LOCUS       NM_001363073            3113 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus phospholipase A2, group IVF (Pla2g4f), transcript
            variant 2, mRNA.
ACCESSION   NM_001363073 XM_006499649
VERSION     NM_001363073.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Yun,B., Lee,H., Ghosh,M., Cravatt,B.F., Hsu,K.L., Bonventre,J.V.,
            Ewing,H., Gelb,M.H. and Leslie,C.C.
  TITLE     Serine hydrolase inhibitors block necrotic cell death by preventing
            calcium overload of the mitochondria and permeability transition
            pore formation
  JOURNAL   J Biol Chem 289 (3), 1491-1504 (2014)
   PUBMED   24297180
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Ghosh,M., Loper,R., Ghomashchi,F., Tucker,D.E., Bonventre,J.V.,
            Gelb,M.H. and Leslie,C.C.
  TITLE     Function, activity, and membrane targeting of cytosolic
            phospholipase A(2)zeta in mouse lung fibroblasts
  JOURNAL   J Biol Chem 282 (16), 11676-11686 (2007)
   PUBMED   17293613
REFERENCE   3  (bases 1 to 3113)
  AUTHORS   Ohto,T., Uozumi,N., Hirabayashi,T. and Shimizu,T.
  TITLE     Identification of novel cytosolic phospholipase A(2)s, murine
            cPLA(2){delta}, {epsilon}, and {zeta}, which form a gene cluster
            with cPLA(2){beta}
  JOURNAL   J Biol Chem 280 (26), 24576-24583 (2005)
   PUBMED   15866882
  REMARK    GeneRIF: murine cytosolic phospholipases A2 delta, epsilon, and
            zeta form a gene cluster with cPLA(2)beta
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL844608.18.
            
            On May 5, 2018 this sequence version replaced XM_006499649.3.
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice junction compared to variant 1. The resulting isoform (2)
            has the same N- and C-termini but is 1 aa shorter compared to
            isoform 1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC145428.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164134 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-220               AL844608.18        113879-114098       c
            221-293             AL844608.18        112980-113052       c
            294-430             AL844608.18        112240-112376       c
            431-556             AL844608.18        112038-112163       c
            557-597             AL844608.18        111697-111737       c
            598-640             AL844608.18        111412-111454       c
            641-707             AL844608.18        110950-111016       c
            708-871             AL844608.18        108138-108301       c
            872-985             AL844608.18        107907-108020       c
            986-1023            AL844608.18        107496-107533       c
            1024-1159           AL844608.18        107158-107293       c
            1160-1296           AL844608.18        104935-105071       c
            1297-1519           AL844608.18        104486-104708       c
            1520-1634           AL844608.18        103509-103623       c
            1635-1772           AL844608.18        103186-103323       c
            1773-1880           AL844608.18        103000-103107       c
            1881-2096           AL844608.18        102660-102875       c
            2097-2263           AL844608.18        102130-102296       c
            2264-2450           AL844608.18        100830-101016       c
