LOCUS       NM_001411864            4460 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus Pbx/knotted 1 homeobox (Pknox1), transcript variant
            16, mRNA.
ACCESSION   NM_001411864
VERSION     NM_001411864.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4460)
  AUTHORS   Cimmino,I., Prisco,F., Orso,S., Agognon,A.L., Liguoro,P., De
            Biase,D., Doti,N., Ruvo,M., Paciello,O., Beguinot,F., Formisano,P.
            and Oriente,F.
  TITLE     Interleukin 6 reduces vascular smooth muscle cell apoptosis via
            Prep1 and is associated with aging
  JOURNAL   FASEB J 35 (11), e21989 (2021)
   PUBMED   34679197
  REMARK    GeneRIF: Interleukin 6 reduces vascular smooth muscle cell
            apoptosis via Prep1 and is associated with aging.
REFERENCE   3  (bases 1 to 4460)
  AUTHORS   Cimmino,I., Margheri,F., Prisco,F., Perruolo,G., D'Esposito,V.,
            Laurenzana,A., Fibbi,G., Paciello,O., Doti,N., Ruvo,M., Miele,C.,
            Beguinot,F., Formisano,P. and Oriente,F.
  TITLE     Prep1 regulates angiogenesis through a PGC-1alpha-mediated
            mechanism
  JOURNAL   FASEB J 33 (12), 13893-13904 (2019)
   PUBMED   31618597
  REMARK    GeneRIF: Prep1, complexing with p160, decreases PGC-1alpha and
            stimulates angiogenesis.
REFERENCE   4  (bases 1 to 4460)
  AUTHORS   Volkel,S., Stielow,B., Finkernagel,F., Berger,D., Stiewe,T.,
            Nist,A. and Suske,G.
  TITLE     Transcription factor Sp2 potentiates binding of the TALE
            homeoproteins Pbx1:Prep1 and the histone-fold domain protein Nf-y
            to composite genomic sites
  JOURNAL   J Biol Chem 293 (50), 19250-19262 (2018)
   PUBMED   30337366
  REMARK    GeneRIF: a mechanism by which the DNA binding-independent activity
            of Sp2 potentiates genomic loading of Pbx1:Prep1 and Nf-y to
            composite motifs present in many promoters of highly expressed
            genes
REFERENCE   5  (bases 1 to 4460)
  AUTHORS   Sicouri,L., Pisati,F., Pece,S., Blasi,F. and Longobardi,E.
  TITLE     Prep1 (pKnox1) transcription factor contributes to pubertal mammary
            gland branching morphogenesis
  JOURNAL   Int J Dev Biol 62 (11-12), 827-836 (2018)
   PUBMED   30604852
  REMARK    GeneRIF: We conclude that Prep1 is required for normal adult
            mammary gland development, in particular at its branching
            morphogenesis step. By binding Snail2, Prep1 protects it from the
            proteasomal degradation.
REFERENCE   6  (bases 1 to 4460)
  AUTHORS   Jacobs,Y., Schnabel,C.A. and Cleary,M.L.
  TITLE     Trimeric association of Hox and TALE homeodomain proteins mediates
            Hoxb2 hindbrain enhancer activity
  JOURNAL   Mol Cell Biol 19 (7), 5134-5142 (1999)
   PUBMED   10373562
REFERENCE   7  (bases 1 to 4460)
  AUTHORS   Ferretti,E., Schulz,H., Talarico,D., Blasi,F. and Berthelsen,J.
  TITLE     The PBX-regulating protein PREP1 is present in different
            PBX-complexed forms in mouse
  JOURNAL   Mech Dev 83 (1-2), 53-64 (1999)
   PUBMED   10381567
REFERENCE   8  (bases 1 to 4460)
  AUTHORS   Ko,M.S., Threat,T.A., Wang,X., Horton,J.H., Cui,Y., Wang,X.,
            Pryor,E., Paris,J., Wells-Smith,J., Kitchen,J.R., Rowe,L.B.,
            Eppig,J., Satoh,T., Brant,L., Fujiwara,H., Yotsumoto,S. and
            Nakashima,H.
  TITLE     Genome-wide mapping of unselected transcripts from extraembryonic
            tissue of 7.5-day mouse embryos reveals enrichment in the t-complex
            and under-representation on the X chromosome
  JOURNAL   Hum Mol Genet 7 (12), 1967-1978 (1998)
   PUBMED   9811942
REFERENCE   9  (bases 1 to 4460)
  AUTHORS   Berthelsen,J., Viggiano,L., Schulz,H., Ferretti,E., Consalez,G.G.,
            Rocchi,M. and Blasi,F.
