LOCUS       NM_013631               2787 bp    mRNA    linear   ROD 07-FEB-2025
DEFINITION  Mus musculus pyruvate kinase liver and red blood cell (Pklr),
            transcript variant 1, mRNA.
ACCESSION   NM_013631
VERSION     NM_013631.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Oelkrug,R., Harder,L., Pedaran,M., Hoffmann,A., Kolms,B.,
            Inderhees,J., Gachkar,S., Resch,J., Johann,K., Johren,O., Krause,K.
            and Mittag,J.
  TITLE     Maternal thyroid hormone receptor beta activation in mice sparks
            brown fat thermogenesis in the offspring
  JOURNAL   Nat Commun 14 (1), 6742 (2023)
   PUBMED   37875497
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Rong,R., Wei,Y., Li,L., Wang,T., Zhu,H., Xiao,G. and Wang,Y.
  TITLE     Image-based quantification of histological features as a function
            of spatial location using the Tissue Positioning System
  JOURNAL   EBioMedicine 94, 104698 (2023)
   PUBMED   37453365
REFERENCE   3  (bases 1 to 2787)
  AUTHORS   Zhang,C., Shi,M., Kim,W., Arif,M., Klevstig,M., Li,X., Yang,H.,
            Bayram,C., Bolat,I., Tozlu,O.O., Hacimuftuoglu,A., Yildirim,S.,
            Sebhaoui,J., Iqbal,S., Wei,Y., Shi,X., Nielsen,J., Turkez,H.,
            Uhlen,M., Boren,J. and Mardinoglu,A.
  TITLE     Discovery of therapeutic agents targeting PKLR for NAFLD using drug
            repositioning
  JOURNAL   EBioMedicine 83, 104214 (2022)
   PUBMED   35988463
  REMARK    Erratum:[EBioMedicine. 2024 Jul;105:105224. doi:
            10.1016/j.ebiom.2024.105224.. PMID: 38943726]
REFERENCE   4  (bases 1 to 2787)
  AUTHORS   Monica Shih,M.C., Huang,C.C., Chu,H.P., Hsu,N.C. and Chung,B.C.
  TITLE     Embryonic Steroids Control Developmental Programming of Energy
            Balance
  JOURNAL   Endocrinology 162 (12) (2021)
   PUBMED   34599818
REFERENCE   5  (bases 1 to 2787)
  AUTHORS   Wei,Y., Wang,Y.G., Jia,Y., Li,L., Yoon,J., Zhang,S., Wang,Z.,
            Zhang,Y., Zhu,M., Sharma,T., Lin,Y.H., Hsieh,M.H., Albrecht,J.H.,
            Le,P.T., Rosen,C.J., Wang,T. and Zhu,H.
  TITLE     Liver homeostasis is maintained by midlobular zone 2 hepatocytes
  JOURNAL   Science 371 (6532) (2021)
   PUBMED   33632817
  REMARK    Erratum:[Science. 2021 Sep 3;373(6559):eabl8195. doi:
            10.1126/science.abl8195.. PMID: 34516857]
REFERENCE   6  (bases 1 to 2787)
  AUTHORS   Serikawa,T., Kuramoto,T., Hilbert,P., Mori,M., Yamada,J.,
            Dubay,C.J., Lindpainter,K., Ganten,D., Guenet,J.L., Lathrop,G.M. et
            al.
  TITLE     Rat gene mapping using PCR-analyzed microsatellites
  JOURNAL   Genetics 131 (3), 701-721 (1992)
   PUBMED   1628813
REFERENCE   7  (bases 1 to 2787)
  AUTHORS   Dower,N.A., Seldin,M.F., Pugh,S. and Stone,J.C.
  TITLE     Organization and chromosomal locations of Rap1a/Krev sequences in
            the mouse
  JOURNAL   Mamm Genome 3 (3), 162-167 (1992)
   PUBMED   1617222
REFERENCE   8  (bases 1 to 2787)
  AUTHORS   Oakey,R.J., Howard,T.A., Hogarth,P.M., Tani,K. and Seldin,M.F.
  TITLE     Chromosomal mapping of the high affinity Fc gamma receptor gene
  JOURNAL   Immunogenetics 35 (4), 279-282 (1992)
   PUBMED   1347284
REFERENCE   9  (bases 1 to 2787)
  AUTHORS   Fitton,L.A., Davidson,M., Moore,K.J., Charles,D.J., Pretsch,W.,
            Elston,R.C. and Bulfield,G.
  TITLE     The liver/erythrocyte pyruvate kinase gene complex [Pk-1] in the
            mouse: regulatory gene mutations
  JOURNAL   Genet Res 58 (3), 233-241 (1991)
   PUBMED   1802805
REFERENCE   10 (bases 1 to 2787)
  AUTHORS   Bessis,A., Simon-Chazottes,D., Devillers-Thiery,A., Guenet,J.L. and
            Changeux,J.P.
  TITLE     Chromosomal localization of the mouse genes coding for alpha 2,
            alpha 3, alpha 4 and beta 2 subunits of neuronal nicotinic
            acetylcholine receptor
  JOURNAL   FEBS Lett 264 (1), 48-52 (1990)
   PUBMED   2338144
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY781095.1, BY107231.1, AK149508.1 and AK149524.1.
