LOCUS       NM_001395470            4599 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus phospholipase A2, group IVE (Pla2g4e), transcript
            variant 4, mRNA.
ACCESSION   NM_001395470 XM_006499771
VERSION     NM_001395470.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4599)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4599)
  AUTHORS   Rahman,S.M.K., Hussain,Z., Morito,K., Takahashi,N., Sikder,M.M.,
            Tanaka,T., Ohta,K.I., Ueno,M., Takahashi,H., Yamamoto,T.,
            Murakami,M., Uyama,T. and Ueda,N.
  TITLE     Formation of N-acyl-phosphatidylethanolamines by cytosolic
            phospholipase A2epsilon in an ex vivo murine model of brain
            ischemia
  JOURNAL   Biochim Biophys Acta Mol Cell Biol Lipids 1867 (12), 159222 (2022)
   PUBMED   35988872
REFERENCE   3  (bases 1 to 4599)
  AUTHORS   Liang,L., Takamiya,R., Miki,Y., Heike,K., Taketomi,Y., Sugimoto,N.,
            Yamaguchi,M., Shitara,H., Nishito,Y., Kobayashi,T., Hirabayashi,T.
            and Murakami,M.
  TITLE     Group IVE cytosolic phospholipase A2 limits psoriatic inflammation
            by mobilizing the anti-inflammatory lipid N-acylethanolamine
  JOURNAL   FASEB J 36 (5), e22301 (2022)
   PUBMED   35478358
REFERENCE   4  (bases 1 to 4599)
  AUTHORS   Hussain,Z., Uyama,T., Kawai,K., Binte Mustafiz,S.S., Tsuboi,K.,
            Araki,N. and Ueda,N.
  TITLE     Phosphatidylserine-stimulated production of
            N-acyl-phosphatidylethanolamines by Ca2+-dependent
            N-acyltransferase
  JOURNAL   Biochim Biophys Acta Mol Cell Biol Lipids 1863 (5), 493-502 (2018)
   PUBMED   29447909
REFERENCE   5  (bases 1 to 4599)
  AUTHORS   Ogura,Y., Parsons,W.H., Kamat,S.S. and Cravatt,B.F.
  TITLE     A calcium-dependent acyltransferase that produces N-acyl
            phosphatidylethanolamines
  JOURNAL   Nat Chem Biol 12 (9), 669-671 (2016)
   PUBMED   27399000
REFERENCE   6  (bases 1 to 4599)
  AUTHORS   Capestrano,M., Mariggio,S., Perinetti,G., Egorova,A.V.,
            Iacobacci,S., Santoro,M., Di Pentima,A., Iurisci,C., Egorov,M.V.,
            Di Tullio,G., Buccione,R., Luini,A. and Polishchuk,R.S.
  TITLE     Cytosolic phospholipase Aepsilon drives recycling through the
            clathrin-independent endocytic route
  JOURNAL   J Cell Sci 127 (Pt 5), 977-993 (2014)
   PUBMED   24413173
REFERENCE   7  (bases 1 to 4599)
  AUTHORS   Ohto,T., Uozumi,N., Hirabayashi,T. and Shimizu,T.
  TITLE     Identification of novel cytosolic phospholipase A(2)s, murine
            cPLA(2){delta}, {epsilon}, and {zeta}, which form a gene cluster
            with cPLA(2){beta}
  JOURNAL   J Biol Chem 280 (26), 24576-24583 (2005)
   PUBMED   15866882
  REMARK    GeneRIF: murine cytosolic phospholipases A2 delta, epsilon, and
            zeta form a gene cluster with cPLA(2)beta
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL844608.18, BX000525.8 and AL833774.4.
