LOCUS       NM_001081407            4853 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus phospholipase B1 (Plb1), transcript variant 1, mRNA.
ACCESSION   NM_001081407 XM_979420
VERSION     NM_001081407.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Cheng,X., Zhu,H., Bai,S., Zou,Y., Xia,Z. and Yang,R.
  TITLE     Pathogenicity of phospholipase B1 of Trichosporon asahii in
            immunosuppressed mice
  JOURNAL   Mycoses 66 (6), 467-476 (2023)
   PUBMED   36680377
  REMARK    GeneRIF: Pathogenicity of phospholipase B1 of Trichosporon asahii
            in immunosuppressed mice.
REFERENCE   3  (bases 1 to 4853)
  AUTHORS   Asano,A., Nelson-Harrington,J.L. and Travis,A.J.
  TITLE     Phospholipase B is activated in response to sterol removal and
            stimulates acrosome exocytosis in murine sperm
  JOURNAL   J Biol Chem 288 (39), 28104-28115 (2013)
   PUBMED   23943622
  REMARK    GeneRIF: sperm surface phospholipase B is activated in response to
            sterol removal
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK161095.1, BC119077.1, AC102372.8 and AK087596.1.
            
            On Feb 6, 2007 this sequence version replaced XM_979420.1.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and it encodes the longest protein (isoform 1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            transcript and genomic sequence to make the sequence consistent
            with the reference genome assembly. The genomic coordinates used
            for the transcript record were based on alignments.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMN00849374, SAMN00849375
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2562              AK161095.1         2-2563
            2563-3089           BC119077.1         1995-2521
            3090-3171           AC102372.8         73683-73764
            3172-3276           AC102372.8         74361-74465
            3277-3372           AC102372.8         75910-76005
            3373-3444           AC102372.8         76549-76620
            3445-3510           AC102372.8         81189-81254
            3511-3571           AC102372.8         85021-85081
            3572-3630           AC102372.8         85598-85656
            3631-3698           AC102372.8         87963-88030
            3699-3742           AC102372.8         88495-88538
            3743-3842           AC102372.8         88756-88855
            3843-3894           AC102372.8         92741-92792
            3895-3975           AC102372.8         93657-93737
            3976-4041           AC102372.8         94812-94877
            4042-4136           AC102372.8         95287-95381
            4137-4853           AK087596.1         48-764
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 17.33 cM"
     gene            1..4853
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /note="phospholipase B1"
                     /db_xref="GeneID:665270"
                     /db_xref="MGI:MGI:1922406"
     exon            1..158
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            159..364
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             304..4740
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /EC_number="3.1.1.3"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.1.4"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; phospholipase B1, membrane-associated; PLB/LIP;
                     phospholipase B/lipase; phospholipase A2;
                     lysophospholipase; triacylglycerol lipase"
                     /codon_start=1
                     /product="phospholipase B1, membrane-associated isoform 1
                     precursor"
                     /protein_id="NP_001074876.1"
                     /db_xref="CCDS:CCDS39060.1"
                     /db_xref="GeneID:665270"
                     /db_xref="MGI:MGI:1922406"
                     /translation="MELYPGVSPVGLLLLLLLGQGPSQIHGSSGENTLAWQSQQVFWT
                     LKNFPFPCKPKKLELSVLSESVHSLRPSDIKLVAAIGNPEIPLAPGSGTINMEKPQSI
