LOCUS       NM_008872               2570 bp    mRNA    linear   ROD 04-OCT-2024
DEFINITION  Mus musculus plasminogen activator, tissue (Plat), mRNA.
ACCESSION   NM_008872
VERSION     NM_008872.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Prunotto,P., Marie,P., Lebouvier,L., Hommet,Y., Vivien,D. and
            Ali,C.
  TITLE     The Janus face of endogenous neuronal tPA: promoting
            self-protection and worsening the death of neighboring neurons
  JOURNAL   Cell Death Dis 15 (4), 261 (2024)
   PUBMED   38609369
  REMARK    GeneRIF: The Janus face of endogenous neuronal tPA: promoting
            self-protection and worsening the death of neighboring neurons.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2570)
  AUTHORS   Ahmad,M., Haffner-Luntzer,M., Schoppa,A., Najafova,Z., Lukic,T.,
            Yorgan,T.A., Amling,M., Schinke,T. and Ignatius,A.
  TITLE     Mechanical induction of osteoanabolic Wnt1 promotes osteoblast
            differentiation via Plat
  JOURNAL   FASEB J 38 (4), e23489 (2024)
   PUBMED   38407813
  REMARK    GeneRIF: Mechanical induction of osteoanabolic Wnt1 promotes
            osteoblast differentiation via Plat.
REFERENCE   4  (bases 1 to 2570)
  AUTHORS   Dai,W., Zhang,H., Lund,H., Zhang,Z., Castleberry,M., Rodriguez,M.,
            Kuriakose,G., Gupta,S., Lewandowska,M., Powers,H.R., Valmiki,S.,
            Zhu,J., Shapiro,A.D., Hussain,M.M., Lopez,J.A., Sorci-Thomas,M.G.,
            Silverstein,R.L., Ginsberg,H.N., Sahoo,D., Tabas,I. and Zheng,Z.
  TITLE     Intracellular tPA-PAI-1 interaction determines VLDL assembly in
            hepatocytes
  JOURNAL   Science 381 (6661), eadh5207 (2023)
   PUBMED   37651538
REFERENCE   5  (bases 1 to 2570)
  AUTHORS   Lijnen,H.R., van Hoef,B., Beelen,V. and Collen,D.
  TITLE     Characterization of the murine plasma fibrinolytic system
  JOURNAL   Eur J Biochem 224 (3), 863-871 (1994)
   PUBMED   7523120
REFERENCE   6  (bases 1 to 2570)
  AUTHORS   Kahn,T., Friesl,H., Copeland,N.G., Gilbert,D.J., Jenkins,N.A.,
            Gissmann,L., Kramer,J. and zur Hausen,H.
  TITLE     Molecular cloning, analysis, and chromosomal localization of a
            mouse genomic sequence related to the human papillomavirus type 18
            E5 region
  JOURNAL   Mol Carcinog 6 (2), 88-99 (1992)
   PUBMED   1326990
REFERENCE   7  (bases 1 to 2570)
  AUTHORS   Rickles,R.J., Darrow,A.L. and Strickland,S.
  TITLE     Differentiation-responsive elements in the 5' region of the mouse
            tissue plasminogen activator gene confer two-stage regulation by
            retinoic acid and cyclic AMP in teratocarcinoma cells
  JOURNAL   Mol Cell Biol 9 (4), 1691-1704 (1989)
   PUBMED   2542775
REFERENCE   8  (bases 1 to 2570)
  AUTHORS   Rickles,R.J., Darrow,A.L. and Strickland,S.
  TITLE     Molecular cloning of complementary DNA to mouse tissue plasminogen
            activator mRNA and its expression during F9 teratocarcinoma cell
            differentiation
  JOURNAL   J Biol Chem 263 (3), 1563-1569 (1988)
   PUBMED   2826484
REFERENCE   9  (bases 1 to 2570)
  AUTHORS   Huarte,J., Belin,D., Vassalli,A., Strickland,S. and Vassalli,J.D.
