LOCUS       NM_001411468            4216 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus Pbx/knotted 1 homeobox (Pknox1), transcript variant
            14, mRNA.
ACCESSION   NM_001411468
VERSION     NM_001411468.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Cimmino,I., Prisco,F., Orso,S., Agognon,A.L., Liguoro,P., De
            Biase,D., Doti,N., Ruvo,M., Paciello,O., Beguinot,F., Formisano,P.
            and Oriente,F.
  TITLE     Interleukin 6 reduces vascular smooth muscle cell apoptosis via
            Prep1 and is associated with aging
  JOURNAL   FASEB J 35 (11), e21989 (2021)
   PUBMED   34679197
  REMARK    GeneRIF: Interleukin 6 reduces vascular smooth muscle cell
            apoptosis via Prep1 and is associated with aging.
REFERENCE   3  (bases 1 to 4216)
  AUTHORS   Cimmino,I., Margheri,F., Prisco,F., Perruolo,G., D'Esposito,V.,
            Laurenzana,A., Fibbi,G., Paciello,O., Doti,N., Ruvo,M., Miele,C.,
            Beguinot,F., Formisano,P. and Oriente,F.
  TITLE     Prep1 regulates angiogenesis through a PGC-1alpha-mediated
            mechanism
  JOURNAL   FASEB J 33 (12), 13893-13904 (2019)
   PUBMED   31618597
  REMARK    GeneRIF: Prep1, complexing with p160, decreases PGC-1alpha and
            stimulates angiogenesis.
REFERENCE   4  (bases 1 to 4216)
  AUTHORS   Volkel,S., Stielow,B., Finkernagel,F., Berger,D., Stiewe,T.,
            Nist,A. and Suske,G.
  TITLE     Transcription factor Sp2 potentiates binding of the TALE
            homeoproteins Pbx1:Prep1 and the histone-fold domain protein Nf-y
            to composite genomic sites
  JOURNAL   J Biol Chem 293 (50), 19250-19262 (2018)
   PUBMED   30337366
  REMARK    GeneRIF: a mechanism by which the DNA binding-independent activity
            of Sp2 potentiates genomic loading of Pbx1:Prep1 and Nf-y to
            composite motifs present in many promoters of highly expressed
            genes
REFERENCE   5  (bases 1 to 4216)
  AUTHORS   Sicouri,L., Pisati,F., Pece,S., Blasi,F. and Longobardi,E.
  TITLE     Prep1 (pKnox1) transcription factor contributes to pubertal mammary
            gland branching morphogenesis
  JOURNAL   Int J Dev Biol 62 (11-12), 827-836 (2018)
   PUBMED   30604852
  REMARK    GeneRIF: We conclude that Prep1 is required for normal adult
            mammary gland development, in particular at its branching
            morphogenesis step. By binding Snail2, Prep1 protects it from the
            proteasomal degradation.
REFERENCE   6  (bases 1 to 4216)
  AUTHORS   Jacobs,Y., Schnabel,C.A. and Cleary,M.L.
  TITLE     Trimeric association of Hox and TALE homeodomain proteins mediates
            Hoxb2 hindbrain enhancer activity
  JOURNAL   Mol Cell Biol 19 (7), 5134-5142 (1999)
   PUBMED   10373562
REFERENCE   7  (bases 1 to 4216)
  AUTHORS   Ferretti,E., Schulz,H., Talarico,D., Blasi,F. and Berthelsen,J.
  TITLE     The PBX-regulating protein PREP1 is present in different
            PBX-complexed forms in mouse
  JOURNAL   Mech Dev 83 (1-2), 53-64 (1999)
   PUBMED   10381567
REFERENCE   8  (bases 1 to 4216)
  AUTHORS   Ko,M.S., Threat,T.A., Wang,X., Horton,J.H., Cui,Y., Wang,X.,
            Pryor,E., Paris,J., Wells-Smith,J., Kitchen,J.R., Rowe,L.B.,
            Eppig,J., Satoh,T., Brant,L., Fujiwara,H., Yotsumoto,S. and
            Nakashima,H.
  TITLE     Genome-wide mapping of unselected transcripts from extraembryonic
            tissue of 7.5-day mouse embryos reveals enrichment in the t-complex
            and under-representation on the X chromosome
  JOURNAL   Hum Mol Genet 7 (12), 1967-1978 (1998)
   PUBMED   9811942
REFERENCE   9  (bases 1 to 4216)
  AUTHORS   Berthelsen,J., Viggiano,L., Schulz,H., Ferretti,E., Consalez,G.G.,
            Rocchi,M. and Blasi,F.
