LOCUS       NM_001416400            3305 bp    mRNA    linear   ROD 14-FEB-2025
DEFINITION  Mus musculus phospholipase A2, group VI (Pla2g6), transcript
            variant 14, mRNA.
ACCESSION   NM_001416400 XM_036159515
VERSION     NM_001416400.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Li,S., Liu,H., Hu,H., Ha,E., Prasad,P., Jenkins,B.C., Das,U.S.,
            Mukherjee,S., Shishikura,K., Hu,R., Rader,D.J., Pei,L., Baur,J.A.,
            Matthews,M.L., FitzGerald,G.A., McReynolds,M.R. and Susztak,K.
  TITLE     Human genetics identify convergent signals in mitochondrial
            LACTB-mediated lipid metabolism in cardiovascular-kidney-metabolic
            syndrome
  JOURNAL   Cell Metab 37 (1), 154-168 (2025)
   PUBMED   39561766
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Jiao,L., Shao,W., Quan,W., Xu,L., Liu,P., Yang,J. and Peng,X.
  TITLE     iPLA2beta loss leads to age-related cognitive decline and
            neuroinflammation by disrupting neuronal mitophagy
  JOURNAL   J Neuroinflammation 21 (1), 228 (2024)
   PUBMED   39294744
  REMARK    GeneRIF: iPLA2beta loss leads to age-related cognitive decline and
            neuroinflammation by disrupting neuronal mitophagy.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3305)
  AUTHORS   Taketomi,Y., Higashi,T., Kano,K., Miki,Y., Mochizuki,C.,
            Toyoshima,S., Okayama,Y., Nishito,Y., Nakae,S., Tanaka,S.,
            Tokuoka,S.M., Oda,Y., Shichino,S., Ueha,S., Matsushima,K.,
            Akahoshi,N., Ishii,S., Chun,J., Aoki,J. and Murakami,M.
  TITLE     Lipid-orchestrated paracrine circuit coordinates mast cell
            maturation and anaphylaxis through functional interaction with
            fibroblasts
  JOURNAL   Immunity 57 (8), 1828-1847 (2024)
   PUBMED   39002541
REFERENCE   4  (bases 1 to 3305)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   5  (bases 1 to 3305)
  AUTHORS   Klement,L., Jansakun,C., Yan,B., Staffer,S., Tuma-Kellner,S.,
            Altamura,S., Muckenthaler,M., Merle,U. and Chamulitrat,W.
  TITLE     Myeloid-specific deletion of group VIA calcium-independent
            phospholipase A2 induces pro-inflammatory LPS response
            predominantly in male mice via MIP-1alpha activation
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1870 (3), 167016 (2024)
   PUBMED   38198970
  REMARK    GeneRIF: Myeloid-specific deletion of group VIA calcium-independent
            phospholipase A2 induces pro-inflammatory LPS response
            predominantly in male mice via MIP-1alpha activation.
REFERENCE   6  (bases 1 to 3305)
  AUTHORS   Su,X., Mancuso,D.J., Bickel,P.E., Jenkins,C.M. and Gross,R.W.
  TITLE     Small interfering RNA knockdown of calcium-independent
            phospholipases A2 beta or gamma inhibits the hormone-induced
            differentiation of 3T3-L1 preadipocytes
  JOURNAL   J Biol Chem 279 (21), 21740-21748 (2004)
   PUBMED   15024020
  REMARK    GeneRIF: iPLA(2)beta and iPLA(2)gamma are required for the
            adipogenic program that drives resting 3T3-L1 cells into adipocytes
            after hormone stimulation
REFERENCE   7  (bases 1 to 3305)
  AUTHORS   Maggi,L.B. Jr., Moran,J.M., Scarim,A.L., Ford,D.A., Yoon,J.W.,
            McHowat,J., Buller,R.M. and Corbett,J.A.