            
            On Jul 25, 2007 this sequence version replaced NM_013631.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1). Isoform 1 is also
            known as isoform R.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D63764.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849388, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-96                BY781095.1         1-96
            97-141              BY107231.1         65-109
            142-2547            AK149508.1         17-2422
            2548-2787           AK149524.1         2421-2660
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 39.01 cM"
     gene            1..2787
                     /gene="Pklr"
                     /gene_synonym="Pk-1; Pk1; R-PK"
                     /note="pyruvate kinase liver and red blood cell"
                     /db_xref="GeneID:18770"
                     /db_xref="MGI:MGI:97604"
     exon            1..141
                     /gene="Pklr"
                     /gene_synonym="Pk-1; Pk1; R-PK"
                     /inference="alignment:Splign:2.1.0"
     CDS             42..1766
                     /gene="Pklr"
                     /gene_synonym="Pk-1; Pk1; R-PK"
                     /EC_number="2.7.1.40"
                     /note="isoform 1 is encoded by transcript variant 1;
                     pyruvate kinase isozymes R/L; L-PK; pyruvate kinase
                     isozymes L/R; pyruvate kinase PKLR"
                     /codon_start=1
                     /product="pyruvate kinase PKLR isoform 1"
                     /protein_id="NP_038659.2"
                     /db_xref="CCDS:CCDS17489.1"
                     /db_xref="GeneID:18770"
                     /db_xref="MGI:MGI:97604"
                     /translation="MSVQENELPQQLWPWIFKSQKDLAKSALSGAPGGPAGYLRRASV
                     AQLTQELGTAFFQQQQLPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSVD
                     RLKEMIKAGMNIARLNFSHGSHEYHAESIANIREAAESFATSPLSYRPVAIALDTKGP
                     EIRTGVLQGGPESEVEIVKGSQVLVTVDPKFRTRGDAKTVWVDYHNITQVVAVGGRIY
                     IDDGLISLVVRKIGPEGLVTEVEHGGFLGNRKGVNLPNAEVDLPGLSEQDLLDLRFGV
                     EHNVDIIFASFVRKASDVVAVRDALGPEGRGIKIISKIENHEGVKKFDEILEVSDGIM
                     VARGDLGIEIPAEKVFLAQKMMIGRCNLAGKPVVCATQMLESMITKARPTRAETSDVA
                     NAVLDGADCIMLSGETAKGSFPVEAVKMQHAIAREAEAAVYHRQLFEELRRAAPLSRD
                     PTEVTAIGAVEASFKCCAAAIIVLTKTGRSAQLLSRYRPRAAVIAVTRSAQAARQVHL
                     SRGVFPLLYREPPEAVWADDVDRRVQFGIESGKLRGFLRVGDLVIVVTGWRPGSGYTN
                     IMRVLTIS"
     exon            142..324
                     /gene="Pklr"
                     /gene_synonym="Pk-1; Pk1; R-PK"
                     /inference="alignment:Splign:2.1.0"
     exon            325..416
                     /gene="Pklr"
                     /gene_synonym="Pk-1; Pk1; R-PK"
                     /inference="alignment:Splign:2.1.0"
     exon            417..548
                     /gene="Pklr"
                     /gene_synonym="Pk-1; Pk1; R-PK"
                     /inference="alignment:Splign:2.1.0"
     exon            549..735
                     /gene="Pklr"
                     /gene_synonym="Pk-1; Pk1; R-PK"
                     /inference="alignment:Splign:2.1.0"
     exon            736..1006
                     /gene="Pklr"
                     /gene_synonym="Pk-1; Pk1; R-PK"
                     /inference="alignment:Splign:2.1.0"
     exon            1007..1157
                     /gene="Pklr"
                     /gene_synonym="Pk-1; Pk1; R-PK"
                     /inference="alignment:Splign:2.1.0"
     exon            1158..1310
                     /gene="Pklr"
                     /gene_synonym="Pk-1; Pk1; R-PK"
                     /inference="alignment:Splign:2.1.0"
     exon            1311..1477
                     /gene="Pklr"
                     /gene_synonym="Pk-1; Pk1; R-PK"
                     /inference="alignment:Splign:2.1.0"
     exon            1478..1659
                     /gene="Pklr"
                     /gene_synonym="Pk-1; Pk1; R-PK"