  TITLE     PKNOX1, a gene encoding PREP1, a new regulator of Pbx activity,
            maps on human chromosome 21q22.3 and murine chromosome 17B/C
  JOURNAL   Genomics 47 (2), 323-324 (1998)
   PUBMED   9479508
REFERENCE   10 (bases 1 to 4216)
  AUTHORS   Knoepfler,P.S., Calvo,K.R., Chen,H., Antonarakis,S.E. and
            Kamps,M.P.
  TITLE     Meis1 and pKnox1 bind DNA cooperatively with Pbx1 utilizing an
            interaction surface disrupted in oncoprotein E2a-Pbx1
  JOURNAL   Proc Natl Acad Sci U S A 94 (26), 14553-14558 (1997)
   PUBMED   9405651
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC166172.1 and AC166575.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: ERR3363658.3535938.1,
                                           SRR13861859.3896851.1 [ECO:0000332]
            RNAseq introns              :: partial sample support SAMN00849374,
                                           SAMN00849375 [ECO:0000350]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-124               AC166172.1         1990-2113           c
            125-232             AC166575.1         43227-43334         c
            233-363             AC166575.1         38440-38570         c
            364-535             AC166575.1         36256-36427         c
            536-706             AC166575.1         34755-34925         c
            707-806             AC166575.1         31706-31805         c
            807-904             AC166575.1         30137-30234         c
            905-1033            AC166575.1         27387-27515         c
            1034-1110           AC166575.1         24168-24244         c
            1111-1283           AC166575.1         23671-23843         c
            1284-4216           AC166575.1         19345-22277         c
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 16.78 cM"
     gene            1..4216
                     /gene="Pknox1"
                     /gene_synonym="D17Wsu76e; PREP1"
                     /note="Pbx/knotted 1 homeobox"
                     /db_xref="GeneID:18771"
                     /db_xref="MGI:MGI:1201409"
     exon            1..124
                     /gene="Pknox1"
                     /gene_synonym="D17Wsu76e; PREP1"
                     /inference="alignment:Splign:2.1.0"
     exon            125..232
                     /gene="Pknox1"
                     /gene_synonym="D17Wsu76e; PREP1"
                     /inference="alignment:Splign:2.1.0"
     exon            233..363
                     /gene="Pknox1"
                     /gene_synonym="D17Wsu76e; PREP1"
                     /inference="alignment:Splign:2.1.0"
     exon            364..535
                     /gene="Pknox1"
                     /gene_synonym="D17Wsu76e; PREP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             536..1495
                     /gene="Pknox1"
                     /gene_synonym="D17Wsu76e; PREP1"
                     /note="isoform 6 is encoded by transcript variant 14;
                     homeobox protein PKNOX1; PBX/knotted homeobox 1"
                     /codon_start=1
                     /product="homeobox protein PKNOX1 isoform 6"
                     /protein_id="NP_001398397.1"
                     /db_xref="GeneID:18771"
                     /db_xref="MGI:MGI:1201409"
                     /translation="MVKAIQVLRIHLLELEKVNELCKDFCSRYIACLKTKMNSETLLS
                     GEPGSPYSPVQSQQIQSAITGTLSPQGIVVPASALQQGNVTMATVAGGTVYQPVTVVT
                     PQGQVVTQALSPGTIRIQNSQLQLQLNQDLSILHQEDGSSKNKRGVLPKHATNVMRSW
