LOCUS       NM_178654               6207 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus protein kinase N2 (Pkn2), mRNA.
ACCESSION   NM_178654
VERSION     NM_178654.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6207)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 6207)
  AUTHORS   Marshall,J.J.T., Cull,J.J., Alharbi,H.O., Zaw Thin,M.,
            Cooper,S.T.E., Barrington,C., Vanyai,H., Snoeks,T., Siow,B.,
            Suaarez-Bonnet,A., Herbert,E., Stuckey,D.J., Cameron,A.J.M.,
            Prin,F., Cook,A.C., Priestnall,S.L., Chotani,S., Rackham,O.J.L.,
            Meijles,D.N., Mohun,T., Clerk,A. and Parker,P.J.
  TITLE     PKN2 deficiency leads both to prenatal 'congenital' cardiomyopathy
            and defective angiotensin II stress responses
  JOURNAL   Biochem J 479 (13), 1467-1486 (2022)
   PUBMED   35730579
  REMARK    GeneRIF: PKN2 deficiency leads both to prenatal 'congenital'
            cardiomyopathy and defective angiotensin II stress responses.
REFERENCE   3  (bases 1 to 6207)
  AUTHORS   Murray,E.R., Menezes,S., Henry,J.C., Williams,J.L.,
            Alba-Castellon,L., Baskaran,P., Quetier,I., Desai,A.,
            Marshall,J.J.T., Rosewell,I., Tatari,M., Rajeeve,V., Khan,F.,
            Wang,J., Kotantaki,P., Tyler,E.J., Singh,N., Reader,C.S.,
            Carter,E.P., Hodivala-Dilke,K., Grose,R.P., Kocher,H.M., Gavara,N.,
            Pearce,O., Cutillas,P., Marshall,J.F. and Cameron,A.J.M.
  TITLE     Disruption of pancreatic stellate cell myofibroblast phenotype
            promotes pancreatic tumor invasion
  JOURNAL   Cell Rep 38 (4), 110227 (2022)
   PUBMED   35081338
REFERENCE   4  (bases 1 to 6207)
  AUTHORS   Jin,Y.J., Chennupati,R., Li,R., Liang,G., Wang,S., Iring,A.,
            Graumann,J., Wettschureck,N. and Offermanns,S.
  TITLE     Protein kinase N2 mediates flow-induced endothelial NOS activation
            and vascular tone regulation
  JOURNAL   J Clin Invest 131 (21) (2021)
   PUBMED   34499618
  REMARK    GeneRIF: Protein kinase N2 mediates flow-induced endothelial NOS
            activation and vascular tone regulation.
REFERENCE   5  (bases 1 to 6207)
  AUTHORS   Patel,H., Li,J., Herrero,A., Kroboth,J., Byron,A., Kriegsheim,A.V.,
            Brunton,V., Carragher,N., Hurd,T. and Frame,M.
  TITLE     Novel roles of PRK1 and PRK2 in cilia and cancer biology
  JOURNAL   Sci Rep 10 (1), 3902 (2020)
   PUBMED   32127582
  REMARK    GeneRIF: Novel roles of PRK1 and PRK2 in cilia and cancer biology.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6207)
  AUTHORS   McPhee,J.B., Mena,P. and Bliska,J.B.
  TITLE     Delineation of regions of the Yersinia YopM protein required for
            interaction with the RSK1 and PRK2 host kinases and their
            requirement for interleukin-10 production and virulence
  JOURNAL   Infect Immun 78 (8), 3529-3539 (2010)
   PUBMED   20515922
  REMARK    GeneRIF: Yersinia pseudotuberculosis mutants expressing YopM
            proteins unable to interact with either RSK1 or PRK2 were defective
            for virulence in this assay, indicating that both interaction
            domains are important for YopM to promote pathogenesis.
REFERENCE   7  (bases 1 to 6207)
  AUTHORS   Torbett,N.E., Casamassima,A. and Parker,P.J.
  TITLE     Hyperosmotic-induced protein kinase N 1 activation in a vesicular
            compartment is dependent upon Rac1 and 3-phosphoinositide-dependent
            kinase 1
  JOURNAL   J Biol Chem 278 (34), 32344-32351 (2003)
   PUBMED   12783890
REFERENCE   8  (bases 1 to 6207)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   9  (bases 1 to 6207)
  AUTHORS   Hasenkrug,K.J. and Dittmer,U.