                     KNQPQDVCMGIMTVLSDIIRHFSPSVLMPTCSPGKGTAVHTTAEDLWIQAKELVRRLK
                     DNPQLDFEKDWKLITVFFSNTSQCHLCPSAQQKSHLMRHMEMLWGVLDYLHHEVPRAF
                     VNLVDLSEVLAMDLQHQETGFSPAPEVCKCTETTTLSKAVMQWSYQEAWEDLLASSKF
                     NKHETFAVVFQPFFDEIEPPLKRSSPQDPTTLALRIWNSMMEPVGQKDGLLNTAERKT
                     MKCPSEESPYLFTYKNSNYQARRLKPITKLQMKEGSEFTCPDKNPSNSIPTTVHSLRP
                     ADIKIIGALGDSLTAGNGAGASPWNILDVLTEYRGLSWSVGGDETIKTVTTLPNILRE
                     FNPSLKGFSVGTGKESTSRASFNQAVAGAKSDGLAGQARKLVDLMKADKTINFQEDWK
                     IITVFIGGNDLCASCSNSTRFSPQNFIDNIKNALDILHAEVPRAFVNMAMVMEITPLR
                     ELFNEPTVSCPRNILSRLCPCVLGLGDNSEELSSLVQRNRDYQKKTEELINSGRYDTR
                     DNFTVVVQPLFENVSMPRTPEGVPDKSFFAPDCFHFNAKTHARSAIALWKNMLEPVGH
                     KTRHNNFEIKAPIVCPNQASPFLSTTKNSNLGNGTWMVCEERAPSASPPTSVHTLRPA
                     DIQVVAALGDSLTAGNGISSQEGNLTDVSTQYRGLSYSAGGDKTLENVTTLPNILRKF
                     NGNLTGYSVGTGDSSSANAFLNQAVPGAKAENLTSQVRTLVQKMKSDNRVNFNRDWKV
                     ITVMIGASDLCDFCTDSNHYSAANFFDHLQNALDILHKEVPRALVNLVDFINPSIIRE
                     VFLKNPDKCPVNQSSVLCNCVLTPRKDSYELARLEAFTKSYQSSMLQLVESGRYDTRE
                     DFSVVLQPFLLNTKLPVLENGKPDTSFFAPDCIHLNQKFHTQLARALWANMLEPLGKK
                     TDTLDPKGHISLACPTKDQPFLRTFRNSNYKYPTKPAIENWGSDFLCTEKSPSSQVPT
                     SVHELRPADIKVVAAMGDFLTTATGARPSGYKRLATPWRGLSWSIGGDGKLETHTTLP
                     NILKKFNPSITGFSTGTLDNKAGLNVAEEGARAQDMPAQAKTLVKKMKSTPTINLQED
                     WKLITLLIGNNDLCLYCENPEDNSTKEYVKYIQQALDILYEELPRVFINVVEVMELAG
                     LHHVQGGKCAMPLAVQKNCSCLRHSQNLTAMQELKKLNWNLQSGISELSYWHRYMERE
                     DFAVTVQPFFRNTFIPLNEREGLDLTFFSEDCFYFSDRGHAEMAIALWNNMLEPVGWK
                     TSSNNFIYNRTKLKCPSPERPFLYTLRNSQLLPDKAEEPSNALYWAVPVAAIGGLAVG
                     ILGVMLWRTVKPVQQEEEEEDTLPNTSVTQDAVSEKRLKAGN"
     exon            365..426
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            427..499
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            500..558
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            559..605
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            606..649
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            650..740
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            741..792
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            793..879
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            880..942
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            943..1022
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1023..1095
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1096..1194
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1195..1251
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1252..1323
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1324..1398
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1399..1462
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1463..1521
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1522..1595
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1596..1639
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1640..1748
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1749..1800
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1801..1881
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1882..1947
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1948..2048
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2049..2130
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2131..2235
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2236..2331
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2332..2403
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2404..2442
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2443..2503
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2504..2562
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2563..2636
