LOCUS       NM_177262               3436 bp    mRNA    linear   ROD 17-SEP-2024
DEFINITION  Mus musculus protein kinase N1 (Pkn1), transcript variant 2, mRNA.
ACCESSION   NM_177262
VERSION     NM_177262.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Yoshida,S., Yoshida,T., Inukai,K., Kato,K., Yura,Y., Hattori,T.,
            Enomoto,A., Ohashi,K., Okumura,T., Ouchi,N., Kawase,H.,
            Wettschureck,N., Offermanns,S., Murohara,T. and Takefuji,M.
  TITLE     Protein kinase N promotes cardiac fibrosis in heart failure by
            fibroblast-to-myofibroblast conversion
  JOURNAL   Nat Commun 15 (1), 7638 (2024)
   PUBMED   39266515
  REMARK    GeneRIF: Protein kinase N promotes cardiac fibrosis in heart
            failure by fibroblast-to-myofibroblast conversion.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 3436)
  AUTHORS   Zur Nedden,S., Safari,M.S., Fresser,F., Faserl,K., Lindner,H.,
            Sarg,B., Baier,G. and Baier-Bitterlich,G.
  TITLE     PKN1 Exerts Neurodegenerative Effects in an In Vitro Model of
            Cerebellar Hypoxic-Ischemic Encephalopathy via Inhibition of
            AKT/GSK3beta Signaling
  JOURNAL   Biomolecules 13 (11), 1599 (2023)
   PUBMED   38002281
  REMARK    GeneRIF: PKN1 Exerts Neurodegenerative Effects in an In Vitro Model
            of Cerebellar Hypoxic-Ischemic Encephalopathy via Inhibition of
            AKT/GSK3beta Signaling.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3436)
  AUTHORS   Wang,D., Shang,Q., Mao,J., Gao,C., Wang,J., Wang,D., Wang,H.,
            Jia,H., Peng,P., Du,M., Luo,Z. and Yang,L.
  TITLE     Phosphorylation of KRT8 (keratin 8) by excessive mechanical
            load-activated PKN (protein kinase N) impairs autophagosome
            initiation and contributes to disc degeneration
  JOURNAL   Autophagy 19 (9), 2485-2503 (2023)
   PUBMED   36897022
  REMARK    GeneRIF: Phosphorylation of KRT8 (keratin 8) by excessive
            mechanical load-activated PKN (protein kinase N) impairs
            autophagosome initiation and contributes to disc degeneration.
REFERENCE   5  (bases 1 to 3436)
  AUTHORS   Marshall,J.J.T., Cull,J.J., Alharbi,H.O., Zaw Thin,M.,
            Cooper,S.T.E., Barrington,C., Vanyai,H., Snoeks,T., Siow,B.,
            Suaarez-Bonnet,A., Herbert,E., Stuckey,D.J., Cameron,A.J.M.,
            Prin,F., Cook,A.C., Priestnall,S.L., Chotani,S., Rackham,O.J.L.,
            Meijles,D.N., Mohun,T., Clerk,A. and Parker,P.J.
  TITLE     PKN2 deficiency leads both to prenatal 'congenital' cardiomyopathy
            and defective angiotensin II stress responses
  JOURNAL   Biochem J 479 (13), 1467-1486 (2022)
   PUBMED   35730579
REFERENCE   6  (bases 1 to 3436)
  AUTHORS   Ballif,B.A., Villen,J., Beausoleil,S.A., Schwartz,D. and Gygi,S.P.
  TITLE     Phosphoproteomic analysis of the developing mouse brain
  JOURNAL   Mol Cell Proteomics 3 (11), 1093-1101 (2004)
   PUBMED   15345747
REFERENCE   7  (bases 1 to 3436)
  AUTHORS   Torbett,N.E., Casamassima,A. and Parker,P.J.
  TITLE     Hyperosmotic-induced protein kinase N 1 activation in a vesicular
            compartment is dependent upon Rac1 and 3-phosphoinositide-dependent
            kinase 1
  JOURNAL   J Biol Chem 278 (34), 32344-32351 (2003)
   PUBMED   12783890
REFERENCE   8  (bases 1 to 3436)
  AUTHORS   Duan,W., Sun,B., Li,T.W., Tan,B.J., Lee,M.K. and Teo,T.S.