  TITLE     Novel role for calcium-independent phospholipase A(2) in the
            macrophage antiviral response of inducible nitric-oxide synthase
            expression
  JOURNAL   J Biol Chem 277 (41), 38449-38455 (2002)
   PUBMED   12167650
  REMARK    GeneRIF: These findings support an RNA-dependent protein
            kinase-independent signaling role for iPLA(2) in the antiviral
            response of macrophages and the stimulation of inducible nitric
            oxide synthase expression.
REFERENCE   8  (bases 1 to 3305)
  AUTHORS   Balsinde,J., Balboa,M.A. and Dennis,E.A.
  TITLE     Antisense inhibition of group VI Ca2+-independent phospholipase A2
            blocks phospholipid fatty acid remodeling in murine P388D1
            macrophages
  JOURNAL   J Biol Chem 272 (46), 29317-29321 (1997)
   PUBMED   9361012
REFERENCE   9  (bases 1 to 3305)
  AUTHORS   Balboa,M.A., Balsinde,J., Jones,S.S. and Dennis,E.A.
  TITLE     Identity between the Ca2+-independent phospholipase A2 enzymes from
            P388D1 macrophages and Chinese hamster ovary cells
  JOURNAL   J Biol Chem 272 (13), 8576-8580 (1997)
   PUBMED   9079688
REFERENCE   10 (bases 1 to 3305)
  AUTHORS   Kelly,S.S. and Robbins,N.
  TITLE     Statistics of neuromuscular transmitter release in young and old
            mouse muscle
  JOURNAL   J Physiol 385, 507-516 (1987)
   PUBMED   2888882
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC165278.4 and AL591913.14.
            
            On Jan 31, 2023 this sequence version replaced XM_036159515.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR9219382.54525.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-44                AC165278.4         1365-1408           c
            45-294              AL591913.14        129424-129673
            295-510             AL591913.14        134271-134486
            511-694             AL591913.14        135457-135640
            695-882             AL591913.14        138578-138765
            883-979             AL591913.14        140025-140121
            980-1162            AL591913.14        140968-141150
            1163-1271           AL591913.14        141623-141731
            1272-1350           AL591913.14        144737-144815
            1351-1514           AL591913.14        148239-148402
            1515-1665           AL591913.14        149985-150135
            1666-1802           AL591913.14        157719-157855
            1803-1957           AL591913.14        158149-158303
            1958-2563           AL591913.14        159213-159818
            2564-2637           AL591913.14        160251-160324
            2638-3305           AL591913.14        160539-161206
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 37.7 cM"
     gene            1..3305
                     /gene="Pla2g6"
                     /gene_synonym="iPLA(2)beta; iPLA2; iPLA2beta; PNPLA9"
                     /note="phospholipase A2, group VI"
                     /db_xref="GeneID:53357"
                     /db_xref="MGI:MGI:1859152"
     exon            1..44
                     /gene="Pla2g6"
                     /gene_synonym="iPLA(2)beta; iPLA2; iPLA2beta; PNPLA9"
                     /inference="alignment:Splign:2.1.0"
     exon            45..294