            
            On Jul 8, 2021 this sequence version replaced XM_006499771.5.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR9219379.41140.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-555               AL844608.18        44619-45173         c
            556-666             AL844608.18        9746-9856           c
            667-772             AL844608.18        7063-7168           c
            773-845             BX000525.8         14183-14255         c
            846-982             BX000525.8         5164-5300           c
            983-1114            BX000525.8         3374-3505           c
            1115-1155           BX000525.8         2480-2520           c
            1156-1198           AL833774.4         793-835
            1199-1262           AL833774.4         1621-1684
            1263-1423           AL833774.4         2682-2842
            1424-1510           AL833774.4         2945-3031
            1511-1548           AL833774.4         3423-3460
            1549-1684           AL833774.4         5192-5327
            1685-1821           AL833774.4         6478-6614
            1822-2044           AL833774.4         11087-11309
            2045-2159           AL833774.4         13664-13778
            2160-2297           AL833774.4         14241-14378
            2298-2405           AL833774.4         16206-16313
            2406-2609           AL833774.4         16720-16923
            2610-2776           AL833774.4         17836-18002
            2777-2960           AL833774.4         19376-19559
            2961-4599           AL833774.4         19981-21619
FEATURES             Location/Qualifiers
     source          1..4599
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 60.24 cM"
     gene            1..4599
                     /gene="Pla2g4e"
                     /gene_synonym="2310026J01Rik; C230096D22; Pla2epsilon"
                     /note="phospholipase A2, group IVE"
                     /db_xref="GeneID:329502"
                     /db_xref="MGI:MGI:1919144"
     exon            1..555
                     /gene="Pla2g4e"
                     /gene_synonym="2310026J01Rik; C230096D22; Pla2epsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            556..666
                     /gene="Pla2g4e"
                     /gene_synonym="2310026J01Rik; C230096D22; Pla2epsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            667..772
                     /gene="Pla2g4e"
                     /gene_synonym="2310026J01Rik; C230096D22; Pla2epsilon"
                     /inference="alignment:Splign:2.1.0"
     CDS             683..3178
                     /gene="Pla2g4e"
                     /gene_synonym="2310026J01Rik; C230096D22; Pla2epsilon"
                     /EC_number="3.1.1.4"
                     /note="isoform 4 is encoded by transcript variant 4;
                     cytosolic phospholipase A2 epsilon; phospholipase A2,
                     group 4E; cPLA2-epsilon; calcium-dependent
                     N-acyltransferase"
                     /codon_start=1
                     /product="cytosolic phospholipase A2 epsilon isoform 4"
                     /protein_id="NP_001382399.1"
                     /db_xref="GeneID:329502"
                     /db_xref="MGI:MGI:1919144"
                     /translation="MLQRQMPAWVSHSLSDPCQAEDGMASGALKEGSSPCHLLTVRII
                     GMKNVRQADILSQTDCFVTLWLPTASQKKLKTRTISNCLHPEWDESFTFQIQTQVKNV
                     LELSVCDEDTLTQNDHLLTVLYDLSKLCLRNKTHVKFPLNPEGMEELEVEFLLEENFS