  TITLE     The role of CD4 and CD8 T cells in recovery and protection from
            retroviral infection: lessons from the Friend virus model
  JOURNAL   Virology 272 (2), 244-249 (2000)
   PUBMED   10873767
  REMARK    Review article
REFERENCE   10 (bases 1 to 6207)
  AUTHORS   Quilliam,L.A., Lambert,Q.T., Mickelson-Young,L.A., Westwick,J.K.,
            Sparks,A.B., Kay,B.K., Jenkins,N.A., Gilbert,D.J., Copeland,N.G.
            and Der,C.J.
  TITLE     Isolation of a NCK-associated kinase, PRK2, an SH3-binding protein
            and potential effector of Rho protein signaling
  JOURNAL   J Biol Chem 271 (46), 28772-28776 (1996)
   PUBMED   8910519
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY729073.1, AK133298.1, AK049713.1 and AK140746.1.
            
            On Sep 30, 2009 this sequence version replaced NM_178654.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK133298.1, AK171092.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-11                BY729073.1         1-11
            12-2895             AK133298.1         1-2884
            2896-3650           AK049713.1         2883-3637
            3651-6207           AK140746.1         180-2736
FEATURES             Location/Qualifiers
     source          1..6207
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 66.69 cM"
     gene            1..6207
                     /gene="Pkn2"
                     /gene_synonym="6030436C20Rik; PRK2; Prkcl2; Stk7"
                     /note="protein kinase N2"
                     /db_xref="GeneID:109333"
                     /db_xref="MGI:MGI:109211"
     exon            1..394
                     /gene="Pkn2"
                     /gene_synonym="6030436C20Rik; PRK2; Prkcl2; Stk7"
                     /inference="alignment:Splign:2.1.0"
     CDS             347..3298
                     /gene="Pkn2"
                     /gene_synonym="6030436C20Rik; PRK2; Prkcl2; Stk7"
                     /EC_number="2.7.11.13"
                     /note="serine/threonine kinase 7; protein kinase C-like 2;
                     protein-kinase C-related kinase 2; PKN gamma"
                     /codon_start=1
                     /product="serine/threonine-protein kinase N2"
                     /protein_id="NP_848769.2"
                     /db_xref="CCDS:CCDS51084.1"
                     /db_xref="GeneID:109333"
                     /db_xref="MGI:MGI:109211"
                     /translation="MASNPDRGEILLTELQGDSRTLPFSENVSAVQKLDFSDTMVQQK
                     LDDIKDRIKREIRKELKIKEGAENLRKVTTDKKNLAYVDNILKKSNKKLEELHHKLQE
                     LNAHIVVSDPEDSTDCPRTPDTPNSDSRSSTSNNRLMALQKQLDIELKVKQGAENMIQ
                     MYSNGSSKDRKLHGTAQQLLQDSKTKIEVIRMQILQAVQTNELAFDNAKPVISPLELR
                     MEELRHHFKIEFAVAEGAKNVMKLLGSGKVTDRKALSEAQARFNESSQKLDLLKYSLE
                     QRLNELPRNHPKSSVVIEELSLVASPTLSPRQSMLSTQNQYSTLSKPAALTGTLEVRL
                     MGCQDILENVPGRSKATSVALPGWSPSDNRSSFMSRTSKSKSGSSRNLLKTDDLSNDV
                     CAVLKLDNTVVGQTSWKPISNQSWDQKFTLELDRSRELEISVYWRDWRSLCAVKFLRL
                     EDFLDNQRHGMCLYLEPQGTLFAEVTFFNPVIERRPKLQRQKKIFSKQQGKTFLRAPQ
                     MNINIATWGRLVRRAIPTVNHSGTFSPQTPVPATVPVVDARIPDLAPPASDSTVTKLD
