LOCUS       NM_008869               2869 bp    mRNA    linear   ROD 12-FEB-2025
DEFINITION  Mus musculus phospholipase A2, group IVA (cytosolic,
            calcium-dependent) (Pla2g4a), transcript variant 1, mRNA.
ACCESSION   NM_008869
VERSION     NM_008869.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Glaser,E.P., Kopper,T.J., Bailey,W.M., Kashif,H.K., Kumari,R.,
            Stewart,A.N. and Gensel,J.C.
  TITLE     Cytosolic phospholipase A2 in infiltrating monocyte derived
            macrophages does not impair recovery after spinal cord injury in
            female mice
  JOURNAL   Sci Rep 15 (1), 1 (2025)
   PUBMED   39747330
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Taketomi,Y., Higashi,T., Kano,K., Miki,Y., Mochizuki,C.,
            Toyoshima,S., Okayama,Y., Nishito,Y., Nakae,S., Tanaka,S.,
            Tokuoka,S.M., Oda,Y., Shichino,S., Ueha,S., Matsushima,K.,
            Akahoshi,N., Ishii,S., Chun,J., Aoki,J. and Murakami,M.
  TITLE     Lipid-orchestrated paracrine circuit coordinates mast cell
            maturation and anaphylaxis through functional interaction with
            fibroblasts
  JOURNAL   Immunity 57 (8), 1828-1847 (2024)
   PUBMED   39002541
REFERENCE   3  (bases 1 to 2869)
  AUTHORS   Kishinaka,S., Kawashita,E., Nishizaki,T., Ishihara,K. and Akiba,S.
  TITLE     Group IVA Phospholipase A2 in Collagen-Producing Cells Promotes
            High-Fat Diet-Induced Infiltration of Inflammatory Cells into the
            Liver by Upregulating the Expression of MCP-1
  JOURNAL   Biol Pharm Bull 47 (5), 1058-1065 (2024)
   PUBMED   38825533
  REMARK    GeneRIF: Group IVA Phospholipase A2 in Collagen-Producing Cells
            Promotes High-Fat Diet-Induced Infiltration of Inflammatory Cells
            into the Liver by Upregulating the Expression of MCP-1.
REFERENCE   4  (bases 1 to 2869)
  AUTHORS   Nakamura,A., Sakai,S., Taketomi,Y., Tsuyama,J., Miki,Y., Hara,Y.,
            Arai,N., Sugiura,Y., Kawaji,H., Murakami,M. and Shichita,T.
  TITLE     PLA2G2E-mediated lipid metabolism triggers brain-autonomous neural
            repair after ischemic stroke
  JOURNAL   Neuron 111 (19), 2995-3010 (2023)
   PUBMED   37490917
REFERENCE   5  (bases 1 to 2869)
  AUTHORS   Bonventre,J.V.
  TITLE     The 85-kD cytosolic phospholipase A2 knockout mouse: a new tool for
            physiology and cell biology
  JOURNAL   J Am Soc Nephrol 10 (2), 404-412 (1999)
   PUBMED   10215342
  REMARK    Review article
REFERENCE   6  (bases 1 to 2869)
  AUTHORS   Klivenyi,P., Beal,M.F., Ferrante,R.J., Andreassen,O.A., Wermer,M.,
            Chin,M.R. and Bonventre,J.V.
  TITLE     Mice deficient in group IV cytosolic phospholipase A2 are resistant
            to MPTP neurotoxicity
  JOURNAL   J Neurochem 71 (6), 2634-2637 (1998)
   PUBMED   9832165
REFERENCE   7  (bases 1 to 2869)
  AUTHORS   Bonventre,J.V., Huang,Z., Taheri,M.R., O'Leary,E., Li,E.,
            Moskowitz,M.A. and Sapirstein,A.