            2451-3113           AL844608.18        99899-100561        c
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 60.31 cM"
     gene            1..3113
                     /gene="Pla2g4f"
                     /gene_synonym="4732472I07Rik; Gm647; Pla2zeta"
                     /note="phospholipase A2, group IVF"
                     /db_xref="GeneID:271844"
                     /db_xref="MGI:MGI:2685493"
     exon            1..220
                     /gene="Pla2g4f"
                     /gene_synonym="4732472I07Rik; Gm647; Pla2zeta"
                     /inference="alignment:Splign:2.1.0"
     CDS             110..2674
                     /gene="Pla2g4f"
                     /gene_synonym="4732472I07Rik; Gm647; Pla2zeta"
                     /EC_number="3.1.1.4"
                     /note="isoform 2 is encoded by transcript variant 2;
                     cytosolic phospholipase A2 zeta; cPLA2-zeta"
                     /codon_start=1
                     /product="cytosolic phospholipase A2 zeta isoform 2"
                     /protein_id="NP_001350002.1"
                     /db_xref="GeneID:271844"
                     /db_xref="MGI:MGI:2685493"
                     /translation="MPWTLQPKWLAGKGLPLLGAILLRKTEKSEPQWKHRRQETHPYY
                     DLQVKVLRARNIQHTDKLSKADCYVRLWLPTASVSPSQTRTVVNSSDPEWNETFPYQI
                     HGAVKNVLELALYDEDVLDSDNVFSILFDTSTLQLGQPCTKNFTRQQDPKELEVEFTL
                     EKSQTPASEVVTNGVLVAHPCLRIQGTVTGDKTASLGELGSRQIQLAVPGAYEKPQPL
                     QPTSEPGLPVNFTFHVNPVLSPKLHIKLQEQLQVFHSGPSDELEAQTSKMDKASILLS
                     SLPLNEELTKLVDLEEGQQVSLRMKADMSSGDLDLRLGFDLCDGEQEFLDKRKQVASK
                     ALQRVMGLSEALHCDQVPVVAVLGSGGGTRAMTSLYGSLAGLQELGLLDAVTYLSGVS
                     GSSWCISTLYRDPSWSQKALQGPIKYASERVCSSKIGMLSPKQFEYYSREKRAWESRG
                     HSMSFTDLWGLIIEYFLNQEENPAKLSDQQETVSQGQNPYPIYASINVHKNISGDDFA
                     EWCEFTPYEVGFPKYGAYVPTELFGSEFFMGRLLHFWPEPRICYLQGMWGSAFAASLY
                     EIFLKLGGLSLSFLDWHRGSVSVTDDWPKLRKQDPTRLPTRLFTPMSSFSQAVLDIFT
                     SRITCAQTFNFTRGLCMYKDYTARKDFVVSEDAWHSHNYGYPDACPNQLTPMKDFLSL
                     VDGGFAINSPFPLVLQPQRAVDLIVSFDYSLEGPFEVLQVTEKYCRDRGIPFPRIEVD
                     PKDSEDPRECYLFAEAEDPCSPIVLHFPLVNRTFRTHLAPGVERQTAEEKAFGDFIIN
                     GPDTAYGMMDFTYEPKEFDRLVTLSRYNVLNNKETIRHALQLALDRRRQAGGRVGG"
     exon            221..293
                     /gene="Pla2g4f"
                     /gene_synonym="4732472I07Rik; Gm647; Pla2zeta"
                     /inference="alignment:Splign:2.1.0"
     exon            294..430
                     /gene="Pla2g4f"
                     /gene_synonym="4732472I07Rik; Gm647; Pla2zeta"
                     /inference="alignment:Splign:2.1.0"
     exon            431..556
                     /gene="Pla2g4f"
                     /gene_synonym="4732472I07Rik; Gm647; Pla2zeta"
                     /inference="alignment:Splign:2.1.0"
     exon            557..597
                     /gene="Pla2g4f"
                     /gene_synonym="4732472I07Rik; Gm647; Pla2zeta"
                     /inference="alignment:Splign:2.1.0"
     exon            598..640
                     /gene="Pla2g4f"
                     /gene_synonym="4732472I07Rik; Gm647; Pla2zeta"
                     /inference="alignment:Splign:2.1.0"
     exon            641..707
                     /gene="Pla2g4f"
                     /gene_synonym="4732472I07Rik; Gm647; Pla2zeta"