                     FDLEPEPPPAPPRASSLGETDESSELRVLDIPGQGSETVFNIENDRNNLRPKSKSEYE
                     LSIPDSGRSCWGVGELDDKRAQQRFQFSLQDFRCCAVLGRGHFGKVLLAEYKHTNEMF
                     AIKALKKGDIVARDEVDSLMCEKRIFETVNSVRHPFLVNLFACFQTKEHVCFVMEYAA
                     GGDLMMHIHTDVFSEPRAVFYAACVVLGLQYLHEHKIVYRDLKLDNLLLDTEGFVKIA
                     DFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTETSYTRAVDWWGLGVLIYEMLVGESPF
                     PGDDEEEVFDSIVNDEVRYPRFLSTEAISIMRRLLRRNPERRLGAGEKDAEDVKKHPF
                     FRLTDWSALMDKKVKPPFVPTIRGREDVSNFDDEFTSEAPILTPPREPRILLEEEQEM
                     FHDFDYVADWC"
     exon            395..695
                     /gene="Pkn2"
                     /gene_synonym="6030436C20Rik; PRK2; Prkcl2; Stk7"
                     /inference="alignment:Splign:2.1.0"
     exon            696..850
                     /gene="Pkn2"
                     /gene_synonym="6030436C20Rik; PRK2; Prkcl2; Stk7"
                     /inference="alignment:Splign:2.1.0"
     exon            851..968
                     /gene="Pkn2"
                     /gene_synonym="6030436C20Rik; PRK2; Prkcl2; Stk7"
                     /inference="alignment:Splign:2.1.0"
     exon            969..1114
                     /gene="Pkn2"
                     /gene_synonym="6030436C20Rik; PRK2; Prkcl2; Stk7"
                     /inference="alignment:Splign:2.1.0"
     exon            1115..1328
                     /gene="Pkn2"
                     /gene_synonym="6030436C20Rik; PRK2; Prkcl2; Stk7"
                     /inference="alignment:Splign:2.1.0"
     exon            1329..1514
                     /gene="Pkn2"
                     /gene_synonym="6030436C20Rik; PRK2; Prkcl2; Stk7"
                     /inference="alignment:Splign:2.1.0"
     exon            1515..1624
                     /gene="Pkn2"
                     /gene_synonym="6030436C20Rik; PRK2; Prkcl2; Stk7"
                     /inference="alignment:Splign:2.1.0"
     exon            1625..1768
                     /gene="Pkn2"
                     /gene_synonym="6030436C20Rik; PRK2; Prkcl2; Stk7"
                     /inference="alignment:Splign:2.1.0"
     exon            1769..1844
                     /gene="Pkn2"
                     /gene_synonym="6030436C20Rik; PRK2; Prkcl2; Stk7"
                     /inference="alignment:Splign:2.1.0"
     exon            1845..2019
                     /gene="Pkn2"
                     /gene_synonym="6030436C20Rik; PRK2; Prkcl2; Stk7"
                     /inference="alignment:Splign:2.1.0"
     exon            2020..2146
                     /gene="Pkn2"
                     /gene_synonym="6030436C20Rik; PRK2; Prkcl2; Stk7"
                     /inference="alignment:Splign:2.1.0"
     exon            2147..2277
                     /gene="Pkn2"
                     /gene_synonym="6030436C20Rik; PRK2; Prkcl2; Stk7"
                     /inference="alignment:Splign:2.1.0"
     exon            2278..2353
                     /gene="Pkn2"
                     /gene_synonym="6030436C20Rik; PRK2; Prkcl2; Stk7"
                     /inference="alignment:Splign:2.1.0"
     exon            2354..2445
                     /gene="Pkn2"
                     /gene_synonym="6030436C20Rik; PRK2; Prkcl2; Stk7"