  TITLE     Cloning and characterization of AWP1, a novel protein that
            associates with serine/threonine kinase PRK1 in vivo
  JOURNAL   Gene 256 (1-2), 113-121 (2000)
   PUBMED   11054541
REFERENCE   9  (bases 1 to 3436)
  AUTHORS   Bartsch,J.W., Mukai,H., Takahashi,N., Ronsiek,M., Fuchs,S.,
            Jockusch,H. and Ono,Y.
  TITLE     The protein kinase N (PKN) gene PRKCL1/Prkcl1 maps to human
            chromosome 19p12-p13.1 and mouse chromosome 8 with close linkage to
            the myodystrophy (myd) mutation
  JOURNAL   Genomics 49 (1), 129-132 (1998)
   PUBMED   9570957
REFERENCE   10 (bases 1 to 3436)
  AUTHORS   Batshake,B. and Sundelin,J.
  TITLE     The mouse genes for the EP1 prostanoid receptor and the PKN protein
            kinase overlap
  JOURNAL   Biochem Biophys Res Commun 227 (1), 70-76 (1996)
   PUBMED   8858105
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC164432.3, BC052923.1 and CX568360.1.
            
            On Dec 9, 2010 this sequence version replaced NM_177262.3.
            
            Transcript Variant: This variant (2) contains a different segment
            for its 5' UTR and 5' coding region, compared to variant 1. The
            resulting protein (isoform 2) has a shorter and distinct N-terminus
            when it is compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC052923.1, AK170556.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-53                AC164432.3         69350-69402
            54-3102             BC052923.1         1-3049
            3103-3436           CX568360.1         467-800
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 40.22 cM"
     gene            1..3436
                     /gene="Pkn1"
                     /gene_synonym="DBK; F730027O18Rik; PAK1; Pkn; PRK1;
                     Prkcl1; Stk3"
                     /note="protein kinase N1"
                     /db_xref="GeneID:320795"
                     /db_xref="MGI:MGI:108022"
     exon            1..213
                     /gene="Pkn1"
                     /gene_synonym="DBK; F730027O18Rik; PAK1; Pkn; PRK1;
                     Prkcl1; Stk3"
                     /inference="alignment:Splign:2.1.0"
     CDS             193..3033
                     /gene="Pkn1"
                     /gene_synonym="DBK; F730027O18Rik; PAK1; Pkn; PRK1;
                     Prkcl1; Stk3"
                     /EC_number="2.7.11.13"
                     /note="isoform 2 is encoded by transcript variant 2;
                     protein kinase C-like 1; serine/threonine-protein kinase
                     N1; serine/threonine kinase 3; protein kinase C-like PKN;
                     protein-kinase C-related kinase 1; serine-threonine
                     protein kinase N"
                     /codon_start=1
                     /product="serine/threonine-protein kinase N1 isoform 2"
                     /protein_id="NP_796236.2"
                     /db_xref="CCDS:CCDS22459.1"
                     /db_xref="GeneID:320795"
                     /db_xref="MGI:MGI:108022"
                     /translation="MAGDAVQSEPRSWSLLEQLGLAGADLAAPGVQQQLELERERLKR
                     EIRKELKLKEGAENLRRATTDLGRSLAPVELLLRGSARRLDLLHQQLQELHAHVVLPD
                     PAAGSDATQSLAEGSPICSSTNLSRVAGLEKQLAIELKVKQGAENMIQTYSNGSSKDR
                     KLLLTAQQMLQDSKTKIDIIRMQLRRALQALQAGELESQAAPDEAQGDPELGAVELRI
                     EELRHHFRVEHAVAEGAKNVLRLLSGAKAPDRKAVSEAQEKLTESNQKLGLLRESLER
                     RLGELPADHPKGRLLREELTAASSSAFSAILPGPFPATHYSTLSKPAPLTGTLEVRVV