                     /gene="Pla2g6"
                     /gene_synonym="iPLA(2)beta; iPLA2; iPLA2beta; PNPLA9"
                     /inference="alignment:Splign:2.1.0"
     CDS             86..2782
                     /gene="Pla2g6"
                     /gene_synonym="iPLA(2)beta; iPLA2; iPLA2beta; PNPLA9"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.1.4"
                     /EC_number="3.1.2.2"
                     /note="isoform 3 is encoded by transcript variant 14; 85
                     kDa calcium-independent phospholipase A2; GVI PLA2;
                     caI-PLA2; group VI phospholipase A2; iPLA2-beta;
                     patatin-like phospholipase domain-containing protein 9;
                     intracellular membrane-associated calcium-independent
                     phospholipase A2 beta; 85/88 kDa calcium-independent
                     phospholipase A2; palmitoyl-CoA hydrolase;
                     2-lysophosphatidylcholine acylhydrolase"
                     /codon_start=1
                     /product="85/88 kDa calcium-independent phospholipase A2
                     isoform 3"
                     /protein_id="NP_001403329.1"
                     /db_xref="GeneID:53357"
                     /db_xref="MGI:MGI:1859152"
                     /translation="MQFFGRLVNTLSSVTNLFSNPFRVKEVSLTDYVSSERVREEGQL
                     ILLQNVSNRTWDCVLVSPRNPQSGFRLFQLESEADALVNFQQFSSQLPPFYESSVQVL
                     HVEVLQHLTDLIRNHPSWTVTHLAVELGIRECFHHSRIISCANSTENEEGCTPLHLAC
                     RKGDSEILVELVQYCHAQMDVTDNKGETAFHYAVQGDNPQVLQLLGKNASAGLNQVNN
                     QGLTPLHLACKMGKQEMVRVLLLCNARCNIMGPGGFPIHTAMKFSQKGCAEMIISMDS
                     NQIHSKDPRYGASPLHWAKNAEMARMLLKRGCDVDSTSSSGNTALHVAVMRNRFDCVM
                     VLLTYGANAGARGEHGNTPLHLAMSKDNMEMVKALIVFGAEVDTPNDFGETPALIASK
                     ISKQLQDLMPISRARKPAFILSSMRDEKRSHDHLLCLDGGGVKGLVIIQLLIAIEKAS
                     GVATKDLFDWVAGTSTGGILALAILHSKSMAYMRGVYFRMKDEVFRGSRPYESGPLEE
                     FLKREFGEHTKMTDVKKPKVMLTGTLSDRQPAELHLFRNYDAPEAVREPRCNQNINLK
                     PPTQPADQLVWRAARSSGAAPTYFRPNGRFLDGGLLANNPTLDAMTEIHEYNQDMIRK
                     LPQGAAAGPGQDGTWVCSCCSCCSCCSCCSCCSCSCCCCCSCCSCCSCCCCCSCCSCC
                     SCCSCCSCCCCCSCCSCCCCCSCCSCCSCCSCCCSCCSCSCSCSYSLSYSRSRVLSVP
                     HSPLPSLGPVLTPIPTCGKSSLTTLSCSSQGQGNKVKKLSIVVSLGTGKSPQVPVTCV
                     DVFRPSNPWELAKTVFGAKELGKMVVDCCTDPDGRAVDRARAWCEMVGIQYFRLNPQL
                     GSDIMLDEVSDAVLVNALWETEVYIYEHREEFQKLVQLLLSP"
     exon            295..510
                     /gene="Pla2g6"
                     /gene_synonym="iPLA(2)beta; iPLA2; iPLA2beta; PNPLA9"
                     /inference="alignment:Splign:2.1.0"
     exon            511..694
                     /gene="Pla2g6"
                     /gene_synonym="iPLA(2)beta; iPLA2; iPLA2beta; PNPLA9"
                     /inference="alignment:Splign:2.1.0"
     exon            695..882
                     /gene="Pla2g6"
                     /gene_synonym="iPLA(2)beta; iPLA2; iPLA2beta; PNPLA9"
                     /inference="alignment:Splign:2.1.0"
     exon            883..979
                     /gene="Pla2g6"
                     /gene_synonym="iPLA(2)beta; iPLA2; iPLA2beta; PNPLA9"
                     /inference="alignment:Splign:2.1.0"