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2637..2680
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2681..2789
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2790..2841
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2842..2922
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2923..2988
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2989..3089
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3090..3171
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3172..3276
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3277..3372
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3373..3444
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3445..3510
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3511..3571
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3572..3630
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3631..3698
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3699..3742
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3743..3842
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3843..3894
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3895..3975
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3976..4041
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4042..4136
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4137..4215
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4216..4320
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4321..4416
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4417..4491
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4492..4853
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4823..4828
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /note="hexamer: GATAAA"
     polyA_site      4844
                     /gene="Plb1"
                     /gene_synonym="4632413E21Rik; 4930433E17Rik;
                     4930539A06Rik"
                     /note="major polyA site"
ORIGIN      
        1 aagaaagcag tagaagccag cactcacact ctgttgagac aagcagtggg agaaggtatg
       61 aggatccatt gctgtctgtt ggattcagca aactactgac cttcaagaac ttttaatggc
      121 atggtgggaa cagaagccac attgaaggac agggacagcc agccactgcc ccctttcaaa
      181 ggtgaacaca gtaaaatata cgacaggtgt gctatcagat cccagagggg cataaaagcg
      241 ggaggcagag aggtaactca gccatctgtg gaaggaagcc ccagacacct acaacactga
      301 gggatggagc tgtacccagg tgtgtccccg gtgggactgc tgctgctgct actgctggga
      361 caagggccct cccaaatcca tggctcttct ggagagaaca cattggcatg gcagtcccag
      421 caagtgtttt ggaccctgaa gaattttcca ttcccttgca agccaaagaa gttagaattg
      481 agtgtgcttt ctgagtcagt tcactctctg agaccctcag acattaaact cgtggcagcc
      541 atcggcaacc cagaaattcc tctagcccct gggtctggca cgatcaacat ggagaaacct
      601 caaagcatca agaaccaacc acaggatgta tgcatgggaa tcatgacagt tctttcagat
      661 atcatcagac atttcagtcc ttctgtcctg atgcccacgt gttctcctgg gaagggcact
      721 gcagtccaca ctactgctga agatttgtgg attcaggcta aagagctggt gaggcgcctg
      781 aaggacaacc cgcaacttga ctttgagaag gactggaaac tcatcactgt gttcttcagt
      841 aacacaagcc agtgtcacct gtgtccctct gctcagcaga aaagtcactt gatgaggcac
      901 atggagatgc tgtggggggt gctggattac ctgcatcatg aggtccccag agcatttgtg
      961 aatttggtgg atctctctga ggttttggcc atggatcttc agcatcaaga gactggcttc
     1021 agccctgcac cagaggtttg caaatgcaca gagacaacga cgctatccaa agctgtcatg
     1081 cagtggtcct atcaggaagc ctgggaagat cttctggcct ctagcaagtt caacaagcat
     1141 gagaccttcg cggtggtttt ccagcctttc ttcgatgaaa ttgaaccacc cttgaaaagg
     1201 tcctcgcccc aggatcccac cacactcgcc ctcaggatct ggaatagcat gatggaacca
     1261 gtgggtcaaa aggatgggct actcaatact gcagagagaa agacaatgaa atgtccctct
     1321 gaggagagtc cctatctgtt cacctacaaa aatagcaact accaggccag acgactgaaa
     1381 cctataacaa agcttcagat gaaagaagga tcagaattca cctgtcctga caagaacccc
     1441 tcgaactcaa tccccacaac agttcacagc ttgcggccag ctgatatcaa gatcatagga
     1501 gcattgggtg actcgctcac ggcaggcaat ggagctgggg ccagcccttg gaatatcttg
     1561 gatgtcttaa ctgaataccg aggcctgtcc tggagtgtgg gcggggatga gaccatcaaa
     1621 accgtgacca ccctacccaa cattctccgg gaattcaacc cctccttgaa gggcttttct