  TITLE     Reduced fertility and postischaemic brain injury in mice deficient
            in cytosolic phospholipase A2
  JOURNAL   Nature 390 (6660), 622-625 (1997)
   PUBMED   9403693
REFERENCE   8  (bases 1 to 2869)
  AUTHORS   Uozumi,N., Kume,K., Nagase,T., Nakatani,N., Ishii,S., Tashiro,F.,
            Komagata,Y., Maki,K., Ikuta,K., Ouchi,Y., Miyazaki,J. and
            Shimizu,T.
  TITLE     Role of cytosolic phospholipase A2 in allergic response and
            parturition
  JOURNAL   Nature 390 (6660), 618-622 (1997)
   PUBMED   9403692
REFERENCE   9  (bases 1 to 2869)
  AUTHORS   Clark,M.A., Ozgur,L.E., Conway,T.M., Dispoto,J., Crooke,S.T. and
            Bomalaski,J.S.
  TITLE     Cloning of a phospholipase A2-activating protein
  JOURNAL   Proc Natl Acad Sci U S A 88 (12), 5418-5422 (1991)
   PUBMED   2052621
REFERENCE   10 (bases 1 to 2869)
  AUTHORS   Clark,J.D., Lin,L.L., Kriz,R.W., Ramesha,C.S., Sultzman,L.A.,
            Lin,A.Y., Milona,N. and Knopf,J.L.
  TITLE     A novel arachidonic acid-selective cytosolic PLA2 contains a
            Ca(2+)-dependent translocation domain with homology to PKC and GAP
  JOURNAL   Cell 65 (6), 1043-1051 (1991)
   PUBMED   1904318
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC158151.4, M72394.1 and
            BQ033204.1.
            
            On Mar 24, 2015 this sequence version replaced NM_008869.3.
            
            Summary: The protein encoded by this gene is a member of the
            phospholipase A2 group IV family. This enzyme hydrolyzes membrane
            phospholipids, thereby releasing the polyunsaturated fatty acid,
            arachidonic acid. Arachidonic acid is further metabolized into
            eicosanoids such as leukotrienes, thromboxanes and prostaglandins,
            that play important roles in regulating diverse biological
            processes such as inflammatory responses, membrane and actin
            dynamics, and tumorigenesis. A rise in intracellular calcium levels
            results in binding of calcium to the C2 domain of this protein, and
            triggers the translocation from the cytosol to intracellular
            membranes, including the Golgi apparatus. Disruption of this gene
            in mice led to decreased levels of eicosonaoids and
            platelet-activating factor, decreased allergic symptoms, and
            impaired reproductive ability in females. Alternative splicing
            results in multiple transcript variants encoding different
            isoforms. [provided by RefSeq, Mar 2015].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M72394.1, BC003816.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-58                AC158151.4         72753-72810
            59-2845             M72394.1           1-2787
            2846-2869           BQ033204.1         1-24                c
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 63.51 cM"
     gene            1..2869
                     /gene="Pla2g4a"
                     /gene_synonym="cPLA2; cPLA2-alpha; cPLA2alpha; Pla2g4"
                     /note="phospholipase A2, group IVA (cytosolic,
                     calcium-dependent)"