  TITLE     PKNOX1, a gene encoding PREP1, a new regulator of Pbx activity,
            maps on human chromosome 21q22.3 and murine chromosome 17B/C
  JOURNAL   Genomics 47 (2), 323-324 (1998)
   PUBMED   9479508
REFERENCE   10 (bases 1 to 4460)
  AUTHORS   Knoepfler,P.S., Calvo,K.R., Chen,H., Antonarakis,S.E. and
            Kamps,M.P.
  TITLE     Meis1 and pKnox1 bind DNA cooperatively with Pbx1 utilizing an
            interaction surface disrupted in oncoprotein E2a-Pbx1
  JOURNAL   Proc Natl Acad Sci U S A 94 (26), 14553-14558 (1997)
   PUBMED   9405651
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC166172.1 and AC166575.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK134000.1, SRR17253011.9186180.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-124               AC166172.1         1990-2113           c
            125-371             AC166575.1         44980-45226         c
            372-479             AC166575.1         43227-43334         c
            480-607             AC166575.1         38443-38570         c
            608-779             AC166575.1         36256-36427         c
            780-950             AC166575.1         34755-34925         c
            951-1050            AC166575.1         31706-31805         c
            1051-1148           AC166575.1         30137-30234         c
            1149-1277           AC166575.1         27387-27515         c
            1278-1354           AC166575.1         24168-24244         c
            1355-1527           AC166575.1         23671-23843         c
            1528-4460           AC166575.1         19345-22277         c
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 16.78 cM"
     gene            1..4460
                     /gene="Pknox1"
                     /gene_synonym="D17Wsu76e; PREP1"
                     /note="Pbx/knotted 1 homeobox"
                     /db_xref="GeneID:18771"
                     /db_xref="MGI:MGI:1201409"
     exon            1..124
                     /gene="Pknox1"
                     /gene_synonym="D17Wsu76e; PREP1"
                     /inference="alignment:Splign:2.1.0"
     exon            125..371
                     /gene="Pknox1"
                     /gene_synonym="D17Wsu76e; PREP1"
                     /inference="alignment:Splign:2.1.0"
     exon            372..479
                     /gene="Pknox1"
                     /gene_synonym="D17Wsu76e; PREP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             429..1739
                     /gene="Pknox1"
                     /gene_synonym="D17Wsu76e; PREP1"
                     /note="isoform 1 is encoded by transcript variant 16;
                     homeobox protein PKNOX1; PBX/knotted homeobox 1"
                     /codon_start=1
                     /product="homeobox protein PKNOX1 isoform 1"
                     /protein_id="NP_001398793.1"
                     /db_xref="GeneID:18771"
                     /db_xref="MGI:MGI:1201409"
                     /translation="MMATQTLSIDSYQDGQQMQVVTELKTEQDPNCSDPDAEGVSPPP
                     IESQTPMDADKQAIYRHPLFPLLALLFEKCEQSTQGSEGTTSASFDVDIENFVRKQEK
                     DGKPFFCEDPETDNLMVKAIQVLRIHLLELEKVNELCKDFCSRYIACLKTKMNSETLL
                     SGEPGSPYSPVQSQQIQSAITGTLSPQGIVVPASALQQGNVTMATVAGGTVYQPVTVV
                     TPQGQVVTQALSPGTIRIQNSQLQLQLNQDLSILHQEDGSSKNKRGVLPKHATNVMRS
                     WLFQHIGHPYPTEDEKKQIAAQTNLTLLQVNNWFINARRRILQPMLDSSCSETPKTKK
                     KPAQNRPVQRFWPDSLASGVAQATPSELAMSEGAVVTITTPVNMNVDSLQSLSSDGAT
                     LAVQQVMMAGQSEDESVDSTEDEGGALAPTHISGLVLENSDSLQ"
     exon            480..607
                     /gene="Pknox1"
                     /gene_synonym="D17Wsu76e; PREP1"
