LOCUS       NM_001039391            1053 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus protein kinase inhibitor, gamma (Pkig), transcript
            variant 3, mRNA.
ACCESSION   NM_001039391
VERSION     NM_001039391.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Kurogi,S., Sekimoto,T., Funamoto,T., Ota,T., Nakamura,S., Nagai,T.,
            Nakahara,M., Yoshinobu,K., Araki,K., Araki,M. and Chosa,E.
  TITLE     Development of an efficient screening system to identify novel bone
            metabolism-related genes using the exchangeable gene trap
            mutagenesis mouse models
  JOURNAL   Sci Rep 7, 40692 (2017)
   PUBMED   28106071
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1053)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   4  (bases 1 to 1053)
  AUTHORS   Chen,X., Hausman,B.S., Luo,G., Zhou,G., Murakami,S., Rubin,J. and
            Greenfield,E.M.
  TITLE     Protein kinase inhibitor gamma reciprocally regulates osteoblast
            and adipocyte differentiation by downregulating leukemia inhibitory
            factor
  JOURNAL   Stem Cells 31 (12), 2789-2799 (2013)
   PUBMED   23963683
REFERENCE   5  (bases 1 to 1053)
  AUTHORS   Nakahara,M., Tateyama,H., Araki,M., Nakagata,N., Yamamura,K. and
            Araki,K.
  TITLE     Gene-trap mutagenesis using Mol/MSM-1 embryonic stem cells from
            MSM/Ms mice
  JOURNAL   Mamm Genome 24 (5-6), 228-239 (2013)
   PUBMED   23604909
REFERENCE   6  (bases 1 to 1053)
  AUTHORS   Valerius,M.T. and McMahon,A.P.
  TITLE     Transcriptional profiling of Wnt4 mutant mouse kidneys identifies
            genes expressed during nephron formation
  JOURNAL   Gene Expr Patterns 8 (5), 297-306 (2008)
   PUBMED   18346943
REFERENCE   7  (bases 1 to 1053)
  AUTHORS   Zhao,L., Yang,S., Zhou,G.Q., Yang,J., Ji,D., Sabatakos,G. and
            Zhu,T.
  TITLE     Downregulation of cAMP-dependent protein kinase inhibitor gamma is
            required for BMP-2-induced osteoblastic differentiation
  JOURNAL   Int J Biochem Cell Biol 38 (12), 2064-2073 (2006)
   PUBMED   16870489
  REMARK    GeneRIF: These results indicate that the downregulation of PKIgamma
            may be prerequisite for the PKA activation during the osteoblastic
            differentiation of precursor cells.
REFERENCE   8  (bases 1 to 1053)
  AUTHORS   Chen,X., Dai,J.C., Orellana,S.A. and Greenfield,E.M.
  TITLE     Endogenous protein kinase inhibitor gamma terminates
            immediate-early gene expression induced by cAMP-dependent protein
            kinase (PKA) signaling: termination depends on PKA inactivation
            rather than PKA export from the nucleus
  JOURNAL   J Biol Chem 280 (4), 2700-2707 (2005)
   PUBMED   15557275
  REMARK    GeneRIF: endogenous levels of PKI regulate PKA signaling
REFERENCE   9  (bases 1 to 1053)
  AUTHORS   Wagener,R., Kobbe,B., Aszodi,A., Aeschlimann,D. and Paulsson,M.
  TITLE     Characterization of the mouse matrilin-4 gene: a 5' antiparallel
            overlap with the gene encoding the transcription factor RBP-l
  JOURNAL   Genomics 76 (1-3), 89-98 (2001)
   PUBMED   11549321
REFERENCE   10 (bases 1 to 1053)
  AUTHORS   Collins,S.P. and Uhler,M.D.
  TITLE     Characterization of PKIgamma, a novel isoform of the protein kinase
            inhibitor of cAMP-dependent protein kinase
  JOURNAL   J Biol Chem 272 (29), 18169-18178 (1997)
   PUBMED   9218452
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ051558.1, BY343638.1, AK164716.1 and BY617655.1.
            
            On Aug 11, 2009 this sequence version replaced NM_001039391.1.