                     /db_xref="GeneID:18783"
                     /db_xref="MGI:MGI:1195256"
     exon            1..100
                     /gene="Pla2g4a"
                     /gene_synonym="cPLA2; cPLA2-alpha; cPLA2alpha; Pla2g4"
                     /inference="alignment:Splign:2.1.0"
     exon            101..199
                     /gene="Pla2g4a"
                     /gene_synonym="cPLA2; cPLA2-alpha; cPLA2alpha; Pla2g4"
                     /inference="alignment:Splign:2.1.0"
     CDS             167..2413
                     /gene="Pla2g4a"
                     /gene_synonym="cPLA2; cPLA2-alpha; cPLA2alpha; Pla2g4"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.1.4"
                     /note="isoform 1 is encoded by transcript variant 1;
                     phospholipase A2 group IVA; phospholipase A2, group 4;
                     Type IV PLA2; cytosolic PLA2; Cytosolic phospholipase A2
                     (CPLA2)"
                     /codon_start=1
                     /product="cytosolic phospholipase A2 isoform 1"
                     /protein_id="NP_032895.1"
                     /db_xref="CCDS:CCDS15352.1"
                     /db_xref="GeneID:18783"
                     /db_xref="MGI:MGI:1195256"
                     /translation="MSFIDPYQHIIVEHQYSHKFTVVVLRATKVTKGTFGDMLDTPDP
                     YVELFISTTPDSRKRTRHFNNDINPVWNETFEFILDPNQENVLEITLMDANYVMDETL
                     GTATFPVSSMKVGEKKEVPFIFNQVTEMILEMSLEVCSCPDLRFSMALCDQEKTFRQQ
                     RKENIKENMKKLLGPKKSEGLYSTRDVPVVAILGSGGGFRAMVGFSGVMKALYESGIL
                     DCATYIAGLSGSTWYMSTLYSHPDFPEKGPEEINEELMKNVSHNPLLLLTPQKVKRYV
                     ESLWKKKSSGQPVTFTDIFGMLIGETLIQNRMSMTLSSLKEKVNAARCPLPLFTCLHV
                     KPDVSELMFADWVEFSPYEIGMAKYGTFMAPDLFGSKFFMGTVVKKYEENPLHFLMGV
                     WGSAFSILFNRVLGVSGSQNKGSTMEEELENITAKHIVSNDSSDSDDEAQGPKGTENE
                     EAEKEYQSDNQASWVHRMLMALVSDSALFNTREGRAGKVHNFMLGLNLNTSYPLSPLR
                     DFSSQDSFDDELDAAVADPDEFERIYEPLDVKSKKIHVVDSGLTFNLPYPLILRPQRG
                     VDLIISFDFSARPSDTSPPFKELLLAEKWAKMNKLPFPKIDPYVFDREGLKECYVFKP
                     KNPDVEKDCPTIIHFVLANINFRKYKAPGVLRETKEEKEIADFDIFDDPESPFSTFNF
                     QYPNQAFKRLHDLMYFNTLNNIDVIKDAIVESIEYRRQNPSRCSVSLSNVEARKFFNK
                     EFLSKPTV"
     exon            200..281
                     /gene="Pla2g4a"
                     /gene_synonym="cPLA2; cPLA2-alpha; cPLA2alpha; Pla2g4"
                     /inference="alignment:Splign:2.1.0"
     exon            282..430
                     /gene="Pla2g4a"
                     /gene_synonym="cPLA2; cPLA2-alpha; cPLA2alpha; Pla2g4"
                     /inference="alignment:Splign:2.1.0"
     exon            431..544
                     /gene="Pla2g4a"
                     /gene_synonym="cPLA2; cPLA2-alpha; cPLA2alpha; Pla2g4"
                     /inference="alignment:Splign:2.1.0"
     exon            545..582
                     /gene="Pla2g4a"
                     /gene_synonym="cPLA2; cPLA2-alpha; cPLA2alpha; Pla2g4"
                     /inference="alignment:Splign:2.1.0"
     exon            583..724
                     /gene="Pla2g4a"
