LOCUS       NM_025731               1087 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus phospholipase A and acyltransferase 5 (Plaat5),
            transcript variant 1, mRNA.
ACCESSION   NM_025731
VERSION     NM_025731.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Jin,X.H., Uyama,T., Wang,J., Okamoto,Y., Tonai,T. and Ueda,N.
  TITLE     cDNA cloning and characterization of human and mouse
            Ca(2+)-independent phosphatidylethanolamine N-acyltransferases
  JOURNAL   Biochim Biophys Acta 1791 (1), 32-38 (2009)
   PUBMED   19000777
REFERENCE   3  (bases 1 to 1087)
  AUTHORS   Jin,X.H., Okamoto,Y., Morishita,J., Tsuboi,K., Tonai,T. and Ueda,N.
  TITLE     Discovery and characterization of a Ca2+-independent
            phosphatidylethanolamine N-acyltransferase generating the
            anandamide precursor and its congeners
  JOURNAL   J Biol Chem 282 (6), 3614-3623 (2007)
   PUBMED   17158102
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC109225.14 and AK014971.1.
            
            On Feb 16, 2019 this sequence version replaced NM_025731.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC061008.1, AK014971.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849382
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            RefSeq Select criteria           :: based on conservation,
                                                expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-32                AC109225.14        83585-83616         c
            33-1076             AK014971.1         2-1045
            1077-1087           AC109225.14        56904-56914         c
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 5.33 cM"
     gene            1..1087
                     /gene="Plaat5"
                     /gene_synonym="1700055A14Rik; 1700086E08Rik;
                     4921526K24Rik; Hrasls5; HRSL5; iNAT"
                     /note="phospholipase A and acyltransferase 5"
                     /db_xref="GeneID:66727"
                     /db_xref="MGI:MGI:1913977"
     exon            1..294
                     /gene="Plaat5"
                     /gene_synonym="1700055A14Rik; 1700086E08Rik;
                     4921526K24Rik; Hrasls5; HRSL5; iNAT"
                     /inference="alignment:Splign:2.1.0"
     CDS             147..959
                     /gene="Plaat5"
                     /gene_synonym="1700055A14Rik; 1700086E08Rik;
                     4921526K24Rik; Hrasls5; HRSL5; iNAT"
                     /EC_number="3.1.1.4"
                     /EC_number="3.1.1.32"
                     /note="isoform 1 is encoded by transcript variant 1;
                     HRAS-like suppressor 5; Ca-independent N-acyltransferase;
                     H-rev107-like protein 5; Ca(2+)-independent
                     N-acyltransferase; HRAS-like suppressor family, member 5"
                     /codon_start=1
                     /product="phospholipase A and acyltransferase 5 isoform 1"
                     /protein_id="NP_080007.2"
                     /db_xref="CCDS:CCDS29530.1"
                     /db_xref="GeneID:66727"
                     /db_xref="MGI:MGI:1913977"
                     /translation="MGLSPAASGEFGIRLSRVPWPRPTQISKTSSTESSDTQSATGQS
                     TVPHSDSASSQALLVQFLPKQLKQDRRLEQARSFQQGEKPETSLELTPSKKRTELIPT
                     SNSEIESTQKNQAVEGNPRPRPGDLIEIFRIGYEHWAIYVEDDCVVHLAPPSEFEAGS
                     ITSIFSNRAVVKYSRLEDVLHGCSWKINNKLDGTYLPLPVDKIMQRTKNMINKIVQYS
                     LIEGNCEHFVNDLRYGVPRSQQVEHALVEGAKAAGAVLSAVVDSIRPKPITA"
     exon            295..388
                     /gene="Plaat5"
                     /gene_synonym="1700055A14Rik; 1700086E08Rik;
                     4921526K24Rik; Hrasls5; HRSL5; iNAT"
                     /inference="alignment:Splign:2.1.0"
     exon            389..497
                     /gene="Plaat5"
                     /gene_synonym="1700055A14Rik; 1700086E08Rik;
                     4921526K24Rik; Hrasls5; HRSL5; iNAT"
                     /inference="alignment:Splign:2.1.0"
     exon            498..606
                     /gene="Plaat5"
                     /gene_synonym="1700055A14Rik; 1700086E08Rik;
                     4921526K24Rik; Hrasls5; HRSL5; iNAT"
                     /inference="alignment:Splign:2.1.0"
     exon            607..866
                     /gene="Plaat5"
                     /gene_synonym="1700055A14Rik; 1700086E08Rik;
                     4921526K24Rik; Hrasls5; HRSL5; iNAT"
                     /inference="alignment:Splign:2.1.0"
     exon            867..1087
                     /gene="Plaat5"
                     /gene_synonym="1700055A14Rik; 1700086E08Rik;
                     4921526K24Rik; Hrasls5; HRSL5; iNAT"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1081
                     /gene="Plaat5"
                     /gene_synonym="1700055A14Rik; 1700086E08Rik;
                     4921526K24Rik; Hrasls5; HRSL5; iNAT"
                     /note="major polyA site"
ORIGIN      
        1 gtttgacacg cgctccctgg aggcgacgcc ccacctctct cacctccgtt ccagccccgc
       61 tggccccagt gcccatgatc ccggggctag gcggaccctg gtccgcacct ccctgcaggc
      121 gcgttgtccc tgctggcccc agcgggatgg gcctgagccc agcagccagc ggggagttcg
      181 ggatccgcct ttccagggtc ccatggccac gccccacgca gatctcgaag acctcaagta
      241 cagagtcaag tgacacccaa tctgcaactg gacagtcaac tgtaccccac tcagactcag
      301 cgagttctca agcgctgttg gtccagttct tgccgaagca actcaagcaa gaccgccgat
      361 tagaacaggc ccgaagcttt cagcagggag agaagccgga aactagtttg gagctcacac
      421 ccagcaagaa gaggacagag ctgattccaa catcaaattc agagatcgaa agcacacaaa
      481 aaaaccaagc agttgagggc aatccaagac ccagacctgg agacctgatt gagatttttc
      541 gaattggcta tgaacattgg gccatctatg tggaagatga ctgcgtggtc cacctggctc
      601 ccccaagtga gtttgaggca gggagcatca cctctatctt cagcaaccga gctgtggtga
      661 agtacagccg gctggaggat gtgctgcacg gttgctcctg gaagatcaac aacaagctgg
      721 atggcacata cctgccgctg ccggtggaca agatcatgca gcgcacaaaa aacatgataa
      781 acaagatagt tcagtacagc ctaattgagg ggaactgtga gcactttgtc aatgacctca
      841 gatatggtgt gcccaggagc cagcaggtgg agcacgccct ggtggaggga gcgaaggctg
      901 caggagctgt cctctcagca gtagtagata gcataagacc caaacccata actgcttgaa
      961 ggtgctacaa ttctgtgcgg aggaagagct actagcagct gcggagcaaa gaccgccttt
     1021 cttcttcctt gcctttctca ctcagtagcc ctgttaaaaa agattgtgag ttcctggaaa
     1081 actggaa
//