                     /inference="alignment:Splign:2.1.0"
     exon            2446..2622
                     /gene="Pkn2"
                     /gene_synonym="6030436C20Rik; PRK2; Prkcl2; Stk7"
                     /inference="alignment:Splign:2.1.0"
     exon            2623..2685
                     /gene="Pkn2"
                     /gene_synonym="6030436C20Rik; PRK2; Prkcl2; Stk7"
                     /inference="alignment:Splign:2.1.0"
     exon            2686..2762
                     /gene="Pkn2"
                     /gene_synonym="6030436C20Rik; PRK2; Prkcl2; Stk7"
                     /inference="alignment:Splign:2.1.0"
     exon            2763..2905
                     /gene="Pkn2"
                     /gene_synonym="6030436C20Rik; PRK2; Prkcl2; Stk7"
                     /inference="alignment:Splign:2.1.0"
     exon            2906..3013
                     /gene="Pkn2"
                     /gene_synonym="6030436C20Rik; PRK2; Prkcl2; Stk7"
                     /inference="alignment:Splign:2.1.0"
     exon            3014..3094
                     /gene="Pkn2"
                     /gene_synonym="6030436C20Rik; PRK2; Prkcl2; Stk7"
                     /inference="alignment:Splign:2.1.0"
     exon            3095..6207
                     /gene="Pkn2"
                     /gene_synonym="6030436C20Rik; PRK2; Prkcl2; Stk7"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5575..5580
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pkn2"
                     /gene_synonym="6030436C20Rik; PRK2; Prkcl2; Stk7"
                     /note="hexamer: AATAGA"
     polyA_site      5593
                     /gene="Pkn2"
                     /gene_synonym="6030436C20Rik; PRK2; Prkcl2; Stk7"
                     /note="major polyA site"
ORIGIN      
        1 ttttgcgacg cggtgacagc caaatggtgc gacaggagga gctgccgccg cggccgctgc
       61 gccccgcacc gagagctccg agtcggctcc cggcgtcgcc ctgagggagc ggctgaggga
      121 acagagtccg gcgggcggcg gcgggcggcg aggggagccg cgccgcgagg gccctcgctc
      181 cccgccgctc tccatgagcc gcgcctgcaa gccgggccgc gcctccatga ctccctagct
      241 gccgctttct ctccggtcct cgccccccgc ccggctctcc cgtcccgcct tctccctcgg
      301 ccgcgccgct gggcgccttc aggtgcggag gcccgagcgg agcgccatgg cgtccaaccc
      361 cgaccggggg gagatcctcc tcaccgagct gcagggagat tcccggactc ttccattttc
      421 tgagaatgtg agtgctgttc agaaattaga cttttcagat acaatggtgc aacagaaatt
      481 ggacgacatc aaggatcgaa taaagagaga aataaggaaa gaactgaaaa tcaaagaagg
      541 agctgaaaac ctgaggaaag ttacaacaga taagaaaaat ttggcttatg tagacaacat
      601 tttgaaaaaa tcaaataaaa aattagaaga attacatcac aagctacaag aattaaatgc
      661 acacatcgtt gtatcggatc cagaggactc cacagattgt ccgaggactc cagatactcc
      721 aaatagtgac tctcgtagtt ctactagcaa caatagactg atggccttac agaaacaact
      781 ggatatagaa cttaaagtaa agcagggtgc agagaacatg atacagatgt actcaaatgg
      841 ctcttcaaag gatcggaaac tccatggtac agcgcagcaa ctgctacagg atagcaagac
      901 aaaaatagaa gtcatccgga tgcagattct tcaggccgtc cagaccaatg agctggcttt
      961 tgataatgca aaacctgtga taagccctct tgagcttcgg atggaagaat taagacatca
     1021 ttttaagata gaatttgcag tagccgaagg tgcaaagaat gtcatgaagt tacttggctc
     1081 aggaaaagta acagacagaa aagcgctttc agaagcacaa gcgagattta atgaatcaag