  TITLE     Meiotic maturation of mouse oocytes triggers the translation and
            polyadenylation of dormant tissue-type plasminogen activator mRNA
  JOURNAL   Genes Dev 1 (10), 1201-1211 (1987)
   PUBMED   3123315
REFERENCE   10 (bases 1 to 2570)
  AUTHORS   Rajput,B., Marshall,A., Killary,A.M., Lalley,P.A., Naylor,S.L.,
            Belin,D., Rickles,R.J. and Strickland,S.
  TITLE     Chromosomal assignments of genes for tissue plasminogen activator
            and urokinase in mouse
  JOURNAL   Somat Cell Mol Genet 13 (5), 581-586 (1987)
   PUBMED   2821634
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC121835.2, CJ146601.1,
            AK135965.1 and DV644940.1.
            
            On Jul 10, 2015 this sequence version replaced NM_008872.2.
            
            Summary: This gene encodes a key enzyme of the fibrinolytic
            pathway. The encoded protein undergoes proteolytic processing by
            plasmin to generate a heterodimeric serine protease that cleaves
            the proenzyme plasminogen to produce plasmin, a protease that is
            required to break down fibrin clots. Additionally, the encoded
            protein is involved in other biological processes such as synaptic
            plasticity, cell migration and tissue remodeling. Mice lacking the
            encoded protein display a reduction in long-term potentiation in
            hippocampus and conversely, transgenic mice overexpressing the
            encoded protein have increased and prolonged long-term
            potentiation. [provided by RefSeq, Jul 2015].
            
            Sequence Note: Sequence Note: This RefSeq record was created from
            transcript and genomic sequence data to make the sequence
            consistent with the reference genome assembly. The genomic
            coordinates used in the 5' UTR of the transcript record were based
            on data published in PMID: 2542775.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC057967.1, AK135965.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-5                 AC121835.2         22431-22435         c
            6-32                CJ146601.1         2-28
            33-2474             AK135965.1         3-2444
            2475-2570           DV644940.1         184-279
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 11.42 cM"
     gene            1..2570
                     /gene="Plat"
                     /gene_synonym="D8Ertd2e; tPA"
                     /note="plasminogen activator, tissue"
                     /db_xref="GeneID:18791"
                     /db_xref="MGI:MGI:97610"
     exon            1..107
                     /gene="Plat"
                     /gene_synonym="D8Ertd2e; tPA"
                     /inference="alignment:Splign:2.1.0"
     exon            108..195
                     /gene="Plat"
                     /gene_synonym="D8Ertd2e; tPA"
                     /inference="alignment:Splign:2.1.0"
     CDS             133..1812
                     /gene="Plat"
                     /gene_synonym="D8Ertd2e; tPA"
                     /EC_number="3.4.21.68"
                     /note="t-plasminogen activator"
                     /codon_start=1
                     /product="tissue-type plasminogen activator preproprotein"