     exon            980..1162
                     /gene="Pla2g6"
                     /gene_synonym="iPLA(2)beta; iPLA2; iPLA2beta; PNPLA9"
                     /inference="alignment:Splign:2.1.0"
     exon            1163..1271
                     /gene="Pla2g6"
                     /gene_synonym="iPLA(2)beta; iPLA2; iPLA2beta; PNPLA9"
                     /inference="alignment:Splign:2.1.0"
     exon            1272..1350
                     /gene="Pla2g6"
                     /gene_synonym="iPLA(2)beta; iPLA2; iPLA2beta; PNPLA9"
                     /inference="alignment:Splign:2.1.0"
     exon            1351..1514
                     /gene="Pla2g6"
                     /gene_synonym="iPLA(2)beta; iPLA2; iPLA2beta; PNPLA9"
                     /inference="alignment:Splign:2.1.0"
     exon            1515..1665
                     /gene="Pla2g6"
                     /gene_synonym="iPLA(2)beta; iPLA2; iPLA2beta; PNPLA9"
                     /inference="alignment:Splign:2.1.0"
     exon            1666..1802
                     /gene="Pla2g6"
                     /gene_synonym="iPLA(2)beta; iPLA2; iPLA2beta; PNPLA9"
                     /inference="alignment:Splign:2.1.0"
     exon            1803..1957
                     /gene="Pla2g6"
                     /gene_synonym="iPLA(2)beta; iPLA2; iPLA2beta; PNPLA9"
                     /inference="alignment:Splign:2.1.0"
     exon            1958..2563
                     /gene="Pla2g6"
                     /gene_synonym="iPLA(2)beta; iPLA2; iPLA2beta; PNPLA9"
                     /inference="alignment:Splign:2.1.0"
     exon            2564..2637
                     /gene="Pla2g6"
                     /gene_synonym="iPLA(2)beta; iPLA2; iPLA2beta; PNPLA9"
                     /inference="alignment:Splign:2.1.0"
     exon            2638..3305
                     /gene="Pla2g6"
                     /gene_synonym="iPLA(2)beta; iPLA2; iPLA2beta; PNPLA9"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3285..3290
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pla2g6"
                     /gene_synonym="iPLA(2)beta; iPLA2; iPLA2beta; PNPLA9"
                     /note="hexamer: AATAAA"
     polyA_site      3305
                     /gene="Pla2g6"
                     /gene_synonym="iPLA(2)beta; iPLA2; iPLA2beta; PNPLA9"
                     /note="major polyA site"
ORIGIN      
        1 agtttgcgga agtaggaggg ctgagaagtg ctgagtaagc cgagagcaga ggggcaggct
       61 gggtctctcc gctgccccac agaggatgca gttcttcgga cgcctcgtca acaccctcag
      121 tagcgtcacc aacttgttct cgaacccatt ccgggtgaag gaggtgtccc tgactgacta
      181 cgtctcaagt gaacgtgtcc gggaggaagg gcagctgatc ctgttacaga atgtctccaa
      241 tcgcacctgg gactgtgtcc tggtcagccc gaggaaccca cagagcggct tccggctctt
      301 ccaactggag tctgaggcag acgccctggt gaacttccag cagttctcct cccagctgcc
      361 gcccttctac gagagctctg tgcaggtcct gcatgtggag gtgcttcagc acctgaccga
      421 cctcatccgg aaccacccca gctggacagt gacacaccta gccgtggagc tcggcatccg
      481 ggagtgcttc catcacagcc gcatcatcag ctgtgccaac agcacagaga atgaggaggg
      541 ctgcacccca ctacatctgg cctgccgcaa gggtgacagt gagatcctgg tggagctggt
      601 acaatactgc cacgcccaga tggatgtcac tgacaacaaa ggagagactg ccttccatta
      661 cgctgtgcaa ggggacaatc cccaggtgct acagctccta gggaagaacg cctcagccgg
