581; Plcb"
                     /inference="alignment:Splign:2.1.0"
     exon            2893..3025
                     /gene="Plcb1"
                     /gene_synonym="3110043I21Rik; mKIAA0581; Plcb"
                     /inference="alignment:Splign:2.1.0"
     exon            3026..3079
                     /gene="Plcb1"
                     /gene_synonym="3110043I21Rik; mKIAA0581; Plcb"
                     /inference="alignment:Splign:2.1.0"
     exon            3080..3299
                     /gene="Plcb1"
                     /gene_synonym="3110043I21Rik; mKIAA0581; Plcb"
                     /inference="alignment:Splign:2.1.0"
     exon            3300..3480
                     /gene="Plcb1"
                     /gene_synonym="3110043I21Rik; mKIAA0581; Plcb"
                     /inference="alignment:Splign:2.1.0"
     exon            3481..3557
                     /gene="Plcb1"
                     /gene_synonym="3110043I21Rik; mKIAA0581; Plcb"
                     /inference="alignment:Splign:2.1.0"
     exon            3558..3647
                     /gene="Plcb1"
                     /gene_synonym="3110043I21Rik; mKIAA0581; Plcb"
                     /inference="alignment:Splign:2.1.0"
     exon            3648..3705
                     /gene="Plcb1"
                     /gene_synonym="3110043I21Rik; mKIAA0581; Plcb"
                     /inference="alignment:Splign:2.1.0"
     exon            3706..3792
                     /gene="Plcb1"
                     /gene_synonym="3110043I21Rik; mKIAA0581; Plcb"
                     /inference="alignment:Splign:2.1.0"
     exon            3793..6998
                     /gene="Plcb1"
                     /gene_synonym="3110043I21Rik; mKIAA0581; Plcb"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6978..6983
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Plcb1"
                     /gene_synonym="3110043I21Rik; mKIAA0581; Plcb"
                     /note="hexamer: AATAAA"
     polyA_site      6997
                     /gene="Plcb1"
                     /gene_synonym="3110043I21Rik; mKIAA0581; Plcb"
                     /note="major polyA site"
ORIGIN      
        1 actcgcgcgc tccctctgtg cacagaggat gtgctgaatg gtgcgcttcc aggcggcggc
       61 cgagcaggat ccagcggcgg gcgactcgca ctgcccggct ctgctcctcc gcgtctcccg
      121 aggctctaca gtcaccgcgg gctccagatc ctgcgtcccg cccggggcat ggcaggctgc
      181 tgtaccctcg cgtgtcgtcc cgcttactaa gcggcgcggg ccagaggtgc ggaggccaag
      241 aggccggagg ccggcgggca gcgaagccca gagcctggtg aatcgagagc caagcccgct
      301 cgcgcccgga gctccgcgtc ccgtctccac tgcgctcgcc cgggccgccc ggagccccga
      361 tgagcccaga tggctggggc tcagcccgga gtgcacgcct tgcaactcaa gcccgtgtgc
      421 gtgtccgaca gcctcaagaa gggcaccaaa ttcgtcaagt gggatgatga ctccactatt
      481 gttactccaa ttattttgag gactgatcct cagggatttt tcttttactg gacagaccag
      541 aataaggaga cggagctgtt agatctcagc cttgtcaagg atgccaggtg tgggaagcac
      601 gccaaagctc ccaaggaccc caagttgcgt gaacttctgg atgttgggaa catcggacac
      661 ttggaacagc gcatgataac tgtggtgtat ggaccagacc tggtgaacat ttcccacttg
      721 aatcttgtgg cttttcaaga ggaagtagcc aaggaatgga caaatgaggt tttcagcttg
      781 gcaacaaacc tgctggctca gaacatgtcc agggacgcat ttctggagaa agcatatact
      841 aagctcaagc tccaggtcac cccagaaggc cgcattcctc ttaaaaacat ctatcgattg
      901 ttctcagcag accggaagcg cgttgaaact gcactagagg cttgtagtct tccatcttcg
      961 aggaatgact ccattcccca agaggacttc actccagatg tatacagagt tttcctgaac
     1021 aatctctgtc cccgacctga aattgataac atcttttctg aatttggtgc caaaagcaaa
     1081 ccgtacctga cggttgatca gatgatggat tttatcaacc ttaagcagag agatccccgg
     1141 ctcaatgaaa tcctttaccc acctctgaag caggagcagg tccaagtgtt gattgagaaa