                     /protein_id="NP_032898.2"
                     /db_xref="CCDS:CCDS22183.1"
                     /db_xref="GeneID:18791"
                     /db_xref="MGI:MGI:97610"
                     /translation="MKRELLCVLLLCGLAFPLPDQGIHGRFRRGARSYRATCRDEPTQ
                     TTYQQHQSWLRPMLRSSRVEYCRCNSGLVQCHSVPVRSCSEPRCFNGGTCQQALYFSD
                     FVCQCPDGFVGKRCDIDTRATCFEEQGITYRGTWSTAESGAECINWNSSVLSLKPYNA
                     RRPNAIKLGLGNHNYCRNPDRDLKPWCYVFKAGKYTTEFCSTPACPKGKSEDCYVGKG
                     VTYRGTHSLTTSQASCLPWNSIVLMGKSYTAWRTNSQALGLGRHNYCRNPDGDARPWC
                     HVMKDRKLTWEYCDMSPCSTCGLRQYKRPQFRIKGGLYTDITSHPWQAAIFVKNKRSP
                     GERFLCGGVLISSCWVLSAAHCFLERFPPNHLKVVLGRTYRVVPGEEEQTFEIEKYIV
                     HEEFDDDTYDNDIALLQLRSQSKQCAQESSSVGTACLPDPNLQLPDWTECELSGYGKH
                     EASSPFFSDRLKEAHVRLYPSSRCTSQHLFNKTVTNNMLCAGDTRSGGNQDLHDACQG
                     DSGGPLVCMINKQMTLTGIISWGLGCGQKDVPGVYTKVTNYLDWIHDNMKQ"
     exon            196..238
                     /gene="Plat"
                     /gene_synonym="D8Ertd2e; tPA"
                     /inference="alignment:Splign:2.1.0"
     exon            239..376
                     /gene="Plat"
                     /gene_synonym="D8Ertd2e; tPA"
                     /inference="alignment:Splign:2.1.0"
     exon            377..487
                     /gene="Plat"
                     /gene_synonym="D8Ertd2e; tPA"
                     /inference="alignment:Splign:2.1.0"
     exon            488..662
                     /gene="Plat"
                     /gene_synonym="D8Ertd2e; tPA"
                     /inference="alignment:Splign:2.1.0"
     exon            663..754
                     /gene="Plat"
                     /gene_synonym="D8Ertd2e; tPA"
                     /inference="alignment:Splign:2.1.0"
     exon            755..929
                     /gene="Plat"
                     /gene_synonym="D8Ertd2e; tPA"
                     /inference="alignment:Splign:2.1.0"
     exon            930..1015
                     /gene="Plat"
                     /gene_synonym="D8Ertd2e; tPA"
                     /inference="alignment:Splign:2.1.0"
     exon            1016..1211
                     /gene="Plat"
                     /gene_synonym="D8Ertd2e; tPA"
                     /inference="alignment:Splign:2.1.0"
     exon            1212..1348
                     /gene="Plat"
                     /gene_synonym="D8Ertd2e; tPA"
                     /inference="alignment:Splign:2.1.0"
     exon            1349..1489
                     /gene="Plat"
                     /gene_synonym="D8Ertd2e; tPA"
                     /inference="alignment:Splign:2.1.0"
     exon            1490..1653
                     /gene="Plat"
                     /gene_synonym="D8Ertd2e; tPA"
                     /inference="alignment:Splign:2.1.0"
     exon            1654..2554
                     /gene="Plat"
                     /gene_synonym="D8Ertd2e; tPA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2522..2527
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Plat"
                     /gene_synonym="D8Ertd2e; tPA"
                     /note="hexamer: AATAAA"
     polyA_site      2546
                     /gene="Plat"
                     /gene_synonym="D8Ertd2e; tPA"