      721 cctgaaccag gtaaacaacc aagggctgac tccactgcac ctggcctgca agatgggaaa
      781 gcaggagatg gtgcgcgtcc tgctgctctg taatgcccgc tgcaacatca tggggcccgg
      841 tggcttcccc atccacacag ccatgaagtt ttcccagaag gggtgtgctg aaatgattat
      901 cagcatggac agcaaccaga tccacagcaa ggatcctcgc tacggagcca gcccactcca
      961 ttgggccaag aacgccgaga tggcccgaat gctgctgaag cggggctgtg acgtggacag
     1021 cactagctcc tcagggaaca cagccctgca tgtggcggtg atgcgcaacc gctttgactg
     1081 tgtcatggtg ctgctgacct acggggctaa tgcaggtgcc cgcggagagc acgggaacac
     1141 gccactgcac ctggccatgt cgaaagataa catggagatg gtcaaagccc tcattgtatt
     1201 tggggcagaa gtggacaccc caaacgactt tggggagact cctgcattga tagcctccaa
     1261 gatcagcaag cagcttcagg atctcatgcc catctctcga gcccggaagc cagcgttcat
     1321 cctgagctcc atgagggacg agaagcggag tcacgaccac ctgctctgcc tggacggagg
     1381 gggcgtgaaa ggcctggtca ttatccagct tctcatcgcc atcgagaagg cctcgggagt
     1441 ggccaccaag gacctcttcg actgggtggc aggaaccagc acagggggca tcctggccct
     1501 ggccattctg cacagtaaat ccatggccta tatgcgtggt gtgtacttcc gtatgaagga
     1561 cgaggtgttt cggggctcac ggccctatga gtctgggccc ctggaggagt tcctgaagcg
     1621 ggagtttggg gagcacacca agatgacaga tgtcaaaaaa cccaaggtga tgctgacagg
     1681 gacactgtct gaccggcagc cagcagagct ccacctattc cggaattacg atgctcccga
     1741 agccgttcga gagccccgct gcaaccaaaa cattaacctg aagccaccga ctcagcctgc
     1801 agaccaactg gtatggcgtg cagcccggag cagtggggca gccccaacct atttccggcc
     1861 caatggacgc ttcctggatg gagggctgct ggccaacaac cccacactgg atgccatgac
     1921 tgaaatccat gagtacaatc aggacatgat ccgcaagctc ccacagggag cagctgcagg
     1981 acctggccag gatggaacct gggtctgctc ctgctgctcc tgctgctcct gctgctcctg
     2041 ctgctcctgc tgctcctgct cctgctgctg ctgctgctcc tgctgctcct gctgttcctg
     2101 ctgctgctgc tgctcctgct gctcctgctg ctcctgctgc tcctgctgct cctgctgctg
     2161 ctgctgctcc tgctgctcct gctgctgctg ctgctcctgc tgctcctgct gctcctgctg
     2221 ctcctgctgc tgctcctgtt gttcctgctc ctgctcctgc tcctactccc tctcctactc
     2281 ccgctccagg gtcttatctg tccctcacag ccctctgccc agcctcggtc ctgttctgac
     2341 tcctatccct acctgtggga agtcctctct gaccaccctt tcttgctcat cccagggcca
     2401 gggcaacaag gtgaagaaac tctccatagt cgtttctctg gggacaggaa agtcccctca
     2461 agtgcctgta acctgtgtag atgtctttcg tcccagcaac ccttgggaac tggccaaaac
     2521 tgtttttgga gccaaggaac tgggcaagat ggtcgtggac tgttgcacag atccagatgg
     2581 gcgggctgtg gatcgggccc gggcctggtg cgagatggtc ggcatccagt acttcagact
     2641 gaacccccag ctagggtccg acatcatgct ggacgaggtc agtgatgcag tgctggtcaa
     2701 cgccctctgg gagaccgagg tctacatcta tgagcaccga gaggagttcc agaagcttgt
     2761 ccagctgctg ctgtctccct gagctcccgg ggcctgccag cacactgagg gccaagctgg
     2821 gcctggcagc tccatgtcca tctgacacta ggactcagag cacaaaccgg ttcccacgag
     2881 gcacctctcc tggtccatct gcactttact actcgaggct gaaagcccag agttcccctc
     2941 agcccctttc cctgactatg aaggacaact gactccatca aggaccccac atatcagaga
     3001 tcaacactac ggtgccagtg gtcccaggct ccctggccac tgctgctcac cccttctgcc
     3061 caccccctga tgtcagggac tagagaaaag gggttccacc ctatcctagt ggaatccagg
     3121 ctaatggcat ccagcgccat tcgactttgc ccctcgcacc tgccagcctc tccaccctgc
     3181 cgccaccgca ctaggagccc tcctagctcc caaaggtcaa ccctgtgcat gtgctcttct
     3241 ctggaaggag agtggggagg ggctcagggc cacctcaact gtgaaataaa cgggtttaca
     3301 ctcaa
//