                     LFQHIGHPYPTEDEKKQIAAQTNLTLLQVNNWFINARRRILQPMLDSSCSETPKTKKK
                     PAQNRPVQRFWPDSLASGVAQATPSELAMSEGAVVTITTPVNMNVDSLQSLSSDGATL
                     AVQQVMMAGQSEDESVDSTEDEGGALAPTHISGLVLENSDSLQ"
     exon            536..706
                     /gene="Pknox1"
                     /gene_synonym="D17Wsu76e; PREP1"
                     /inference="alignment:Splign:2.1.0"
     exon            707..806
                     /gene="Pknox1"
                     /gene_synonym="D17Wsu76e; PREP1"
                     /inference="alignment:Splign:2.1.0"
     exon            807..904
                     /gene="Pknox1"
                     /gene_synonym="D17Wsu76e; PREP1"
                     /inference="alignment:Splign:2.1.0"
     exon            905..1033
                     /gene="Pknox1"
                     /gene_synonym="D17Wsu76e; PREP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1034..1110
                     /gene="Pknox1"
                     /gene_synonym="D17Wsu76e; PREP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1111..1283
                     /gene="Pknox1"
                     /gene_synonym="D17Wsu76e; PREP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1284..4216
                     /gene="Pknox1"
                     /gene_synonym="D17Wsu76e; PREP1"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1758
                     /gene="Pknox1"
                     /gene_synonym="D17Wsu76e; PREP1"
     regulatory      4198..4203
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pknox1"
                     /gene_synonym="D17Wsu76e; PREP1"
                     /note="hexamer: ATTAAA"
     polyA_site      4216
                     /gene="Pknox1"
                     /gene_synonym="D17Wsu76e; PREP1"
                     /note="major polyA site"
ORIGIN      
        1 ctcctcccat tgagcgaggc tgtgtcgcgt ggcccggcgt cggcgtgacg gttggacgcg
       61 ggcgcggcac tgcgggtccc gattgctgca gccgcttgtc agtgtgatga agattggcac
      121 ccagacaccg tgtgcttctc gctcaagatg atctgatgtc tgaagtggac tctcactaac
      181 catgatggcg acacagacgc taagtataga cagctatcaa gatggacagc aaatgcaggt
      241 ggtcacggag ttaaaaacag agcaagatcc caactgctct gacccagatg cagaaggagt
      301 gagtcctcct cccatcgagt ctcagacccc aatggatgcc gacaagcagg ccatttatag
      361 gtagcatcca ctatttccgt tgctagcttt gttgtttgag aagtgtgagc agtccacaca
      421 gggctcagaa ggcacgacgt ctgccagctt cgatgtggac attgagaact ttgtcaggaa
      481 gcaagaaaag gatgggaaac ccttcttctg tgaagatccg gaaactgaca acctaatggt
      541 gaaagcaatc caggtcctgc gcattcatct tcttgaactg gagaaggtta atgagctctg
      601 taaagatttc tgtagtcggt acattgcttg tctgaagaca aaaatgaaca gcgagacctt
      661 gttgagtggg gagcctggaa gtccgtactc ccctgtgcaa tcccagcaga ttcagagtgc
      721 catcacaggc acgctcagcc cccagggaat cgtggtgcca gcatcagccc tacaacaggg
      781 aaatgtaacc atggcaacag tggcaggtgg cacagtgtac cagcctgtca ccgtcgtcac
      841 tccgcaaggc caagtggtca cgcaggcatt atctcctggg acaattagga tccagaactc
      901 acagctgcag ttgcagttga accaagacct cagcatcttg catcaagagg atggctcctc
      961 caagaacaag aggggtgtcc tgccgaagca cgccaccaac gtgatgcggt cctggctctt
     1021 tcagcacata gggcatccct acccaacaga ggatgagaaa aagcagattg ctgctcagac
     1081 gaatctgacc ctgcttcaag tcaacaactg gttcatcaac gcgagaagac gaattctcca
     1141 gccaatgttg gattccagct gctcagagac tccaaaaacg aagaaaaaac ctgctcagaa
     1201 caggccagtt cagaggtttt ggccagattc tcttgcatca ggagtggcac aagcaacacc
     1261 cagcgagctt gccatgtcag aaggtgccgt tgtgaccatc accacacctg taaacatgaa
     1321 tgtggacagc cttcagtccc tgtcctcaga cggggccacc ctggccgtac agcaggtcat