                     SSETLITNGVLVSRQVSCLEVHAESRRPRKRKKNKDLLVMVTDSFENTQRVPPCQEPC
                     YPNSACFHYPKYSQPQLYAEAPKSHCNFRLCCCGTHRNDPVCQPLNCLSDGQVTTLPV
                     GENYELHMKSSPCSDTLDVRLGFSLCQEEVEFVQKRKMVVAKTLSQMLQLEEGLHEDE
                     VPIIAIMATGGGTRSMVSLYGHLLGLQKLNFLDASTYITGLSGATWTMATLYSDPEWS
                     SKNLETVVFEARRHVVKDKMPALFPDQLYKWREDLQKHSQEGYKTTFTDFWGKLIEYS
                     LGDKKNECKLSDQRAALCRGQNPLPIYLTINVKDDVSNQDFREWFEFSPYEVGMQKYG
                     AFIPSELFGSEFFMGRLMKRIPEPEMCYMLGLWSSIFSLNLLDAWNLSHTSEEFFYRW
                     TRERLHDIEDDPILPEIPRCDDNPLETTVVIPTTWLSNTFREILTRRPFVSEFHNFLY
                     GMQLHTDYLQNRQFSMWKDTVLDTFPNQLTQFAKHLNLLDTAFFVNSSYAPLLRPERK
                     VDLIIHLNYCAGSQTKPLKQTCEYCTEQKIPFPSFSILEDDNSLKECYVMENPQEPDA
                     PIVAYFPLISDTFQKYKAPGVERSPDELELGQLNIYGPKSPYATKELTYTEAAFDKLV
                     KLSEYNILNNRDKLIQALRLAMEKKRMRSQCPS"
     exon            773..845
                     /gene="Pla2g4e"
                     /gene_synonym="2310026J01Rik; C230096D22; Pla2epsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            846..982
                     /gene="Pla2g4e"
                     /gene_synonym="2310026J01Rik; C230096D22; Pla2epsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            983..1114
                     /gene="Pla2g4e"
                     /gene_synonym="2310026J01Rik; C230096D22; Pla2epsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            1115..1155
                     /gene="Pla2g4e"
                     /gene_synonym="2310026J01Rik; C230096D22; Pla2epsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            1156..1198
                     /gene="Pla2g4e"
                     /gene_synonym="2310026J01Rik; C230096D22; Pla2epsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            1199..1262
                     /gene="Pla2g4e"
                     /gene_synonym="2310026J01Rik; C230096D22; Pla2epsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            1263..1423
                     /gene="Pla2g4e"
                     /gene_synonym="2310026J01Rik; C230096D22; Pla2epsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            1424..1510
                     /gene="Pla2g4e"
                     /gene_synonym="2310026J01Rik; C230096D22; Pla2epsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            1511..1548
                     /gene="Pla2g4e"
                     /gene_synonym="2310026J01Rik; C230096D22; Pla2epsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            1549..1684
                     /gene="Pla2g4e"
                     /gene_synonym="2310026J01Rik; C230096D22; Pla2epsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            1685..1821
                     /gene="Pla2g4e"
                     /gene_synonym="2310026J01Rik; C230096D22; Pla2epsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            1822..2044
                     /gene="Pla2g4e"
                     /gene_synonym="2310026J01Rik; C230096D22; Pla2epsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            2045..2159
                     /gene="Pla2g4e"
                     /gene_synonym="2310026J01Rik; C230096D22; Pla2epsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            2160..2297
                     /gene="Pla2g4e"
                     /gene_synonym="2310026J01Rik; C230096D22; Pla2epsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            2298..2405
                     /gene="Pla2g4e"