                     /gene_synonym="cPLA2; cPLA2-alpha; cPLA2alpha; Pla2g4"
                     /inference="alignment:Splign:2.1.0"
     exon            725..861
                     /gene="Pla2g4a"
                     /gene_synonym="cPLA2; cPLA2-alpha; cPLA2alpha; Pla2g4"
                     /inference="alignment:Splign:2.1.0"
     exon            862..1084
                     /gene="Pla2g4a"
                     /gene_synonym="cPLA2; cPLA2-alpha; cPLA2alpha; Pla2g4"
                     /inference="alignment:Splign:2.1.0"
     exon            1085..1199
                     /gene="Pla2g4a"
                     /gene_synonym="cPLA2; cPLA2-alpha; cPLA2alpha; Pla2g4"
                     /inference="alignment:Splign:2.1.0"
     exon            1200..1337
                     /gene="Pla2g4a"
                     /gene_synonym="cPLA2; cPLA2-alpha; cPLA2alpha; Pla2g4"
                     /inference="alignment:Splign:2.1.0"
     exon            1338..1430
                     /gene="Pla2g4a"
                     /gene_synonym="cPLA2; cPLA2-alpha; cPLA2alpha; Pla2g4"
                     /inference="alignment:Splign:2.1.0"
     exon            1431..1502
                     /gene="Pla2g4a"
                     /gene_synonym="cPLA2; cPLA2-alpha; cPLA2alpha; Pla2g4"
                     /inference="alignment:Splign:2.1.0"
     exon            1503..1742
                     /gene="Pla2g4a"
                     /gene_synonym="cPLA2; cPLA2-alpha; cPLA2alpha; Pla2g4"
                     /inference="alignment:Splign:2.1.0"
     exon            1743..1927
                     /gene="Pla2g4a"
                     /gene_synonym="cPLA2; cPLA2-alpha; cPLA2alpha; Pla2g4"
                     /inference="alignment:Splign:2.1.0"
     exon            1928..2123
                     /gene="Pla2g4a"
                     /gene_synonym="cPLA2; cPLA2-alpha; cPLA2alpha; Pla2g4"
                     /inference="alignment:Splign:2.1.0"
     exon            2124..2281
                     /gene="Pla2g4a"
                     /gene_synonym="cPLA2; cPLA2-alpha; cPLA2alpha; Pla2g4"
                     /inference="alignment:Splign:2.1.0"
     exon            2282..2856
                     /gene="Pla2g4a"
                     /gene_synonym="cPLA2; cPLA2-alpha; cPLA2alpha; Pla2g4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2822..2827
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pla2g4a"
                     /gene_synonym="cPLA2; cPLA2-alpha; cPLA2alpha; Pla2g4"
                     /note="hexamer: AATAAA"
     polyA_site      2852
                     /gene="Pla2g4a"
                     /gene_synonym="cPLA2; cPLA2-alpha; cPLA2alpha; Pla2g4"
                     /note="major polyA site"
ORIGIN      
        1 gccggagaag actttctaga agtcggaact gtgaagggct cccgactgag agccagaagg
       61 cacagagaag cctgaggatt ctcatttaac tctgggaact gcttcaagaa gctacagtac
      121 catagaagac ctgggaagtg tgagaatttc tgcaactggg accaaaatgt ctttcataga
      181 tccttatcag cacattatag tggaacacca gtactcccat aagtttactg ttgtggttct
      241 acgtgccacc aaagtaacca aggggacctt tggcgatatg ctggacactc cagatcctta
      301 tgtggaactt ttcatctcta caacccctga cagcaggaag cgaacgagac acttcaataa