                     /inference="alignment:Splign:2.1.0"
     exon            708..871
                     /gene="Pla2g4f"
                     /gene_synonym="4732472I07Rik; Gm647; Pla2zeta"
                     /inference="alignment:Splign:2.1.0"
     exon            872..985
                     /gene="Pla2g4f"
                     /gene_synonym="4732472I07Rik; Gm647; Pla2zeta"
                     /inference="alignment:Splign:2.1.0"
     exon            986..1023
                     /gene="Pla2g4f"
                     /gene_synonym="4732472I07Rik; Gm647; Pla2zeta"
                     /inference="alignment:Splign:2.1.0"
     exon            1024..1159
                     /gene="Pla2g4f"
                     /gene_synonym="4732472I07Rik; Gm647; Pla2zeta"
                     /inference="alignment:Splign:2.1.0"
     exon            1160..1296
                     /gene="Pla2g4f"
                     /gene_synonym="4732472I07Rik; Gm647; Pla2zeta"
                     /inference="alignment:Splign:2.1.0"
     exon            1297..1519
                     /gene="Pla2g4f"
                     /gene_synonym="4732472I07Rik; Gm647; Pla2zeta"
                     /inference="alignment:Splign:2.1.0"
     exon            1520..1634
                     /gene="Pla2g4f"
                     /gene_synonym="4732472I07Rik; Gm647; Pla2zeta"
                     /inference="alignment:Splign:2.1.0"
     exon            1635..1772
                     /gene="Pla2g4f"
                     /gene_synonym="4732472I07Rik; Gm647; Pla2zeta"
                     /inference="alignment:Splign:2.1.0"
     exon            1773..1880
                     /gene="Pla2g4f"
                     /gene_synonym="4732472I07Rik; Gm647; Pla2zeta"
                     /inference="alignment:Splign:2.1.0"
     exon            1881..2096
                     /gene="Pla2g4f"
                     /gene_synonym="4732472I07Rik; Gm647; Pla2zeta"
                     /inference="alignment:Splign:2.1.0"
     exon            2097..2263
                     /gene="Pla2g4f"
                     /gene_synonym="4732472I07Rik; Gm647; Pla2zeta"
                     /inference="alignment:Splign:2.1.0"
     exon            2264..2450
                     /gene="Pla2g4f"
                     /gene_synonym="4732472I07Rik; Gm647; Pla2zeta"
                     /inference="alignment:Splign:2.1.0"
     exon            2451..3113
                     /gene="Pla2g4f"
                     /gene_synonym="4732472I07Rik; Gm647; Pla2zeta"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3087..3092
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pla2g4f"
                     /gene_synonym="4732472I07Rik; Gm647; Pla2zeta"
                     /note="hexamer: AATAAA"
     polyA_site      3113
                     /gene="Pla2g4f"
                     /gene_synonym="4732472I07Rik; Gm647; Pla2zeta"
                     /note="major polyA site"
ORIGIN      
        1 actcctgtgc tacttgtcag acctgagcga cagtgaatat agccagagaa agggtggctc
       61 tgggaaacag gcaagctccc tactgggacc tgagctgcta ctgctggcca tgccctggac
      121 tctccagcca aagtggctgg caggcaaggg acttcccctt cttggagcca tactgctacg
      181 gaagacagaa aagagcgaac cacaatggaa gcataggcgg caggaaaccc acccatacta
      241 cgaccttcaa gtgaaggtgc tgagggccag aaacatccag cacacagata agttgtccaa
      301 agccgactgc tatgttcgac tgtggctgcc cacggcttct gttagcccca gtcagacaag
      361 gacagtggtt aacagcagtg atccagaatg gaatgagacc tttccctatc agatccacgg