                     /gene_synonym="2310026J01Rik; C230096D22; Pla2epsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            2406..2609
                     /gene="Pla2g4e"
                     /gene_synonym="2310026J01Rik; C230096D22; Pla2epsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            2610..2776
                     /gene="Pla2g4e"
                     /gene_synonym="2310026J01Rik; C230096D22; Pla2epsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            2777..2960
                     /gene="Pla2g4e"
                     /gene_synonym="2310026J01Rik; C230096D22; Pla2epsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            2961..4599
                     /gene="Pla2g4e"
                     /gene_synonym="2310026J01Rik; C230096D22; Pla2epsilon"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4577..4582
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pla2g4e"
                     /gene_synonym="2310026J01Rik; C230096D22; Pla2epsilon"
                     /note="hexamer: AATAAA"
     polyA_site      4599
                     /gene="Pla2g4e"
                     /gene_synonym="2310026J01Rik; C230096D22; Pla2epsilon"
                     /note="major polyA site"
ORIGIN      
        1 cttgcagtgc agagctggag acagccccga gcctgaatcg cagctctgca aagccggtct
       61 ctcctcgtct agcactacgt agtgcggggc ggcggcggcg gcgcagtcct tgttgcgtgc
      121 tgaccctctg cacaccacgg aacctttctt ttactcttgc caaccaggtt gctcgaaatg
      181 tttccggcgc tcccttagcg aagctgtgac ctctttccct ccctccctcc ttaaaaattt
      241 atctgacatt tctcctggag gaacctgctg acacctttcc tttctcccaa acctctcctg
      301 aacttccctg aggataaaat cggctccagc tctatgcagt ctattccaca ctccgatgaa
      361 gcagacgtgg ctgggatgac ccacgcctca gaaggccacc atggcctggg gaccagcatg
      421 cttgtcccaa agaacccaca aggggaagaa gacagcaagc taggaagaaa ctgcagtgga
      481 tttgaagatg cacaggaccc acagactgct gtgccctcct cacctttact ttccatggct
      541 tcttgcagtt ctcagagtcc tcagaagaga caactggctt gaactcagcc ccttccttct
      601 gtgatgactt cttgtctcat tcaagaacct tcctgggcag tcagctcaga agcaggcaca
      661 tagcagctgg agagccgcca cgatgcttca gaggcagatg cctgcttggg tttcccactc
      721 cctgtcagac ccatgccaag cagaagatgg catggcctcc ggtgccttga aggaggggtc
      781 atctccatgc catctgttga cagtgaggat cattggcatg aaaaacgtcc ggcaggctga
      841 tatactgagt cagacagact gctttgtgac cctctggctg cctactgcct ctcagaagaa
      901 gctgaagacc agaaccatct ccaactgcct acacccagag tgggacgaaa gcttcacctt
      961 tcagatccag actcaagtaa agaatgtgct agagctgagc gtctgtgacg aagacaccct
     1021 gacacaaaat gaccatctct tgacagtcct ctatgacctc tctaagcttt gcctccggaa
     1081 taaaacccat gtgaagttcc cactcaaccc agagggcatg gaagaactgg aggtggagtt
     1141 cctactcgaa gagaatttct cctcatcaga gaccctcatc accaacggcg tgctggtgtc
     1201 tcgccaagtc tcttgcctgg aggttcatgc agaatccagg aggccgagga agaggaagaa
     1261 aaacaaagac cttctggtga tggtgacaga ctccttcgag aacacccagc gtgtcccgcc
     1321 ttgccaggag ccctgctacc ccaattctgc ctgcttccac taccccaagt actcccagcc
     1381 acagctttac gcagaggcgc ctaagagcca ctgtaacttt aggctttgct gctgcggaac
     1441 acacaggaat gaccctgtct gccagcccct caattgcctt tctgatggcc aggtgacaac
     1501 cctgcctgtg ggagagaact atgagctaca catgaagtcc tcaccctgct ctgacacact
     1561 ggatgtgcgg cttggattca gcctgtgcca ggaagaggtg gagtttgtgc agaagcggaa
     1621 gatggtggtg gccaagacac taagtcagat gctgcagctg gaggaaggcc tgcatgagga
     1681 tgaggtaccg ataatagcca tcatggccac aggaggtggc acaaggtcta tggtctcctt
     1741 gtatggccac ctgctggggt tgcagaagct gaactttctg gacgcttcta cttacatcac