     1141 tcagaagttg gaccttttaa agtattcact agagcaaagg ttaaatgagc tccccaggaa
     1201 tcaccccaaa agcagtgtcg tcattgagga gctttcactg gttgcgtcac caaccctgag
     1261 tccacggcag agcatgctgt ccacgcagaa ccagtatagc acactgtcca agccagcggc
     1321 gctaaccggt actttggaag ttcgtcttat ggggtgccaa gatatcctag agaatgtacc
     1381 tggacggtca aaagcaacgt cggtagcact acctgggtgg agtccaagtg acaacagatc
     1441 gtcttttatg agcagaacca gcaagagtaa aagtgggagt agtcgcaacc tcctgaaaac
     1501 ggatgacttg tccaatgatg tttgtgctgt tttgaagcta gataatactg tggttggtca
     1561 aactagctgg aaacccattt caaatcagtc gtgggaccag aagtttacac tggaactaga
     1621 caggtcacgt gaactggaaa tttcagttta ttggcgtgat tggcgatctc tgtgtgctgt
     1681 aaaatttctg aggttagaag attttttaga caaccagcgg catggcatgt gtctctattt
     1741 ggaaccacag ggtactttgt ttgcagaggt tacttttttt aatccagtta ttgaaagacg
     1801 accaaaactc caaagacaaa agaaaatttt ttcaaaacaa caaggcaaaa catttctcag
     1861 agctcctcag atgaatatta atattgccac ttggggaaga ctagtgagac gagctattcc
     1921 cacagtaaat cattctggca cattcagccc tcagactcct gtgcctgcaa cagtgccagt
     1981 cgttgacgca cgcattcctg atctagcacc tccggccagt gactctacag taaccaaatt
     2041 ggactttgat cttgaacctg aacctcctcc agccccacct cgtgcttctt cccttggaga
     2101 gacagatgaa tcttctgaat tgagagtttt ggatatacct ggacagggtt cagaaactgt
     2161 tttcaatatt gagaatgaca gaaataacct gcgtccaaaa tccaaatctg aatatgagct
     2221 gagcattcct gactcaggtc gaagctgctg gggtgttgga gagcttgatg acaagagagc
     2281 tcagcagagg tttcagttca gtctacagga cttcaggtgc tgtgctgtct tggggagagg
     2341 acactttgga aaggtgctgt tggcggaata taaacacaca aatgaaatgt ttgcaataaa
     2401 agccttaaag aaaggagaca ttgtggcacg agatgaagta gacagcctga tgtgtgaaaa
     2461 aagaattttt gagactgtga atagtgtcag gcacccgttt ctagtgaacc tttttgcatg
     2521 tttccaaaca aaagaacatg tttgcttcgt gatggagtat gctgcaggtg gtgacctgat
     2581 gatgcacatc catactgatg tcttctctga acccagagct gtattttatg cagcatgtgt
     2641 agttcttggg ttgcaatact tacatgaaca caaaattgtt tatagagatt tgaagttgga
     2701 taacttgttg ctagatacag aaggctttgt gaaaattgct gactttggtc tttgcaaaga
     2761 aggaatgggg tatggagata ggacaagcac attttgtggt actcctgagt ttcttgcccc
     2821 agaagtccta acagaaacat cgtacacaag ggctgtagat tggtggggcc ttggtgtact
     2881 tatatatgag atgctcgtcg gcgagtctcc ctttcctggt gatgatgaag aagaggtttt
     2941 tgacagtatt gtaaatgacg aagtaaggta tccaaggttc ttatctacag aagccatttc
     3001 cataatgaga aggctgctaa ggagaaatcc tgagcggcgc ctgggagctg gtgagaagga
     3061 tgcagaggat gtcaagaagc acccgttttt ccggctaacc gactggagtg ctctaatgga
     3121 caaaaaagta aagccaccat ttgtacctac cattagagga cgggaagatg ttagtaactt
     3181 tgatgatgaa tttacctcag aagcacctat tctgactcca cctcgagaac caaggatact
     3241 cttggaagag gagcaggaga tgttccacga ttttgactac gttgctgatt ggtgttaagt
     3301 ttctagaccc tgagaccaag cagactgacc aacaagaaga cctcttgaaa acagcagccc
     3361 tgcatttcct ttccgtgcca ccaggagctt ctgagattat ataaagcaca tgaagatgtg
     3421 gttacagtac tgttttatac cttaatgttt tcaacgtaaa tctgaacact ggaagcagtt
     3481 tcatggagtt taggctgagt gaacattggc catggaaatc cattacaaat aaatactttt
     3541 acatcaatac tcttattttt aaaaagaaga agaaccactt ttttatagtc cccatgtttg
     3601 ccctaggcct gccttaagtg gaaggaagat taatcactta actatatttt acaaaagaaa