     1381 gatggcaggg cagagtgagg acgagtcggt ggatagcaca gaggatgagg gcggcgccct
     1441 ggcgcccaca cacatcagcg ggctggtgct ggagaacagc gactcccttc agtaggaagc
     1501 accaacgggg tcagtgtgga gctgggcgct ggactcttca ctgtttgcac agcaagcatc
     1561 ttacagttgt ctttgtaacc tgttttatat gtagatatag aaggtgcact tttgtatttc
     1621 gcagcaagct tcaagacgtc tttgccggtg cagcgacttc cttcagatgt gcgtgtatgg
     1681 gtttttaatg ctagaaacgt ggcccctgcc ccttgagtcc ttgacgagat taaggaacag
     1741 tgctgccatt ttctaaaatt ctgcagttgc aattgagtcg tgtggtttac agcaggtctg
     1801 ggggcggctg ctggtgccgc tcacaggtct gaaagatgac ctgcacgcag gctgcagagc
     1861 gagagggctc tggccccggt gggctgtggg tgcccaccac acacttcagt tctctcaggc
     1921 agcatttgcc tgtttcattt gctatataga aaaagaaact cctattttta ccttgctggg
     1981 attattggat aaaaagctat ttttataaat cagttattaa ttggattatg actatattga
     2041 ggataaattt ctagagaagc aacagcacat gcttgtcttt cgattcggaa tgttctgaaa
     2101 ggcggccacc tgctcatggt ctcctgctct ctggggagga gagggctcgc ctcagctgct
     2161 aaaaggaaac aactgtcaag tgagcttttg acatctccag tttctgctca tgtgtgatgc
     2221 agactgctac attttacact cgctagagca tcttacacag tgtactcagt aggacgtacg
     2281 gtaagtgagg gctctgccac gcctagtctg actgcagttc catggctgcc tggctatgtt
     2341 aactgggtgg taattttaga tttttttttt ttagttaaaa cctgttatat agagaatgtt
     2401 tattataaac taatataaag tgtgttctgc cccactggct cagagctggt gtaaacagca
     2461 aacacctaaa ctgtctttct tactggcaga cacctagaag ttagaaccat ggctgtaacg
     2521 tggcggtggg gcccactggt ggctctcact gtcactcact ctccaggctc ctccccctct
     2581 ctgttaccac tacagactca ggacagtttg aggacctcat ttctgagtgt caaggctgag
     2641 atttggtttg gtttgtttgg gtctacttgg gaagcttttt cttaggaaac aggcatctgt
     2701 cttcacagtg gtacctaggc cacctgcaga tagggaacag ctgtctaggg gtccctgcct
     2761 tgggccccac ggttacccca cggttacctc tttagctaat tcaggaatag ttcaccatca
     2821 ccccaaatgt cttgattctc ttcagccatt gagcacacct gaacagccaa gtagtcagca
     2881 aaaagaatgg gggctccgtt tgaaccactt aaggtgcgtc caagacagca aaataaaacc
     2941 cccagaactt gccagcaact cctttgagga gagaatacta tttcaagcag tctttccctt
     3001 acctatttac aaactgtttt aacagtttaa gtccggcata caaaagcctg cacgatcagc
     3061 tggctctatt aggtttagag cagaagtaca tctctcttgt gagccctgca gagctctgcc
     3121 agtccccaaa atggaagcac agcccctgct gcaccagctg gtggcgctct agctgggatt
     3181 cgaacaccat gctctaccgt gtcctttcca gttgaaatca tctttcctaa gcacagaact
     3241 ctagcctgct aaatcaaagg aatatcatga agatcgtaag agaaccccag gcacggtgac
     3301 acactcctgt aatcccaaca ctcaggaacc tgagacagga ggcaagcctg ggctacttga
     3361 ccctgtctta aaaaaaaaaa gctagtaaaa gagctggcac agggtcatat cctttcacct
     3421 ccccgtgcac agagatgctt ttaagtgctc cccatgaggc gagccatgct ggttcataga
     3481 gtccctgccc agcagatttg gtgtctgcca gttgcacctt tgagacacct gcttgctggg
     3541 cacaatggca tattcctgta aacaagcact taggaggctg aagcaggagg attgctgcaa
     3601 ggtcgagttg gcctctggtg tgtagtgaaa cccagtttca aaaaaaaaaa aaaaaaatcg
     3661 agcaaaactc ctgctcgcct ttccttggcc agtgcagaaa acctttgagc tttgtttaaa
     3721 tggtcagact cccaaacatt ggagcctttt gaatgtgttc tgagacctac agtcaactcg
     3781 tgtcattcct gctgtttggc tgtctagcag aaatctagac tggctgaccc actcctccgg
     3841 tgggctcagg ttttctgctt tcttgattcc agattgtcaa atagaagtgt atatgcaata
     3901 tgcactgtga cccttgcggc tagggctgac gggccactgt gtagcctgct gggtggccag
     3961 tgcacctgtg ggggcagcgg gagacttgct cctcccccat ggctggcagg gaaatgccca
     4021 tcccagaggc ccctcccgca ggttgtgact ggaacacagc aggctgcctt cttcagttaa
     4081 gttcggtctt tgaaactgac aatctttaaa atgtgaatac tgtaacaata tgttttcttg
     4141 gattgttgtc tttaaaagga tttttgtgaa gcaattgatt tatcaaagaa aaaaaaaatt
     4201 aaaaccagaa acatgc
//