                     /note="major polyA site"
ORIGIN      
        1 ccttcaaatt ggctggagct gttcagggag acagagcctg caggaactca agagctcagc
       61 gtcaaggaga ggcccctgca agagtgggaa agaagcaagc actctcggga cacagaagaa
      121 acggggagca aaatgaagag agagctgctg tgtgtactgc tgctttgtgg actggctttc
      181 ccattgcctg accagggaat acatgggagg ttcagaagag gagcccggtc ctacagagcg
      241 acctgcagag atgagccaac gcagacaact taccaacagc accagtcgtg gctccgaccc
      301 atgctcagaa gcagccgggt ggaatattgc cggtgcaaca gcggcctggt acaatgccac
      361 tcagtgcctg tccgaagttg cagcgaacca agatgcttca atggggggac gtgtcagcag
      421 gccctgtatt tctctgactt tgtctgccag tgccctgatg gatttgtagg gaaacgctgt
      481 gacatagata ccagagcaac atgctttgag gagcagggca tcacctacag aggcacgtgg
      541 agcacagcag aaagtggggc tgagtgcatc aactggaata gcagtgttct gtcgctgaag
      601 ccctacaatg caaggaggcc aaatgccatc aagctgggcc ttgggaatca caattactgc
      661 agaaacccag accgagactt gaagccctgg tgctatgtct ttaaggcagg gaagtatacc
      721 acggagttct gtagcacacc agcttgccct aagggaaaaa gcgaggactg ctatgttggg
      781 aaaggtgtga cttaccgtgg cacccacagc ctcaccacat cccaggcctc ctgcctcccc
      841 tggaattcca tagtcctcat gggcaagagt tacacagcgt ggaggaccaa ctcccaggca
      901 ctcggcctgg gcagacacaa ttattgtcgg aatccagatg gtgatgccag accttggtgc
      961 catgtgatga aggaccgaaa gctgacgtgg gaatactgtg acatgtcccc atgctccacc
     1021 tgtggcctga ggcagtacaa acggcctcag tttagaatta aaggaggact ctacacagac
     1081 atcacctcac acccttggca ggctgccatc tttgtcaaga acaagaggtc tcctggagag
     1141 agattccttt gtggaggggt gctgatcagt tcctgctggg tgctgtcagc tgcccactgc
     1201 tttctagaga ggttcccccc caatcatctt aaagtggtct tgggcagaac atacagggtg
     1261 gtccccggag aggaagaaca gacatttgag attgaaaaat acatagtcca tgaggaattt
     1321 gatgacgata cttatgacaa cgacatcgca ttactgcagc tgaggtcaca gtccaagcaa
     1381 tgtgcccaag agagcagctc tgttggcact gcctgcctcc ctgaccccaa cctgcagctc
     1441 cctgactgga cagagtgtga gctttctggc tacggcaagc atgaggcatc gtctccattc
     1501 ttctctgacc ggctgaagga ggctcacgtc agactgtacc cgtccagccg ctgcacctca
     1561 cagcatctgt ttaataaaac cgtcacgaac aacatgctgt gcgctggaga cacccggagc
     1621 ggaggcaacc aagacctcca cgatgcatgc cagggtgact cgggaggccc tctggtgtgc
     1681 atgatcaata aacagatgac attgactggc attatcagct ggggcctcgg ctgtgggcag
     1741 aaggatgtgc ctggggtcta cacaaaggtt acgaattacc tagactggat tcacgacaac
     1801 atgaagcaat gacaaagaaa gcccagctcc ttcaatccag aggaggacct gccttcctct
     1861 tcctcttcta cagaagatgc gcctaaaagg ccaagcattc tttacaccat catcttcgtg
     1921 agctgccact cgggagaggg gaggggggtc tttgggagca gatgtagcat ttacctgtga
     1981 caggtacttc acaacttgta agttttaagg atgaaggtct ggctttagaa taaatcctgt
     2041 cagatgagat gacagggaga tgccaacctt cctataactc taggatttta aaaagagagg
     2101 tagaccaaag tctgccctcc tggtccacta tgttgtacac tgaaccacaa gatcatgtct
     2161 caacagcaaa aaaatacaac ttgatctttc aggagtgaaa gtttgcactg gggacaagaa
     2221 tgtgttttta tagttacaca gggggcccca cagggcctcc acgagaagga aggggtatct
     2281 ggccgaatca cagcaccata aaatccttga gatcatgcac tccccatccc tccacactcc
     2341 tcaactcttg ggacatatcc ctttgtatac agtgtaaatg tctttttctt tataaactct
     2401 atagatggtt gggagaactg tatgatttta ataattgatg aataacacta gtatatttat
     2461 attttaatct attttagttt ttactttgtt actataactt tgtattatac tgtacttaaa
     2521 taataaattc agaggtattt ttcacacttt tccaaaaaaa aaaaaaaaaa
//