      421 cgctgtgaag aacgtcctgg agcttgccct ttatgacgag gatgtcctgg acagtgacaa
      481 tgtcttctcc attctgtttg acacgagtac tcttcagcta ggccagcctt gcacaaaaaa
      541 cttcaccagg cagcaggatc caaaagagct ggaagtagaa tttactctgg aaaagagtca
      601 gacgcctgca tctgaagttg tcaccaatgg tgtcctggtg gctcacccct gtctgagaat
      661 tcagggcaca gtcactggag acaagacagc ctcccttgga gagttgggct ccaggcagat
      721 ccagctggca gtgcctgggg cctatgaaaa gccacagcct ctgcagccaa cctcggagcc
      781 aggcctccca gtgaacttta ccttccacgt gaacccagtg ctgagcccca agctgcacat
      841 aaagctgcaa gaacagctcc aagtcttcca tagtggcccg agtgatgagc tggaagctca
      901 gaccagcaag atggacaagg caagcatcct gctctcctct ctgcccctca acgaggagtt
      961 aacgaaactt gtggacctgg aggagggcca gcaggtgtct cttaggatga aggcagacat
     1021 gagctctggg gacttggacc tgcgccttgg ttttgacctc tgtgatggag agcaggaatt
     1081 tctggacaag aggaagcagg tggcgtccaa ggccctgcag cgggtgatgg gattgagtga
     1141 ggctctgcac tgtgaccagg tacctgtggt agccgtgtta ggctctgggg gtggaaccag
     1201 agccatgact tccctgtacg gcagcctggc tgggctgcag gagcttggtc ttctggatgc
     1261 cgtgacctac ctgagtgggg tctctgggtc ttcctggtgc atctctacac tctacaggga
     1321 tccatcctgg tcccagaagg ctttgcaggg ccccattaaa tatgcctcag agcgagtctg
     1381 cagcagtaaa attgggatgc tgtccccaaa gcagtttgaa tactactccc gggaaaagag
     1441 agcctgggag agcaggggac acagcatgtc cttcactgac ttgtggggcc tcatcattga
     1501 gtatttcctg aaccaggagg aaaaccctgc caagctgtca gaccagcaag aaacggtcag
     1561 ccagggtcag aacccatacc ccatctatgc cagcattaat gtccacaaaa acatcagtgg
     1621 ggacgacttt gcagagtggt gcgagttcac cccctatgag gtcggtttcc ccaagtacgg
     1681 ggcttacgtt cccacggaac tctttggctc tgaattcttc atgggccggc tgctgcattt
     1741 ctggccagag ccccgcatct gttacctgca gggtatgtgg ggaagtgctt ttgcagccag
     1801 cctgtatgag atcttcctga agctgggagg cctaagcctg agctttctgg actggcacag
     1861 ggggagtgtc agtgtcacag atgactggcc aaagttacgg aagcaggacc ccacacggct
     1921 gcctaccaga ctcttcacgc caatgagttc cttctctcag gctgtgctgg acatattcac
     1981 ctcccgtatt acttgtgccc agacctttaa ctttacccga ggtctctgca tgtacaaaga
     2041 ctacacagct agaaaggact tcgtggtctc tgaagatgca tggcattcac ataactatgg
     2101 ataccctgat gcctgtccca accagctcac acccatgaag gacttcctgt ccctagtaga
     2161 tggaggcttt gctatcaact cgccatttcc actggtcctg cagccgcagc gggctgtgga
     2221 cctcattgtg tcctttgact attccttgga aggccctttt gaggtcctgc aggtgacaga
     2281 gaagtactgc cgggaccgag ggatcccctt cccaaggatt gaggtggacc ccaaggactc
     2341 tgaagacccc cgtgaatgct atctgtttgc tgaggcagag gacccctgct cgcccatcgt
     2401 gctgcatttc cctcttgtca acaggacctt tcgcacgcac ctggccccag gtgtggaacg
     2461 acaaacagct gaggagaagg ccttcgggga ctttatcatc aacgggccag atactgccta
     2521 tggcatgatg gatttcacct acgagcccaa ggaatttgat cggctggtga ccctgagccg
     2581 atacaacgtc ttgaacaaca aggagactat caggcatgcc ctccagctgg ctctggaccg
     2641 gcggcggcag gctgggggaa gggttggggg ctgatcacat gagagtcaga ggactgtggt
     2701 ggtgtgatgg aggaccttaa gtcagagtat gctgagggag agggaagact ttaaacactt
     2761 tctgttttcc acttctcctt cccagagaag atggggcagt atctctctct ctctctctga
     2821 gtgcttgggg gtcctgtgca ggagagaaca gagttcatat tatattgggg tgtagagagc
     2881 caggcagcag cttcatcaga aggcgcaccc ccacccccac cacagaagga cctctggaaa
     2941 gaacccaagc attcagagct tcaccacaga gctgtgggct gaggaaccag ctgtccttac
     3001 actgatgcag aactacagct gctcacactt ccacagagtg gcccagctct gacccactcc
     3061 aaacccccgg actcagtgat gtggagaata aacagcagct atgtgggtcg cca
//