     1201 tatgagccca acagcagcct cgccaagaaa gggcagatgt cagtggatgg attcatgcgc
     1261 tacctgagcg gagaagaaaa tggagtcgtt tcacctgaga aactggattt gaacgaagac
     1321 atgtctcagc ccctgtctca ctatttcatc aattcctcgc acaacaccta cctcacagct
     1381 ggccagttgg ctgggaactc gtctgtagag atgtatcgcc aggtgcttct gtctggatgt
     1441 cgctgtgtgg agctggactg ctggaaggga aggaccgctg aggaagagcc tgtcattacc
     1501 catggattca ccatgacaac agaaatatcc ttcaaggaag tcatagaagc catcgcagag
     1561 tgtgcgttca agacgtctcc tttccccatc ctcctttcct ttgagaacca cgtggattcc
     1621 ccgaagcaac aagccaagat ggccgagtat tgccgattaa tctttggtga tgccctcctg
     1681 atggaaccac tggaaaaata cccactggaa tctggggtac ctcttccaag ccctatggat
     1741 ttaatgtata aaatcttggt gaaaaacaag aagaagtcgc acaagtcgtc agagggaagt
     1801 ggtaagaaga agctttcaga gcaagcttcc aacacctaca gcgactcctc cagtgtgttc
     1861 gagccttcgt ctccaggagc tggggaagca gatacggaga gtgatgacga tgacgacgac
     1921 gatgactgta aaaagtcttc catggatgag gggacagctg gcagcgaagc catggccaca
     1981 gaagagatgt ctaacctggt gaactatatt cagcctgtca agtttgagtc ctttgaaatt
     2041 tcaaaaaaaa gaaataaaag ctttgaaatg tcttccttcg tggaaaccaa aggacttgaa
     2101 caacttacaa agtctccggt ggaatttgta gaatacaaca agatgcaact tagcaggata
     2161 tatcccaagg gaacacgtgt ggattcatcc aactacatgc ctcaactctt ctggaatgct
     2221 ggctgtcaga tggtggcgct caacttccag acagtggatc tagctatgca gataaacatg
     2281 ggcatgtatg agtacaatgg gaagagcggc tataggctga agccagagtt catgaggagg
     2341 ccggacaagc attttgatcc atttactgaa ggaatcgtag atgggatagt ggccaacact
     2401 ctatctgtta agattatttc aggtcagttc ctctctgata agaaagttgg gacttatgtg
     2461 gaagtggata tgtttggctt gcctgtggac acaaggagga aggcatttaa aaccaagaca
     2521 tcccaaggaa atgctgtaaa ccctgtctgg gaagaagaac ctattgtatt caaaaaggta
     2581 gttctacctt ctctggcctg tttgaggata gcagcatatg aagagggagg caaatttatt
     2641 ggccaccgga tattgcctgt gcaggctatt cggccaggct atcactacat ctgcctgcgg
     2701 aatgagagga accagcccct gacgctgcca gctgtctttg tctacataga agtcaaagat
     2761 tatgtgccag acacgtatgc agatgtaatc gaagctctat caaacccaat ccgatatgtc
     2821 aatctgatgg aacagagagc taagcagctg gctgcgttga ccctggagga tgaagaggaa
     2881 gtaaagaagg aggctgaccc tggagaaaca tcttccgagg ctccaagcga aaccaggaca
     2941 acgccagcag agaatggggt gaatcacacc gcatcccttg cacccaagcc gccttcccag
     3001 gctccacaca gccagcctgc tccagggtct gtgaaggcac ctgccaaaac agaagatctg
     3061 atccagagcg tgttaacaga agtagaggcg caaaccatcg aagagctcaa gcaacagaaa
     3121 tcgttcgtga aacttcaaaa gaaacactac aaagaaatga aagacctggt gaagagacac
     3181 cacaagaaaa ccacggagct cattaaggag cacacgacca agtacaacga gattcagaat
     3241 gactacctga gaaggagggc agccttggag aagtccgcca aaaaggacag caagaagaaa
     3301 tctgaaccca gcagccctga ccacggctca tcagccattg agcaagacct cgctgccctg
     3361 gatgcagaaa tgactcagaa gttgatagac ttgaaagaca agcaacaaca gcagctgctt
     3421 aatcttcggc aagagcagta ttacagcgag aagtaccaaa agcgagaaca cattaaactg
     3481 cttattcaga agttgacaga tgttgcagaa gagtgtcaga acaatcagtt aaagaagctg
     3541 aaggaaatct gcgagaaaga gaagaaggaa ttaaagaaga aaatggataa gaagaggcaa
     3601 gagaagataa cagaagccaa gtccaaagac aaaagccaga tggaagagga gaagacagag
     3661 atgatccgat cgtacatcca ggaggtggtt cagtacatca agaggttaga ggaagcacaa
     3721 agtaaaagac aagaaaaact cgtggagaaa cacaatgaga tccgccagca gatcctcgat
     3781 gagaagccca agctgcagac ggagctggag caagaatacc aagacaagtt caaaaggctg
     3841 cccctggaga ttctggagtt tgtacaggaa gccatgaaag ggaagatcag cgaggacagc
     3901 aatcacggct ctgcccctcc ctctctggcc tcagatgctg caaaggtgaa cctcaagtct
     3961 ccctccagtg aggagataga aagagagaac ccaggaagag agtttgatac tcctctgtga
     4021 tgcccctgtc aggccttcca gacatgcaca gctgcttgag ctccagtgga ctctaatgac