            
            Transcript Variant: This variant (3) differs in the 5' UTR,
            compared to variant 4. Variants 1-5 and 7-19 all encode the same
            isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: AK164716.1, BU515168.1 [ECO:0000331]
            RNAseq introns       :: single sample supports all introns
                                    SAMN00849374, SAMN00849375 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-23                CJ051558.1         2-24
            24-383              BY343638.1         1-360
            384-1045            AK164716.1         372-1033
            1046-1053           BY617655.1         391-398
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 84.4 cM"
     gene            1..1053
                     /gene="Pkig"
                     /gene_synonym="PKIgamma"
                     /note="protein kinase inhibitor, gamma"
                     /db_xref="GeneID:18769"
                     /db_xref="MGI:MGI:1343086"
     exon            1..107
                     /gene="Pkig"
                     /gene_synonym="PKIgamma"
                     /inference="alignment:Splign:2.1.0"
     exon            108..177
                     /gene="Pkig"
                     /gene_synonym="PKIgamma"
                     /inference="alignment:Splign:2.1.0"
     exon            178..351
                     /gene="Pkig"
                     /gene_synonym="PKIgamma"
                     /inference="alignment:Splign:2.1.0"
     CDS             201..431
                     /gene="Pkig"
                     /gene_synonym="PKIgamma"
                     /note="isoform 1 is encoded by transcript variant 3;
                     cAMP-dependent protein kinase inhibitor gamma"
                     /codon_start=1
                     /product="cAMP-dependent protein kinase inhibitor gamma
                     isoform 1"
                     /protein_id="NP_001034480.1"
                     /db_xref="CCDS:CCDS17014.1"
                     /db_xref="GeneID:18769"
                     /db_xref="MGI:MGI:1343086"
                     /translation="MMEVESSYSDFISCDRTGRRNAVPDIQGDSEAVSVRKLAGDMGE
                     LALEGAEGQAEGSTPDKEASSQPESSDANTSS"
     exon            352..1053
                     /gene="Pkig"
                     /gene_synonym="PKIgamma"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1025..1030
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pkig"
                     /gene_synonym="PKIgamma"
                     /note="hexamer: AATAAA"
     polyA_site      1048
                     /gene="Pkig"
                     /gene_synonym="PKIgamma"
                     /note="major polyA site"
ORIGIN      
        1 agtcgcgtcg cctaggcaac ggctcggcgg cgggctgcgg gcgccaggcg gccccgggga
       61 ggcgctcgcg ccggcggccg gggctctacg aggcgacaag gcggcagact ttctgcactg
      121 gctaatcaag agtgaaaaac ctgaagagat gcaggcagga aaacggaatt aaactaggcc
      181 tgaggagcga tgagacaggc atgatggaag tcgagtcctc ctactcggac ttcatctcct
      241 gcgaccggac aggccgtcgg aatgcagtcc ctgacatcca gggtgactcg gaggccgtga
      301 gtgtgcggaa gctcgctgga gacatgggcg agctcgcact tgagggagca gaaggacagg
      361 cagagggaag tacccccgac aaggaagcca gcagccagcc tgagagcagt gatgcgaaca
      421 cctcatcctg agtctgcctg tggtcgcaag gctggaagag acctgctgta cctctcggac
      481 tggagccctg gccagccagg cggactgaga gcctcatgga gtcctctgtg gcccccactc
      541 cagccaagtc ctccacccac agcacaggct ccacccttcc ccacctcccc acggtgccca
      601 ggttcactct gaagacacca gcaccatgag atgttattta ttcagctagg cggggctcga
      661 gccaggccct gcccctaggt gagcagccgc cacctggaca gggactgtcc tgtcaccact
      721 caccctccag ccagcctgtc tgtcaggaag atcttcctag ccctttttta aagcctcttt
      781 tacatttttt aaaaacataa ctttttcaga gagagagggg agctgacaat cacttttctt
      841 ctgttttggt ttttggtttt ggttttgttt tgaaagccgt ttaaagctca gttctgatga
      901 tctatgcagc tctaagtttc ctcatggagc ctcacagatc cagttttggg aaaggatttg
      961 gactcaaaac taactgacca attctttgcc ctttgtacca agagactgat acttaggccc
     1021 aggaaataaa tgctggtggt ttgtgtgact ggc
//