     3661 aaatagggct ttggatgcta ttactcgtaa tataaaaagt gtgattctat ttcactaagg
     3721 caattatttt tttaagaaat cactataata tgaaaaaatg tggcagtttg tacacctttg
     3781 gtggcttgat ttaaagaaaa gaatgagaat gtattgatgt ctatttgatg aattttctat
     3841 atttatcagg agaacacttt attgccttaa gtttaccaac catgtagcag agcctcctag
     3901 ctttccagca gagctacctg ccaaatgagt ttgtgtatta aacaatgctg caaaaagata
     3961 gggacatcag cattcactta acagtaacaa tgaggacttc taagcagctg gaaccaagat
     4021 accatgctaa ctctatgcat tcccaaagtc aaggcaccgt ggaggatgtt cagtcaggtc
     4081 ttccaaagtc agtgaacctg attaaccctt tattgatatg cattctacac gtatttcaac
     4141 ctagacatct tgttcttgtg tctgctgtag aaggaaactc tatctttgtt aaaccataga
     4201 gattatcatg gtatacatgc tgtgaacatg tatatgtaca agttgtaatg tattctgtgt
     4261 tgtaggcata ttatttaatt tcaccacgtc atcacttttg tacagtggcc aagcagacac
     4321 ctccaactcc agcatttcca ttaagtgcat ttgtacattt taggccgctg gatccagatt
     4381 ttatattggc agttactgag ggctaaagca atatattgta acagaatgta taaatatttt
     4441 tgataaaaca gtctatattt tataaaaagt tattataaca ctaaagctgt acctcaaaag
     4501 tctcacaaat aatttgatca aatgctctac gcatcctcag aggatccgtt cctggctttg
     4561 tatcgctctt accatatgcc tgtttgcaaa tcatgatctt cactgggctg gaattttatt
     4621 agatttttgt tttgtttttc ccttttttct cctaggctgt cactctgttg ttctccctca
     4681 gttcagtaat atcaataact ctttgcttag catgttaggg ccatgatacc tcaggaacag
     4741 cagaatggta acaaccacac tcgaattaac catgtaatca cagtgagctc atctcttaca
     4801 attttccaaa tctgcgttat tgagaagcag gaaagtacac aaataatgaa gagttgagct
     4861 tgaaacagct ttgctgacat acattggcct gttgttattg tactctgaga atgattacaa
     4921 ttgtccagag attctttata ttaccttcca aattcattat tatttttcac ttaagatcct
     4981 ttggaagaaa attttctaaa aagagaagca atatgttgct gtaaattgct ttaaaatgcc
     5041 agttagttgc cttggaagag aacaatagaa aaaaaaaaaa tagcgtaatt gtctagcttt
     5101 aattgaccag tgttaactca gtctgaaaat aaaacactaa ctacttttga ggtacagtct
     5161 gcataccttt gttggttctg aaatatgcat agaaagcaac ttttcaaatg ggcttttagc
     5221 attaaaaact gacatagagt tatatacaca gtgtacaaaa atttttttaa ttgaagtcaa
     5281 tcattaaaaa atttagaggt cagggcatag tcacacaatt atgctgaaaa aaaaagcact
     5341 catttttctg tagtttaaat atatatatat attttagcca gattttaaat tttcagagtg
     5401 taaatagatt ttgctattac tgtatgtgta ttgactgctc aagcactttg taaggaaagt
     5461 ggatacttta gaatggtacc ctatgcatcc aaatgaaatg tgcctttaca tcattctaag
     5521 aaaaaggggg ttttgcagtc ataaattacc acaaatgcac aaattaaagt ttaaaataga
     5581 ttgaaatttg taattttggt tttaagtttt cattcctttt agaattgtcc ttgtgttaaa
     5641 atagttggct aaaaacgtta gctctacttg gaggtctcaa gaaaattatt tttagttagc
     5701 tttcttttca tcctggagaa ggcacattgt ccactttact tggaataatg gggagatttc
     5761 atctaggtct gtattctaca gcttctttca tccatgcaca gaatggccac tgtcactaga
     5821 caccaagacc aggaaagttg ccaggaggag agaagcaatg atgtttccaa gactaaaaag
     5881 caaatatgct aatgtaaagt gtaacatggg agcttcctcg tgttagagca gtggctctca
     5941 tcctaggggg tatgagaggg gttagaatgg ccacttcata ggagctgcat atcagatgct
     6001 tatattgcaa tttataatag tagcaaaatt acagatatga agtagcaaca agataccgtt
     6061 aaggttaagg gtcaccacaa catgaggaac tgtattaaag ggccgaaaca ttaggaactt
     6121 tgagaaccac tgtgttagag ggtaacattt tagtattgga tctaacattt gagaaaattg
     6181 tctaattaat aaagcagaat gctagcc
//