                     /inference="alignment:Splign:2.1.0"
     exon            608..779
                     /gene="Pknox1"
                     /gene_synonym="D17Wsu76e; PREP1"
                     /inference="alignment:Splign:2.1.0"
     exon            780..950
                     /gene="Pknox1"
                     /gene_synonym="D17Wsu76e; PREP1"
                     /inference="alignment:Splign:2.1.0"
     exon            951..1050
                     /gene="Pknox1"
                     /gene_synonym="D17Wsu76e; PREP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1051..1148
                     /gene="Pknox1"
                     /gene_synonym="D17Wsu76e; PREP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1149..1277
                     /gene="Pknox1"
                     /gene_synonym="D17Wsu76e; PREP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1278..1354
                     /gene="Pknox1"
                     /gene_synonym="D17Wsu76e; PREP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1355..1527
                     /gene="Pknox1"
                     /gene_synonym="D17Wsu76e; PREP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1528..4460
                     /gene="Pknox1"
                     /gene_synonym="D17Wsu76e; PREP1"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2002
                     /gene="Pknox1"
                     /gene_synonym="D17Wsu76e; PREP1"
     regulatory      4442..4447
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pknox1"
                     /gene_synonym="D17Wsu76e; PREP1"
                     /note="hexamer: ATTAAA"
     polyA_site      4460
                     /gene="Pknox1"
                     /gene_synonym="D17Wsu76e; PREP1"
                     /note="major polyA site"
ORIGIN      
        1 ctcctcccat tgagcgaggc tgtgtcgcgt ggcccggcgt cggcgtgacg gttggacgcg
       61 ggcgcggcac tgcgggtccc gattgctgca gccgcttgtc agtgtgatga agattggcac
      121 ccaggtgctg ggtgttagag tgggacgtgt cttgtggagg actcagctca tctccatgtg
      181 taccctctgt gccctgatgg tgaccaagtg attccagtcc cggctcctct gctcagtttc
      241 cggaaaggta tcttgagact cgtctgcagc ctgagatgtc ctgccttgtt tcccttccaa
      301 gccatggagc tgttcttaga tgtttgctcg tctcgggacc cgtgcacggc tctgttcttc
      361 caggtgctca gacaccgtgt gcttctcgct caagatgatc tgatgtctga agtggactct
      421 cactaaccat gatggcgaca cagacgctaa gtatagacag ctatcaagat ggacagcaaa
      481 tgcaggtggt cacggagtta aaaacagagc aagatcccaa ctgctctgac ccagatgcag
      541 aaggagtgag tcctcctccc atcgagtctc agaccccaat ggatgccgac aagcaggcca
      601 tttataggca tccactattt ccgttgctag ctttgttgtt tgagaagtgt gagcagtcca
      661 cacagggctc agaaggcacg acgtctgcca gcttcgatgt ggacattgag aactttgtca
      721 ggaagcaaga aaaggatggg aaacccttct tctgtgaaga tccggaaact gacaacctaa
      781 tggtgaaagc aatccaggtc ctgcgcattc atcttcttga actggagaag gttaatgagc
      841 tctgtaaaga tttctgtagt cggtacattg cttgtctgaa gacaaaaatg aacagcgaga
      901 ccttgttgag tggggagcct ggaagtccgt actcccctgt gcaatcccag cagattcaga
      961 gtgccatcac aggcacgctc agcccccagg gaatcgtggt gccagcatca gccctacaac
     1021 agggaaatgt aaccatggca acagtggcag gtggcacagt gtaccagcct gtcaccgtcg
     1081 tcactccgca aggccaagtg gtcacgcagg cattatctcc tgggacaatt aggatccaga
     1141 actcacagct gcagttgcag ttgaaccaag acctcagcat cttgcatcaa gaggatggct
     1201 cctccaagaa caagaggggt gtcctgccga agcacgccac caacgtgatg cggtcctggc
     1261 tctttcagca catagggcat ccctacccaa cagaggatga gaaaaagcag attgctgctc
     1321 agacgaatct gaccctgctt caagtcaaca actggttcat caacgcgaga agacgaattc
     1381 tccagccaat gttggattcc agctgctcag agactccaaa aacgaagaaa aaacctgctc
     1441 agaacaggcc agttcagagg ttttggccag attctcttgc atcaggagtg gcacaagcaa