     4081 aaatatcact gcctgggcca tcttcctgag aaatatccct tagcctgaaa tccacaccaa
     4141 agggagactt ccagaaggat ccgtgtgaag gtcccatacc ctggttccat gtgtcatgtg
     4201 gaaactattg ccagtcttag acagaagggt gcatgtatgc aggatttttc tttttgtccc
     4261 aatagtaaat taagagcagg cagctcccag gctccatgga atatgtaatg aaggacaatg
     4321 tcttcttgga cgaaaatgag agctcctgtc ttcatttgaa gctctggtgt acagtatttc
     4381 aaagtaaaag aaatagtttg agaagtgcgc agctccattt aacactatgg aacattccac
     4441 tttgagcatt gttttcctaa ctgccccgca attccatgtt ttcaagttaa cgtgtatagt
     4501 gaattgtgtc atcctttgct ttgcaggcac tggtggcttg ccgtttgcta atttatttat
     4561 cacagaggca tctgtatttg ctattgacat ggctttatta gataccatag tgactcatat
     4621 aagctggttt cttttataaa ataaaaaaaa atcacatgat tgtatcattt cccagtgaag
     4681 ccatttcatg attagcaata tgggttgtat atttggctgc acgtctttgg gtatctatca
     4741 gttgttgacc tgaacctatc aaacagctac aacatatctg atctgttgaa tcaagatatc
     4801 agccagcctg aacaggatcc agtgggctat tttattgtca gatatttttt gtggttcatt
     4861 tatttttact gtagaaaaga agatagaata tttatctaaa tgcacatgta taaatgatac
     4921 acattatctc catgtatata gtacacgtat gcacccagcc atgtaagtgt acacacatac
     4981 atacgcatga gtgtaaatgt gtgtttatta attggcagtg acccaaatct cttccataag
     5041 atttaaaagc aagttcaggg atacatggac agagaagaaa tgggtcaaat atatgttata
     5101 tggataatta aattatatgg gaatgctaag aatcagttta tgaagtcatt gatgcaactt
     5161 gaggtagtct tagaatttag gaaatgaatt tactaaaaaa atttgaagga tcaaaaattg
     5221 gattttaagt gtcagacttt gagcttgttt taatggtcag gatatgcaaa aatgcatagg
     5281 aaatagagtg caaatgtatt actactatct tcaataaatt ttaactgagt aatttcagtt
     5341 ttattctgac agtaataaag ctctggttat attttagttt gtatatttac ttcacttagt
     5401 tatgttttgt ttaattctta ttttcttcta aggagaataa gagacaaatt atatcatgtt
     5461 aaagctaata aaatcattgt taaaaggtct taagaaataa tattatatag aaaaactatg
     5521 gttttagaga gtgggacaaa gaacattaaa gcccagcagt aacaatgccc atggctttgt
     5581 tttactttaa agggaacctg tctgtttata attttgcaag gcaaataaca gttgtgtcct
     5641 gaaattttgt gttttaagaa tttaatattt ttctggaaat gattttattt aactttaagc
     5701 aataactagg accataatta agttttaatc aaaatgaagg cttgcttcaa acaccatgaa
     5761 agtgtttgat cacacacaca cacacacaca cacacacaca cacacacaca cacacactta
     5821 ctgagacgag acataagggg gaaatcacat cccctttaga gatgattgta ttttatctga
     5881 aggaccaaga gtgttaatta acttactaaa gcttaccttc cactgaagag agatctcttg
     5941 tgaaccattt aacttttgca ctttgaagga aaggaaagga attactcaga aggaagtgag
     6001 acggtccaaa tggaatgctg aattttacaa acacagtcac agactgttgg aaattggaga
     6061 agagtgaacg gataacagga ttcgaaacca aacacactgg tggttatggc aggacattgg
     6121 atttcatcat agtccatata gtgaccgagg ttgaataacc ttgactttca aatcctgttt
     6181 tggtagttgg acttcattat cttggtgatt ctagtcattt tacaatgttt tgtatctggt
     6241 catttactgt aatcacattt tttatatctg tacagtgaca ctttttgcag ttgtggggta
     6301 gtgtgtatca ctgtgcatct tgcatcatcg aaactactac catgatacta tccatcgata
     6361 atattaatat tacttgaaaa tagacaaagg taaagaaaag gggtctatat gatgtgcagt
     6421 tttgtgcctt tatgtacttg ccttgttctt cgttgaatgt gtgaaattct gtactgtgga
     6481 ttttcctaga gtagagagta gagtcatgca ttaaaatagg ctgtatccct gacaccttta
     6541 aactactgag aataatgtgg ttgggccgtg taaatcagtg ttcaaagttc taatgacatg
     6601 acatgtgctc tgttttttaa catttcagta ccaccacaca ttaattaata ctcctgtaat
     6661 agataagaag tcattaatta agttccaaaa gaaagggcca ttgcttgcat tcctttgaat
     6721 ttaatgttgc ccttgtgcac tgtgttaata ctgttcggaa tggattgaac attgtgattc
     6781 ccgcctttta agaggagaaa gagggagagg gcaaagtatt tgatgctctt aaaatgtaca
     6841 tatttgggtt ctatctgaaa ttatttaaaa tgcataattc acatttttgt aatcattcta
     6901 tgcaattttg tggcatgatg tttcttccac ttgtaatttt atgtgctttc atcacacaaa
     6961 aattgtccaa aggaaacaat aaaaatttct taacacag
//