     1801 cggcttgtca ggtgcaacct ggactatggc taccttgtac agtgatcctg agtggtcctc
     1861 caaaaacctg gagactgttg tctttgaggc ccggagacat gttgtcaaag acaagatgcc
     1921 tgccctgttc ccagatcagc tctacaaatg gcgagaggac ctccaaaagc atagccagga
     1981 gggctataag accacgttta cagacttttg gggcaagctg atcgagtaca gtctgggaga
     2041 taaaaaaaac gaatgcaagc tgtcagatca gcgagctgct ctgtgcaggg gacagaaccc
     2101 tctgcccatc tacctcacca tcaatgtcaa ggatgatgta agcaaccagg atttcagaga
     2161 atggttcgag ttctccccct acgaggtggg catgcagaag tacggagcct tcatccccag
     2221 cgagttattt ggctccgagt tcttcatggg gcggctgatg aagaggattc ctgagccgga
     2281 gatgtgctac atgctagggt tgtggagtag catcttttcc ctgaacctgc ttgatgcctg
     2341 gaatttgtct cacacctcag aggagttttt ctataggtgg acaagggaga gactgcatga
     2401 catcgaagat gatcccatcc tgcctgaaat ccctaggtgt gacgataacc ccctagagac
     2461 cacagtagtg atcccaacga catggctgtc caacaccttc cgagaaatcc tcacacgcag
     2521 gcccttcgtg tctgagttcc acaacttcct gtacgggatg cagctgcata ctgactactt
     2581 acagaacagg cagttctcta tgtggaaaga cacagtactg gacaccttcc caaaccagct
     2641 gacacagttt gcaaaacacc tgaacctgct ggacactgcg ttctttgtca actccagcta
     2701 cgcacccctc cttaggccag agagaaaagt cgaccttatc atccacctca attactgcgc
     2761 aggatcccag acaaagcccc tgaaacaaac ctgtgagtac tgtaccgagc agaagatccc
     2821 cttccccagc ttctccatcc tggaagatga caacagtctc aaggagtgct acgtgatgga
     2881 gaatccccag gagcccgacg cccccatcgt ggcttacttc ccactcatca gtgacacctt
     2941 ccagaagtac aaggctccag gtgtagagcg aagtcctgac gagctggaac tgggccagct
     3001 gaacatctat ggaccaaagt ctccctatgc caccaaggag ctgacgtaca cagaggccgc
     3061 cttcgacaag ctggtgaagc tctcagaata taatatcctc aataacagag ataagctcat
     3121 tcaggccttg agactagcaa tggagaagaa acgcatgagg agccagtgtc cctcctaggc
     3181 ctgggggagt cctatctatc tcgtgtctgc ttctattgtc agaagcatcg cccccaaagc
     3241 agacctcaca cacagctgtc cctgatctct ggctagggtc gcaacacctt ggcctgggct
     3301 ttctagtaaa atgttacaaa tgaacaaagg tgagagagaa aagaagagtc tattgggttt
     3361 tatacccctg gggcttctct ccactttctg gcaaggaagg taccagggtc acagactccc
     3421 acacagtctc aagtatagaa tgtgaatatg tgggcacaga gagtggctag ttcccaagag
     3481 ccctcaccct tcgagctgga ggcaaaagtt ccctaagcat cccctcccct ccttttcctc
     3541 cttccatggc cagtctagag aaatgtccag gaagaccaag aacagcagaa gctcacagag
     3601 tgaggatcca gggaagcctt ggcaggctgt gggcaagcgg caccgggtgc atgacgctat
     3661 ctggcgccag taacgtcctc tggcatctct ctggcttgca gatggggtta tttatttata
     3721 agtatgattc ttcttattta tcacagaaac actatggccg atttatctac gttcttatac
     3781 agtgactcag agaataaggt taggaaagat gacctcagaa tggtgagtta ggactctgac
     3841 aaagacctca gctttggagg agaatctctg gggaagccct ggacacccgg acacccagcc
     3901 tcctggcttc actaaaagat agaagggggt ccctgcagtg agcaggtaaa agccaaaggg
     3961 cagctaggta tgtggccttt ggggagccca acgtgcctgg aggacaaggg cctctttctg
     4021 agccacatga tccctccaca gtcctggttg ttgagagatt aatgacaccc aacggtgttg
     4081 tttatatgtt tcaaatggac tcctggttcc atcattgcta tttaaagtgg attcgattgg
     4141 gcctggctat aagcccagac ccagccacac ctacaggaac ccatactgtg aagagacacc
     4201 agtgcctctg ctccagcctt acctgtcagt gaggggggtt gactcctgtg tgggtaccat
     4261 tggggaagga tggatttatc acactctcct accagcctgg ggagcatgag tccatagact
     4321 ctgctgagcc actgataagc tgggcttctg agcctggaaa actggtccct acccaagtgt
     4381 tgctgatgtg agttggatgt tcatcacctc ctctagccct ccactggagt agctggctgg
     4441 cattgctttt gcttccccca tgtagagaac aggctctgaa ttgtcaaaga gagccaagat
     4501 agaaccttgt attttgcaag gtttgactca ccatagcata taaagtttat aagagcactg
     4561 taccatttga acacagaata aagtggttat gaaggtgaa
//