                     /inference="alignment:Splign:2.1.0"
     exon            1660..2787
                     /gene="Pklr"
                     /gene_synonym="Pk-1; Pk1; R-PK"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2525..2530
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pklr"
                     /gene_synonym="Pk-1; Pk1; R-PK"
                     /note="hexamer: AATGAA"
     polyA_site      2549
                     /gene="Pklr"
                     /gene_synonym="Pk-1; Pk1; R-PK"
                     /note="major polyA site"
ORIGIN      
        1 agtctgtggt tcttgcagcc ccagtcccac actttggaag catgtctgtc caagagaacg
       61 agctacccca gcagctctgg ccctggatct ttaagtccca aaaagactta gcaaagtctg
      121 ctttaagtgg ggctccagga gggccagcag gatacctgag acgtgccagt gtggcccagc
      181 tgacccagga gctgggcact gccttcttcc agcagcagca actgcccgca gctatggcgg
      241 acaccttcct ggaacacctc tgccttctgg atatcgactc agagcctgtg gccgctagga
      301 gcaccagcat cattgccacc atcgggccgg cgtcacgctc tgtggaccgc ctcaaggaga
      361 tgatcaaggc agggatgaac attgcacgac tcaacttctc ccatggctcc catgagtacc
      421 atgcagagtc catcgccaac attcgggagg cggctgaaag ctttgcaacc tccccactca
      481 gctacagacc cgtggccatc gccctggaca ccaagggtcc cgagatacgc actggagtcc
      541 tgcagggggg tccagagtcg gaggtggaaa ttgtgaaggg ttcacaggtg ctggtgactg
      601 tggatccgaa gttccggaca aggggcgatg caaagacagt gtgggtggac taccacaata
      661 tcacccaggt cgttgcagtg gggggccgca tctacattga cgacgggctc atctccttag
      721 tggtgcggaa aattggccca gagggactgg tgaccgaagt ggaacacggt ggtttcttgg
      781 gcaacaggaa gggtgtgaac ttgccaaatg ccgaggtgga cctgcctggg ctatcagagc
      841 aagatctttt ggatctgcgc ttcggggtgg agcataatgt ggacatcatc tttgcctcct
      901 ttgtacgaaa agccagtgat gtggtggcag tccgagatgc cctagggcca gaaggacggg
      961 gcatcaaaat tatcagcaaa atcgagaacc atgaaggcgt gaagaagttt gatgagatcc
     1021 tagaagtgag cgatggcatc atggtggctc ggggtgacct tggcattgag atcccagcag
     1081 agaaggtttt cttggctcag aagatgatga ttggacgctg caacctggct ggcaagcctg
     1141 tcgtttgtgc cacacagatg ctggagagca tgatcactaa ggctcgacca actcgggcgg
     1201 agacaagcga tgtggccaat gctgtgctgg atggggctga ctgtatcatg ctgtctggag
     1261 agaccgccaa aggcagtttc cccgtggagg ctgtaaagat gcaacatgcg attgcccggg
     1321 aggcagaggc cgctgtgtac caccgccagt tgtttgagga gctacgccgg gcagcgccgc
     1381 tgagccgtga cccaaccgag gtcactgcaa ttggagccgt ggaggcttcc ttcaagtgct
     1441 gtgccgcagc catcattgtg ctgacaaaga ctggccgctc agctcagctt ctgtctcgct
     1501 accgacctcg ggctgctgtc attgctgtga ctcgttctgc ccaggctgcc cgacaggtcc
     1561 acctgtcccg aggagtcttc cccttgctct accgtgagcc tccagaggct gtctgggcag
     1621 atgatgtgga ccgaagggtc caatttggca tcgaaagtgg aaagcttcgt ggcttcctcc
     1681 gagttggtga tctggtgatt gtggtgacag gctggcgacc tggctctggc tataccaaca
     1741 ttatgcgggt gctgaccata tcctgaaacg cctctcccct ttctgaccta gttgcacccc
     1801 atttctttca acccacaccc ctcccatagt cccacatctg ccatctagcc acagtctggt
     1861 acttcacaca ggccctgaat gtctgggtcc aattacgcac cggccaccca gcagcatcag
     1921 tcgtatatcc ctgtctcaat ccgctcagct ggactctaag atgccctgag cctttaatcc
     1981 cagcccagct ggttaattcg attcctcctg ggtcctgata attagaacgg gggagtggag
     2041 actgggtaaa cttatccaaa ttccatagaa caattatttt aaaacactct gtcctcggat
     2101 atagccctca tgaccagttc cctagcaaag tgtcacctcc tgatggcctc aagtcagggt
     2161 agaatactcc ttcagggagc acagctccac actttaggga aggctggggt agatgggtgc
     2221 tggagagaac taagctaaac tggctttttt cttttctttt ttttctgata caaggtctca
     2281 ttgtatagct ctgactggcc ccagactcct agaaatcctc ctgcctgacc ctcctgaggg
     2341 ggtaaggact gcaggcatga gtgaccagct ggactttagg tagtcttact ttcttactga
     2401 ctccacaaac catggagccg agcttacgag ccactaatct ggctgttctc ttgcccactg
     2461 atctgctggg tcagatgaaa ttcttccaca accactttgt ccccacatac agatcagaag
     2521 caaaaatgaa tcttttcttt taaacccaac tgtttaggtg caattataaa aaacaactcc
     2581 cacaggcaaa gaatcccaga ctctcccacc caaagagttg tgtggtcctg gccccatgct
     2641 acagtgtact tctgaagggg aacagagcgg gtgggcaggg gattattctt gtccctgaga
     2701 aggtggaggg gctactggcc cacccttcct aggattggat actccaggcc tccctcttct
     2761 tcacaacagt aaacccagat gaggatg
//