                     GCKNLPETIPWSPPPSVGASGTPESRTPFLSRPARGLYSRSGSLSGRSSLRGEAENAT
                     EVSTVLKLDNTVVGQTAWKPCGPNAWDQSFTLELERARELELAVFWRDQRGLCALKFL
                     KLEDFLDNERHEVQLDMEPQGCLVAEVTFRNPIIERIPRLQRQKKIFSKQQGKAFQRA
                     RQMNIDVATWVRLLRRLIPSAVATGTFSPNASPGAEIRHTGDISMEKLNLGADSDSSS
                     QKSPPGLPSTSCSLSSPTHESTTSPELPSETQETPGPGLCSPLRKSPLTLEDFKFLAV
                     LGRGHFGKVLLSEFRSSGELFAIKALKKGDIVARDEVESLMCEKRILAAVTRAGHPFL
                     VNLFGCFQTPEHVCFVMEYSAGGDLMLHIHSDVFSEPRAVFYSACVVLGLQFLHEHKI
                     VYRDLKLDNLLLDTEGYVKIADFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTDTSYTR
                     AVDWWGLGVLLYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSAEAIGIMRRLLRR
                     NPERRLGSTERDAEDVKKQPFFRSLGWDVLLARRLPPPFVPTLSGRTDVSNFDEEFTG
                     EAPTLSPPRDARPLTAAEQAAFRDFDFVAGGY"
     exon            214..514
                     /gene="Pkn1"
                     /gene_synonym="DBK; F730027O18Rik; PAK1; Pkn; PRK1;
                     Prkcl1; Stk3"
                     /inference="alignment:Splign:2.1.0"
     exon            515..666
                     /gene="Pkn1"
                     /gene_synonym="DBK; F730027O18Rik; PAK1; Pkn; PRK1;
                     Prkcl1; Stk3"
                     /inference="alignment:Splign:2.1.0"
     exon            667..811
                     /gene="Pkn1"
                     /gene_synonym="DBK; F730027O18Rik; PAK1; Pkn; PRK1;
                     Prkcl1; Stk3"
                     /inference="alignment:Splign:2.1.0"
     exon            812..957
                     /gene="Pkn1"
                     /gene_synonym="DBK; F730027O18Rik; PAK1; Pkn; PRK1;
                     Prkcl1; Stk3"
                     /inference="alignment:Splign:2.1.0"
     exon            958..1171
                     /gene="Pkn1"
                     /gene_synonym="DBK; F730027O18Rik; PAK1; Pkn; PRK1;
                     Prkcl1; Stk3"
                     /inference="alignment:Splign:2.1.0"
     exon            1172..1366
                     /gene="Pkn1"
                     /gene_synonym="DBK; F730027O18Rik; PAK1; Pkn; PRK1;
                     Prkcl1; Stk3"
                     /inference="alignment:Splign:2.1.0"
     exon            1367..1476
                     /gene="Pkn1"
                     /gene_synonym="DBK; F730027O18Rik; PAK1; Pkn; PRK1;
                     Prkcl1; Stk3"
                     /inference="alignment:Splign:2.1.0"
     exon            1477..1620
                     /gene="Pkn1"
                     /gene_synonym="DBK; F730027O18Rik; PAK1; Pkn; PRK1;
                     Prkcl1; Stk3"
                     /inference="alignment:Splign:2.1.0"
     exon            1621..1696
                     /gene="Pkn1"
                     /gene_synonym="DBK; F730027O18Rik; PAK1; Pkn; PRK1;
                     Prkcl1; Stk3"
                     /inference="alignment:Splign:2.1.0"
     exon            1697..1838
                     /gene="Pkn1"
                     /gene_synonym="DBK; F730027O18Rik; PAK1; Pkn; PRK1;
                     Prkcl1; Stk3"
                     /inference="alignment:Splign:2.1.0"
     exon            1839..1932
                     /gene="Pkn1"
                     /gene_synonym="DBK; F730027O18Rik; PAK1; Pkn; PRK1;
                     Prkcl1; Stk3"
                     /inference="alignment:Splign:2.1.0"
     exon            1933..2012
                     /gene="Pkn1"
                     /gene_synonym="DBK; F730027O18Rik; PAK1; Pkn; PRK1;