      361 tgatataaac cccgtgtgga atgagacctt tgagttcatt ttggatccta atcaggaaaa
      421 tgttttggag atcacactga tggatgccaa ctacgtcatg gatgaaaccc taggcacagc
      481 tacattccct gtatcttcaa tgaaagtggg agagaagaaa gaagtccctt ttattttcaa
      541 ccaagtcact gaaatgattc tggaaatgtc tctggaagtt tgttcatgcc cagacctacg
      601 gttcagcatg gcactgtgtg atcaggagaa aactttcaga cagcagagga aagagaacat
      661 aaaagagaac atgaagaaac ttttgggtcc aaaaaagagt gaggggcttt attccacacg
      721 tgatgtgccg gtggtggcca ttttgggttc aggtgggggt ttccgggcca tggtgggatt
      781 ctctggtgtg atgaaggcac tgtatgagtc ggggattttg gactgtgcta catacattgc
      841 tggtctttct ggatccacat ggtacatgtc aaccttgtac tctcaccccg attttccaga
      901 gaaaggtccc gaggagatta atgaagagct aatgaaaaat gtcagccaca accctctctt
      961 acttcttaca ccacagaaag ttaaaagata cgttgagtct ttatggaaga agaaaagttc
     1021 tggccagcct gtcaccttta ctgacatctt tgggatgcta ataggagaaa cactaattca
     1081 aaataggatg agcatgaccc tgagtagttt gaaggaaaag gtcaatgccg cccggtgtcc
     1141 tttgcctctc ttcacgtgtc tccacgtcaa acctgatgtg tcagagctga tgtttgccga
     1201 ttgggtggaa tttagtccat atgagattgg catggcaaaa tatggtacct ttatggctcc
     1261 tgacctattt ggaagcaagt tttttatggg aacagttgta aaaaaatatg aagaaaaccc
     1321 cttgcatttc ttgatgggtg tctggggcag tgccttttct atactgttca acagagtttt
     1381 gggagtttct ggctcacaga ataaaggctc tacaatggaa gaggaattag aaaatattac
     1441 agcaaagcac atcgtgagta atgacagctc cgacagtgat gatgaggctc aaggacccaa
     1501 aggcaccgag aatgaagaag ctgaaaaaga gtaccaaagc gacaaccaag caagttgggt
     1561 ccatcggatg ctaatggcct tggtgagcga ctcggcttta ttcaataccc gagaaggacg
     1621 tgccggaaag gtgcataact tcatgctggg cttgaatctc aacacatcat atccactgtc
     1681 tcccctgaga gacttcagct ctcaggattc cttcgatgac gagctcgacg cagcggtagc
     1741 agatccagat gaatttgaac gaatatatga accactggat gtcaaaagta agaagattca
     1801 tgtggtagat agtgggctca catttaacct gccatatccc ttgattcttc gacctcagag
     1861 aggtgtggat cttatcatct cctttgactt ttctgcaagg ccgagtgaca ccagtccccc
     1921 tttcaaggaa cttctgcttg cagagaagtg ggcgaaaatg aacaagcttc cctttccaaa
     1981 gatcgatcct tatgtgtttg atcgggaagg attaaaggaa tgctatgttt ttaaacctaa
     2041 gaatcctgat gtggagaagg attgcccaac cattatccac tttgttctgg ccaacatcaa
     2101 cttcagaaag tacaaggccc caggtgttct aagggaaacc aaagaagaga aagaaattgc
     2161 tgactttgac atttttgatg accccgaatc gccattttca accttcaact ttcagtatcc
     2221 caatcaagca ttcaaaaggc ttcacgattt gatgtacttc aacacactga acaacattga
     2281 tgtgataaag gatgccattg ttgagagcat tgaatacaga agacagaacc catctcgttg
     2341 ctctgtttcc ctcagtaatg ttgaagcaag aaaattcttc aataaggagt ttctaagtaa
     2401 acccactgtg taatttctgt gctgggatga tcaagccatt tgaattccat gacaatttga
     2461 gttcagaaga cattagaggt catcttacta tgcagaagag actggctgct actcaaagtt
     2521 gtggagattt agccatgtgt taggtgaaaa tgatgttgat tatgtaatac ttagcaacag
     2581 tttctgacag tatgaatttt ttgacattag catagagcta tatactgtat tttaaacatt
     2641 cctcacattt tttacctgta ctttttatat aaatatgaca tgtcttttct tttgaaaata
     2701 tttaatagtt taactcagta aaggagactt cccattgtgt gtgaatgtta ttctgaacta
     2761 gatttgttca tgccatgtta caacactatt tttatttaaa tgtttatatt tacacatacg
     2821 aaataaatac tttgctgtac aaattaccac tagaaaaaaa aaaaaaaaa
//