     1681 gttggcactg ggaaagaaag cacttcccga gcatccttca accaggccgt agcaggagcc
     1741 aaatctgatg gcttagctgg ccaggccaga aagctggtgg acctgatgaa ggcagacaag
     1801 acaataaact ttcaggaaga ctggaagata atcactgtgt ttataggagg caatgacctc
     1861 tgtgcttcct gcagtaactc gactcgcttt tctccccaaa acttcataga caacatcaag
     1921 aacgccctgg acatcctcca tgcagaggtt ccacgggcct ttgtgaacat ggcaatggtg
     1981 atggagatca cccccttgag agaactattc aatgaaccta cagtctcatg cccacggaac
     2041 atcctcagcc gactgtgtcc ttgtgtcctg ggccttggtg ataactcaga agaactttcc
     2101 agcctcgttc aaaggaacag ggattatcag aagaaaactg aagagctcat taatagcgga
     2161 cgatatgaca cgagggataa tttcactgtg gttgtccagc ccttatttga aaatgtgtcc
     2221 atgccacgga ccccggaggg cgtgcccgac aagtctttct ttgcccctga ctgtttccac
     2281 tttaatgcca agactcatgc ccgctcagcc attgccctct ggaagaacat gctggaacct
     2341 gtgggccaca agacaagaca taataatttt gaaatcaagg cccctatcgt gtgtccgaac
     2401 caggcctcgc catttctgag cactaccaag aacagcaacc tgggtaatgg aacctggatg
     2461 gtgtgcgagg agagagcccc ttctgcctca ccaccaacct cagtgcatac cctgagacct
     2521 gcagacattc aagttgtggc agctctggga gactctctga ctgctggcaa tggaatcagc
     2581 tcccaagaag gtaacctcac cgatgtctcc acacagtatc gaggactgtc ctatagtgct
     2641 ggtggggaca agaccctgga gaatgtgacc actttgccca atatcctacg gaaatttaat
     2701 ggaaatctca caggctactc agtaggaacc ggtgactcca gttccgcaaa cgctttcctt
     2761 aaccaggctg tccctggggc gaaggctgag aaccttacaa gccaagtccg gactctggtt
     2821 cagaagatga agagtgacaa cagagtgaac ttcaaccgag actggaaggt catcacggtg
     2881 atgatcggcg ccagtgactt gtgtgacttc tgcacggatt cgaatcatta ctctgcagcc
     2941 aatttttttg accatctcca aaatgccttg gacattctac ataaggaggt acccagagcc
     3001 ctggtcaacc ttgtggactt cattaacccc agtatcattc gggaagtatt cctgaagaac
     3061 ccagacaagt gcccagtgaa tcagtccagt gtcctgtgca actgtgttct gaccccgagg
     3121 aaggattcct atgagctggc aaggttggag gccttcacca aatcctacca gagtagcatg
     3181 ctccaactgg ttgagtcagg ccgctacgac actcgagagg acttctctgt ggtactgcag
     3241 ccctttctcc tcaacaccaa gctccctgtc ctagagaacg ggaaaccaga tacatccttc
     3301 tttgccccag actgcatcca cctaaaccag aagttccaca ctcagctcgc gagagccctc
     3361 tgggccaata tgcttgaacc cctgggaaag aaaactgata ctttggaccc aaaaggacac
     3421 atatctttgg cctgccccac caaggatcag cccttcctga gaaccttccg aaacagtaac
     3481 tacaagtatc ctaccaagcc agccattgag aactggggca gtgacttcct gtgcacagag
     3541 aagagccctt ccagccaggt acccacctca gttcatgagc tccgaccagc agacatcaag
     3601 gtggtggcag caatgggtga ctttctgact acagccaccg gagctcggcc aagtggatat
     3661 aaaaggctag ccacgccctg gagggggctg tcttggagca ttggaggaga tggaaagttg
     3721 gagacccata ccacacttcc caacatcctg aagaagttca acccttccat cactggattc
     3781 tctactggta ccctggacaa caaggcagga ttaaatgtgg cagaagaagg agccagagct
     3841 caggacatgc cggcccaggc taagaccctg gtgaagaaga tgaaaagcac acctacaatc
     3901 aacctacagg aagactggaa gctgattaca ctcctcattg gaaacaacga cctgtgtctt
     3961 tactgtgaga atccggagga caactcaact aaagagtatg tcaagtacat ccagcaagcc
     4021 ttggacatcc tctatgagga gcttcccaga gttttcatca atgtggtgga agtcatggag
     4081 ctggctggcc tgcaccacgt ccaaggtggg aaatgtgcca tgccactggc agttcagaaa
     4141 aactgcagtt gcttaagaca ctcccaaaac ctcacagcga tgcaggagct gaagaaacta
     4201 aactggaacc tccagagcgg catctccgag ctctcctatt ggcaccggta catggagcgt
     4261 gaggacttcg cagtcactgt gcagcctttc ttccggaata cctttatccc actgaatgag
     4321 cgtgagggcc tggacctcac tttcttctct gaagactgtt tctacttctc agaccgtggg
     4381 catgctgaga tggccattgc cctctggaat aacatgctgg aaccagtggg ctggaagaca
     4441 tcctccaata acttcatata caacagaacc aaactcaagt gcccctcacc tgaaaggcct
     4501 ttcctctaca ccctccggaa tagtcagctt cttccagaca aggctgaaga accctccaat
     4561 gcactctact gggcagtgcc agtggcagca ataggtggcc tggcagttgg catccttgga
     4621 gtgatgttgt ggagaactgt gaaacccgtc caacaggagg aggaggagga ggacactctt
     4681 ccaaatacaa gtgtgaccca ggatgctgta tcagaaaaga ggctcaaagc tgggaactag
     4741 gtactgaaca gtaattaagt aacctcaggg atggggactg cctgccttcc tctcctggga
     4801 gttcatgatc tctttttgga aagataaaaa agaaagagag gccatactca gtg
//