                     Prkcl1; Stk3"
                     /inference="alignment:Splign:2.1.0"
     exon            2013..2088
                     /gene="Pkn1"
                     /gene_synonym="DBK; F730027O18Rik; PAK1; Pkn; PRK1;
                     Prkcl1; Stk3"
                     /inference="alignment:Splign:2.1.0"
     exon            2089..2180
                     /gene="Pkn1"
                     /gene_synonym="DBK; F730027O18Rik; PAK1; Pkn; PRK1;
                     Prkcl1; Stk3"
                     /inference="alignment:Splign:2.1.0"
     exon            2181..2357
                     /gene="Pkn1"
                     /gene_synonym="DBK; F730027O18Rik; PAK1; Pkn; PRK1;
                     Prkcl1; Stk3"
                     /inference="alignment:Splign:2.1.0"
     exon            2358..2420
                     /gene="Pkn1"
                     /gene_synonym="DBK; F730027O18Rik; PAK1; Pkn; PRK1;
                     Prkcl1; Stk3"
                     /inference="alignment:Splign:2.1.0"
     exon            2421..2497
                     /gene="Pkn1"
                     /gene_synonym="DBK; F730027O18Rik; PAK1; Pkn; PRK1;
                     Prkcl1; Stk3"
                     /inference="alignment:Splign:2.1.0"
     exon            2498..2640
                     /gene="Pkn1"
                     /gene_synonym="DBK; F730027O18Rik; PAK1; Pkn; PRK1;
                     Prkcl1; Stk3"
                     /inference="alignment:Splign:2.1.0"
     exon            2641..2748
                     /gene="Pkn1"
                     /gene_synonym="DBK; F730027O18Rik; PAK1; Pkn; PRK1;
                     Prkcl1; Stk3"
                     /inference="alignment:Splign:2.1.0"
     exon            2749..2829
                     /gene="Pkn1"
                     /gene_synonym="DBK; F730027O18Rik; PAK1; Pkn; PRK1;
                     Prkcl1; Stk3"
                     /inference="alignment:Splign:2.1.0"
     exon            2830..3436
                     /gene="Pkn1"
                     /gene_synonym="DBK; F730027O18Rik; PAK1; Pkn; PRK1;
                     Prkcl1; Stk3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3077..3082
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pkn1"
                     /gene_synonym="DBK; F730027O18Rik; PAK1; Pkn; PRK1;
                     Prkcl1; Stk3"
                     /note="hexamer: AACAAG"
     polyA_site      3078
                     /gene="Pkn1"
                     /gene_synonym="DBK; F730027O18Rik; PAK1; Pkn; PRK1;
                     Prkcl1; Stk3"
     polyA_site      3108
                     /gene="Pkn1"
                     /gene_synonym="DBK; F730027O18Rik; PAK1; Pkn; PRK1;
                     Prkcl1; Stk3"
                     /note="major polyA site"
ORIGIN      
        1 gtgacccacc ctgcagccgc tggggccgga gggcgcagct cggcggaggc ctggccaggc
       61 cgggccgagc gcagcaggcc ggagcgcgcc gcgcgcctgg gagggtctgg gcgccgcgcc
      121 tcccgccgca cccgcccgcg cgctcgcaag cccttccttg gcgctgggac ccctggcggg
      181 cggccggagg acatggccgg cgacgccgta cagagtgagc ctcgaagctg gtcgctgctg
      241 gaacagctgg gtctggctgg ggctgacctg gcagcccccg gggtgcagca acagctggag
      301 ttagagcgag agcgactgaa gcgggagata cggaaggagc tgaagctgaa ggagggcgct
      361 gagaacctga ggcgagccac cactgacctg ggccgtagcc tggcccctgt ggagctgctg
      421 ctgcggggct ccgctcgacg cctagactta ctgcaccagc agctgcagga gctgcatgca
      481 catgtggtgc tgcctgaccc tgcagctgga agcgatgcta cccaatccct tgcagagggc
      541 agccctatct gctcatccac caacctgagc agagtggctg ggctggagaa gcagctggcc