     1501 cacccagcga gcttgccatg tcagaaggtg ccgttgtgac catcaccaca cctgtaaaca
     1561 tgaatgtgga cagccttcag tccctgtcct cagacggggc caccctggcc gtacagcagg
     1621 tcatgatggc agggcagagt gaggacgagt cggtggatag cacagaggat gagggcggcg
     1681 ccctggcgcc cacacacatc agcgggctgg tgctggagaa cagcgactcc cttcagtagg
     1741 aagcaccaac ggggtcagtg tggagctggg cgctggactc ttcactgttt gcacagcaag
     1801 catcttacag ttgtctttgt aacctgtttt atatgtagat atagaaggtg cacttttgta
     1861 tttcgcagca agcttcaaga cgtctttgcc ggtgcagcga cttccttcag atgtgcgtgt
     1921 atgggttttt aatgctagaa acgtggcccc tgccccttga gtccttgacg agattaagga
     1981 acagtgctgc cattttctaa aattctgcag ttgcaattga gtcgtgtggt ttacagcagg
     2041 tctgggggcg gctgctggtg ccgctcacag gtctgaaaga tgacctgcac gcaggctgca
     2101 gagcgagagg gctctggccc cggtgggctg tgggtgccca ccacacactt cagttctctc
     2161 aggcagcatt tgcctgtttc atttgctata tagaaaaaga aactcctatt tttaccttgc
     2221 tgggattatt ggataaaaag ctatttttat aaatcagtta ttaattggat tatgactata
     2281 ttgaggataa atttctagag aagcaacagc acatgcttgt ctttcgattc ggaatgttct
     2341 gaaaggcggc cacctgctca tggtctcctg ctctctgggg aggagagggc tcgcctcagc
     2401 tgctaaaagg aaacaactgt caagtgagct tttgacatct ccagtttctg ctcatgtgtg
     2461 atgcagactg ctacatttta cactcgctag agcatcttac acagtgtact cagtaggacg
     2521 tacggtaagt gagggctctg ccacgcctag tctgactgca gttccatggc tgcctggcta
     2581 tgttaactgg gtggtaattt tagatttttt ttttttagtt aaaacctgtt atatagagaa
     2641 tgtttattat aaactaatat aaagtgtgtt ctgccccact ggctcagagc tggtgtaaac
     2701 agcaaacacc taaactgtct ttcttactgg cagacaccta gaagttagaa ccatggctgt
     2761 aacgtggcgg tggggcccac tggtggctct cactgtcact cactctccag gctcctcccc
     2821 ctctctgtta ccactacaga ctcaggacag tttgaggacc tcatttctga gtgtcaaggc
     2881 tgagatttgg tttggtttgt ttgggtctac ttgggaagct ttttcttagg aaacaggcat
     2941 ctgtcttcac agtggtacct aggccacctg cagataggga acagctgtct aggggtccct
     3001 gccttgggcc ccacggttac cccacggtta cctctttagc taattcagga atagttcacc
     3061 atcaccccaa atgtcttgat tctcttcagc cattgagcac acctgaacag ccaagtagtc
     3121 agcaaaaaga atgggggctc cgtttgaacc acttaaggtg cgtccaagac agcaaaataa
     3181 aacccccaga acttgccagc aactcctttg aggagagaat actatttcaa gcagtctttc
     3241 ccttacctat ttacaaactg ttttaacagt ttaagtccgg catacaaaag cctgcacgat
     3301 cagctggctc tattaggttt agagcagaag tacatctctc ttgtgagccc tgcagagctc
     3361 tgccagtccc caaaatggaa gcacagcccc tgctgcacca gctggtggcg ctctagctgg
     3421 gattcgaaca ccatgctcta ccgtgtcctt tccagttgaa atcatctttc ctaagcacag
     3481 aactctagcc tgctaaatca aaggaatatc atgaagatcg taagagaacc ccaggcacgg
     3541 tgacacactc ctgtaatccc aacactcagg aacctgagac aggaggcaag cctgggctac
     3601 ttgaccctgt cttaaaaaaa aaaagctagt aaaagagctg gcacagggtc atatcctttc
     3661 acctccccgt gcacagagat gcttttaagt gctccccatg aggcgagcca tgctggttca
     3721 tagagtccct gcccagcaga tttggtgtct gccagttgca cctttgagac acctgcttgc
     3781 tgggcacaat ggcatattcc tgtaaacaag cacttaggag gctgaagcag gaggattgct
     3841 gcaaggtcga gttggcctct ggtgtgtagt gaaacccagt ttcaaaaaaa aaaaaaaaaa
     3901 atcgagcaaa actcctgctc gcctttcctt ggccagtgca gaaaaccttt gagctttgtt
     3961 taaatggtca gactcccaaa cattggagcc ttttgaatgt gttctgagac ctacagtcaa
     4021 ctcgtgtcat tcctgctgtt tggctgtcta gcagaaatct agactggctg acccactcct
     4081 ccggtgggct caggttttct gctttcttga ttccagattg tcaaatagaa gtgtatatgc
     4141 aatatgcact gtgacccttg cggctagggc tgacgggcca ctgtgtagcc tgctgggtgg
     4201 ccagtgcacc tgtgggggca gcgggagact tgctcctccc ccatggctgg cagggaaatg
     4261 cccatcccag aggcccctcc cgcaggttgt gactggaaca cagcaggctg ccttcttcag
     4321 ttaagttcgg tctttgaaac tgacaatctt taaaatgtga atactgtaac aatatgtttt
     4381 cttggattgt tgtctttaaa aggatttttg tgaagcaatt gatttatcaa agaaaaaaaa
     4441 aattaaaacc agaaacatgc
//