      601 attgagctca aggtcaaaca gggggcagag aacatgatcc agacctatag caatggcagc
      661 agcaaggacc ggaagctgtt gttgacagcc cagcagatgc tgcaggacag taagaccaag
      721 attgacatca tccgcatgca gcttcgaaga gcgctccagg cactacaggc tggagagctg
      781 gagagtcagg cagctcctga tgaagcccaa ggagatccag aactgggagc cgtagagcta
      841 cgcattgagg agctacgaca ccattttcga gtggagcatg cagtggcaga aggtgccaag
      901 aatgtcctgc gcctgctcag tggggcaaag gccccagacc gcaaggcagt cagcgaggct
      961 caggagaaat tgactgagtc caaccagaag ctgggcttgc tgcgggaatc actggagagg
     1021 cgccttgggg agttgcctgc cgatcacccc aaggggcggc tgcttcggga ggagctcact
     1081 gcagcctcgt cctcagcctt cagcgccata ctgcctgggc ccttccctgc cactcactac
     1141 agcaccttga gcaagcctgc accgctcaca gggaccctgg aagtacgagt ggtgggctgc
     1201 aaaaaccttc ccgagaccat cccctggagc cctcccccct cagtgggggc atctgggacc
     1261 cccgaaagcc gcactccgtt cctgagtcgc cccgctcggg gcctttacag ccggagtgga
     1321 agccttagtg gacggagcag cctcagaggg gaggcagaga atgccactga ggtcagcacc
     1381 gtgctcaagc tggacaacac agtggtgggg caaacagcct ggaagccatg cggccccaat
     1441 gcctgggacc agagctttac cctggagctg gagagggctc gggagctgga gctggctgtg
     1501 ttctggcgag accagagggg tctgtgtgct ctcaaatttc tgaagttgga ggacttcttg
     1561 gacaacgaga ggcatgaggt gcagctggac atggaacccc agggctgcct ggtggctgag
     1621 gtcactttcc gaaaccccat catcgagcgg atccctaggc tccaaaggca gaaaaaaatc
     1681 ttctccaagc agcaagggaa ggcatttcag cgagccagac agatgaacat agatgtggcg
     1741 acttgggtgc ggctgctccg tagactcatc cctagtgctg tggccactgg caccttcagt
     1801 cccaatgcat ctccaggtgc tgagatccgg cacactggag acatatccat ggagaaattg
     1861 aatctcggtg ctgactcaga cagctcgtcc caaaagagcc caccagggct gccctccacc
     1921 tcatgcagcc tgagttctcc aacccatgaa tccaccacat ctccagagct gccttcagag
     1981 acccaggaga ctccaggccc tggcctgtgc agccccttga gaaagtcgcc cctgacactt
     2041 gaggacttca agttcctggc cgtgcttggc cggggtcact ttggaaaggt gctgctgtct
     2101 gaattccgct ccagtgggga gctctttgcc atcaaagcct tgaagaaagg tgacattgta
     2161 gcccgagatg aggttgagag cctgatgtgt gagaagcgga ttttggcggc cgtgaccagg
     2221 gcaggacatc ccttcctggt gaaccttttc ggctgtttcc agaccccaga gcacgtgtgc
     2281 tttgtgatgg agtactcggc gggtggagac ctgatgctgc acattcatag cgacgtgttc
     2341 tcagagcctc gggctgtctt ctattcggcc tgtgtggtgc tgggactgca gttcctccat
     2401 gaacacaaga ttgtctacag ggacctgaag ttggacaatt tgctcctgga tactgagggc
     2461 tacgtcaaga tcgcagactt tggcctctgc aaggagggga tgggctatgg ggaccggacc
     2521 agcacgttct gcggaactcc ggagttcctg gcgccggaag tgctcacaga cacatcctac
     2581 acgcgagcag tggactggtg gggactgggc gtgctgctct atgagatgtt ggttggagag
     2641 tctccgttcc ctggggatga tgaggaggag gtatttgaca gcattgtcaa cgacgaagtt
     2701 cgctatcccc gcttcctgtc tgcagaggcc atcggcatca tgagaaggct actgcggagg
     2761 aacccggagc ggaggctggg gtccactgag cgcgatgcag aagatgtgaa aaaacagcct
     2821 ttcttccggt ctctgggctg ggatgtcctg ctggcccgcc gcttgcctcc acccttcgtg
     2881 cctacacttt cagggcgcac agatgtcagc aacttcgatg aggagttcac tggggaggcc
     2941 cccacactga gtcctccccg ggatgcacgg cccctcacag ctgcggagca ggcagccttc
     3001 cgggatttcg actttgtggc cggaggctac tagccccaag cccctgcctt acccaagagt
     3061 tcttgatttt ttaaaaaaca agcctttggg gtttactcca tacatgcatt ttcagcctct
     3121 gtgtgcatct ggactggagt gtgcttggag caggtgacag aggcaaggca gactcttggg
     3181 ggcaggactg atggggcaca tttgaaaggt caagatggga acatgcagta gtgggcttag
     3241 gggtatgttg gagagcattg gggttttaag gccgtgtggg tagaaggtgt tgagattctt
     3301 gggatagttt gggatagacg cttgcaagag tctattgggg tggtgacaga gatgggaagt
     3361 gaatccaaaa ctttgctcta tttatggaga gcccgaatct tagaactaag gttgcggcgc
     3421 tgtgcgcatg ctcttc
//
